interleukin 10 Search Results


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    Thermo Fisher il 10
    <t>IL-10</t> levels in the supernatant of BMDMs under different hydrostatic pressures. Graphs displaying the calculated IL-10 levels in the supernatant from BMDMs on day 2 (A) and day 7 (B) ( n = 6) as measured by ELISA. Statistical significance between different pressures: * p
    Il 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 10/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 10 - by Bioz Stars, 2021-06
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    86
    R&D Systems il 10
    Survival and inflammation profile of <t>IL-10-deficient</t> mice inoculated with T. brucei infected tsetse fly. (A) Survival of WT and IL-10-deficient mice after T. brucei infection via tsetse fly, where the median survival (MS) of each group is indicated in days. Data are represented as mean of at least 6 mice per group ± SEM and are representative of 2 independent experiments. (B) Cytokine levels were measured by ELISA in plasma of infected WT and IL-10-deficient mice at 6 days p.i. Data are represented as mean of at least four mice per group ± SEM, where * p ≤ 0.05, *** p ≤ 0.001, and are representative of three independent experiments. The dashed line represents the detection limit.
    Il 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 10/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 10 - by Bioz Stars, 2021-06
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    86
    BioLegend il 10
    Eomes promotes the expression of <t>IL-10</t> in activated CD8 + T cells. (A) CD8 + T cells isolated from WT, TKO and EKO mice were cultured under Tc0 conditions for 5 days. On day 5, cells were restimulated (bottom panel) with PMA and ionomycin and analyzed for IFNÉ£ and IL-10 expression. Cells are gated on live/CD8 + cells. (B) Representative percentages of IL-10-secreting CD8 + T cells in WT and EKO mice from (A). Plots are representative of 5 independent experiments (n=5). (C) WT or EKO CD8 + T cells were activated in vitro and transduced with Eomes or empty-vector retrovirus. On day 5, RNA was harvested from cell cultures and transcript levels were analyzed by RT-qPCR. Expression levels were analyzed relative to empty-vector transduced WT cells and normalized to GAPDH. Error bars represent standard deviation of replicates done in triplicate. Asterisks indicate a p-value
    Il 10, supplied by BioLegend, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 10/product/BioLegend
    Average 86 stars, based on 1 article reviews
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    il 10 - by Bioz Stars, 2021-06
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    86
    PeproTech il 10
    ER stress can be suppressed by B. dentium , γ-glutamylcysteine, and <t>IL-10.</t> a . qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor thapsigargin. Cells were treated with either media, 50% un-inoculated LDM4 (LDM4), 50% B. dentium LDM4 (Bd), 2 mM γ-glutamylcysteine (yGC), or 100 ng/mL IL-10 (IL-10) (n = 6/experiment). b . qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor tunicamycin (n = 6/experiment). c . Propidium iodide staining of T84 cells after 48 hr incubation with ER stressors (thapsigargin or tunicamycin) or oxidative stressor hydrogen peroxide (H 2 0 2 ) (n = 6/experiment). *p
    Il 10, supplied by PeproTech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 10/product/PeproTech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 10 - by Bioz Stars, 2021-06
    86/100 stars
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    Category Kits CLIA Kits Human IL 10 Interleukin 10 CLIA Kit Size 96T Price 682 Reactivity Human
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    Category Kits CLIA Kits Mouse IL 10 Interleukin 10 CLIA Kit Size 96T Price 682 Reactivity Mouse
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    Image Search Results


    IL-10 levels in the supernatant of BMDMs under different hydrostatic pressures. Graphs displaying the calculated IL-10 levels in the supernatant from BMDMs on day 2 (A) and day 7 (B) ( n = 6) as measured by ELISA. Statistical significance between different pressures: * p

    Journal: Frontiers in Immunology

    Article Title: Increased Hydrostatic Pressure Promotes Primary M1 Reaction and Secondary M2 Polarization in Macrophages

    doi: 10.3389/fimmu.2020.573955

    Figure Lengend Snippet: IL-10 levels in the supernatant of BMDMs under different hydrostatic pressures. Graphs displaying the calculated IL-10 levels in the supernatant from BMDMs on day 2 (A) and day 7 (B) ( n = 6) as measured by ELISA. Statistical significance between different pressures: * p

    Article Snippet: AntibodiesAntibodies for immunofluorescence were purchased from the following sources: F4/80 (0.2 μg/ml, BM8, OriGene, Rockville, MD, USA), TNF-α (2.5 μg/ml, MP6-XT3, eBioscience, San Diego, CA, USA), IL-10 (2.5 μg/ml, JES5-2A5, eBioscience), fibronectin (0.7 μg/ml, Polyclonal, invitrogen, Carlsbad, CA, USA), and collagen IV (2.5 μg/ml, Polyclonal, Invitrogen).

