Journal: Scientific Reports

Article Title: Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder

doi: 10.1038/s41598-019-50388-6

Figure Lengend Snippet: Transcriptome analysis of passage 2 (P2) dUCs. Total RNA was extracted from P2 dUCs (n = 3), aHDFs (n = 3) and HUCs (n = 1) and subjected to RNA-seq analysis using Illumina Novaseq. 6000 (Illumina). ( a ) Hierarchical clustering sample tree analysis showing top 75% genes with maximum expression levels. ( b ) Heatmap of hierarchical clustering analysis showing the most variable top 50 genes. ( c ) Heatmaps for GO genesets comparing P2 dUCs with aHDFs are shown. ( d ) Volcano plot of differential gene expression analysis showing comparison between dUCs and aHDFs. Some genes specific to urothelial cells and fibroblasts were indicated by the red and blue arrows, respectively. ( e ) The KEGG pathway diagram comparing dUCs and aHDFs. Red color shows the genes highly expressed in dUCs, while green color shows genes highly expressed in aHDFs.

Article Snippet: Total RNA of human small intestine from five Caucasians aged 20 to 61 (#636539, Lot 1611206 A) and of human liver from four Asian and Caucasian aged 26 to 78 (#636531, Lot 1703002) was purchased from Clontech Laboratories.

Techniques: RNA Sequencing Assay, Expressing

Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine increases the mucin 5AC (MUC5AC) level and inhibits the expression of caudal type homeobox 2, MUC2, and Trefoil factor family 3 in Atp4a -/- mice. A: Expression of caudal type homeobox 2 (CDX2), mucin 2 (MUC2), Trefoil factor family 3 (TFF3) and MUC5AC in Atp4a -/- mouse gastric tissues was assessed via immunohistochemistry (IHC) staining ( n = 6); B: IHC scores of CDX2, MUC2, and TFF3 ( n = 6); C: IHC score of MUC5AC ( n = 6). a P < 0.01 vs Atp4a -/- ; b P < 0.01 vs wild-type (WT).

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Immunohistochemistry

Journal: World Journal of Gastrointestinal Oncology

Article Title: Taurine suppresses gastric intestinal metaplasia in patient-derived organoids and Atp4a -/- mice

doi: 10.4251/wjgo.v18.i2.114161

Figure Lengend Snippet: Taurine downregulates the protein expression of caudal type homeobox 2 and Trefoil factor family 3 in gastric intestinal metaplasia control organoids. A-C: Western blotting was conducted to assess the protein levels of caudal type homeobox 2 (CDX2) and Trefoil factor family 3 (TFF3); D-I: Relative protein levels were quantified. a P < 0.01 vs normal control; b P < 0.05 vs gastric intestinal metaplasia (GIM) control (CON); c P < 0.01 vs GIM CON.

Article Snippet: The gastric tissue sections were dewaxed, rehydrated, and incubated with primary antibodies against MUC5AC (1:200, GTX11335; GeneTex, Irvine, CA, United States), CDX2 (1:100, ab101532; Abcam, Cambridge, United Kingdom), MUC2 (1:2000, 27675-1-AP; Proteintech, Wuhan, China), and TFF3 (1:800, 23277-1-AP; Proteintech).

Techniques: Expressing, Control, Western Blot