inos Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher inos mm00440502
    Inos Mm00440502, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos mm00440502/product/Thermo Fisher
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    inos mm00440502 - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Taconic Biosciences nos2
    Development of Th1 immunity is compromised in CAT2 −/− mice. A. WT C57BL/6 (open squares), CAT2 −/− (filled circles), <t>NOS2</t> −/− (filled inverted triangles) and IFN-γ −/− (filled triangles) mice were infected i.p. with 20 T. gondii cysts, and survival was assessed up to 50 days post-infection. (N = 5/group). B. Peritoneal exudate cells (PECs) were prepared from WT and CAT2 −/− mice on day 7 post-infection. The percentage of infected cells was determined microscopically by evaluating a minimum of 700 cells per slide (3 mice/group). C. PECs were placed in culture for 24–48 hr and either left untreated or restimulated with STAG. Culture supernatants were collected and IFN-γ levels were determined by ELISA. D. NO activity was evaluated in the same culture supernatants by measuring nitrate production.
    Nos2, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2/product/Taconic Biosciences
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    nos2 - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    76
    AnaSpec inos nos2
    SOD1, SOD2, GPx1, CAT, <t>NOS2</t> and NOS3 mRNA expression in penile rat crura from healthy control, diabetic controls and following Tempol treatment.
    Inos Nos2, supplied by AnaSpec, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos nos2/product/AnaSpec
    Average 76 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    inos nos2 - by Bioz Stars, 2020-03
    76/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology anti nos2 inos
    Immunohistochemical stain of <t>iNOS</t> in the hippocampus and frontal cortex of the four experimental groups. (a, b, c, d) showed the CA3 field in the hippocampus. (e, f, g, h) show the frontal cortex in the same experimental conditions.
    Anti Nos2 Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nos2 inos/product/Santa Cruz Biotechnology
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti nos2 inos - by Bioz Stars, 2020-03
    92/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology nos2 inos
    Immunohistochemical stain of <t>iNOS</t> in the hippocampus and frontal cortex of the four experimental groups. (a, b, c, d) showed the CA3 field in the hippocampus. (e, f, g, h) show the frontal cortex in the same experimental conditions.
    Nos2 Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2 inos/product/Santa Cruz Biotechnology
    Average 86 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    nos2 inos - by Bioz Stars, 2020-03
    86/100 stars
      Buy from Supplier

    77
    Pharmingen nos2 inos
    A/ Growth rate and survival of RAW 264.7 cells cultured in medium, or medium supplemented with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells were cultured (4 × 10 6 cells in 20 ml) in medium (• œ) or medium supplemented with either IFN-γ (50 U/ml -.), or LPS (5 μg/ml -) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml -). The number of live cells recovered at the indicated times was estimated using trypan blue exclusion. Similar results were obtained estimating the cell survival either by 3H-thymidine incorporation, or by the MTT reduction test. B/ <t>NOS2</t> <t>(iNOs)</t> mRNA induction in RAW 264.7 cultured with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells seeded into six-well plates (2.5 × 10 6 cells/well) were treated with the different stimuli as indicated above for the indicated times and analyzed by RT-PCR with specific primers for murine NOS2 (iNOs) (Table 1 ). HPRT was used as internal control for semi-quantitative estimation. C/ NOS2 (iNOs) protein in RAW 264.7 cultured with LPS or IFN-ã or LPS+IFN-γ. RAW 264.7 cells were treated with the different stimuli as indicated above for various times and cell pellets (1 × 10 6 cells) were lysed with lysis buffer. Protein concentrations in samples were adjusted and electrophoresed on 15% SDS-polyacrylamide gel, then transferred to nitro-cellulose membrane and Western bloted using polyclonal rabbit anti-murine NOS2 (iNOs), as described in Materials and Methods.
    Nos2 Inos, supplied by Pharmingen, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2 inos/product/Pharmingen
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nos2 inos - by Bioz Stars, 2020-03
    77/100 stars
      Buy from Supplier

    89
    Millipore inos nos2
    A/ Growth rate and survival of RAW 264.7 cells cultured in medium, or medium supplemented with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells were cultured (4 × 10 6 cells in 20 ml) in medium (• œ) or medium supplemented with either IFN-γ (50 U/ml -.), or LPS (5 μg/ml -) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml -). The number of live cells recovered at the indicated times was estimated using trypan blue exclusion. Similar results were obtained estimating the cell survival either by 3H-thymidine incorporation, or by the MTT reduction test. B/ <t>NOS2</t> <t>(iNOs)</t> mRNA induction in RAW 264.7 cultured with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells seeded into six-well plates (2.5 × 10 6 cells/well) were treated with the different stimuli as indicated above for the indicated times and analyzed by RT-PCR with specific primers for murine NOS2 (iNOs) (Table 1 ). HPRT was used as internal control for semi-quantitative estimation. C/ NOS2 (iNOs) protein in RAW 264.7 cultured with LPS or IFN-ã or LPS+IFN-γ. RAW 264.7 cells were treated with the different stimuli as indicated above for various times and cell pellets (1 × 10 6 cells) were lysed with lysis buffer. Protein concentrations in samples were adjusted and electrophoresed on 15% SDS-polyacrylamide gel, then transferred to nitro-cellulose membrane and Western bloted using polyclonal rabbit anti-murine NOS2 (iNOs), as described in Materials and Methods.
    Inos Nos2, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos nos2/product/Millipore
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    inos nos2 - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc inos antibodies
    PDE5 and <t>iNOS</t> expression in human endothelial cells. A : PDE5 mRNA expression in HUVEC and hfCC cells: RT-PCR shows that HUVEC express detectable levels of PDE-5 mRNA. Actin was used as control. B : Western Blot analysis for PDE5 protein expression. C : Real time PCR shows that insulin (30 min) and/or sildenafil (5 h) treatments induce PDE5 and <t>eNOS</t> but not iNOS expression * p
    Inos Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos antibodies/product/Cell Signaling Technology Inc
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    inos antibodies - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    82
    Enzo Biochem inos nos2 inhibitor l nil
    M-MDSCs derived earlier in the course of LP-BM5 infection exhibit decreased suppression of T-cell responses ). Histograms (A-E) represent the means of triplicate samples with standard deviations of a representative experiment, of a total of at least three independent experiments. The percent suppression of B-cell (A) or T-cell (C) responsiveness by M-MDSCs is depicted for both standard co-cultures and co-cultures with the addition of the <t>iNOS</t> inhibitor, <t>L-NIL.</t> The effect on suppression (blockade) by L-NIL addition on B-cell (B) versus T-cell (D) responsiveness is compared. (E) Supernates derived from parallel suppression assays in which responder cells were stimulated with anti-CD3/CD28, in the absence or presence of M-MDSCs, were assessed for nitric oxide (NO) using the Griess reagent for nitrite. *p
    Inos Nos2 Inhibitor L Nil, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos nos2 inhibitor l nil/product/Enzo Biochem
    Average 82 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    inos nos2 inhibitor l nil - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    99
    ABclonal antibodies against inos
    M-MDSCs derived earlier in the course of LP-BM5 infection exhibit decreased suppression of T-cell responses ). Histograms (A-E) represent the means of triplicate samples with standard deviations of a representative experiment, of a total of at least three independent experiments. The percent suppression of B-cell (A) or T-cell (C) responsiveness by M-MDSCs is depicted for both standard co-cultures and co-cultures with the addition of the <t>iNOS</t> inhibitor, <t>L-NIL.</t> The effect on suppression (blockade) by L-NIL addition on B-cell (B) versus T-cell (D) responsiveness is compared. (E) Supernates derived from parallel suppression assays in which responder cells were stimulated with anti-CD3/CD28, in the absence or presence of M-MDSCs, were assessed for nitric oxide (NO) using the Griess reagent for nitrite. *p
    Antibodies Against Inos, supplied by ABclonal, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against inos/product/ABclonal
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against inos - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher nos2
    AAV delivered <t>iNOS</t> results in NO production in a dose and time-dependent manner
    Nos2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2/product/Thermo Fisher
    Average 99 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    nos2 - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    78
    The Jackson Laboratory inos nos2 tm1lau mice
    AAV delivered <t>iNOS</t> results in NO production in a dose and time-dependent manner
    Inos Nos2 Tm1lau Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos nos2 tm1lau mice/product/The Jackson Laboratory
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    inos nos2 tm1lau mice - by Bioz Stars, 2020-03
    78/100 stars
      Buy from Supplier

    98
    Abbexa anti inos
    AAV delivered <t>iNOS</t> results in NO production in a dose and time-dependent manner
    Anti Inos, supplied by Abbexa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti inos/product/Abbexa
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti inos - by Bioz Stars, 2020-03
    98/100 stars
      Buy from Supplier

    inos  (Abcam)
    99
    Abcam inos
    Effects of myeloid cell mPGES-1 deletion on macrophage abundance. ( A ) Lesion morphology analysis in aortic roots from mice on a HFD for 3 mo. Shown are representative images for <t>CD11b</t> ( a ), <t>iNOS</t> ( c ), and CD206 ( e ) staining in Mac-mPGES-1-WT aortic root
    Inos, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1938 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos/product/Abcam
    Average 99 stars, based on 1938 article reviews
    Price from $9.99 to $1999.99
    inos - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    91
    OriGene inos
    Nitrotyrosine formation in (a) RAW 264.7 cells, (b) RAW 264.7 cells stimulated with LPS, (c) LPS-stimulated RAW 264.7 cells treated with L-NAME (25 μ M), (d) <t>iNOS</t> −/− RAW 264.7 cells, (e) iNOS −/− RAW 264.7 cells stimulated with LPS, and (f) RAW 264.7 cells <t>transfected</t> with negative control plasmid stimulated with LPS. Cells were incubated in the presence of DMEM media supplemented with 400 μ M of L-arginine.
    Inos, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos/product/OriGene
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    inos - by Bioz Stars, 2020-03
    91/100 stars
      Buy from Supplier

    99
    Abcam nos2 inos
    Genetic depletion of HIF-1 α in astrocytes decreases expression of HIF-1 α and <t>iNOS</t> in astrocytes. A , Staining for HIF-1 α in <t>GFAP-Cre:HIF-1α</t> fl/fl and HIF-1α fl/fl mice after EAE. B , Quantification of iNOS expression in Mac-2+ macrophages and GFAP-expressing astrocytes at peak EAE in HIF-1α fl/fl mice ( n = 5). Area = 0.056 mm 2 . Data are presented as mean ± SEM from n = 5 mice. Statistical analysis was performed using an unpaired t -test with Welch’s correction ( p = 0.003 ° ). C , Quantification iNOS+ cells at peak EAE in HIF-1α fl/fl ( n =5) and GFAP-Cre:HIF-1α fl/fl ( n =4) mice. Area =0.056 mm 2 . Data are presented as mean ±SEM. Statistical analysis was performed an unpaired t test ( p = 0.034 p ). D , Double-immunofluorescence of GFAP (green) and iNOS (red) in HIF-1α fl/fl and GFAP-Cre:HIF-1α fl/fl mice at peak EAE. Statistical analysis was performed using an unpaired t test ( p = 0.01 q ). E , Quantification of iNOS+ GFAP+ cells reveals reduced iNOS-expressing astrocytes in GFAP-Cre:HIF-1α fl/fl ( n = 4) compared with HIF-1α fl/fl ( n = 5) mice. Area = 0.056 mm 2 . Statistical analysis was performed using an unpaired t test ( p = 0.0159 q ). F , Quantification of GFAP+ astrocytes shows similar numbers of astrocytes in GFAP-Cre:HIF-1α fl/fl ( n = 4) and HIF-1α fl/fl ( n = 5) mice at peak EAE. Area = 0.056 mm 2 . Data are presented as mean ± SEM. Statistical analysis was performed using an unpaired t test ( p = 0.06 r ). Superscript letters refer to the statistical results in Tables 1 , 2 , and Results.
    Nos2 Inos, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2 inos/product/Abcam
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    nos2 inos - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc nos2
    Effects of aerobic exercise on cardiac <t>NOS2</t> and NOS1 expression after MI. A. Western blot analysis of cardiac NOS2 and p-NOS2 in the SC, MI, ME groups. The expression of total NOS2 and p-NOS2 remained constant in the SC and MI groups. Compared with the MI group, NOS2 was significantly activated in the ME group. B. Western blot analysis of cardiac NOS1 in the SC, MI, ME groups. The protein expression of NOS1 was significantly higher in the ME group compared with the SC and MI groups.
    Nos2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2/product/Cell Signaling Technology Inc
    Average 95 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    nos2 - by Bioz Stars, 2020-03
    95/100 stars
      Buy from Supplier

