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Image Search Results
Journal: Stem Cells Translational Medicine
Article Title: A Proinflammatory Secretome Mediates the Impaired Immunopotency of Human Mesenchymal Stromal Cells in Elderly Patients with Atherosclerosis
doi: 10.1002/sctm.16-0221
Figure Lengend Snippet: Elderly MSCs secrete higher levels of senescence associated cytokines. (A) : Baseline production of cytokines and chemokines by MSCs from adult and elderly individuals assessed by V‐Plex assay. Data is reported as a ratio of secretion compared to the average of the A‐MSCs groups. The color scale represents fold change ( n = 5). (B–E) : Senescent associated cytokines and chemokines were confirmed by enzyme‐linked immunosorbent assays. IL‐6, IL‐8/CXCL8 (**, p < .01; n = 11), MCP‐1/CCL2 (***, p < .001, n = 11), MIF (*, p = .01 n = 6). Abbreviations: A‐MSCs, adult MSCs; E‐MSCs, elderly MSCs; MCP‐1, monocyte chemoattractant protein‐1; MIF, macrophage migration inhibitory factor.
Article Snippet: To evaluate the functional implications of IL‐6, IL‐8/CXCL8, MCP‐1/CCL2, and MIF as mediators of the MSCs:CD4 + T‐cell suppression, neutralization assays were performed by adding anti‐IL‐6 (20 μg/ml) (Abcam, Toronto, ON, Canada), anti‐IL‐8/CXCL8 (10 μg/ml), anti‐ MCP‐1/CCL2 (Abcam) (45 μg/ml) monoclonal antibodies or a
Techniques: Plex Assay, Migration