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Image Search Results
Journal: PLoS ONE
Article Title: Important Role of CYP2J2 in Protein Kinase Inhibitor Degradation: A Possible Role in Intratumor Drug Disposition and Resistance
doi: 10.1371/journal.pone.0095532
Figure Lengend Snippet: Disappearance of dasatinib, imatinib, nilotinib, sorafenib and Z -sunitinib (each TKI = 2.5 µM at T = 0) from in vitro incubations performed with cDNA expressed CYP1A1, 1B1, 2J2 and 3A4 isozymes. A) Remaining TKI in the microsomal incubations after 30 min., are given in percent of initial TKI levels (T = 0; SD corresponds to the maximum analytical deviation found in the LC-MS assays). Time course of B) dasatinib, C) nilotinib and D) sorafenib degradations in the incubations are depicted for cytochrome P-450 (CYP) 1A1, 1B1, 2J2 and 3A4 isozymes.
Article Snippet: Incubations with a final volume of 100 µL, were performed according to BD Gentest procedure/catalog (BD Bioscience, Belgium) and contained
Techniques: In Vitro, Liquid Chromatography with Mass Spectroscopy