    Techniques: Enzyme-linked Immunosorbent Assay

    Frequency of IL-10 + /F4/80 + events under increased HP. To calculate differences in the IL-10 + /F4/80 + populations, BMDMs were stained with antibodies targeting F4/80 and IL-10. The specimens were then analyzed by flow cytometry. (A) Dotplots showing isotype control and positive staining of IL-10 in cells. Graphs showing the calculated frequency of IL-10 + /F4/80 + events on day 2 (B) and day 7 (C) ( n = 3). Statistical significance between different pressures: * p

    Journal: Frontiers in Immunology

    Article Title: Increased Hydrostatic Pressure Promotes Primary M1 Reaction and Secondary M2 Polarization in Macrophages

    doi: 10.3389/fimmu.2020.573955

    Figure Lengend Snippet: Frequency of IL-10 + /F4/80 + events under increased HP. To calculate differences in the IL-10 + /F4/80 + populations, BMDMs were stained with antibodies targeting F4/80 and IL-10. The specimens were then analyzed by flow cytometry. (A) Dotplots showing isotype control and positive staining of IL-10 in cells. Graphs showing the calculated frequency of IL-10 + /F4/80 + events on day 2 (B) and day 7 (C) ( n = 3). Statistical significance between different pressures: * p

    Article Snippet: AntibodiesAntibodies for immunofluorescence were purchased from the following sources: F4/80 (0.2 μg/ml, BM8, OriGene, Rockville, MD, USA), TNF-α (2.5 μg/ml, MP6-XT3, eBioscience, San Diego, CA, USA), IL-10 (2.5 μg/ml, JES5-2A5, eBioscience), fibronectin (0.7 μg/ml, Polyclonal, invitrogen, Carlsbad, CA, USA), and collagen IV (2.5 μg/ml, Polyclonal, Invitrogen).

    Techniques: Staining, Flow Cytometry

    IL-10 expression in BMDMs under different hydrostatic pressures. (A) Representative immunofluorescence images of IL-10 (red) staining in BMDMs under different hydrostatic pressures. F4/80-positive (green) cells represent macrophages. Scale bar = 50 μm. Graphs displaying the calculated MFI/cell of IL-10 on day 2 (B) and day 7 (C) ( n = 9). Statistical significance between different pressures: * p

    Journal: Frontiers in Immunology

    Article Title: Increased Hydrostatic Pressure Promotes Primary M1 Reaction and Secondary M2 Polarization in Macrophages

    doi: 10.3389/fimmu.2020.573955

    Figure Lengend Snippet: IL-10 expression in BMDMs under different hydrostatic pressures. (A) Representative immunofluorescence images of IL-10 (red) staining in BMDMs under different hydrostatic pressures. F4/80-positive (green) cells represent macrophages. Scale bar = 50 μm. Graphs displaying the calculated MFI/cell of IL-10 on day 2 (B) and day 7 (C) ( n = 9). Statistical significance between different pressures: * p

    Article Snippet: AntibodiesAntibodies for immunofluorescence were purchased from the following sources: F4/80 (0.2 μg/ml, BM8, OriGene, Rockville, MD, USA), TNF-α (2.5 μg/ml, MP6-XT3, eBioscience, San Diego, CA, USA), IL-10 (2.5 μg/ml, JES5-2A5, eBioscience), fibronectin (0.7 μg/ml, Polyclonal, invitrogen, Carlsbad, CA, USA), and collagen IV (2.5 μg/ml, Polyclonal, Invitrogen).

