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Image Search Results
Journal: Physiological Reports
Article Title: Erythropoietin and a hypoxia‐inducible factor prolyl hydroxylase inhibitor (HIF‐PHDi) lowers FGF23 in a model of chronic kidney disease (CKD)
doi: 10.14814/phy2.14434
Figure Lengend Snippet: Effect of treatments on iFGF23 and 1,25D‐regulating enzyme expression. (a) Intact FGF23 (iFGF23) in the EPO cohort was markedly elevated in CKD‐saline mice compared to the casein control diet mice. EPO administration in the casein diet group had no effect on FGF23 compared to saline‐injected mice. Importantly, iFGF23 was lowered in CKD mice treated with EPO by over 70%. (b) In the FG cohort, the adenine diet induced iFGF23, which remained elevated in saline‐treated mice. FG‐4592 (“FG”) treatment in CKD mice significantly reduced iFGF23 levels by over 70% compared to saline controls. The dashed line in each graph indicates where diet was switched from 0.2% adenine to 0.15% adenine for the remainder of the study. The shaded area indicates the treatment window with either EPO or FG‐4592. (c) In the EPO‐treated cohort, renal Cyp27b1 mRNA remained unchanged in mice with CKD compared to casein controls, and EPO treatment significantly induced its expression. (d) Cyp24a1 mRNA was increased in mice with CKD compared with controls, and there was no significant difference in expression in the EPO‐CKD mice. (e) In the FG‐4592 treated cohort, Cyp27b1 mRNA was significantly elevated with FG‐4592 treatment in both diets with no difference between the diets. (f) Cyp24a1 expression was elevated in the mice with CKD, and this was reduced to control levels with FG‐4592 treatment ( n = 3–8 mice per group; * p < .05;** p < .01 CKD Saline versus Casein‐Saline, # p < .05; ## p < .01 CKD‐EPO/FG versus CKD Saline via two‐way ANOVA with a Tukey post hoc test or Student's t test where appropriate)
Article Snippet: Serum iFGF23 was assessed using a rodent‐specific commercial ELISA for bioactive,
Techniques: Expressing, Saline, Control, Injection
Journal: Journal of Oral Biology and Craniofacial Research
Article Title: The effect of vitamin D deficiency on the RANKL/OPG ratio in rats
doi: 10.1016/j.jobcr.2022.02.004
Figure Lengend Snippet: Assay Kits used in the study.
Article Snippet:
Techniques: Colorimetric Assay, Calcium Assay, Enzyme-linked Immunosorbent Assay