Journal: Pharmaceutical biology

Article Title: Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl 4 /2-AAF.

doi: 10.1080/13880209.2021.1961820

Figure Lengend Snippet: Figure 3. YGJ inhibits the activation of M1 macrophages and non-canonical Wnt signalling pathways and inhibits the differentiation of hepatic progenitor cells into myofibroblasts in vivo. (a) STAT1, IRF3, IRF5, IRF8, and SOCS3 protein bands were depicted in the immunoblot images, and (b) the densitometric quantification of the protein bands presented as a histogram (n ¼ 5 per group). (c) The mRNA expressions of Wnt-4, -5 A, -5B, FZD-2, -3, and -6 in liver were measured by RT-PCR and nor- malized to GAPDH mRNA (n ¼ 5 per group). (d) Wnt5A, Wnt5B, and FZD2 protein bands were depicted in the immunoblot images, and (e) the densitometric quantifi- cation of the protein bands presented as a histogram (n ¼ 5 per group). (f) OV6, SOX9, EpCAM, CK19, and Hep mRNA expressions were measured by RT-PCR and normalized to GAPDH mRNA (n ¼ 5 per group). (g) EpCAM protein bands were depicted in the immunoblot images, and (h) the densitometric quantification of the protein bands presented as a histogram (n ¼ 5 per group). p < 0.05 and p < 0.01. N: untreated group (control); 2-AAF/CCl4: 2-acetylaminofluorene/carbon tetra- chloride-treated group; YGJ: 2-AAF/CCl4 þ Yiguanjian decoction-treated group; SORA: 2-AAF/CCl4 þ sorafenib-treated group.

Article Snippet: Rabbit polyclonal antibodies against Oval Cell Marker (OV6), cytokeratin (CK) 19, signal transducer and activator of transcription (STAT) 5A, STAT5B, STAT6, interferon regulatory factor (IRF) 3, IRF5, IRF8, frizzled (FZD) 2, and LDL receptor-related protein (LRP) 5 were purchased from Proteintech Group Inc. (Chicago, IL, USA).

Techniques: Activation Assay, In Vivo, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Pharmaceutical biology

Article Title: Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl 4 /2-AAF.

doi: 10.1080/13880209.2021.1961820

Figure Lengend Snippet: Figure 4. YGJ inhibits the differentiation of WB-F344 cells into myofibroblasts through inhibiting the activation of M1 macrophages. (a) a-SMA immunofluorescent staining (green) in WB-F344 cells (200) (the presented in vitro experiments were conducted in the same batch, the normal and model groups used the same pictures as published articles. The figures of WB-F344 co-cultured with inactivated macrophages and WB-F344 co-cultured with M1 macrophages were reused form a previous study [Ying Xu et al. 2018] and are reproduced with permission here). (b) a-SMA mRNA expression in WB-F344 cells was measured by RT-PCR and normalized to GAPDH mRNA (n ¼ 3 per group). (c) STAT1, NF-jB, IRF3, IRF5, IRF8, and SOCS3 mRNA expressions in RAW264.7 cells were measured by RT-PCR and normalized to GAPDH mRNA (n ¼ 3 per group); (d) STAT1, NF-jB, IRF3, IRF5, and SOCS3 protein bands were depicted in the immunoblot images, and (e) the densitometric quantification of the protein bands presented as a histogram (n ¼ 3 per group). p < 0.05 and p < 0.01. N: WB-F344 cells co-cultured with inactivated RAW264.7 cells; M: WB-F344 cells co-cultured with LPS (100ng/mL)-activated RAW264.7 cells (referred to as LPS-RAW264.7); YGJ: WB-F344 cells co-cultured with LPS-RAW264.7 treated with Yiguanjian decoction; WIF-1: WB-F344 cells co-cultured with LPS-RAW264.7 treated with Wnt inhibitory factor-1.

Article Snippet: Rabbit polyclonal antibodies against Oval Cell Marker (OV6), cytokeratin (CK) 19, signal transducer and activator of transcription (STAT) 5A, STAT5B, STAT6, interferon regulatory factor (IRF) 3, IRF5, IRF8, frizzled (FZD) 2, and LDL receptor-related protein (LRP) 5 were purchased from Proteintech Group Inc. (Chicago, IL, USA).