    78
    The Jackson Laboratory inos deficient nos2
    Representative histochemical ( a–d ) and immunohistochemical ( e,f ) staining in control ( a,c ) and injured ( b,d,e,f ) carotid arteries from C57BL/6 <t>iNOS-null</t> mice. Hematoxylin and eosin staining ( a,b ) indicates a cellular thickening in the injured
    Inos Deficient Nos2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos deficient nos2/product/The Jackson Laboratory
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    inos deficient nos2 - by Bioz Stars, 2020-03
    78/100 stars
      Buy from Supplier

    82
    Santa Cruz Biotechnology m1 macrophage markers nos2a inos
    Cancer-adjacent normal tissues express a similar in vivo pattern of macrophage polarization distinguishing BBC from luminal breast cancer (A) H E stained sections for tissues adjacent to BBC or luminal breast cancer and dual stained immunofluorescent images with staining for M1/IL-7R (red) and M2/CD36 (green). (B) H E sections for the same specimens, but with dual staining for <t>M1/iNOS</t> (blue) and M2/CD163 (red). More dual stained M1/M2 macrophages and higher numbers of M2 positive macrophages are observed in the BBC-adjacent tissues in this set of samples.
    M1 Macrophage Markers Nos2a Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m1 macrophage markers nos2a inos/product/Santa Cruz Biotechnology
    Average 82 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m1 macrophage markers nos2a inos - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    86
    Elabscience rat nos2 inos
    Cancer-adjacent normal tissues express a similar in vivo pattern of macrophage polarization distinguishing BBC from luminal breast cancer (A) H E stained sections for tissues adjacent to BBC or luminal breast cancer and dual stained immunofluorescent images with staining for M1/IL-7R (red) and M2/CD36 (green). (B) H E sections for the same specimens, but with dual staining for <t>M1/iNOS</t> (blue) and M2/CD163 (red). More dual stained M1/M2 macrophages and higher numbers of M2 positive macrophages are observed in the BBC-adjacent tissues in this set of samples.
    Rat Nos2 Inos, supplied by Elabscience, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat nos2 inos/product/Elabscience
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rat nos2 inos - by Bioz Stars, 2020-03
    86/100 stars
      Buy from Supplier

    90
    Becton Dickinson inos no
    Cancer-adjacent normal tissues express a similar in vivo pattern of macrophage polarization distinguishing BBC from luminal breast cancer (A) H E stained sections for tissues adjacent to BBC or luminal breast cancer and dual stained immunofluorescent images with staining for M1/IL-7R (red) and M2/CD36 (green). (B) H E sections for the same specimens, but with dual staining for <t>M1/iNOS</t> (blue) and M2/CD163 (red). More dual stained M1/M2 macrophages and higher numbers of M2 positive macrophages are observed in the BBC-adjacent tissues in this set of samples.
    Inos No, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos no/product/Becton Dickinson
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    inos no - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

    99
    Biorbyt inos antibody
    Effects of IVIg treatment in inflammatory infiltration in peripheral nerves. a Immunohistochemistry for <t>CD68:</t> After 3 and 8 weeks of BTZ treatment, both sciatic and caudal nerves show a massive macrophage infiltration which is almost completely abrogated by the preventive co-treatment with IVIg (BTZ + IVIg 3; BTZ + IVIg 8). In the therapeutic setting (BTZ + IVIg 4), a milder reduction of CD68 + infiltrating cells was achieved in both sciatic and caudal nerves. Scale bar 50 μm. b Representative immunohistochemistry for <t>iNOS</t> and ARG1 after 3 weeks of BTZ treatment. Most infiltrating macrophages (CD68+) are iNOS + pro-inflammatory M1 type while a very limited amount of them are ARG1+ anti-inflammatory M2 type. Scale bar 50 μm
    Inos Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos antibody/product/Biorbyt
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    inos antibody - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology nos2 pe
    IFNγ and TNFα are critical mediators of MDSC hyper-activation induced by type-1 immune effector cells. (A) Expression of IDO1, <t>NOS2,</t> IL10, and COX2 in OvCa ascites-isolated MDSCs cultured for 24 h with or without IL18/IFNα-activated
    Nos2 Pe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2 pe/product/Santa Cruz Biotechnology
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    nos2 pe - by Bioz Stars, 2020-03
    85/100 stars
      Buy from Supplier

    79
    Santa Cruz Biotechnology goat polyclonal anti inos nos2
    IFNγ and TNFα are critical mediators of MDSC hyper-activation induced by type-1 immune effector cells. (A) Expression of IDO1, <t>NOS2,</t> IL10, and COX2 in OvCa ascites-isolated MDSCs cultured for 24 h with or without IL18/IFNα-activated
    Goat Polyclonal Anti Inos Nos2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti inos nos2/product/Santa Cruz Biotechnology
    Average 79 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    goat polyclonal anti inos nos2 - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology nos1 nos2
    Renal nitric oxide synthase (NOS) protein abundance and urinary nitrite/nitrate concentration. 5A . Immunoblots of <t>NOS1,</t> <t>NOS2,</t> and NOS3 in whole kidney homogenates (n=5/group). 5B . Densitometric analysis of the immunoblots. On NS (0.8% NaCl) diet, the renal protein abundances of NOS1 and NOS2 in GRK4γ486V (486V) transgenic mice and Non-transgenic (Non-T) littermates are similar and not altered by HS (6% NaCl) diet. NOS3 protein abundance is similar in 486V and Non-T mice on NS diet but is decreased by HS diet in 486V (49±2%) but not Non-T mice. *P
    Nos1 Nos2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos1 nos2/product/Santa Cruz Biotechnology
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    nos1 nos2 - by Bioz Stars, 2020-03
    85/100 stars
      Buy from Supplier

    99
    Millipore nos2
    Differences in localization patterns of Actin, Tubulin, CD11b and E-Selectin in APECs from C57BL/6 and <t>Nos2</t> -/- APECs upon infection. Fluorescence microscopic images of F-Actin (green) and α-Tubulin (red) in APECs from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (A). Yellow and white arrows represent Actin and Tubulin localization to the cortex, respectively. Confocal microscopic images of E-Selectin (green) and CD11b (red) in APECs taken at 100X magnification from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (B). The white arrows depict CD11b localization at the cell-cell interface in C57BL/6 APECs from infected mice. On the other hand, APECs from infected Nos2 -/- mice display diffused CD11b pattern. Scale bar for fluorescent microscopic images is 10 μm. The data is presented as mean ± S.E from two independent representative experiments with three mice per group.
    Nos2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2/product/Millipore
    Average 99 stars, based on 436 article reviews
    Price from $9.99 to $1999.99
    nos2 - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher nos2 cxnft antibodies
    Differences in localization patterns of Actin, Tubulin, CD11b and E-Selectin in APECs from C57BL/6 and <t>Nos2</t> -/- APECs upon infection. Fluorescence microscopic images of F-Actin (green) and α-Tubulin (red) in APECs from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (A). Yellow and white arrows represent Actin and Tubulin localization to the cortex, respectively. Confocal microscopic images of E-Selectin (green) and CD11b (red) in APECs taken at 100X magnification from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (B). The white arrows depict CD11b localization at the cell-cell interface in C57BL/6 APECs from infected mice. On the other hand, APECs from infected Nos2 -/- mice display diffused CD11b pattern. Scale bar for fluorescent microscopic images is 10 μm. The data is presented as mean ± S.E from two independent representative experiments with three mice per group.
    Nos2 Cxnft Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2 cxnft antibodies/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nos2 cxnft antibodies - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    80
    Santa Cruz Biotechnology inos antiserum
    A schematic representation of the relaxin pathway when disturbed by simazine. Simazine inhibits testicular Rxfp1 expression, which consequently may limit the intracellular signaling of relaxin in the testis. The compromised relaxin-Rxfp1 signaling is further expected to downregulate the expression of relaxin target genes such as <t>Nos2,</t> Nos3, Vegf, and Mmp9, leading to the inhibition of NO release.
    Inos Antiserum, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos antiserum/product/Santa Cruz Biotechnology
    Average 80 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    inos antiserum - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    89
    BioLegend inos apc
    A schematic representation of the relaxin pathway when disturbed by simazine. Simazine inhibits testicular Rxfp1 expression, which consequently may limit the intracellular signaling of relaxin in the testis. The compromised relaxin-Rxfp1 signaling is further expected to downregulate the expression of relaxin target genes such as <t>Nos2,</t> Nos3, Vegf, and Mmp9, leading to the inhibition of NO release.
    Inos Apc, supplied by BioLegend, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/inos apc/product/BioLegend
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    inos apc - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    80
    The Jackson Laboratory nos2 inos knockout mice
    Model for the potentiation of TNF α -induced hepatotoxicity, oxidative stress mitochondrial dysfunction, and activation of MAPK by pyrazole induction of CYP2E1. Pyrazole induction of CYP2E1 coupled to TNF α induction of <t>iNOS</t> results in elevated oxidative/nitrosative stress in hepatocytes. This results in activation of JNK and p38 MAPK which, along with the elevated ROS/RNS, damage mitochondrial function ultimately leading to liver injury.
    Nos2 Inos Knockout Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nos2 inos knockout mice/product/The Jackson Laboratory
    Average 80 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    nos2 inos knockout mice - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

    82
    Santa Cruz Biotechnology antibodies against rabbit polyclonal nos2 inos
    May Grünwald-Giemsa-stained lymphocyte of a control subject ( a ); and a confocal image showing inducible Nitric Oxide Synthase <t>(iNOS)</t> positivity ( b ); and cytochrome C positivity ( c ) lymphocytes of a placebo treated critically-ill patient. Scale bar = 20μm.
    Antibodies Against Rabbit Polyclonal Nos2 Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 82/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against rabbit polyclonal nos2 inos/product/Santa Cruz Biotechnology
    Average 82 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    antibodies against rabbit polyclonal nos2 inos - by Bioz Stars, 2020-03
    82/100 stars
      Buy from Supplier

    Image Search Results


    Development of Th1 immunity is compromised in CAT2 −/− mice. A. WT C57BL/6 (open squares), CAT2 −/− (filled circles), NOS2 −/− (filled inverted triangles) and IFN-γ −/− (filled triangles) mice were infected i.p. with 20 T. gondii cysts, and survival was assessed up to 50 days post-infection. (N = 5/group). B. Peritoneal exudate cells (PECs) were prepared from WT and CAT2 −/− mice on day 7 post-infection. The percentage of infected cells was determined microscopically by evaluating a minimum of 700 cells per slide (3 mice/group). C. PECs were placed in culture for 24–48 hr and either left untreated or restimulated with STAG. Culture supernatants were collected and IFN-γ levels were determined by ELISA. D. NO activity was evaluated in the same culture supernatants by measuring nitrate production.