    Techniques: Expressing, Immunofluorescence, Staining

    Survival and inflammation profile of IL-10-deficient mice inoculated with T. brucei infected tsetse fly. (A) Survival of WT and IL-10-deficient mice after T. brucei infection via tsetse fly, where the median survival (MS) of each group is indicated in days. Data are represented as mean of at least 6 mice per group ± SEM and are representative of 2 independent experiments. (B) Cytokine levels were measured by ELISA in plasma of infected WT and IL-10-deficient mice at 6 days p.i. Data are represented as mean of at least four mice per group ± SEM, where * p ≤ 0.05, *** p ≤ 0.001, and are representative of three independent experiments. The dashed line represents the detection limit.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: Survival and inflammation profile of IL-10-deficient mice inoculated with T. brucei infected tsetse fly. (A) Survival of WT and IL-10-deficient mice after T. brucei infection via tsetse fly, where the median survival (MS) of each group is indicated in days. Data are represented as mean of at least 6 mice per group ± SEM and are representative of 2 independent experiments. (B) Cytokine levels were measured by ELISA in plasma of infected WT and IL-10-deficient mice at 6 days p.i. Data are represented as mean of at least four mice per group ± SEM, where * p ≤ 0.05, *** p ≤ 0.001, and are representative of three independent experiments. The dashed line represents the detection limit.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Mouse Assay, Infection, Enzyme-linked Immunosorbent Assay

    IL-27-independent IL-10 production following T. brucei infection. (A) Splenic percentage of IL-10eGFP + as well as (B) IL-10 levels in supernatant of ex vivo spleen cell cultures and in serum were assessed in untreated and anti-IL-27-treated infected Vert-X mice. IL-10 levels were measured by ELISA in plasma of infected WT and anti-IL-27-treated WT at day 6, 9, and 12 p.i. (C) . Data are represented as mean of minimum 4 mice per group ± SEM, where *** p ≤ 0.001, and are representative of at least TWO independent experiments.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: IL-27-independent IL-10 production following T. brucei infection. (A) Splenic percentage of IL-10eGFP + as well as (B) IL-10 levels in supernatant of ex vivo spleen cell cultures and in serum were assessed in untreated and anti-IL-27-treated infected Vert-X mice. IL-10 levels were measured by ELISA in plasma of infected WT and anti-IL-27-treated WT at day 6, 9, and 12 p.i. (C) . Data are represented as mean of minimum 4 mice per group ± SEM, where *** p ≤ 0.001, and are representative of at least TWO independent experiments.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Infection, Ex Vivo, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Cellular source of IL-10 production following T. brucei infection. IL-10 levels were measured by ELISA in plasma of infected WT and anti-NK-, anti-CD8- and CD4-treated WT at day 6 and 9 p.i. (A) as well as in WT and anti-CD8- or CD4-treated or anti-NK1.1-treated WT mice (B) and in WT and anti-CD8- and CD4-treated 9 days p.i. (C) . (D) At day 8 p.i., CD11a and CD49d expression on splenic CD8 + and CD4 + T cells was analyzed. Both CD11a + CD49d + and CD11a − CD49d − CD8 + and CD4 + T cell subsets were analyzed for the expression of IL-10eGFP and intracellular expression of IFNg. Data are represented as mean of at least four mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, and are representative of two independent experiments.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: Cellular source of IL-10 production following T. brucei infection. IL-10 levels were measured by ELISA in plasma of infected WT and anti-NK-, anti-CD8- and CD4-treated WT at day 6 and 9 p.i. (A) as well as in WT and anti-CD8- or CD4-treated or anti-NK1.1-treated WT mice (B) and in WT and anti-CD8- and CD4-treated 9 days p.i. (C) . (D) At day 8 p.i., CD11a and CD49d expression on splenic CD8 + and CD4 + T cells was analyzed. Both CD11a + CD49d + and CD11a − CD49d − CD8 + and CD4 + T cell subsets were analyzed for the expression of IL-10eGFP and intracellular expression of IFNg. Data are represented as mean of at least four mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, and are representative of two independent experiments.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Mouse Assay, Expressing

    T cell-derived Blimp-1 signaling pathway is required to dampen inflammation following T. brucei infection. (A) Percentage of splenic IL-10 + CD8 + and CD4 + T cell subsets was analyzed at day 9 p.i. both in WT and T cell-specific Prdm1-deficient (Prdm1 CKO) mice following T. brucei infection. (B) The CD11a and CD49d expression on splenic CD8 + and CD4 + T cells were analyzed at day 12 p.i. in WT and T cell-specific Prdm1-deficient (Prdm1 CKO) mice. Data are represented as mean of at least 3 mice per group ± SEM and are representative of 3 independent experiments. (C) Cytokine levels in serum at day 12 p.i. and (D) survival of WT and Prdm1 CKO mice were monitored following T. brucei infection, where the median survival (MS) of each group is indicated in days. Data are represented as mean of at least four mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, and are representative of two independent experiments.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: T cell-derived Blimp-1 signaling pathway is required to dampen inflammation following T. brucei infection. (A) Percentage of splenic IL-10 + CD8 + and CD4 + T cell subsets was analyzed at day 9 p.i. both in WT and T cell-specific Prdm1-deficient (Prdm1 CKO) mice following T. brucei infection. (B) The CD11a and CD49d expression on splenic CD8 + and CD4 + T cells were analyzed at day 12 p.i. in WT and T cell-specific Prdm1-deficient (Prdm1 CKO) mice. Data are represented as mean of at least 3 mice per group ± SEM and are representative of 3 independent experiments. (C) Cytokine levels in serum at day 12 p.i. and (D) survival of WT and Prdm1 CKO mice were monitored following T. brucei infection, where the median survival (MS) of each group is indicated in days. Data are represented as mean of at least four mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, and are representative of two independent experiments.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Derivative Assay, Infection, Mouse Assay, Expressing

