Journal: Cell reports

Article Title: Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4 + T cell subsets in vivo

doi: 10.1016/j.celrep.2021.109038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Iridium Interchelator Solution , Fluidigm , Cat#201192B.

Techniques: Virus, Recombinant, Electron Microscopy, Clinical Proteomics, Staining, Antibody Labeling, Cell Isolation, Software

Journal: Genetic Vaccines and Therapy

Article Title: Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells

doi: 10.1186/1479-0556-5-11

Figure Lengend Snippet: Effect of microtubule disrupting agent, nocodazole, on microtubule morphology and PEI polyplex trafficking . Untreated (A) and nocodazole treated cells (D) displaying normal and disrupted microtubules, highlighted by anti-β-tubulin visualized using FITC secondary antibody. Nucleus stained with DAPI (B, E) and merged immunofluorescence displayed as (C, F). Distribution of PEI/pDNA-YOYO in untreated (G) and nocodazole treated (J) cells with MitoTracker staining (H, K). Merge MitoTracker and PEI/pDNA-YOYO in untreated (I) and nocodazole treated (L) cells. Scale bar = 10 μm. Images represent typical result of two individual slides viewed for each sample, analyzed 5 h post transfection.

Article Snippet: Plasmid pCH110 was fluorescently labelled with the intercalating nucleic acid dye YOYO-1 iodide (diluted from 1 mM stock solution in DMSO; Molecular Probes).

Techniques: Staining, Immunofluorescence, Transfection