    Journal: PLoS Pathogens

    Article Title: Cationic Amino Acid Transporter-2 Regulates Immunity by Modulating Arginase Activity

    doi: 10.1371/journal.ppat.1000023

    Figure Lengend Snippet: Development of Th1 immunity is compromised in CAT2 −/− mice. A. WT C57BL/6 (open squares), CAT2 −/− (filled circles), NOS2 −/− (filled inverted triangles) and IFN-γ −/− (filled triangles) mice were infected i.p. with 20 T. gondii cysts, and survival was assessed up to 50 days post-infection. (N = 5/group). B. Peritoneal exudate cells (PECs) were prepared from WT and CAT2 −/− mice on day 7 post-infection. The percentage of infected cells was determined microscopically by evaluating a minimum of 700 cells per slide (3 mice/group). C. PECs were placed in culture for 24–48 hr and either left untreated or restimulated with STAG. Culture supernatants were collected and IFN-γ levels were determined by ELISA. D. NO activity was evaluated in the same culture supernatants by measuring nitrate production.

    Article Snippet: 20 cysts of the avirulent ME49 strain were inoculated i.p into C57BL/6, CAT2−/− , NOS2−/− (Taconic), and IFN-γ−/− (Taconic) mice for morbundity studies.

    Techniques: Mouse Assay, Infection, Enzyme-linked Immunosorbent Assay, Activity Assay

    Effect of iNOS on growth of B. abortus 2308 and the virB mutant in mouse spleens. Groups of 10 C57BL/6 mice and 10 Nos2 knockout mice were inoculated i.p. with a mixture containing equal amounts of B. abortus 2308 and the virB mutant. The CFU of each strain were enumerated in the spleens of five mice from each group at 14 days and 60 days postinoculation. For each mouse, the results are indicated by one circle (log CFU of the wild type) and one square (log CFU of the virB mutant). The open circles indicate log CFU of B. abortus 2308 recovered from the spleens of C57BL/6 mice, and the open squares indicate log CFU of the virB mutant recovered from the spleens of C57BL/6 mice. The solid circles indicate log CFU of B. abortus 2308 recovered from Nos2 knockout mice, and the solid squares indicate log CFU of the virB mutant recovered from the spleens of Nos2 knockout mice. The means of the data are indicated by horizontal lines. The competitive index was calculated by dividing the mean of mutant CFU recovered from spleens/wild-type CFU recovered from spleens by the ratio of the mutant to the wild type in the inoculum. k/o, knockout.

    Journal: Infection and Immunity

    Article Title: virB-Mediated Survival of Brucella abortus in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

    doi: 10.1128/IAI.70.9.4826-4832.2002

    Figure Lengend Snippet: Effect of iNOS on growth of B. abortus 2308 and the virB mutant in mouse spleens. Groups of 10 C57BL/6 mice and 10 Nos2 knockout mice were inoculated i.p. with a mixture containing equal amounts of B. abortus 2308 and the virB mutant. The CFU of each strain were enumerated in the spleens of five mice from each group at 14 days and 60 days postinoculation. For each mouse, the results are indicated by one circle (log CFU of the wild type) and one square (log CFU of the virB mutant). The open circles indicate log CFU of B. abortus 2308 recovered from the spleens of C57BL/6 mice, and the open squares indicate log CFU of the virB mutant recovered from the spleens of C57BL/6 mice. The solid circles indicate log CFU of B. abortus 2308 recovered from Nos2 knockout mice, and the solid squares indicate log CFU of the virB mutant recovered from the spleens of Nos2 knockout mice. The means of the data are indicated by horizontal lines. The competitive index was calculated by dividing the mean of mutant CFU recovered from spleens/wild-type CFU recovered from spleens by the ratio of the mutant to the wild type in the inoculum. k/o, knockout.

    Article Snippet: C57BL/6 Ai-[KO]iNOS N5 mice carrying a targeted knockout of the Nos2 gene encoding iNOS were obtained from Taconic (Germantown, N.Y.) ( ).

    Techniques: Mutagenesis, Mouse Assay, Knock-Out

    Survival of B. abortus 2308 and the isogenic virB mutant BA41 in elicited murine peritoneal macrophages. Macrophages were either not treated (-), treated with the iNOS inhibitor MMLA, activated with 20 U of murine IFN-γ per ml and 1 pg of LPS per ml, or activated and treated with MMLA. The solid bars indicate the number of intracellular CFU of B. abortus 2308 per well, and the open bars indicate the number of CFU of BA41 ( virB ) at 24 h postinoculation. Each bar indicates the mean ± standard deviation for triplicate samples from a single experiment. Statistical analysis was performed by using a Student's t test. The same letter above bars indicates that differences were not statistically significant ( P > 0.05). Different letters above bars indicate that differences were statistically significant at P

    Journal: Infection and Immunity

    Article Title: virB-Mediated Survival of Brucella abortus in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

    doi: 10.1128/IAI.70.9.4826-4832.2002

    Figure Lengend Snippet: Survival of B. abortus 2308 and the isogenic virB mutant BA41 in elicited murine peritoneal macrophages. Macrophages were either not treated (-), treated with the iNOS inhibitor MMLA, activated with 20 U of murine IFN-γ per ml and 1 pg of LPS per ml, or activated and treated with MMLA. The solid bars indicate the number of intracellular CFU of B. abortus 2308 per well, and the open bars indicate the number of CFU of BA41 ( virB ) at 24 h postinoculation. Each bar indicates the mean ± standard deviation for triplicate samples from a single experiment. Statistical analysis was performed by using a Student's t test. The same letter above bars indicates that differences were not statistically significant ( P > 0.05). Different letters above bars indicate that differences were statistically significant at P

    Article Snippet: C57BL/6 Ai-[KO]iNOS N5 mice carrying a targeted knockout of the Nos2 gene encoding iNOS were obtained from Taconic (Germantown, N.Y.) ( ).

    Techniques: Mutagenesis, Standard Deviation

    TNF and cell contact trigger NOS2 expression in FRCs. (a) Nitric oxide in supernatants of wild-type FRCs (5 × 10 4 ) cultured for 48 h with or without (No stimuli) recombinant IFN-γ (50 ng/ml) or recombinant TNF (50 ng/ml). (b) Quantitative

    Journal: Nature immunology

    Article Title: Regulated release of nitric oxide by nonhematopoietic stroma controls expansion of the activated T cell pool in lymph nodes

    doi: 10.1038/ni.2112

    Figure Lengend Snippet: TNF and cell contact trigger NOS2 expression in FRCs. (a) Nitric oxide in supernatants of wild-type FRCs (5 × 10 4 ) cultured for 48 h with or without (No stimuli) recombinant IFN-γ (50 ng/ml) or recombinant TNF (50 ng/ml). (b) Quantitative

    Article Snippet: Nos2 −/− mice were from Taconic; Ifngr1 −/− , Ifng −/− and Tnfrsf1a −/− Tnfrsf1b −/− mice were from Jackson Laboratories.

    Techniques: Expressing, Cell Culture, Recombinant

    NOS2 is expressed by FRCs and LECs in vivo . Microscopy of cryosections of SLNs (a) and MLNs (b) from iFABP-tOVA mice given no cells (iFABP-tOVA (no OT-I)) or 1.5 × 10 6 CD45.1 + congenic OT-I T cells (iFABP-tOVA + OT-I), assessed 48 h later by staining

    Journal: Nature immunology

    Article Title: Regulated release of nitric oxide by nonhematopoietic stroma controls expansion of the activated T cell pool in lymph nodes

    doi: 10.1038/ni.2112

    Figure Lengend Snippet: NOS2 is expressed by FRCs and LECs in vivo . Microscopy of cryosections of SLNs (a) and MLNs (b) from iFABP-tOVA mice given no cells (iFABP-tOVA (no OT-I)) or 1.5 × 10 6 CD45.1 + congenic OT-I T cells (iFABP-tOVA + OT-I), assessed 48 h later by staining

    Article Snippet: Nos2 −/− mice were from Taconic; Ifngr1 −/− , Ifng −/− and Tnfrsf1a −/− Tnfrsf1b −/− mice were from Jackson Laboratories.

    Techniques: In Vivo, Microscopy, Mouse Assay, Staining

    NOS2-mediated suppression operates in vivo . (a) Division of CFSE-labeled OT-I T cells (5 × 10 5 ) cultured for 48 h with OVA peptide–loaded DCs or wild-type or Nos2 −/− FRCs (5 × 10 4 ). (b) CSFE dilution by T cells

    Journal: Nature immunology

    Article Title: Regulated release of nitric oxide by nonhematopoietic stroma controls expansion of the activated T cell pool in lymph nodes

    doi: 10.1038/ni.2112

    Figure Lengend Snippet: NOS2-mediated suppression operates in vivo . (a) Division of CFSE-labeled OT-I T cells (5 × 10 5 ) cultured for 48 h with OVA peptide–loaded DCs or wild-type or Nos2 −/− FRCs (5 × 10 4 ). (b) CSFE dilution by T cells

    Article Snippet: Nos2 −/− mice were from Taconic; Ifngr1 −/− , Ifng −/− and Tnfrsf1a −/− Tnfrsf1b −/− mice were from Jackson Laboratories.

    Techniques: In Vivo, Labeling, Cell Culture

    FRCs use NOS2 to regulate T cell proliferation. (a) Quantitative RT-PCR analysis of Arg1 , Ido1 and Nos2 mRNA in FRC populations expanded ex vivo in the presence of recombinant IFN-γ (50 ng/ml); results are presented relative to those of untreated

    Journal: Nature immunology

    Article Title: Regulated release of nitric oxide by nonhematopoietic stroma controls expansion of the activated T cell pool in lymph nodes

    doi: 10.1038/ni.2112

    Figure Lengend Snippet: FRCs use NOS2 to regulate T cell proliferation. (a) Quantitative RT-PCR analysis of Arg1 , Ido1 and Nos2 mRNA in FRC populations expanded ex vivo in the presence of recombinant IFN-γ (50 ng/ml); results are presented relative to those of untreated

    Article Snippet: Nos2 −/− mice were from Taconic; Ifngr1 −/− , Ifng −/− and Tnfrsf1a −/− Tnfrsf1b −/− mice were from Jackson Laboratories.

    Techniques: Quantitative RT-PCR, Ex Vivo, Recombinant

    LECs inhibit T cell proliferation via NOS2. (a) Division of splenocytes (1 × 10 6 ) activated for 48 h in the presence (LEC) or absence (No LEC) of LEC populations expanded ex vivo (4.5 × 10 4 LECs). Each symbol represents an individual well;

    Journal: Nature immunology

    Article Title: Regulated release of nitric oxide by nonhematopoietic stroma controls expansion of the activated T cell pool in lymph nodes

    doi: 10.1038/ni.2112

    Figure Lengend Snippet: LECs inhibit T cell proliferation via NOS2. (a) Division of splenocytes (1 × 10 6 ) activated for 48 h in the presence (LEC) or absence (No LEC) of LEC populations expanded ex vivo (4.5 × 10 4 LECs). Each symbol represents an individual well;

    Article Snippet: Nos2 −/− mice were from Taconic; Ifngr1 −/− , Ifng −/− and Tnfrsf1a −/− Tnfrsf1b −/− mice were from Jackson Laboratories.