    IL-10 production in mouse spleen and liver cell cultures following T. brucei infection. Serum and supernatants from ex vivo cultured total spleen cells, purified leukocyte liver cells from naïve, day 6 and day 8 p.i and pooled peripheral lymph node (pLN) cells from naïve and day 8 p.i. were analyzed by ELISA for IL-10 production. Data are represented as mean of at least three mice per group ± SEM, where * p ≤ 0.05, and are representative of three independent experiments. The dashed line represents the detection limit.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: IL-10 production in mouse spleen and liver cell cultures following T. brucei infection. Serum and supernatants from ex vivo cultured total spleen cells, purified leukocyte liver cells from naïve, day 6 and day 8 p.i and pooled peripheral lymph node (pLN) cells from naïve and day 8 p.i. were analyzed by ELISA for IL-10 production. Data are represented as mean of at least three mice per group ± SEM, where * p ≤ 0.05, and are representative of three independent experiments. The dashed line represents the detection limit.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Infection, Ex Vivo, Cell Culture, Purification, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Hematopoietic cells constitute a major source of IL-10 production during T. brucei infection. (A) Survival of the chimeric mice 8 weeks post-transplantation as well as IL-10-deficient mice following T. brucei infection i.p., where the median survival (MS) of each group is indicated in days. (B) Cytokine levels were measured by ELISA in plasma of infected chimeric and IL-10-deficient mice 8 days p.i. Data are represented as mean of at least 4 mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and are representative of 2 independent experiments. The dashed line represents the detection limit.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: Hematopoietic cells constitute a major source of IL-10 production during T. brucei infection. (A) Survival of the chimeric mice 8 weeks post-transplantation as well as IL-10-deficient mice following T. brucei infection i.p., where the median survival (MS) of each group is indicated in days. (B) Cytokine levels were measured by ELISA in plasma of infected chimeric and IL-10-deficient mice 8 days p.i. Data are represented as mean of at least 4 mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and are representative of 2 independent experiments. The dashed line represents the detection limit.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Infection, Mouse Assay, Transplantation Assay, Enzyme-linked Immunosorbent Assay

    Cellular characterization of IL-10 producing cells in spleen and liver following T. brucei infection. (A) Percentages and (B) total cell number of IL-10 producing cells in infected liver (LI inf) and spleen (SP inf) at day 3, 6, and 8 p.i. (C) Relative contribution of the different cellular sources producing IL-10. The surface of the diagram is representative of the total number of IL-10 + cells present in liver and spleen at day 6 and day 8 p.i., which is described in (B) . (D) Histogram showing the intensity of IL-10-eGFP signal as well as the percentage and MFI of IL-10 + cells in different cell subsets. The red dotted line is giving an indication of the relative IL-10-eGFP signal differences between the different cell subsets, whereas the gray histogram represent the relative IL-10-eGFP signal of these different cell subsets in infected non-reporter mice. Data are represented as mean of at least three mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and are representative of 3 independent experiments.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: Cellular characterization of IL-10 producing cells in spleen and liver following T. brucei infection. (A) Percentages and (B) total cell number of IL-10 producing cells in infected liver (LI inf) and spleen (SP inf) at day 3, 6, and 8 p.i. (C) Relative contribution of the different cellular sources producing IL-10. The surface of the diagram is representative of the total number of IL-10 + cells present in liver and spleen at day 6 and day 8 p.i., which is described in (B) . (D) Histogram showing the intensity of IL-10-eGFP signal as well as the percentage and MFI of IL-10 + cells in different cell subsets. The red dotted line is giving an indication of the relative IL-10-eGFP signal differences between the different cell subsets, whereas the gray histogram represent the relative IL-10-eGFP signal of these different cell subsets in infected non-reporter mice. Data are represented as mean of at least three mice per group ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and are representative of 3 independent experiments.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Infection, Mouse Assay

