Journal: Journal of Veterinary Science

Article Title: Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

doi: 10.4142/jvs.2014.15.1.99

Figure Lengend Snippet: Humoral immune responses in vaccinated piglets from the different groups detected with a commercial ELISA kit.

Article Snippet: To further evaluate cellular immune responses, IFN-γ levels in peripheral blood isolated from the vaccinated piglets at 35 dpi were measured using a commercial ELISA kit (Pig Interferon-γ, IFN-γ ELISA Kit; CUSABIO, USA) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Cell Reports Medicine

Article Title: MYC activation impairs cell-intrinsic IFNγ signaling and confers resistance to anti-PD1/PD-L1 therapy in lung cancer

doi: 10.1016/j.xcrm.2023.101006

Figure Lengend Snippet: Genetic inactivation and expression of IFNγ receptor 1 (IFNGR1) in LC (A) Chromatograms depicting, at the genomic level, the p.Q212X mutation at IFNGR1 in PDC11 cells and the matched normal DNA. The nucleotide change is indicated by an arrow. (B) Western blot of IFNGR1 and PD-L1 in the indicated LC cells. ACTIN and TUBULIN, protein-loading controls. (C) Western blot showing changes in levels of the indicated proteins in the PCD11 cells expressing ectopic wild-type IFNGR1 (IFNGR1-PDC11) on treatment with IFNγ (30 nM), as compared with the empty vector (EV-PDC11) control. Experiments were independently repeated at least twice with similar results. (D) mRNA levels assessed by real-time qPCR of the indicated transcripts (relative to ACTIN ) under basal conditions and after administering IFNγ (30 nM), in H596 cells, in PCD11 parental cells (PDC11), in cells expressing ectopic wild-type IFNGR1 and in EV-PDC11 controls. Results are presented as the median of independent biological triplicates. Asterisks denote statistical significance (∗p < 0.05; ∗∗∗p < 0.005; ∗∗∗∗p < 0.001) from two-tailed Student’s t tests. Data are presented as the mean ± SD. (E) Representative weak and strong immunostaining of IFNGR1 in lung primary tumors. Scale bars, 50 μm. (F) Distribution of the immunostaining of IFNGR1 among lung tumors, by histopathology and levels of HLA-I/B2M and PD-L1. Fisher’s exact test. ns, not significant, LuAD, lung adenocarcinoma; LuSCC, lung squamous cell carcinoma; ns, not significant.

Article Snippet: To determine IFNGR1 levels by immunohistochemistry we used the polyclonal anti-IFNGR1 antibody (10808-1-AP, Proteintech; 1:200 dilution.

Techniques: Expressing, Mutagenesis, Western Blot, Plasmid Preparation, Control, Two Tailed Test, Immunostaining, Histopathology

Journal: Cell Reports Medicine

Article Title: MYC activation impairs cell-intrinsic IFNγ signaling and confers resistance to anti-PD1/PD-L1 therapy in lung cancer

doi: 10.1016/j.xcrm.2023.101006

Figure Lengend Snippet: Oncogenic MYC and low level of constitutive expression of IFNγsign genes (A) Oncoplot of the presence of alterations in the indicated genes. Immunoresponse indicates the presence of mutations of genes involved in immunorecognition (e.g., B2M , TAP1/2 , CALR ) or response to IFNγ ( JAK2 and IFNGR1 ) , and CCLE at cBioPortal. (B) Graphs showing gene expression values in transcripts per million (TPMs) for the transcripts within the IFNγ signature from RNA-seq and at GEO: GSE109720) in (lo)MAX-, (hi)MAX-, and (hi)MYC/MAX-expressing cells. Bars indicate means; differences assessed by two-sided Student’s t test. ns, not significant; S, SCLC. (C) Gene set enrichment analysis (GSEA) showing an inverse correlation in gene expression between the IFNγsign and datasets, including from lungs of mice overexpressing Nmyc (GEO: GSE6077). FDR, false discovery rate; NES, normalized enrichment score.

Article Snippet: To determine IFNGR1 levels by immunohistochemistry we used the polyclonal anti-IFNGR1 antibody (10808-1-AP, Proteintech; 1:200 dilution.

Techniques: Expressing, Gene Expression, RNA Sequencing

Journal: Cell Reports Medicine

Article Title: MYC activation impairs cell-intrinsic IFNγ signaling and confers resistance to anti-PD1/PD-L1 therapy in lung cancer

doi: 10.1016/j.xcrm.2023.101006

Figure Lengend Snippet:

Article Snippet: To determine IFNGR1 levels by immunohistochemistry we used the polyclonal anti-IFNGR1 antibody (10808-1-AP, Proteintech; 1:200 dilution.

Techniques: Virus, Recombinant, Modification, DC Protein Assay, Plasmid Preparation, Sequencing, SYBR Green Assay, Magnetic Beads, Gene Expression, Software