Techniques: Activation Assay, Staining, In Vitro, Cell Culture, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: The veterinary quarterly

Article Title: Holstein × Montbéliarde-sired F1 generation crossbred female calves have an increased cellular immune response potential compared with purebred Holsteins.

doi: 10.1080/01652176.2024.2435982

Figure Lengend Snippet: Figure 2. Gating strategies to define bovine T cell subsets and their IFN-γ expression. Mononuclear cells were isolated from blood of 3 months-old female calf and surface stained with antibody cocktails (CD4, CD8, CD44, IFN-γ) after PMA/ionomycin mixture stimulation. The lymphocytes are chosen with FSC versus SSC (A) and then singlets are gated using FSC-a and FSC-H (B). By outputting CD4 versus CD8, CD4+ CD8-, CD8+ CD4- are identified, respectively (C). The CD4+ CD8- T cells expressing IFN-γ (D). The CD8+ CD4- T cells expressing IFN-γ (G). CD4+ CD8- T cells and CD4+ CD8- T cells can be further subdivided into CD44+ and CD44- subsets (E and F), respectively. The lymphocytes expressing IFN-γ (H).

Article Snippet: Monoclonal antibodies (mAb) specific for bovine TCRγδ (WC1), CD8, CD4 and IFN-γ with different fluorochrome conjugates were purchased from BIO-RAD (BIO-RAD, Shanghai, China) and mAb to human CD27, mouse CD44 were purchased from Biolegend (Biolegend, San Diego, USA) (Table 1).

Techniques: Expressing, Isolation, Staining

Journal: The veterinary quarterly

Article Title: Holstein × Montbéliarde-sired F1 generation crossbred female calves have an increased cellular immune response potential compared with purebred Holsteins.

doi: 10.1080/01652176.2024.2435982

Figure Lengend Snippet: Figure 5. Differences of IFN-γ expression in lymphocytes and their T cells of MH and HO calves. Representative dot-plots depict IFN-γ+ T cell subsets in CD4+ (a), CD8+ (B) T cells and total lymphocytes (C). Representative histograms showed the expressions of IFN-γ in lymphocytes (D), CD4+ (E) and CD8+ (F) T cells in the whole blood of MH and HO calves. Data were shown in LSM ± SEM (n = 25). *p < 0.05, **p < 0.01.

Article Snippet: Monoclonal antibodies (mAb) specific for bovine TCRγδ (WC1), CD8, CD4 and IFN-γ with different fluorochrome conjugates were purchased from BIO-RAD (BIO-RAD, Shanghai, China) and mAb to human CD27, mouse CD44 were purchased from Biolegend (Biolegend, San Diego, USA) (Table 1).

Techniques: Expressing

Journal: The veterinary quarterly

Article Title: Holstein × Montbéliarde-sired F1 generation crossbred female calves have an increased cellular immune response potential compared with purebred Holsteins.

doi: 10.1080/01652176.2024.2435982

Figure Lengend Snippet: Figure 6. The expression of IFN-γ was derived from subsets of CD4+ and CD8− T cells, characterized by CD44+ cells rather than CD44− cells. Representative dot-plots depict CD4+ (A) and CD8- (B) T cells subsets of IFN-γ and CD44 from MH (left panel) and HO (right panel) calves. Numbers represent the percentages of cells in each quadrant. Bar graphs showed the expressions of IFN-γ by CD44+ or CD44− subsets in CD4+ (C) and CD8+ (D) T cells of MH and HO calves. Data were shown in LSM ± SEM (n = 25). Labels (a, b, c and d) of different letters indicated a significant difference between any two different sets of data (p < 0.01).

Article Snippet: Monoclonal antibodies (mAb) specific for bovine TCRγδ (WC1), CD8, CD4 and IFN-γ with different fluorochrome conjugates were purchased from BIO-RAD (BIO-RAD, Shanghai, China) and mAb to human CD27, mouse CD44 were purchased from Biolegend (Biolegend, San Diego, USA) (Table 1).

Techniques: Expressing, Derivative Assay