    Cellular characterization of IL-10 producing cells in spleen and liver following T. brucei infection by tsetse fly. (A) Percentages and (B) total cell number of IL-10 producing cells in infected liver (LI inf) and spleen (SP inf) at day 4 and 7 p.i. (C) Relative contribution of the different cellular sources producing IL-10. The surface of the diagram is representative of the total number of IL-10 + cells present in liver and spleen at day 4 and day 7 p.i., which is described in (B) . Data are represented as mean of at least three mice per group ± SEM, where *** p ≤ 0.001, and are representative of three independent experiments.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: Cellular characterization of IL-10 producing cells in spleen and liver following T. brucei infection by tsetse fly. (A) Percentages and (B) total cell number of IL-10 producing cells in infected liver (LI inf) and spleen (SP inf) at day 4 and 7 p.i. (C) Relative contribution of the different cellular sources producing IL-10. The surface of the diagram is representative of the total number of IL-10 + cells present in liver and spleen at day 4 and day 7 p.i., which is described in (B) . Data are represented as mean of at least three mice per group ± SEM, where *** p ≤ 0.001, and are representative of three independent experiments.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Infection, Mouse Assay

    Uncontrolled inflammation in T. brucei -infected IL-10-deficient mice. (A–C) Cytokine levels were measured by ELISA in plasma of naive mice, infected WT mice on days 5, 6, 7, 8, and 10 p.i. and naive vs. infected IL-10-deficient mice on day 7 p.i. Data are represented as mean of 6 mice per group ± SEM and are representative of 2 independent experiments. (D) Survival of WT and IL-10-deficient mice following T. brucei infection i.p., where the median survival (MS) of each group is indicated in days. Data are presented as a pool of two representative independent experiments with a minimum of three mice per group, where *** p ≤ 0.001.

    Journal: Frontiers in Immunology

    Article Title: A Critical Blimp-1-Dependent IL-10 Regulatory Pathway in T Cells Protects From a Lethal Pro-inflammatory Cytokine Storm During Acute Experimental Trypanosoma brucei Infection

    doi: 10.3389/fimmu.2020.01085

    Figure Lengend Snippet: Uncontrolled inflammation in T. brucei -infected IL-10-deficient mice. (A–C) Cytokine levels were measured by ELISA in plasma of naive mice, infected WT mice on days 5, 6, 7, 8, and 10 p.i. and naive vs. infected IL-10-deficient mice on day 7 p.i. Data are represented as mean of 6 mice per group ± SEM and are representative of 2 independent experiments. (D) Survival of WT and IL-10-deficient mice following T. brucei infection i.p., where the median survival (MS) of each group is indicated in days. Data are presented as a pool of two representative independent experiments with a minimum of three mice per group, where *** p ≤ 0.001.

    Article Snippet: Alternatively, culture medium and serum concentrations of IL-6, TNF, IFN-γ, and IL-10 (R & D Systems) were determined by ELISA as recommended by the suppliers.

    Techniques: Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Eomes promotes the expression of IL-10 in activated CD8 + T cells. (A) CD8 + T cells isolated from WT, TKO and EKO mice were cultured under Tc0 conditions for 5 days. On day 5, cells were restimulated (bottom panel) with PMA and ionomycin and analyzed for IFNÉ£ and IL-10 expression. Cells are gated on live/CD8 + cells. (B) Representative percentages of IL-10-secreting CD8 + T cells in WT and EKO mice from (A). Plots are representative of 5 independent experiments (n=5). (C) WT or EKO CD8 + T cells were activated in vitro and transduced with Eomes or empty-vector retrovirus. On day 5, RNA was harvested from cell cultures and transcript levels were analyzed by RT-qPCR. Expression levels were analyzed relative to empty-vector transduced WT cells and normalized to GAPDH. Error bars represent standard deviation of replicates done in triplicate. Asterisks indicate a p-value

    Journal: Cellular immunology

    Article Title: Eomesodermin driven IL-10 production in effector CD8+ T cells promotes a memory phenotype.

