Journal: World Journal of Stem Cells

Article Title: Intratracheal administration of umbilical cord-derived mesenchymal stem cells attenuates hyperoxia-induced multi-organ injury via heme oxygenase-1 and JAK/STAT pathways

doi: 10.4252/wjsc.v14.i7.556

Figure Lengend Snippet: Human umbilical cord-derived mesenchymal stem cells treatment modulates the hyperoxia-induced lung inflammation and oxidative stress. A: Statistical analyses of overall protein concentration in bronchoalveolar lavage fluid in the four groups ( n = 7); B-F: Statistical analyses of myeloperoxidase, tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6, and IL-10 levels in the lung tissues in the indicated groups ( n = 7); G and H: Macrophage inflammatory protein (MIP)-1α and MIP-1β mRNA expression in the indicated groups ( n = 5); I: Malondialdehyde levels were measured to evaluate the degree of oxidative reaction in the lung tissues ( n = 7). a P < 0.05; b P < 0.01; c P < 0.001. iT: Intratracheal; iP: Intraperitoneal; BALF: Bronchoalveolar lavage fluid; MPO: Myeloperoxidase; TNF-α: Tumor necrosis factor-alpha; IL: Interleukin; MIP: Macrophage inflammatory protein; MDA: Malondialdehyde; MSC: Mesenchymal stem cell.

Article Snippet: The total protein content of lung tissue homogenate was measured and samples were analyzed using the rat interleukin (IL)-1β (KE20005, Proteintech), IL-10 (KE20003, Proteintech), tumor necrosis factor-alpha (TNF-α) (KE20001, Proteintech), and IL-6 (SEKR-0005, Solarbio, China) enzyme-linked immunosorbent assay (ELISA) kits to determine the level of inflammatory cytokines in accordance with the manufacturer’s instructions.

Techniques: Derivative Assay, Protein Concentration, Expressing

Journal: World Journal of Stem Cells

Article Title: Intratracheal administration of umbilical cord-derived mesenchymal stem cells attenuates hyperoxia-induced multi-organ injury via heme oxygenase-1 and JAK/STAT pathways

doi: 10.4252/wjsc.v14.i7.556

Figure Lengend Snippet: Proposed mechanism of therapeutic effects of human umbilical cord-derived mesenchymal stem cells in hyperoxia-induced multiple organ injury. The administration of human umbilical cord-derived mesenchymal stem cells at postnatal day ameliorates hyperoxia-induced lung, heart, and kidney development, and reduces inflammatory and oxidative responses by activating heme oxygenase-1 expression and the JAK/STAT3 pathway. O 2 : Oxygen; hUC-MSCs: Human umbilical cord-derived mesenchymal stem cells; HO-1: Heme oxygenase-1; MPO: Myeloperoxidase; TNF-α: Tumor necrosis factor-alpha; IL: Interleukin; MDA: Malondialdehyde.

Article Snippet: The total protein content of lung tissue homogenate was measured and samples were analyzed using the rat interleukin (IL)-1β (KE20005, Proteintech), IL-10 (KE20003, Proteintech), tumor necrosis factor-alpha (TNF-α) (KE20001, Proteintech), and IL-6 (SEKR-0005, Solarbio, China) enzyme-linked immunosorbent assay (ELISA) kits to determine the level of inflammatory cytokines in accordance with the manufacturer’s instructions.

Techniques: Derivative Assay, Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Novel Nrf2 Activator Omaveloxolone Regulates Microglia Phenotype and Ameliorates Secondary Brain Injury after Intracerebral Hemorrhage in Mice

doi: 10.1155/2022/4564471

Figure Lengend Snippet: Omav reduced the expression of inflammatory cytokines but enhanced BV2 phagocytosis under the stress of OxyHb. (a–c) Levels of the Il-1β, Tnf-α , and Il-10 mRNAs in treated BV2 cells ( n = 3). (d) Representative fluorescent images of the phagocytosis assay in BV2 cells. β -Actin in BV2 cells was labeled with phalloidin-FITC, and RBCs were labeled with DiI. Scale bar: 50 μ m. (e) Quantification of the phagocyte proportion according to fluorescence ( n = 6). (f and g) The concentrations of IL-1 β and TNF- α were detected using ELISAs ( n = 6). (h) Phagocytosis ratio of BV2 cells in each group detected via FCM. (i) Quantification of the ratio of BV2 cells phagocytoses labeled RBCs from each group ( n = 3). Data are presented as the means ± SEM. ∗ P < 0.05 and ∗∗ P < 0.01 compared with the control group; # P < 0.05 and ## P < 0.01 compared with the OxyHb group; † P < 0.05 and †† P < 0.01 compared with the OxyHb+Omav group.

Article Snippet: The levels of TNF- α (cat. no. KE10003) and IL-1 β (cat. no. KE10003) in the supernatants of treated BV2 cells were examined using ELISA kits (Proteintech) according to the manufacturer's instructions.

Techniques: Expressing, Phagocytosis Assay, Labeling, Fluorescence, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Novel Nrf2 Activator Omaveloxolone Regulates Microglia Phenotype and Ameliorates Secondary Brain Injury after Intracerebral Hemorrhage in Mice

doi: 10.1155/2022/4564471

Figure Lengend Snippet: Omav reduced the expression of proinflammatory genes and proteins after ICH. (a) Immunofluorescence staining for Iba-1 (green) and iNOS (red) in the ipsilateral basal ganglia region 3 days after ICH; the nuclei were stained with DAPI (blue); scale bar: 50 μ m ( n = 6). (b) Quantification of the ratio of microglia expressing iNOS. (c–e) qRT-PCR analysis of the expression of the mRNAs encoding iNOS, IL-1 β , and TNF- α ( n = 3). (f) Western blot showing iNOS levels in peripheral hematoma tissues from each group ( n = 6). (g) Quantitative analysis of the iNOS band. (h–i) The concentrations of IL-1 β and TNF- α in perihematoma 3 days after ICH were detected using ELISAs ( n = 6). Data are presented as the means ± SEM. ∗ P < 0.05 and ∗∗ P < 0.01 compared with the control group; # P < 0.05 and ## P < 0.01 compared with the OxyHb group; † P < 0.05 and †† P < 0.01 compared with the OxyHb+Omav group.

Article Snippet: The levels of TNF- α (cat. no. KE10003) and IL-1 β (cat. no. KE10003) in the supernatants of treated BV2 cells were examined using ELISA kits (Proteintech) according to the manufacturer's instructions.

Techniques: Expressing, Immunofluorescence, Staining, Quantitative RT-PCR, Western Blot, Control