Journal: Advanced Healthcare Materials

Article Title: Generation of an Induced Pluripotent Stem Cell‐Derived Alveolar Type II In Vitro Model to Study Influenza A Virus Infection and Drug Treatments

doi: 10.1002/adhm.202405141

Figure Lengend Snippet: Differentiation, cultivation, and characterization of iAT2 cells. (a) Overview of the differentiation, cultivation, and plating procedure of iAT2s as described in the methods. iPSCs: induced pluripotent stem cells; iAT2s: iPSC‐derived alveolar type II cells; ALI: air–liquid interface; NKX2.1: NK2 homeobox 1; SP‐C: surface active agent protein C; for StemDiff, DS/SB, CBRa, CK/DCI, CK/DCI+Y: see methods. (b) Representative flow cytometry plots of the lung progenitor markers NKX2.1 coupled to GFP on day 14 (left) and SP‐C coupled to tdTomato on day 30 (right). (c) Representative transmission light image of alveolospheres within matrigel before passaging on day 35. Scale bar = 30 µm. (d) Representative transmission light images of iAT2s seeded on transwell inserts directly after seeding (day X) and 4 or 6 days after seeding (day X + 4 and X + 6). Scale bars = 50 µm. (e) Immunofluorescence images of iAT2s showing expression of antigen Kiel 67 (Ki67; green, left), SP‐C (magenta, left), lysophosphatidylcholine acyltransferase 1 (LPCAT1; yellow, middle), and NKX2.1 (green, right). Nuclei are displayed in blue. Single z‐planes are shown. Scale bars = 20 µm (left, middle) or 50 µm (right).

Article Snippet: Transwell inserts (Greiner Bio‐one, 662 641) were placed in 24‐well plates and coated with 100 μL of Corning Matrigel Human Embryonic Stem Cell‐qualified matrix (Corning, 354 277) according to the manufacturer's instructions.

Techniques: Derivative Assay, Flow Cytometry, Transmission Assay, Passaging, Immunofluorescence, Expressing

Journal: STAR Protocols

Article Title: Integrative protocol for quantifying cholesterol-related sterols in human serum samples and building decision support systems

doi: 10.1016/j.xpro.2024.103213

Figure Lengend Snippet:

Article Snippet: 0.2 mL integ. insert HPLC vials , Various manufacturers , For example: Macherey-Nagel, Cat# 702007.

Techniques: Recombinant, Software, Biomarker Discovery, Mass Spectrometry, Ubiquitin Proteomics

Journal: Molecular and cellular biochemistry

Article Title: Sex-Dimorphic Moderate Hypoglycemia Preconditioning Effects on Hippocampal CA1 Neuron Bio-Energetic and Anti-Oxidant Function

doi: 10.1007/s11010-020-03806-7

Figure Lengend Snippet: Summary of Moderate Hypoglycemia Preconditioning (MH) Effects on Hippocampal CA1 Neuron Glycolytic, Tricarboxylic Acid, Oxidative Respiratory, and Anti-Oxidative Stress Enzyme Responses to Severe Hypoglycemia (SH) in Male versus Female Rats

Article Snippet: Technol.), goat [pyruvate dehydrogenase kinase component E1 (PDK1; CA-46155, 1:1250; ProSci.), IDH2 (CA-42704, 1:1500; ProSci.), ATPs (CA-42704, 1:1500; ProSci.), 4-HNE (NB100–63093, 1:3000, Novus Biol.), or mouse [NT (MAB3248; 1:2000; R&D Research, MN)], Membranes were incubated for 1 hr with peroxidase-conjugated rabbit anti-goat (AP106P, 1:5000; EMD Millipore, Billerica, MA), goat anti-rabbit (NEF812001EA, 1:5000; PerkinElmer, Boston, MA), or goat anti-mouse (NEF822001EA; 1:5000; PerkinElmer) secondary antibodies before exposure to SuperSignal West Femto chemiluminescent substrate (34095; ThermoFisherScientific, Rockford, IL).

Techniques:

Journal: Molecular and cellular biochemistry

Article Title: Sex-Dimorphic Moderate Hypoglycemia Preconditioning Effects on Hippocampal CA1 Neuron Bio-Energetic and Anti-Oxidant Function

doi: 10.1007/s11010-020-03806-7

Figure Lengend Snippet: For each treatment group, triplicate CA1 lysate pools were created for Western blot analysis of AMPK (Figure 5A: male data at left, female data at right), pAMPK (Figure 5B), 4-HNE (Figure 5D), or NT (Figure 5E). Figure 5C presents mean pAMPK/AMPK ratios in male versus female CA1 neurons. Data illustrate mean normalized protein O.D. measures ± S.E.M. for male and female V1−4 (solid white bars), V1−3/SH4 (solid gray bars), and MH1−3/SH4 (cross-hatched gray bars) treatment groups. *p<0.05; **p<0.01; ***p<0.001.

Article Snippet: Technol.), goat [pyruvate dehydrogenase kinase component E1 (PDK1; CA-46155, 1:1250; ProSci.), IDH2 (CA-42704, 1:1500; ProSci.), ATPs (CA-42704, 1:1500; ProSci.), 4-HNE (NB100–63093, 1:3000, Novus Biol.), or mouse [NT (MAB3248; 1:2000; R&D Research, MN)], Membranes were incubated for 1 hr with peroxidase-conjugated rabbit anti-goat (AP106P, 1:5000; EMD Millipore, Billerica, MA), goat anti-rabbit (NEF812001EA, 1:5000; PerkinElmer, Boston, MA), or goat anti-mouse (NEF822001EA; 1:5000; PerkinElmer) secondary antibodies before exposure to SuperSignal West Femto chemiluminescent substrate (34095; ThermoFisherScientific, Rockford, IL).

Techniques: Western Blot