    doi: 10.1016/j.cellimm.2018.11.008

    Figure Lengend Snippet: Eomes promotes the expression of IL-10 in activated CD8 + T cells. (A) CD8 + T cells isolated from WT, TKO and EKO mice were cultured under Tc0 conditions for 5 days. On day 5, cells were restimulated (bottom panel) with PMA and ionomycin and analyzed for IFNÉ£ and IL-10 expression. Cells are gated on live/CD8 + cells. (B) Representative percentages of IL-10-secreting CD8 + T cells in WT and EKO mice from (A). Plots are representative of 5 independent experiments (n=5). (C) WT or EKO CD8 + T cells were activated in vitro and transduced with Eomes or empty-vector retrovirus. On day 5, RNA was harvested from cell cultures and transcript levels were analyzed by RT-qPCR. Expression levels were analyzed relative to empty-vector transduced WT cells and normalized to GAPDH. Error bars represent standard deviation of replicates done in triplicate. Asterisks indicate a p-value

    Article Snippet: ​ T-bet and Eomes regulate a unique gene signature in effector CD8+ T cells Eomes regulates CD62L expression specifically in central-memory T cells in vivo Eomes drives expression of both CD62L and IL-10 in effector CD8+ T cells In a feed-forward loop, IL-10 promotes CD62L expression in effector CD8+ T cells

    Techniques: Expressing, Isolation, Mouse Assay, Cell Culture, In Vitro, Transduction, Plasmid Preparation, Quantitative RT-PCR, Standard Deviation

    IL-10 maintains the expression of the T cm -associated molecule CD62L. (A) WT CD8 + T cells were cultured for 5 days under Tc0 conditions with or without the addition of exogenous IL-10 (20ng/ml). On day 5, cells were analyzed for expression of CD62L by FACS. (B) The ratio of CD62L expression in IL-10-treated vs untreated CD8+ T cells is shown. Data are representative of cell cultures from 7 mice (n=7) in 3 independent experiments. Median MFI values were used for analysis.

    Journal: Cellular immunology

    Article Title: Eomesodermin driven IL-10 production in effector CD8+ T cells promotes a memory phenotype.

    doi: 10.1016/j.cellimm.2018.11.008

    Figure Lengend Snippet: IL-10 maintains the expression of the T cm -associated molecule CD62L. (A) WT CD8 + T cells were cultured for 5 days under Tc0 conditions with or without the addition of exogenous IL-10 (20ng/ml). On day 5, cells were analyzed for expression of CD62L by FACS. (B) The ratio of CD62L expression in IL-10-treated vs untreated CD8+ T cells is shown. Data are representative of cell cultures from 7 mice (n=7) in 3 independent experiments. Median MFI values were used for analysis.

    Article Snippet: ​ T-bet and Eomes regulate a unique gene signature in effector CD8+ T cells Eomes regulates CD62L expression specifically in central-memory T cells in vivo Eomes drives expression of both CD62L and IL-10 in effector CD8+ T cells In a feed-forward loop, IL-10 promotes CD62L expression in effector CD8+ T cells

    Techniques: Expressing, Cell Culture, FACS, Mouse Assay

    Eomes does not require IL-10 to induce CD62L expression in CD8 + T cells. (A) CD8 + T cells were isolated from WT or IL-10KO mice and stimulated under Tc0 conditions for 2 days. On day 2, cells were transduced with Eomes-containing or empty-vector retrovirus (RV) and cultured through day 5. Cells were then analyzed for CD62L and Eomes expression by FACS. Cells are gated on live/CD8 + /Thy1.1 + populations. (B) Representative frequency of CD62L hi cells from (A). (C) Representative CD62L MFI values from CD62L + populations in (A). Plots are representative of 2 independent experiments with 2 mice per genotype (n=4).

    Journal: Cellular immunology

    Article Title: Eomesodermin driven IL-10 production in effector CD8+ T cells promotes a memory phenotype.

    doi: 10.1016/j.cellimm.2018.11.008

    Figure Lengend Snippet: Eomes does not require IL-10 to induce CD62L expression in CD8 + T cells. (A) CD8 + T cells were isolated from WT or IL-10KO mice and stimulated under Tc0 conditions for 2 days. On day 2, cells were transduced with Eomes-containing or empty-vector retrovirus (RV) and cultured through day 5. Cells were then analyzed for CD62L and Eomes expression by FACS. Cells are gated on live/CD8 + /Thy1.1 + populations. (B) Representative frequency of CD62L hi cells from (A). (C) Representative CD62L MFI values from CD62L + populations in (A). Plots are representative of 2 independent experiments with 2 mice per genotype (n=4).

