Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: The sequences of primers.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques:

Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: The downregulation of VEGFA expression and suppression of the PI3K/AKT signaling pathway in HemECs by long-pulsed Alexandrite laser (755 nm). (A) Expression analysis of VEGFA, VEGFR2, PI3K, and Akt using quantitative RT-PCR. (B) Western blot analysis of the relative protein expression levels of VEGFA, VEGFR2, p-Akt, and Akt in HemECs. ∗∗ p <0.01, ∗∗∗ p <0.001 vs. control. Abbreviations: HemECs: human hemangioma endothelial cells; VEGF: vascular endothelial growth factor; VEGFR: vascular endothelial growth factor receptor; PI3K: phosphoinositide 3-kinase; Akt: protein kinase B.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: The downregulation of VEGFA expression and suppression of PI3K/AKT signaling pathway activation in xenograft by long-pulsed Alexandrite laser (755 nm). (A) Analysis of the expression levels of VEGFA, VEGFR2, PI3K, and Akt using quantitative RT-PCR. (B) Western blot analysis of the protein expression levels of VEGFA, VEGFR2, p-Akt, and Akt in xenograft. (C) Detection of the relative protein expression levels of VEGFA 165a and VEGFA 165b in xenograft using western blot. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001 vs. control. Abbreviations: VEGF: vascular endothelial growth factor; VEGFR: vascular endothelial growth factor receptor; PI3K: phosphoinositide 3-kinase; Akt: protein kinase B.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques: Expressing, Activation Assay, Quantitative RT-PCR, Western Blot, Control

Journal: Biomedical Journal

Article Title: Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

doi: 10.1016/j.bj.2023.100675

Figure Lengend Snippet: Representative immunohistochemical staining of VEGFA, VEGFR2, PI3K, and Akt in tumor sections from each group of mice (magnification, × 400; scale bar, 25 μm). Abbreviations: VEGF: vascular endothelial growth factor; VEGFR: vascular endothelial growth factor receptor; PI3K: phosphoinositide 3-kinase; Akt: protein kinase B.

Article Snippet: The membranes were gently agitated and incubated with antibodies against the following proteins: VEGFA (BA0407, Boster, Wuhan, China, 1 : 1000), VEGFR2 (A00901-2, Boster, Wuhan, China, 1 : 1000), p-Akt (#4691, Cell Signaling Technology, MA, United States, 1 : 1000), p-Akt (#4060, Cell Signaling Technology, MA, United States, 1 : 2000), VEGF 165a (ab69479, Abcam, Cambridge, MA, United States), VEGF 165b (GTX52502, GeneTex, Irvine, CA, United States), glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (A00227-4, Boster, Wuhan, China, 1 : 1000), at 4 °C overnight.

Techniques: Immunohistochemical staining, Staining