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Oxford Instruments
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Geomagic Inc
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Carl Zeiss
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Palleon Pharmaceuticals
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Palleon Pharmaceuticals
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COMSOL Inc
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Mercury Computer Systems Inc
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Image Search Results
Journal: Journal of cell science
Article Title: Visualizing extravasation dynamics of metastatic tumor cells.
doi: 10.1242/jcs.069443
Figure Lengend Snippet: Fig. 2. Tumor cells arrest in small vessels then actively migrate along the luminal surface of the vascular endothelium. (A)MDAwt cell expressing CFP and migrating within the ISV lumen for the indicated times (supplementary material Movie 1). Right panel shows 3D isosurface rendering of the tumor cell body for each time point (supplementary material Movie 2). (B)MDAtwist cell expressing RFP and migrating in the lumen of an ISV for the indicated times (supplementary material Movie 4). Right panel shows 3D isosurface rendering of the tumor cell body for each time point (supplementary material Movie 5). (C)Multi-color confocal images of MDAwt and MDA1KO cells labeled with CFP or RFP that have arrested in the ISV lumen. Lower panel shows a control 10m fluorescent Sepharose bead (yellow) arrested in the ISV. (D)Quantification of distance from tumor cell to vessel wall for MDAwt, MDA1KO cells, and Sepharose beads as shown in representative Fig. 2C above. Results are means ± s.e.m. Scale bars: 20m.
Article Snippet: Imaris Contour Surface function was used to manually isolate migrating tumor cell body which was then 3D rendered for each time point using
Techniques: Expressing, Labeling, Control