    Article Snippet: ​ T-bet and Eomes regulate a unique gene signature in effector CD8+ T cells Eomes regulates CD62L expression specifically in central-memory T cells in vivo Eomes drives expression of both CD62L and IL-10 in effector CD8+ T cells In a feed-forward loop, IL-10 promotes CD62L expression in effector CD8+ T cells

    Techniques: Expressing, Isolation, Mouse Assay, Transduction, Plasmid Preparation, Cell Culture, FACS

    ER stress can be suppressed by B. dentium , γ-glutamylcysteine, and IL-10. a . qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor thapsigargin. Cells were treated with either media, 50% un-inoculated LDM4 (LDM4), 50% B. dentium LDM4 (Bd), 2 mM γ-glutamylcysteine (yGC), or 100 ng/mL IL-10 (IL-10) (n = 6/experiment). b . qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor tunicamycin (n = 6/experiment). c . Propidium iodide staining of T84 cells after 48 hr incubation with ER stressors (thapsigargin or tunicamycin) or oxidative stressor hydrogen peroxide (H 2 0 2 ) (n = 6/experiment). *p

    Journal: Gut Microbes

    Article Title: Bifidobacterium dentium-derived y-glutamylcysteine suppresses ER-mediated goblet cell stress and reduces TNBS-driven colonic inflammation

    doi: 10.1080/19490976.2021.1902717

    Figure Lengend Snippet: ER stress can be suppressed by B. dentium , γ-glutamylcysteine, and IL-10. a . qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor thapsigargin. Cells were treated with either media, 50% un-inoculated LDM4 (LDM4), 50% B. dentium LDM4 (Bd), 2 mM γ-glutamylcysteine (yGC), or 100 ng/mL IL-10 (IL-10) (n = 6/experiment). b . qPCR analysis of T84 monolayers after 6 hr incubation with or without the ER-stressor tunicamycin (n = 6/experiment). c . Propidium iodide staining of T84 cells after 48 hr incubation with ER stressors (thapsigargin or tunicamycin) or oxidative stressor hydrogen peroxide (H 2 0 2 ) (n = 6/experiment). *p

    Article Snippet: IL-10 is commonly produced by dendritic cells and can be produced in response to bacterial stimulation., To determine if B. dentium secreted factors could stimulate IL-10 from immune cells, we generated bone marrow-derived mouse dendritic cells.

    Techniques: Real-time Polymerase Chain Reaction, Incubation, Staining

    B. dentium and γ-glutamylcysteine promote the retention of colonic goblet cells and mucus. a . Representative images of PAS-AB stains of untreated control animals and TNBS treated animals receiving PBS vehicle, live B. dentium or γ-glutamylcysteine, and B. dentium mono-associated colon (20x, scale bar = 50 µm). b . Representative images of MUC2 and γ-actin of untreated control animals and TNBS-treated animals receiving PBS vehicle, live B. dentium or γ-glutamylcysteine, and B. dentium mono-associated colon (scale bar = 50 µm). c . Colonic mRNA expression of Muc2 in untreated control animals or TNBS-treated animals receiving PBS-vehicle, B. dentium (Bd) or γ-glutamylcysteine (yGC). d . Colonic mRNA expression of IL-10 in untreated control animals or TNBS-treated animals receiving PBS-vehicle, B. dentium (Bd) or γ-glutamylcysteine (yGC). n = 5 mice/group. *p

    Journal: Gut Microbes

    Article Title: Bifidobacterium dentium-derived y-glutamylcysteine suppresses ER-mediated goblet cell stress and reduces TNBS-driven colonic inflammation

    doi: 10.1080/19490976.2021.1902717

    Figure Lengend Snippet: B. dentium and γ-glutamylcysteine promote the retention of colonic goblet cells and mucus. a . Representative images of PAS-AB stains of untreated control animals and TNBS treated animals receiving PBS vehicle, live B. dentium or γ-glutamylcysteine, and B. dentium mono-associated colon (20x, scale bar = 50 µm). b . Representative images of MUC2 and γ-actin of untreated control animals and TNBS-treated animals receiving PBS vehicle, live B. dentium or γ-glutamylcysteine, and B. dentium mono-associated colon (scale bar = 50 µm). c . Colonic mRNA expression of Muc2 in untreated control animals or TNBS-treated animals receiving PBS-vehicle, B. dentium (Bd) or γ-glutamylcysteine (yGC). d . Colonic mRNA expression of IL-10 in untreated control animals or TNBS-treated animals receiving PBS-vehicle, B. dentium (Bd) or γ-glutamylcysteine (yGC). n = 5 mice/group. *p

    Article Snippet: IL-10 is commonly produced by dendritic cells and can be produced in response to bacterial stimulation., To determine if B. dentium secreted factors could stimulate IL-10 from immune cells, we generated bone marrow-derived mouse dendritic cells.

