Journal: Frontiers in Immunology
Article Title: Increased Hydrostatic Pressure Promotes Primary M1 Reaction and Secondary M2 Polarization in Macrophages
Figure Lengend Snippet: IL-10 expression in BMDMs under different hydrostatic pressures. (A) Representative immunofluorescence images of IL-10 (red) staining in BMDMs under different hydrostatic pressures. F4/80-positive (green) cells represent macrophages. Scale bar = 50 μm. Graphs displaying the calculated MFI/cell of IL-10 on day 2 (B) and day 7 (C) ( n = 9). Statistical significance between different pressures: * p
Article Snippet: AntibodiesAntibodies for immunofluorescence were purchased from the following sources: F4/80 (0.2 μg/ml, BM8, OriGene, Rockville, MD, USA), TNF-α (2.5 μg/ml, MP6-XT3, eBioscience, San Diego, CA, USA), IL-10 (2.5 μg/ml, JES5-2A5, eBioscience), fibronectin (0.7 μg/ml, Polyclonal, invitrogen, Carlsbad, CA, USA), and collagen IV (2.5 μg/ml, Polyclonal, Invitrogen).
Techniques: Expressing, Immunofluorescence, Staining