    Techniques: Ex Vivo

    SOD1, SOD2, GPx1, CAT, NOS2 and NOS3 mRNA expression in penile rat crura from healthy control, diabetic controls and following Tempol treatment.

    Journal: International journal of impotence research

    Article Title: Superoxide dismutase analog (TEMPOL: 4-hydroxy-2, 2, 6, 6-tetramethylpiperidine 1-oxyl) treatment restores erectile function in diabetes-induced impotence

    doi: 10.1038/ijir.2009.28

    Figure Lengend Snippet: SOD1, SOD2, GPx1, CAT, NOS2 and NOS3 mRNA expression in penile rat crura from healthy control, diabetic controls and following Tempol treatment.

    Article Snippet: To confirm the RT-PCR results, we performed IHC for catalase (CAT) (#ab52477, Abcam, Cambridge, MA) and iNOS (NOS2) (#53584, Anaspec, San Jose, CA).

    Techniques: Expressing

    Mean quantitative gene expression of SOD1 (A), SOD2 (B), GPx1 (C), CAT (D), NOS2 (E) and NOS3 (F) was analyzed using densitometry. In SOD1 mRNA expression there was no significant change among healthy controls, DM controls and the Tempol treatment group. However SOD2 mRNA expression in DM was significantly lower than healthy control’s and Tempol treatment partially restored its expression but did not reach significance. Glutathione peroxidase (GPX1) mRNA expression (C) was similar in all groups. CAT mRNA expression (D) in DM was significantly lower than in healthy controls and Tempol treatment restored CAT to healthy control level. NOS2 mRNA expression (E) in DM was significantly higher than healthy controls and Tempol treatment mRNA was lower than healthy controls. In NOS3 mRNA expression (F) there was no significant change among diabetes, healthy control and Tempol treatment group.

    Journal: International journal of impotence research

    Article Title: Superoxide dismutase analog (TEMPOL: 4-hydroxy-2, 2, 6, 6-tetramethylpiperidine 1-oxyl) treatment restores erectile function in diabetes-induced impotence

    doi: 10.1038/ijir.2009.28

    Figure Lengend Snippet: Mean quantitative gene expression of SOD1 (A), SOD2 (B), GPx1 (C), CAT (D), NOS2 (E) and NOS3 (F) was analyzed using densitometry. In SOD1 mRNA expression there was no significant change among healthy controls, DM controls and the Tempol treatment group. However SOD2 mRNA expression in DM was significantly lower than healthy control’s and Tempol treatment partially restored its expression but did not reach significance. Glutathione peroxidase (GPX1) mRNA expression (C) was similar in all groups. CAT mRNA expression (D) in DM was significantly lower than in healthy controls and Tempol treatment restored CAT to healthy control level. NOS2 mRNA expression (E) in DM was significantly higher than healthy controls and Tempol treatment mRNA was lower than healthy controls. In NOS3 mRNA expression (F) there was no significant change among diabetes, healthy control and Tempol treatment group.

    Article Snippet: To confirm the RT-PCR results, we performed IHC for catalase (CAT) (#ab52477, Abcam, Cambridge, MA) and iNOS (NOS2) (#53584, Anaspec, San Jose, CA).

    Techniques: Expressing

    Representative immunohistochemical staining of rat corpus cavernosum of NOS2 (iNOS). Smooth muscle and endothelium are stained brown in diabetic rats. Magnification is x100.

    Journal: International journal of impotence research

    Article Title: Superoxide dismutase analog (TEMPOL: 4-hydroxy-2, 2, 6, 6-tetramethylpiperidine 1-oxyl) treatment restores erectile function in diabetes-induced impotence

    doi: 10.1038/ijir.2009.28

    Figure Lengend Snippet: Representative immunohistochemical staining of rat corpus cavernosum of NOS2 (iNOS). Smooth muscle and endothelium are stained brown in diabetic rats. Magnification is x100.

    Article Snippet: To confirm the RT-PCR results, we performed IHC for catalase (CAT) (#ab52477, Abcam, Cambridge, MA) and iNOS (NOS2) (#53584, Anaspec, San Jose, CA).

    Techniques: Immunohistochemistry, Staining

    Immunohistochemical stain of iNOS in the hippocampus and frontal cortex of the four experimental groups. (a, b, c, d) showed the CA3 field in the hippocampus. (e, f, g, h) show the frontal cortex in the same experimental conditions.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Curcumin Reverses the Diazepam-Induced Cognitive Impairment by Modulation of Oxidative Stress and ERK 1/2/NF-κB Pathway in Brain

    doi: 10.1155/2017/3037876

    Figure Lengend Snippet: Immunohistochemical stain of iNOS in the hippocampus and frontal cortex of the four experimental groups. (a, b, c, d) showed the CA3 field in the hippocampus. (e, f, g, h) show the frontal cortex in the same experimental conditions.

    Article Snippet: Blots were then blocked and incubated with antibodies NOS2 (iNOS), NF-κ B, and phospho-NF-κ B (Ser536) (93H1) (Santa Cruz Biotechnology, Heidelberg, Germany) diluted in 1 : 500.

    Techniques: Immunohistochemistry, Staining

    The effects of curcumin administration on the expression of NF- κ B, pNF- κ B, and iNOS in the brain. Expression of NF- κ B, pNF- κ B, and iNOS was analyzed by Western blot (WB). Image analysis of Western blot bands was done by densitometry, and results were normalized to GAPDH. WB images: 1–4 hippocampus, (1 = control, 2 = CUR + CMC, 3 = DZP + CMC, and 4 = DZP + CURC + CMC) and 5–8 frontal lobe (5 = control, 6 = CUR + CMC, 7 = DZP + CMC, and 8 = DZP + CURC + CMC); ( n = 3). Each group consisted of 3 samples. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Curcumin Reverses the Diazepam-Induced Cognitive Impairment by Modulation of Oxidative Stress and ERK 1/2/NF-κB Pathway in Brain

    doi: 10.1155/2017/3037876

    Figure Lengend Snippet: The effects of curcumin administration on the expression of NF- κ B, pNF- κ B, and iNOS in the brain. Expression of NF- κ B, pNF- κ B, and iNOS was analyzed by Western blot (WB). Image analysis of Western blot bands was done by densitometry, and results were normalized to GAPDH. WB images: 1–4 hippocampus, (1 = control, 2 = CUR + CMC, 3 = DZP + CMC, and 4 = DZP + CURC + CMC) and 5–8 frontal lobe (5 = control, 6 = CUR + CMC, 7 = DZP + CMC, and 8 = DZP + CURC + CMC); ( n = 3). Each group consisted of 3 samples. ∗ p

    Article Snippet: Blots were then blocked and incubated with antibodies NOS2 (iNOS), NF-κ B, and phospho-NF-κ B (Ser536) (93H1) (Santa Cruz Biotechnology, Heidelberg, Germany) diluted in 1 : 500.

    Techniques: Expressing, Western Blot

    Autophagy inhibition lead to iNOS and p62 accumulation in Raw 264.7 and bone marrow-derived macrophages (BMDM). ( A , D ) Raw 264.7 cells ( A – C ) and BMDMs ( E – F ) were incubated with CQ (30 µM), SAR (1 µM), Torin (1 µM), Rap (1 µM) for 24h. iNOS, p62 levels were detected by western blotting. CTRL is blank control group with DMSO treatment. ( B , E ) Western blot analysis of iNOS in Raw 264.7 cells and BMDM cells under basal condition. ( C , F ) Western blot analysis of p62 in Raw 264.7 cells and BMDM cells under basal condition. ( B – F ) Values were analyzed from three individual experiments by GraphPad Prism, each experiment conducted three times. * p

    Journal: Cells

    Article Title: iNOS Interacts with Autophagy Receptor p62 and is Degraded by Autophagy in Macrophages

    doi: 10.3390/cells8101255

    Figure Lengend Snippet: Autophagy inhibition lead to iNOS and p62 accumulation in Raw 264.7 and bone marrow-derived macrophages (BMDM). ( A , D ) Raw 264.7 cells ( A – C ) and BMDMs ( E – F ) were incubated with CQ (30 µM), SAR (1 µM), Torin (1 µM), Rap (1 µM) for 24h. iNOS, p62 levels were detected by western blotting. CTRL is blank control group with DMSO treatment. ( B , E ) Western blot analysis of iNOS in Raw 264.7 cells and BMDM cells under basal condition. ( C , F ) Western blot analysis of p62 in Raw 264.7 cells and BMDM cells under basal condition. ( B – F ) Values were analyzed from three individual experiments by GraphPad Prism, each experiment conducted three times. * p

    Article Snippet: Cells then were blocked with 5% BSA (Beyotime Biotechnolog) for 2 h and stained with iNOS antibody (1:100, Santa cruz) or p62 (1:100, Abcam) antibody overnight at 4 °C.

    Techniques: Inhibition, Derivative Assay, Incubation, Western Blot

    iNOS interacts and co-localizes with p62. ( A ) Raw 264.7 cells were treated with non-vehicle or LPS for 12 h. The co-immunoprecipitation analysis of protein extracts from Raw 264.7 expressing iNOS, p62. ( B ) Raw 264.7 cells were treated with CQ (30 µM) and SAR (1 µM) for 12 h. CTRL group is blank control group with DMSO treatment. The confocal analysis showed the interaction between p62 and iNOS under CQ and SAR treatment. Raw 264.7 cells were treated with LPS (1 µg/mL) 12 h, followed by removing LPS and add CQ (30 µM), SAR (1 µM) for another 12 h. the confocal images of Raw 264.7 cells which were stained with anti-iNOS and anti-p62 were captured by Leica microsystems. The results of co-localization were analyzed by Leica Application Suite X software and GraphPad Prism.

    Journal: Cells

    Article Title: iNOS Interacts with Autophagy Receptor p62 and is Degraded by Autophagy in Macrophages

    doi: 10.3390/cells8101255

    Figure Lengend Snippet: iNOS interacts and co-localizes with p62. ( A ) Raw 264.7 cells were treated with non-vehicle or LPS for 12 h. The co-immunoprecipitation analysis of protein extracts from Raw 264.7 expressing iNOS, p62. ( B ) Raw 264.7 cells were treated with CQ (30 µM) and SAR (1 µM) for 12 h. CTRL group is blank control group with DMSO treatment. The confocal analysis showed the interaction between p62 and iNOS under CQ and SAR treatment. Raw 264.7 cells were treated with LPS (1 µg/mL) 12 h, followed by removing LPS and add CQ (30 µM), SAR (1 µM) for another 12 h. the confocal images of Raw 264.7 cells which were stained with anti-iNOS and anti-p62 were captured by Leica microsystems. The results of co-localization were analyzed by Leica Application Suite X software and GraphPad Prism.

    Article Snippet: Cells then were blocked with 5% BSA (Beyotime Biotechnolog) for 2 h and stained with iNOS antibody (1:100, Santa cruz) or p62 (1:100, Abcam) antibody overnight at 4 °C.