    Techniques: Expressing, Mouse Assay

    Dendritic cells secrete IL-10 in response to B. dentium -conditioned media.a Representative phase-contrast image of mouse bone-marrow derived dendritic cells and IL-10 measurements of dendritic cell supernatants by ELISA. Dendritic cells (100x, scale bar = 100 μm) were incubated with either media, 25% un-inoculated LDM4 media, 25% B. dentium conditioned LDM4 media or 2 mM γ-glutamylcysteine for 16 hr. b . Representative phase-contrast image of colonic organoid generated from germ-free mice and IL-10 measurements of organoid supernatant by ELISA. Colonic organoids (400x, scale bar = 50 μm) were incubated with either media, 25% uninoculated LDM4 media, 25% B. dentium- conditioned LDM4 media or 2 mM γ-glutamylcysteine for 16 hr. n = 3/experiments, repeated 2 independent times. *p

    Journal: Gut Microbes

    Article Title: Bifidobacterium dentium-derived y-glutamylcysteine suppresses ER-mediated goblet cell stress and reduces TNBS-driven colonic inflammation

    doi: 10.1080/19490976.2021.1902717

    Figure Lengend Snippet: Dendritic cells secrete IL-10 in response to B. dentium -conditioned media.a Representative phase-contrast image of mouse bone-marrow derived dendritic cells and IL-10 measurements of dendritic cell supernatants by ELISA. Dendritic cells (100x, scale bar = 100 μm) were incubated with either media, 25% un-inoculated LDM4 media, 25% B. dentium conditioned LDM4 media or 2 mM γ-glutamylcysteine for 16 hr. b . Representative phase-contrast image of colonic organoid generated from germ-free mice and IL-10 measurements of organoid supernatant by ELISA. Colonic organoids (400x, scale bar = 50 μm) were incubated with either media, 25% uninoculated LDM4 media, 25% B. dentium- conditioned LDM4 media or 2 mM γ-glutamylcysteine for 16 hr. n = 3/experiments, repeated 2 independent times. *p

    Article Snippet: IL-10 is commonly produced by dendritic cells and can be produced in response to bacterial stimulation., To determine if B. dentium secreted factors could stimulate IL-10 from immune cells, we generated bone marrow-derived mouse dendritic cells.

    Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Incubation, Generated, Mouse Assay

    B. dentium promotes colon MUC2 and IL-10 secretion in gnotobiotic mice. a . Representative images of H E and Periodic Acid Schiff-Alcian Blue (PAS-AB) stains of germ-free and B. dentium mono-associated colon (scale bar = 50 µm). b . Colonic mRNA expression of Muc2 . c . Colonic mRNA expression of IL-10 . d . Serum levels of IL-10 by ELISA. e-g . Colonic mRNA expression of ER-stress related genes (e) GRP-78 , (f) CHOP and (g) sXBP1 . All analyses were performed in germ free (n = 10) and B. dentium mono-associated mice (n = 10). *p

    Journal: Gut Microbes

    Article Title: Bifidobacterium dentium-derived y-glutamylcysteine suppresses ER-mediated goblet cell stress and reduces TNBS-driven colonic inflammation

    doi: 10.1080/19490976.2021.1902717

    Figure Lengend Snippet: B. dentium promotes colon MUC2 and IL-10 secretion in gnotobiotic mice. a . Representative images of H E and Periodic Acid Schiff-Alcian Blue (PAS-AB) stains of germ-free and B. dentium mono-associated colon (scale bar = 50 µm). b . Colonic mRNA expression of Muc2 . c . Colonic mRNA expression of IL-10 . d . Serum levels of IL-10 by ELISA. e-g . Colonic mRNA expression of ER-stress related genes (e) GRP-78 , (f) CHOP and (g) sXBP1 . All analyses were performed in germ free (n = 10) and B. dentium mono-associated mice (n = 10). *p

    Article Snippet: IL-10 is commonly produced by dendritic cells and can be produced in response to bacterial stimulation., To determine if B. dentium secreted factors could stimulate IL-10 from immune cells, we generated bone marrow-derived mouse dendritic cells.

    Techniques: Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay