Journal: Frontiers in Medicine

Article Title: Validation of ITPR2 , DPF3 , EPAS1 , and PVT1 -associated SNPs as biomarkers for RCC in an independent case-control cohort

doi: 10.3389/fmed.2026.1734511

Figure Lengend Snippet: Overall survival analysis according to SNP genotypes and metastatic status in RCC patients. (A) Forest plot from the Cox proportional hazards regression model (dominant model). (B) Adjusted survival curves derived from the Cox proportional hazards model comparing overall survival between ITPR2 rs1049380 genotypes (T/T vs. G/T + G/G). (C) Kaplan–Meier survival curves based on observed data for ITPR2 rs1049380, stratified by metastasis status, showing survival probability, cumulative events, and cumulative hazard curves.

Article Snippet: Quantitative polymerase chain reaction (qPCR) was performed with TaqMan TM gene expression assays (Thermo Fisher Scientific, Waltham, MA, United States) for: ITPR2 (Assay ID: Hs00181916_m1), ZEB2 (Assay ID: Hs00207691_m1), MYC (Assay ID: Hs00153408_m1) and PVT1 (Assay ID: Hs00413039_m1). qPCR reactions were performed as follows: 95 °C during 10 min for enzyme activation; followed by 45 cycles of 15 s at 95 °C and 1 min at 60 °C for denaturing and annealing/extension.

Techniques: Derivative Assay

Journal: Frontiers in Medicine

Article Title: Validation of ITPR2 , DPF3 , EPAS1 , and PVT1 -associated SNPs as biomarkers for RCC in an independent case-control cohort

doi: 10.3389/fmed.2026.1734511

Figure Lengend Snippet: Five-year overall survival analysis according to SNPs genotypes and metastatic status in RCC patients. (A) Forest plot from the Cox proportional hazards regression model (dominant model). (B) Adjusted survival curves derived from the Cox proportional hazards model comparing five-years overall survival between ITPR2 rs1049380 and PVT1 rs35252396 genotypes. (C) Kaplan–Meier survival curves based on observed data for ITPR2 rs1049380 and PVT1 rs35252396 stratified by metastasis status, showing survival probability, cumulative events, and cumulative hazard curves.

Article Snippet: Quantitative polymerase chain reaction (qPCR) was performed with TaqMan TM gene expression assays (Thermo Fisher Scientific, Waltham, MA, United States) for: ITPR2 (Assay ID: Hs00181916_m1), ZEB2 (Assay ID: Hs00207691_m1), MYC (Assay ID: Hs00153408_m1) and PVT1 (Assay ID: Hs00413039_m1). qPCR reactions were performed as follows: 95 °C during 10 min for enzyme activation; followed by 45 cycles of 15 s at 95 °C and 1 min at 60 °C for denaturing and annealing/extension.

Techniques: Derivative Assay

Journal: Frontiers in Medicine

Article Title: Validation of ITPR2 , DPF3 , EPAS1 , and PVT1 -associated SNPs as biomarkers for RCC in an independent case-control cohort

doi: 10.3389/fmed.2026.1734511

Figure Lengend Snippet: Expression levels of ITPR2 , PVT1 , and MYC genes by genetic model in tumor and adjacent healthy FFPE tissues.

Article Snippet: Quantitative polymerase chain reaction (qPCR) was performed with TaqMan TM gene expression assays (Thermo Fisher Scientific, Waltham, MA, United States) for: ITPR2 (Assay ID: Hs00181916_m1), ZEB2 (Assay ID: Hs00207691_m1), MYC (Assay ID: Hs00153408_m1) and PVT1 (Assay ID: Hs00413039_m1). qPCR reactions were performed as follows: 95 °C during 10 min for enzyme activation; followed by 45 cycles of 15 s at 95 °C and 1 min at 60 °C for denaturing and annealing/extension.

Techniques: Expressing

Journal: eLife

Article Title: Circadian regulation of endoplasmic reticulum calcium response in cultured mouse astrocytes

doi: 10.7554/eLife.96357

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-ITPR2 , Alomone Labs , Cat# ACC-116; Lot: ACC116AN015; RRID: AB_2340910 , 1:1000.

Techniques: Transfection, Construct, Plasmid Preparation, Sequencing, Negative Control, Software, Microscopy

Journal: Nature Communications

Article Title: Tumor suppressor p53 regulates intestinal type 2 immunity

doi: 10.1038/s41467-021-23587-x

Figure Lengend Snippet: a and b Lrmp interacted with IP3R proteins, including ITPR2, as determined by co-IP followed by LC-MS/MS assays in MEFs. a Lrmp-interacting proteins (label with red) mapped in “Chemo-sensing pathway” in KEGG. b Counts of Lrmp and IP3Rs detected by co-IP followed by LC-MS/MS assays in MEFs. c Lrmp-ITPR2 interaction was confirmed by co-IP assays followed by Western-blot assays in MEFs transfected with vectors expressing Lrmp-Flag and ITPR2-HA, respectively. d The binding region of Lrmp for ITPR2 was determined by co-IP followed by Western-blot assays in H1299 cells transfected with vectors expressing different Lrmp-Flag fragments and ITPR2-HA, respectively. Upper panel: schematic representation of vectors expressing full-length (FL) or serial deletion mutants of Lrmp-Flag. e The Lrmp-IPTR2 interaction was examined by PLA assays in WT and Lrmp−/− small intestinal tissues. Red: PLA signals of the Lrmp-ITPR2 interaction; green: Dclk1; DAPI: nucleus. f – h Ca 2+ flux in response to Ionomycin (2 µM) treatment in WT, p53−/− and Lrmp−/− MEFs with or without transfection of either FL- or ΔM-Lrmp-Flag vectors. f Average traces of Ca 2+ responses. g Relative Fluo-4 fluorescence obtained from Ca 2+ transients at peak (48 s). h Representative fluorescence images. The Fluo-4 fluorescence intensity before Ionomycin treatment was calculated as 1. In f , each curve represents an average of at least 90 cells. Curves of each cell are shown in Fig S . *** p < 0.001; ** p < 0.01; NS: non-significant, two-tailed Student’s t -test.

Article Snippet: In brief, tissue sections were incubated with anti-Lrmp (Biorbyt, orb166443, 1:50 dilution) and anti-ITPR2 (Novus, NB100-2466, 1:50 dilution) antibodies together with Duolink secondary antibodies.

Techniques: Co-Immunoprecipitation Assay, Liquid Chromatography with Mass Spectroscopy, Western Blot, Transfection, Expressing, Binding Assay, Fluorescence, Two Tailed Test