insect cell expression Search Results


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GenScript corporation hpk1 insect cell expression constructs
Hpk1 Insect Cell Expression Constructs, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson human cdna insect cell microsomes expressed cyp isozymes
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
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Corning Life Sciences bd supersomes enzymes baculovirus-insect cell-expressed human uridine diphosphate glucuronosyltransferase (ugt)
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Bd Supersomes Enzymes Baculovirus Insect Cell Expressed Human Uridine Diphosphate Glucuronosyltransferase (Ugt), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Oxford Expression Technologies Ltd baculogrow insect cell medium
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Baculogrow Insect Cell Medium, supplied by Oxford Expression Technologies Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cayman Chemical human 5-lipoxygenase (5-lox) cayman, cat #60402
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Human 5 Lipoxygenase (5 Lox) Cayman, Cat #60402, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences baculovirus-infected insect cell microsomes containing human complementary dna-expressed bcrp
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Baculovirus Infected Insect Cell Microsomes Containing Human Complementary Dna Expressed Bcrp, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Culture Company insect express sf9-s2 medium
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Insect Express Sf9 S2 Medium, supplied by Cell Culture Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation synthesized genes optimized for insect cell expression
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Synthesized Genes Optimized For Insect Cell Expression, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation human ubr5 sequence codon-optimized for insect cell expression with an n-terminal flag tag
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Human Ubr5 Sequence Codon Optimized For Insect Cell Expression With An N Terminal Flag Tag, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Meridian Life Science biotinylated recombinant insect cell-expressed e proteins denv-1 e
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Biotinylated Recombinant Insect Cell Expressed E Proteins Denv 1 E, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotechrabbit spodoptera frugiperda -based cell-free expression system rts insect membrane kit
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Spodoptera Frugiperda Based Cell Free Expression System Rts Insect Membrane Kit, supplied by biotechrabbit, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences baculovirus-infected insect cell microsomes supersomes containing complementary dna-expressed hbcrp (arg482, protein/ml)
Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with <t>cDNA</t> expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 <t>(CYP)</t> 1A1, 1B1, 2J2 and 3A4 isozymes.
Baculovirus Infected Insect Cell Microsomes Supersomes Containing Complementary Dna Expressed Hbcrp (Arg482, Protein/Ml), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with cDNA expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 (CYP) 1A1, 1B1, 2J2 and 3A4 isozymes.

Journal: PLoS ONE

Article Title: Important Role of CYP2J2 in Protein Kinase Inhibitor Degradation: A Possible Role in Intratumor Drug Disposition and Resistance

doi: 10.1371/journal.pone.0095532

Figure Lengend Snippet: Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with cDNA expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 (CYP) 1A1, 1B1, 2J2 and 3A4 isozymes.

Article Snippet: Incubations with a final volume of 100 µL, were performed according to BD Gentest procedure/catalog (BD Bioscience, Belgium) and contained human cDNA insect cell microsomes expressed CYP isozymes and insect cell control microsomes without CYP activities (BD Bioscience, Belgium).

Techniques: In Vitro, Liquid Chromatography with Mass Spectroscopy