    Techniques: Immunoprecipitation, Expressing, Staining, Software

    iNOS level is regulated by autophagy during LPS-stimulated condition. ( A ) Raw 264.7 cells were treated with LPS (200 ng/mL) for 12 h first, followed by removing the LPS and added CQ (30 µM), SAR (1 µM), Torin (1 µM), Rap (1 µM) for another 12 h. CTRL is blank control group with DMSO treatment. ( B ) Western blot analysis of iNOS in Raw 264.7 cells under LPS treatment. ( C ) Western blot analysis of p62 in Raw 264.7 cells under LPS treatment. ( D ) Raw 264.7 cells were treated LPS (200 ng/mL) for 12 h, then removed LPS added CHX (1 µg/mL) and autophagy related drugs for another 12 h. ( E ) Level of iNOS in Raw 264.7 cell after CHX treatment. ( B – E ) Values were analyzed from three individual experiments by GraphPad Prism, each experiment conducted three times. * p

    Journal: Cells

    Article Title: iNOS Interacts with Autophagy Receptor p62 and is Degraded by Autophagy in Macrophages

    doi: 10.3390/cells8101255

    Figure Lengend Snippet: iNOS level is regulated by autophagy during LPS-stimulated condition. ( A ) Raw 264.7 cells were treated with LPS (200 ng/mL) for 12 h first, followed by removing the LPS and added CQ (30 µM), SAR (1 µM), Torin (1 µM), Rap (1 µM) for another 12 h. CTRL is blank control group with DMSO treatment. ( B ) Western blot analysis of iNOS in Raw 264.7 cells under LPS treatment. ( C ) Western blot analysis of p62 in Raw 264.7 cells under LPS treatment. ( D ) Raw 264.7 cells were treated LPS (200 ng/mL) for 12 h, then removed LPS added CHX (1 µg/mL) and autophagy related drugs for another 12 h. ( E ) Level of iNOS in Raw 264.7 cell after CHX treatment. ( B – E ) Values were analyzed from three individual experiments by GraphPad Prism, each experiment conducted three times. * p

    Article Snippet: Cells then were blocked with 5% BSA (Beyotime Biotechnolog) for 2 h and stained with iNOS antibody (1:100, Santa cruz) or p62 (1:100, Abcam) antibody overnight at 4 °C.

    Techniques: Western Blot

    Immunohistochemical stain of iNOS in the hippocampus and frontal cortex of the four experimental groups. (a, b, c, d) showed the CA3 field in the hippocampus. (e, f, g, h) show the frontal cortex in the same experimental conditions.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Curcumin Reverses the Diazepam-Induced Cognitive Impairment by Modulation of Oxidative Stress and ERK 1/2/NF-κB Pathway in Brain

    doi: 10.1155/2017/3037876

    Figure Lengend Snippet: Immunohistochemical stain of iNOS in the hippocampus and frontal cortex of the four experimental groups. (a, b, c, d) showed the CA3 field in the hippocampus. (e, f, g, h) show the frontal cortex in the same experimental conditions.

    Article Snippet: ELISA tests for total p44/42 MAP kinase (extracellular signal-regulated kinase (ERK) 1/2) were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA), and antibodies against NF-κ B, pNF-κ B, and NOS2 (iNOS) and secondary HRP-linked antibodies were from Santa Cruz Biotechnology, Heidelberg, Germany.

    Techniques: Immunohistochemistry, Staining

    The effects of curcumin administration on the expression of NF- κ B, pNF- κ B, and iNOS in the brain. Expression of NF- κ B, pNF- κ B, and iNOS was analyzed by Western blot (WB). Image analysis of Western blot bands was done by densitometry, and results were normalized to GAPDH. WB images: 1–4 hippocampus, (1 = control, 2 = CUR + CMC, 3 = DZP + CMC, and 4 = DZP + CURC + CMC) and 5–8 frontal lobe (5 = control, 6 = CUR + CMC, 7 = DZP + CMC, and 8 = DZP + CURC + CMC); ( n = 3). Each group consisted of 3 samples. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Curcumin Reverses the Diazepam-Induced Cognitive Impairment by Modulation of Oxidative Stress and ERK 1/2/NF-κB Pathway in Brain

    doi: 10.1155/2017/3037876

    Figure Lengend Snippet: The effects of curcumin administration on the expression of NF- κ B, pNF- κ B, and iNOS in the brain. Expression of NF- κ B, pNF- κ B, and iNOS was analyzed by Western blot (WB). Image analysis of Western blot bands was done by densitometry, and results were normalized to GAPDH. WB images: 1–4 hippocampus, (1 = control, 2 = CUR + CMC, 3 = DZP + CMC, and 4 = DZP + CURC + CMC) and 5–8 frontal lobe (5 = control, 6 = CUR + CMC, 7 = DZP + CMC, and 8 = DZP + CURC + CMC); ( n = 3). Each group consisted of 3 samples. ∗ p

    Article Snippet: ELISA tests for total p44/42 MAP kinase (extracellular signal-regulated kinase (ERK) 1/2) were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA), and antibodies against NF-κ B, pNF-κ B, and NOS2 (iNOS) and secondary HRP-linked antibodies were from Santa Cruz Biotechnology, Heidelberg, Germany.

    Techniques: Expressing, Western Blot

    A/ Growth rate and survival of RAW 264.7 cells cultured in medium, or medium supplemented with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells were cultured (4 × 10 6 cells in 20 ml) in medium (• œ) or medium supplemented with either IFN-γ (50 U/ml -.), or LPS (5 μg/ml -) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml -). The number of live cells recovered at the indicated times was estimated using trypan blue exclusion. Similar results were obtained estimating the cell survival either by 3H-thymidine incorporation, or by the MTT reduction test. B/ NOS2 (iNOs) mRNA induction in RAW 264.7 cultured with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells seeded into six-well plates (2.5 × 10 6 cells/well) were treated with the different stimuli as indicated above for the indicated times and analyzed by RT-PCR with specific primers for murine NOS2 (iNOs) (Table 1 ). HPRT was used as internal control for semi-quantitative estimation. C/ NOS2 (iNOs) protein in RAW 264.7 cultured with LPS or IFN-ã or LPS+IFN-γ. RAW 264.7 cells were treated with the different stimuli as indicated above for various times and cell pellets (1 × 10 6 cells) were lysed with lysis buffer. Protein concentrations in samples were adjusted and electrophoresed on 15% SDS-polyacrylamide gel, then transferred to nitro-cellulose membrane and Western bloted using polyclonal rabbit anti-murine NOS2 (iNOs), as described in Materials and Methods.

    Journal: BMC Immunology

    Article Title: Auto-protective redox buffering systems in stimulated macrophages

    doi: 10.1186/1471-2172-3-3

    Figure Lengend Snippet: A/ Growth rate and survival of RAW 264.7 cells cultured in medium, or medium supplemented with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells were cultured (4 × 10 6 cells in 20 ml) in medium (• œ) or medium supplemented with either IFN-γ (50 U/ml -.), or LPS (5 μg/ml -) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml -). The number of live cells recovered at the indicated times was estimated using trypan blue exclusion. Similar results were obtained estimating the cell survival either by 3H-thymidine incorporation, or by the MTT reduction test. B/ NOS2 (iNOs) mRNA induction in RAW 264.7 cultured with LPS or IFN-γ or LPS+IFN-γ. RAW 264.7 cells seeded into six-well plates (2.5 × 10 6 cells/well) were treated with the different stimuli as indicated above for the indicated times and analyzed by RT-PCR with specific primers for murine NOS2 (iNOs) (Table 1 ). HPRT was used as internal control for semi-quantitative estimation. C/ NOS2 (iNOs) protein in RAW 264.7 cultured with LPS or IFN-ã or LPS+IFN-γ. RAW 264.7 cells were treated with the different stimuli as indicated above for various times and cell pellets (1 × 10 6 cells) were lysed with lysis buffer. Protein concentrations in samples were adjusted and electrophoresed on 15% SDS-polyacrylamide gel, then transferred to nitro-cellulose membrane and Western bloted using polyclonal rabbit anti-murine NOS2 (iNOs), as described in Materials and Methods.

    Article Snippet: Materials The monoclonal antibody (MoAb) against NOs2 (iNOs) was purchased from Pharmingen (San Diego, CA).

    Techniques: Cell Culture, MTT Assay, Reverse Transcription Polymerase Chain Reaction, Lysis, Western Blot

    PDE5 and iNOS expression in human endothelial cells. A : PDE5 mRNA expression in HUVEC and hfCC cells: RT-PCR shows that HUVEC express detectable levels of PDE-5 mRNA. Actin was used as control. B : Western Blot analysis for PDE5 protein expression. C : Real time PCR shows that insulin (30 min) and/or sildenafil (5 h) treatments induce PDE5 and eNOS but not iNOS expression * p

    Journal: PLoS ONE

    Article Title: Sildenafil Reduces Insulin-Resistance in Human Endothelial Cells

    doi: 10.1371/journal.pone.0014542

    Figure Lengend Snippet: PDE5 and iNOS expression in human endothelial cells. A : PDE5 mRNA expression in HUVEC and hfCC cells: RT-PCR shows that HUVEC express detectable levels of PDE-5 mRNA. Actin was used as control. B : Western Blot analysis for PDE5 protein expression. C : Real time PCR shows that insulin (30 min) and/or sildenafil (5 h) treatments induce PDE5 and eNOS but not iNOS expression * p

    Article Snippet: Phospho-Akt-1 (Ser-473 and Thr-308), Akt-1, phospho eNOS (Ser-1177), eNOS and iNOS antibodies were obtained from Cell Signaling (Waltham, Mass).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Real-time Polymerase Chain Reaction

    Pam2CSK4 increases NOS1 , NOS2 , and NOS3 gene expression and NOS2 protein synthesis in odontoblast-like cells. (A) Analysis of NOS1 , NOS2 , and NOS3 gene expression after cell challenge with 10 μg/mL Pam2CSK4 for the indicated times. The three genes were significantly up-regulated upon Pam2CSK4 stimulation, NOS2 being the most up-regulated one ( n = 4). * p

    Journal: Frontiers in Physiology

    Article Title: Human odontoblast-like cells produce nitric oxide with antibacterial activity upon TLR2 activation

    doi: 10.3389/fphys.2015.00185

    Figure Lengend Snippet: Pam2CSK4 increases NOS1 , NOS2 , and NOS3 gene expression and NOS2 protein synthesis in odontoblast-like cells. (A) Analysis of NOS1 , NOS2 , and NOS3 gene expression after cell challenge with 10 μg/mL Pam2CSK4 for the indicated times. The three genes were significantly up-regulated upon Pam2CSK4 stimulation, NOS2 being the most up-regulated one ( n = 4). * p

    Article Snippet: Membranes were probed with anti-NOS1 (diluted 1:1000), anti-NOS2 (1:400), anti-NOS3 (1:1000) or anti-GAPDH (1:2000) and incubated with HRP- or alkaline phosphatase-conjugated anti-rabbit or anti-mouse IgGs (1:1000; Cell Signaling).

    Techniques: Expressing

    NOS2 transcript and protein are up-regulated in inflamed pulps from decayed teeth compared to healthy ones. (A) Analysis of NOS2 gene expression in healthy and inflamed pulps with real-time RT-PCR ( n = 3). * p

    Journal: Frontiers in Physiology

    Article Title: Human odontoblast-like cells produce nitric oxide with antibacterial activity upon TLR2 activation

    doi: 10.3389/fphys.2015.00185

    Figure Lengend Snippet: NOS2 transcript and protein are up-regulated in inflamed pulps from decayed teeth compared to healthy ones. (A) Analysis of NOS2 gene expression in healthy and inflamed pulps with real-time RT-PCR ( n = 3). * p

    Article Snippet: Membranes were probed with anti-NOS1 (diluted 1:1000), anti-NOS2 (1:400), anti-NOS3 (1:1000) or anti-GAPDH (1:2000) and incubated with HRP- or alkaline phosphatase-conjugated anti-rabbit or anti-mouse IgGs (1:1000; Cell Signaling).

    Techniques: Expressing, Quantitative RT-PCR

    MH suppressed LPS-induced iNOS and COX-2 expressions in RAW264.7 macrophage cells. (A) RAW264.7 cells were pretreated with various indicated concentrations of MH for 1 hr, then incubated with LPS (200 ng/ml) for 24 hrs. The cell lysates were prepared and subjected to Western blotting analysis by using antibodies specific for iNOS and COX-2 as described in the methods. (B) The relative protein levels were quantified by scanning densitometry and normalized to β-actin. The data indicated that MH significantly decreased the over-expression of iNOS and COX-2 induced by LPS in a concentration-dependent manner. The images shown are representatives of three independent experiments that showed consistent results and the relative protein values are expressed as mean ± S.D. for three experiments. * p

    Journal: Biomolecules & Therapeutics

    Article Title: Methyl p-Hydroxycinnamate Suppresses Lipopolysaccharide-Induced Inflammatory Responses through Akt Phosphorylation in RAW264.7 Cells

    doi: 10.4062/biomolther.2013.095

    Figure Lengend Snippet: MH suppressed LPS-induced iNOS and COX-2 expressions in RAW264.7 macrophage cells. (A) RAW264.7 cells were pretreated with various indicated concentrations of MH for 1 hr, then incubated with LPS (200 ng/ml) for 24 hrs. The cell lysates were prepared and subjected to Western blotting analysis by using antibodies specific for iNOS and COX-2 as described in the methods. (B) The relative protein levels were quantified by scanning densitometry and normalized to β-actin. The data indicated that MH significantly decreased the over-expression of iNOS and COX-2 induced by LPS in a concentration-dependent manner. The images shown are representatives of three independent experiments that showed consistent results and the relative protein values are expressed as mean ± S.D. for three experiments. * p

    Article Snippet: Specific antibodies against iNOS, COX-2, extracellular signal-regulated kinase (ERK), phosphorylated (p)-ERK, p38, p-p38, c-Jun N-terminal kinase Akt, p-Akt (1:1,000; Cell Signaling Technology), IκB-α (1:1,000; Santa Cruz Biotechnology Inc), and β-actin (1:2,500; Sigma) were diluted in 5% skim milk.

    Techniques: Incubation, Western Blot, Over Expression, Concentration Assay

    PI3K/Akt was required for MH-mediated suppression of LPS-induced iNOS and COX-2 expressions in RAW264.7 macrophage cells. RAW264.7 cells were pretreated with MH (100 μM) for 1 hr in the presence or absence of wortmannin, then exposed to LPS (200 ng/ml) for 1 hr. The cell lysates were prepared and subjected to Western blotting analysis by using antibodies specific for total and phosphorylated forms of Akt, which showed that wortmannin completely abolished MH-mediated Akt activation (A). The relative protein levels of iNOS and COX-2 were also examined. MH-mediated suppression of LPS-induced iNOS and COX-2 expressions were significantly attenuated with wortmannin but not completely (B). The images shown are representatives of three independent experiments that showed consistent results and the relative protein values are expressed as mean ± S.D. for three experiments. * p

    Journal: Biomolecules & Therapeutics

    Article Title: Methyl p-Hydroxycinnamate Suppresses Lipopolysaccharide-Induced Inflammatory Responses through Akt Phosphorylation in RAW264.7 Cells

    doi: 10.4062/biomolther.2013.095

    Figure Lengend Snippet: PI3K/Akt was required for MH-mediated suppression of LPS-induced iNOS and COX-2 expressions in RAW264.7 macrophage cells. RAW264.7 cells were pretreated with MH (100 μM) for 1 hr in the presence or absence of wortmannin, then exposed to LPS (200 ng/ml) for 1 hr. The cell lysates were prepared and subjected to Western blotting analysis by using antibodies specific for total and phosphorylated forms of Akt, which showed that wortmannin completely abolished MH-mediated Akt activation (A). The relative protein levels of iNOS and COX-2 were also examined. MH-mediated suppression of LPS-induced iNOS and COX-2 expressions were significantly attenuated with wortmannin but not completely (B). The images shown are representatives of three independent experiments that showed consistent results and the relative protein values are expressed as mean ± S.D. for three experiments. * p

    Article Snippet: Specific antibodies against iNOS, COX-2, extracellular signal-regulated kinase (ERK), phosphorylated (p)-ERK, p38, p-p38, c-Jun N-terminal kinase Akt, p-Akt (1:1,000; Cell Signaling Technology), IκB-α (1:1,000; Santa Cruz Biotechnology Inc), and β-actin (1:2,500; Sigma) were diluted in 5% skim milk.

    Techniques: Western Blot, Activation Assay

    Effect of AEGT on CCl 4 -induced acute liver inflammation in Wistar rat. Rats were given the indicated doses of AEGT for 3 days and then administered CCl 4 treatment for 1 day. Subsequently, rats were sacrificed to collect blood samples and liver sections for inflammatory parameter analysis of iNOS (A) and COX-2 (B) protein expression and secreted protein levels of proinflammatory cytokines including TNF-α (C), IL1-β (D) and IL6 (E). Values are presented as the means of five independent experiments. Error bars indicate the means ± SD of three independent experiments. * P

    Journal: PLoS ONE

    Article Title: Aqueous Extract of Gracilaria tenuistipitata Suppresses LPS-Induced NF-?B and MAPK Activation in RAW 264.7 and Rat Peritoneal Macrophages and Exerts Hepatoprotective Effects on Carbon Tetrachloride-Treated Rat

    doi: 10.1371/journal.pone.0086557

    Figure Lengend Snippet: Effect of AEGT on CCl 4 -induced acute liver inflammation in Wistar rat. Rats were given the indicated doses of AEGT for 3 days and then administered CCl 4 treatment for 1 day. Subsequently, rats were sacrificed to collect blood samples and liver sections for inflammatory parameter analysis of iNOS (A) and COX-2 (B) protein expression and secreted protein levels of proinflammatory cytokines including TNF-α (C), IL1-β (D) and IL6 (E). Values are presented as the means of five independent experiments. Error bars indicate the means ± SD of three independent experiments. * P

    Article Snippet: Anti-phosphorylated IκBα, IKKα/β, p38, JNK, ERK and anti-total iNOS, IκBα, IKKα, IKKβ, p65, p38, JNK, ERK antibodies were obtained from Cell Signaling (Beverly, MA, USA).

    Techniques: Expressing

    siRNA coupled chitosan Nps reduced levels of iNOS and apoptotic biomarkers at 24 h post-SCI. iNOS, Bcl-2 and Bax were measured in both sham animals and in mice treated with 10% Ab-siRNA-chitosan NPs. β-Tubulin served as the protein control. ***p ≤ 0.001, **p ≤ 0.01

    Journal: Journal of Nanobiotechnology

    Article Title: Targeted siRNA delivery reduces nitric oxide mediated cell death after spinal cord injury

    doi: 10.1186/s12951-017-0272-7

    Figure Lengend Snippet: siRNA coupled chitosan Nps reduced levels of iNOS and apoptotic biomarkers at 24 h post-SCI. iNOS, Bcl-2 and Bax were measured in both sham animals and in mice treated with 10% Ab-siRNA-chitosan NPs. β-Tubulin served as the protein control. ***p ≤ 0.001, **p ≤ 0.01

    Article Snippet: Immunoblotting was processed through incubating membranes with mouse specific primary antibody iNOS (MA, Cell Signaling Technology) and β-Actin Antibody (MA, Cell Signaling Technology) overnight at 4 °C.

    Techniques: Mouse Assay

    M-MDSCs derived earlier in the course of LP-BM5 infection exhibit decreased suppression of T-cell responses ). Histograms (A-E) represent the means of triplicate samples with standard deviations of a representative experiment, of a total of at least three independent experiments. The percent suppression of B-cell (A) or T-cell (C) responsiveness by M-MDSCs is depicted for both standard co-cultures and co-cultures with the addition of the iNOS inhibitor, L-NIL. The effect on suppression (blockade) by L-NIL addition on B-cell (B) versus T-cell (D) responsiveness is compared. (E) Supernates derived from parallel suppression assays in which responder cells were stimulated with anti-CD3/CD28, in the absence or presence of M-MDSCs, were assessed for nitric oxide (NO) using the Griess reagent for nitrite. *p

    Journal: Virology

    Article Title: Subpopulations of M-MDSCs from mice infected by an immunodeficiency-causing retrovirus and their differential suppression of T- vs B-cell responses

    doi: 10.1016/j.virol.2015.07.020

    Figure Lengend Snippet: M-MDSCs derived earlier in the course of LP-BM5 infection exhibit decreased suppression of T-cell responses ). Histograms (A-E) represent the means of triplicate samples with standard deviations of a representative experiment, of a total of at least three independent experiments. The percent suppression of B-cell (A) or T-cell (C) responsiveness by M-MDSCs is depicted for both standard co-cultures and co-cultures with the addition of the iNOS inhibitor, L-NIL. The effect on suppression (blockade) by L-NIL addition on B-cell (B) versus T-cell (D) responsiveness is compared. (E) Supernates derived from parallel suppression assays in which responder cells were stimulated with anti-CD3/CD28, in the absence or presence of M-MDSCs, were assessed for nitric oxide (NO) using the Griess reagent for nitrite. *p

    Article Snippet: Suppression assays were left untreated or treated with 100μM of the iNOS/NOS2 inhibitor L-NIL (Enzo Life Sciences) for the duration of the assay.

    Techniques: Derivative Assay, Infection

    AAV delivered iNOS results in NO production in a dose and time-dependent manner

    Journal: Neuroscience letters

    Article Title: A Model of Nitric Oxide Induced ?-Synuclein Misfolding in Parkinson's Disease

    doi: 10.1016/j.neulet.2012.06.070

    Figure Lengend Snippet: AAV delivered iNOS results in NO production in a dose and time-dependent manner

    Article Snippet: Briefly, for pAAV2-TRE-iNOS-WPRE construction, a PCR fragment was amplified from Nos2 (Open Biosystems) digested with Xho I – Hind III and inserted into the MCS of pAAV2-TRE-MCS-WPRE.

    Techniques:

    Effect of compounds A, B, or diclofenac (Diclo) on paw skin histology and iNOS and NF-κB expression detected by immunohistochemistry in carrageenan (CAR)-induced paw edema in mice (Original magnification 400×).

    Journal: International Journal of Molecular Sciences

    Article Title: Anti-Inflammatory and Anti-Hyperuricemic Functions of Two Synthetic Hybrid Drugs with Dual Biological Active Sites

    doi: 10.3390/ijms20225635

    Figure Lengend Snippet: Effect of compounds A, B, or diclofenac (Diclo) on paw skin histology and iNOS and NF-κB expression detected by immunohistochemistry in carrageenan (CAR)-induced paw edema in mice (Original magnification 400×).

    Article Snippet: The expression of Il1b , Il2 , Il10 , Ptgs2 (Cox-2), Ccl2 (MCP-1), Tnf , and Nos2 (iNOS) was determined using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) technique using an Applied Biosystems 7500 Instrument (Thermo Fisher Scientific, Ottawa, Ontario, Canada).

    Techniques: Expressing, Immunohistochemistry, Mouse Assay

    Effects of myeloid cell mPGES-1 deletion on macrophage abundance. ( A ) Lesion morphology analysis in aortic roots from mice on a HFD for 3 mo. Shown are representative images for CD11b ( a ), iNOS ( c ), and CD206 ( e ) staining in Mac-mPGES-1-WT aortic root

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Myeloid cell microsomal prostaglandin E synthase-1 fosters atherogenesis in mice

    doi: 10.1073/pnas.1401797111

    Figure Lengend Snippet: Effects of myeloid cell mPGES-1 deletion on macrophage abundance. ( A ) Lesion morphology analysis in aortic roots from mice on a HFD for 3 mo. Shown are representative images for CD11b ( a ), iNOS ( c ), and CD206 ( e ) staining in Mac-mPGES-1-WT aortic root

    Article Snippet: Samples were individually stained for collagen type I (1 μg/mL; 1310-01, Southern Biotech), fibronectin (0.3 μg/mL; F3648, Sigma), α-SMA (12.3μg/mL; F3777, Sigma), VCAM-1 (10 μg/mL; 553331, BD Bioscience), CD11b (5 μg/mL; 557395, BD Bioscience), CD206 (1 μg/mL; MCA2235GA, Serotech), and iNOS (0.5 μg/mL; 15323, Abcam), all with isotype-matched controls.

    Techniques: Mouse Assay, Staining

    Nitrotyrosine formation in (a) RAW 264.7 cells, (b) RAW 264.7 cells stimulated with LPS, (c) LPS-stimulated RAW 264.7 cells treated with L-NAME (25 μ M), (d) iNOS −/− RAW 264.7 cells, (e) iNOS −/− RAW 264.7 cells stimulated with LPS, and (f) RAW 264.7 cells transfected with negative control plasmid stimulated with LPS. Cells were incubated in the presence of DMEM media supplemented with 400 μ M of L-arginine.

    Journal: TheScientificWorldJournal

    Article Title: New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages

    doi: 10.1100/2011/321979

    Figure Lengend Snippet: Nitrotyrosine formation in (a) RAW 264.7 cells, (b) RAW 264.7 cells stimulated with LPS, (c) LPS-stimulated RAW 264.7 cells treated with L-NAME (25 μ M), (d) iNOS −/− RAW 264.7 cells, (e) iNOS −/− RAW 264.7 cells stimulated with LPS, and (f) RAW 264.7 cells transfected with negative control plasmid stimulated with LPS. Cells were incubated in the presence of DMEM media supplemented with 400 μ M of L-arginine.

    Article Snippet: Transfection of RAW 264.7 Cells Using an electroporation system (Gene Pulser II, Bio-Rad laboratopries, USA, for details see [ ]), cells were transfected with plasmids containing the shRNA construct, against iNOS and negative control plasmid with a scrambled sequence (Origene, USA).

    Techniques: Transfection, Negative Control, Plasmid Preparation, Incubation

    iNOS protein expression, nitrite accumulation, and O 2 • − production in RAW 264.7 and iNOS −/− RAW 264.7 cells. Macrophages were stably transfected with shRNA against iNOS and then stimulated with LPS (50 ng/mL). RAW 264.7 and iNOS −/− RAW 264.7 cells were incubated in DMEM media containing 400 μ M of L-arginine. iNOS protein expression in cell lysates, accumulation of nitrite (a), and O 2 • − production in cell supernatants (b) were determined using methods described in Section 2 ( n = 6). The O 2 • − production was also potentate using (c) PMA and (d) OZP with or without co-administration of LPS (50 ng/mL) ( n = 6). * P

    Journal: TheScientificWorldJournal

    Article Title: New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages

    doi: 10.1100/2011/321979

    Figure Lengend Snippet: iNOS protein expression, nitrite accumulation, and O 2 • − production in RAW 264.7 and iNOS −/− RAW 264.7 cells. Macrophages were stably transfected with shRNA against iNOS and then stimulated with LPS (50 ng/mL). RAW 264.7 and iNOS −/− RAW 264.7 cells were incubated in DMEM media containing 400 μ M of L-arginine. iNOS protein expression in cell lysates, accumulation of nitrite (a), and O 2 • − production in cell supernatants (b) were determined using methods described in Section 2 ( n = 6). The O 2 • − production was also potentate using (c) PMA and (d) OZP with or without co-administration of LPS (50 ng/mL) ( n = 6). * P

    Article Snippet: Transfection of RAW 264.7 Cells Using an electroporation system (Gene Pulser II, Bio-Rad laboratopries, USA, for details see [ ]), cells were transfected with plasmids containing the shRNA construct, against iNOS and negative control plasmid with a scrambled sequence (Origene, USA).

    Techniques: Expressing, Stable Transfection, Transfection, shRNA, Incubation

    Genetic depletion of HIF-1 α in astrocytes decreases expression of HIF-1 α and iNOS in astrocytes. A , Staining for HIF-1 α in GFAP-Cre:HIF-1α fl/fl and HIF-1α fl/fl mice after EAE. B , Quantification of iNOS expression in Mac-2+ macrophages and GFAP-expressing astrocytes at peak EAE in HIF-1α fl/fl mice ( n = 5). Area = 0.056 mm 2 . Data are presented as mean ± SEM from n = 5 mice. Statistical analysis was performed using an unpaired t -test with Welch’s correction ( p = 0.003 ° ). C , Quantification iNOS+ cells at peak EAE in HIF-1α fl/fl ( n =5) and GFAP-Cre:HIF-1α fl/fl ( n =4) mice. Area =0.056 mm 2 . Data are presented as mean ±SEM. Statistical analysis was performed an unpaired t test ( p = 0.034 p ). D , Double-immunofluorescence of GFAP (green) and iNOS (red) in HIF-1α fl/fl and GFAP-Cre:HIF-1α fl/fl mice at peak EAE. Statistical analysis was performed using an unpaired t test ( p = 0.01 q ). E , Quantification of iNOS+ GFAP+ cells reveals reduced iNOS-expressing astrocytes in GFAP-Cre:HIF-1α fl/fl ( n = 4) compared with HIF-1α fl/fl ( n = 5) mice. Area = 0.056 mm 2 . Statistical analysis was performed using an unpaired t test ( p = 0.0159 q ). F , Quantification of GFAP+ astrocytes shows similar numbers of astrocytes in GFAP-Cre:HIF-1α fl/fl ( n = 4) and HIF-1α fl/fl ( n = 5) mice at peak EAE. Area = 0.056 mm 2 . Data are presented as mean ± SEM. Statistical analysis was performed using an unpaired t test ( p = 0.06 r ). Superscript letters refer to the statistical results in Tables 1 , 2 , and Results.

    Journal: eNeuro

    Article Title: Hypoxia Inducible Factor-1α in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease,

    doi: 10.1523/ENEURO.0050-14.2015

    Figure Lengend Snippet: Genetic depletion of HIF-1 α in astrocytes decreases expression of HIF-1 α and iNOS in astrocytes. A , Staining for HIF-1 α in GFAP-Cre:HIF-1α fl/fl and HIF-1α fl/fl mice after EAE. B , Quantification of iNOS expression in Mac-2+ macrophages and GFAP-expressing astrocytes at peak EAE in HIF-1α fl/fl mice ( n = 5). Area = 0.056 mm 2 . Data are presented as mean ± SEM from n = 5 mice. Statistical analysis was performed using an unpaired t -test with Welch’s correction ( p = 0.003 ° ). C , Quantification iNOS+ cells at peak EAE in HIF-1α fl/fl ( n =5) and GFAP-Cre:HIF-1α fl/fl ( n =4) mice. Area =0.056 mm 2 . Data are presented as mean ±SEM. Statistical analysis was performed an unpaired t test ( p = 0.034 p ). D , Double-immunofluorescence of GFAP (green) and iNOS (red) in HIF-1α fl/fl and GFAP-Cre:HIF-1α fl/fl mice at peak EAE. Statistical analysis was performed using an unpaired t test ( p = 0.01 q ). E , Quantification of iNOS+ GFAP+ cells reveals reduced iNOS-expressing astrocytes in GFAP-Cre:HIF-1α fl/fl ( n = 4) compared with HIF-1α fl/fl ( n = 5) mice. Area = 0.056 mm 2 . Statistical analysis was performed using an unpaired t test ( p = 0.0159 q ). F , Quantification of GFAP+ astrocytes shows similar numbers of astrocytes in GFAP-Cre:HIF-1α fl/fl ( n = 4) and HIF-1α fl/fl ( n = 5) mice at peak EAE. Area = 0.056 mm 2 . Data are presented as mean ± SEM. Statistical analysis was performed using an unpaired t test ( p = 0.06 r ). Superscript letters refer to the statistical results in Tables 1 , 2 , and Results.

    Article Snippet: To detect iNOS-expressing astrocytes, we performed double-immunofluorescence staining with antibodies against iNOS (1:100, Abcam) and GFAP (1:1000, Zymed Laboratories).

    Techniques: Expressing, Staining, Mouse Assay, Immunofluorescence

    Effects of aerobic exercise on cardiac NOS2 and NOS1 expression after MI. A. Western blot analysis of cardiac NOS2 and p-NOS2 in the SC, MI, ME groups. The expression of total NOS2 and p-NOS2 remained constant in the SC and MI groups. Compared with the MI group, NOS2 was significantly activated in the ME group. B. Western blot analysis of cardiac NOS1 in the SC, MI, ME groups. The protein expression of NOS1 was significantly higher in the ME group compared with the SC and MI groups.

    Journal: PLoS ONE

    Article Title: Aerobic Exercise Inhibits Sympathetic Nerve Sprouting and Restores ?-Adrenergic Receptor Balance in Rats with Myocardial Infarction

    doi: 10.1371/journal.pone.0097810

    Figure Lengend Snippet: Effects of aerobic exercise on cardiac NOS2 and NOS1 expression after MI. A. Western blot analysis of cardiac NOS2 and p-NOS2 in the SC, MI, ME groups. The expression of total NOS2 and p-NOS2 remained constant in the SC and MI groups. Compared with the MI group, NOS2 was significantly activated in the ME group. B. Western blot analysis of cardiac NOS1 in the SC, MI, ME groups. The protein expression of NOS1 was significantly higher in the ME group compared with the SC and MI groups.

    Article Snippet: And exercise increased the expression of NOS1 and the activation of NOS2 without altering total NOS2, suggesting that exercise upregulates β3-AR expression, making β3-AR a possible source for NOS2 and NOS1 activation.

    Techniques: Expressing, Western Blot

    Representative histochemical ( a–d ) and immunohistochemical ( e,f ) staining in control ( a,c ) and injured ( b,d,e,f ) carotid arteries from C57BL/6 iNOS-null mice. Hematoxylin and eosin staining ( a,b ) indicates a cellular thickening in the injured

    Journal:

    Article Title: Apolipoprotein E Inhibition of Vascular Hyperplasia and Neointima Formation Requires Inducible Nitric Oxide Synthase

    doi: 10.1194/jlr.M500177-JLR200

    Figure Lengend Snippet: Representative histochemical ( a–d ) and immunohistochemical ( e,f ) staining in control ( a,c ) and injured ( b,d,e,f ) carotid arteries from C57BL/6 iNOS-null mice. Hematoxylin and eosin staining ( a,b ) indicates a cellular thickening in the injured

    Article Snippet: Wild type C57BL/6J and FVB/N mice, as well as the apoE −/− and the iNOS-deficient NOS2 −/− mice were obtained from the Jackson Laboratories (Bar Harbor, MI).

    Techniques: Immunohistochemistry, Staining, Mouse Assay

    Immunodetection of apoE and iNOS after vascular injury in genetically-modified mice. Immnuofluorescent analysis of injured arteries from C57BL/6 apoE − / − mice ( a,c,e ) and apoE transgenic FVB/N mice ( b,d,f ) 24 hr after endothelial denudation.

    Journal:

    Article Title: Apolipoprotein E Inhibition of Vascular Hyperplasia and Neointima Formation Requires Inducible Nitric Oxide Synthase

    doi: 10.1194/jlr.M500177-JLR200

    Figure Lengend Snippet: Immunodetection of apoE and iNOS after vascular injury in genetically-modified mice. Immnuofluorescent analysis of injured arteries from C57BL/6 apoE − / − mice ( a,c,e ) and apoE transgenic FVB/N mice ( b,d,f ) 24 hr after endothelial denudation.

    Article Snippet: Wild type C57BL/6J and FVB/N mice, as well as the apoE −/− and the iNOS-deficient NOS2 −/− mice were obtained from the Jackson Laboratories (Bar Harbor, MI).

    Techniques: Immunodetection, Genetically Modified, Mouse Assay, Transgenic Assay

    Morphometric quantification of control (open bars) and injured (filled bars) carotid arteries from C57BL/6 wild-type and iNOS-defective NOS2 − / − mice. Panel A shows neointimal area of the injury carotid arteries of C57BL/6 wild type and

    Journal:

    Article Title: Apolipoprotein E Inhibition of Vascular Hyperplasia and Neointima Formation Requires Inducible Nitric Oxide Synthase

    doi: 10.1194/jlr.M500177-JLR200

    Figure Lengend Snippet: Morphometric quantification of control (open bars) and injured (filled bars) carotid arteries from C57BL/6 wild-type and iNOS-defective NOS2 − / − mice. Panel A shows neointimal area of the injury carotid arteries of C57BL/6 wild type and

    Article Snippet: Wild type C57BL/6J and FVB/N mice, as well as the apoE −/− and the iNOS-deficient NOS2 −/− mice were obtained from the Jackson Laboratories (Bar Harbor, MI).

    Techniques: Mouse Assay

    Morphometric quantification of control ( open bars ) and injured ( filled bars ) carotid arteries from FVB/N wild type and apoE transgenic mice given daily injections of saline or the iNOS inhibitor 1400W. Panel A shows neointimal area of the injury carotid

    Journal:

    Article Title: Apolipoprotein E Inhibition of Vascular Hyperplasia and Neointima Formation Requires Inducible Nitric Oxide Synthase

    doi: 10.1194/jlr.M500177-JLR200

    Figure Lengend Snippet: Morphometric quantification of control ( open bars ) and injured ( filled bars ) carotid arteries from FVB/N wild type and apoE transgenic mice given daily injections of saline or the iNOS inhibitor 1400W. Panel A shows neointimal area of the injury carotid

    Article Snippet: Wild type C57BL/6J and FVB/N mice, as well as the apoE −/− and the iNOS-deficient NOS2 −/− mice were obtained from the Jackson Laboratories (Bar Harbor, MI).

    Techniques: Transgenic Assay, Mouse Assay

    Immunostaining of apoE and iNOS in denuded carotid arteries of inbred mice. Carotid arteries in C57BL/6 ( a,c,e ) and FVB/N ( b,d,f ) mice were injured by mechanical denudation. Tissues were harvested after 24 hr and sections were immunostained with fluorescent

    Journal:

    Article Title: Apolipoprotein E Inhibition of Vascular Hyperplasia and Neointima Formation Requires Inducible Nitric Oxide Synthase

    doi: 10.1194/jlr.M500177-JLR200

    Figure Lengend Snippet: Immunostaining of apoE and iNOS in denuded carotid arteries of inbred mice. Carotid arteries in C57BL/6 ( a,c,e ) and FVB/N ( b,d,f ) mice were injured by mechanical denudation. Tissues were harvested after 24 hr and sections were immunostained with fluorescent

    Article Snippet: Wild type C57BL/6J and FVB/N mice, as well as the apoE −/− and the iNOS-deficient NOS2 −/− mice were obtained from the Jackson Laboratories (Bar Harbor, MI).

    Techniques: Immunostaining, Mouse Assay

    Pathogenesis of M. tuberculosis in iNOS −/− , gp91 phox−/− iNOS −/− , and IFN-γ −/− mice. IFN-γ −/− mice (filled squares), iNOS −/− mice (open circles),

    Journal:

    Article Title: Identification of Mycobacterium tuberculosis Counterimmune (cim) Mutants in Immunodeficient Mice by Differential Screening

    doi: 10.1128/IAI.72.9.5315-5321.2004

    Figure Lengend Snippet: Pathogenesis of M. tuberculosis in iNOS −/− , gp91 phox−/− iNOS −/− , and IFN-γ −/− mice. IFN-γ −/− mice (filled squares), iNOS −/− mice (open circles),

    Article Snippet: Male and female C57BL/6, IFN-γ−/− , and iNOS−/− mice, 6 to 10 weeks of age, were from Jackson Laboratories. gp91phox−/− iNOS−/− mice were generated and maintained as described previously ( ).

    Techniques: Mouse Assay

    Cancer-adjacent normal tissues express a similar in vivo pattern of macrophage polarization distinguishing BBC from luminal breast cancer (A) H E stained sections for tissues adjacent to BBC or luminal breast cancer and dual stained immunofluorescent images with staining for M1/IL-7R (red) and M2/CD36 (green). (B) H E sections for the same specimens, but with dual staining for M1/iNOS (blue) and M2/CD163 (red). More dual stained M1/M2 macrophages and higher numbers of M2 positive macrophages are observed in the BBC-adjacent tissues in this set of samples.

    Journal: Molecular cancer research : MCR

    Article Title: Basal-like breast cancer cells induce phenotypic and genomic changes in macrophages

    doi: 10.1158/1541-7786.MCR-11-0604

    Figure Lengend Snippet: Cancer-adjacent normal tissues express a similar in vivo pattern of macrophage polarization distinguishing BBC from luminal breast cancer (A) H E stained sections for tissues adjacent to BBC or luminal breast cancer and dual stained immunofluorescent images with staining for M1/IL-7R (red) and M2/CD36 (green). (B) H E sections for the same specimens, but with dual staining for M1/iNOS (blue) and M2/CD163 (red). More dual stained M1/M2 macrophages and higher numbers of M2 positive macrophages are observed in the BBC-adjacent tissues in this set of samples.

    Article Snippet: Following treatment with chemicals or coculture, these coverslips were fixed and stained for the expression of M1 macrophage markers NOS2a/iNOS (C-11, Santa Cruz Biotechnology) and IL-7R (C-20, Santa Cruz Biotechnology), or M2 macrophage markers CD36 (5–271, BioLegend, Inc.) and CD163 (RM3/1, BioLegend, Inc.), according to standard protocols.

    Techniques: In Vivo, Staining

    Effects of IVIg treatment in inflammatory infiltration in peripheral nerves. a Immunohistochemistry for CD68: After 3 and 8 weeks of BTZ treatment, both sciatic and caudal nerves show a massive macrophage infiltration which is almost completely abrogated by the preventive co-treatment with IVIg (BTZ + IVIg 3; BTZ + IVIg 8). In the therapeutic setting (BTZ + IVIg 4), a milder reduction of CD68 + infiltrating cells was achieved in both sciatic and caudal nerves. Scale bar 50 μm. b Representative immunohistochemistry for iNOS and ARG1 after 3 weeks of BTZ treatment. Most infiltrating macrophages (CD68+) are iNOS + pro-inflammatory M1 type while a very limited amount of them are ARG1+ anti-inflammatory M2 type. Scale bar 50 μm

    Journal: Journal of Neuroinflammation

    Article Title: High-dose intravenous immunoglobulins reduce nerve macrophage infiltration and the severity of bortezomib-induced peripheral neurotoxicity in rats

    doi: 10.1186/s12974-018-1270-x

    Figure Lengend Snippet: Effects of IVIg treatment in inflammatory infiltration in peripheral nerves. a Immunohistochemistry for CD68: After 3 and 8 weeks of BTZ treatment, both sciatic and caudal nerves show a massive macrophage infiltration which is almost completely abrogated by the preventive co-treatment with IVIg (BTZ + IVIg 3; BTZ + IVIg 8). In the therapeutic setting (BTZ + IVIg 4), a milder reduction of CD68 + infiltrating cells was achieved in both sciatic and caudal nerves. Scale bar 50 μm. b Representative immunohistochemistry for iNOS and ARG1 after 3 weeks of BTZ treatment. Most infiltrating macrophages (CD68+) are iNOS + pro-inflammatory M1 type while a very limited amount of them are ARG1+ anti-inflammatory M2 type. Scale bar 50 μm

    Article Snippet: Immunohistochemistry was performed using anti-CD68 antibody (CD68 Biorad MCA341GA, Segrate Milan, Italy) to detect macrophage infiltrating cells, anti-iNOS antibody (Biorbyt orb13614, Cambridge, UK), and anti-ARG1 antibody (Biorbyt orb394005, Cambridge, UK) to discriminate M1 (pro-inflammatory) from M2 (anti-inflammatory) macrophages.

    Techniques: Immunohistochemistry

    IFNγ and TNFα are critical mediators of MDSC hyper-activation induced by type-1 immune effector cells. (A) Expression of IDO1, NOS2, IL10, and COX2 in OvCa ascites-isolated MDSCs cultured for 24 h with or without IL18/IFNα-activated

    Journal: Cancer immunology research

    Article Title: Synergistic COX2 induction by IFNγ and TNFα self-limits type-1 immunity in the human tumor microenvironment

    doi: 10.1158/2326-6066.CIR-15-0157

    Figure Lengend Snippet: IFNγ and TNFα are critical mediators of MDSC hyper-activation induced by type-1 immune effector cells. (A) Expression of IDO1, NOS2, IL10, and COX2 in OvCa ascites-isolated MDSCs cultured for 24 h with or without IL18/IFNα-activated

    Article Snippet: MDSCs were stained for CD11b-FITC, CD14-PE, CD33-APC, CD34-PE-Cy5, CD11c-PE, HLA-DR-PE, DC-SIGN-FITC, CD80-FITC, CD86-FITC, and CD83-PE (BD Biosciences and eBioscience), as well as IDO-A488 (R & D Systems), NOS2-PE (Santa Cruz Biotechnology), and COX1-FITC/COX2-PE (BD Biosciences).

    Techniques: Activation Assay, Expressing, Isolation, Cell Culture

    Type-1 effector cell-driven hyperactivation of MDSCs requires the intact COX2-PGE 2 axis. (A) Expression of IDO1, NOS2, IL10, COX2, IFNγ, and TNFα in OvCa ascites-isolated MDSCs cultured for 24 h with or without IL18/IFNα-activated

    Journal: Cancer immunology research

    Article Title: Synergistic COX2 induction by IFNγ and TNFα self-limits type-1 immunity in the human tumor microenvironment

    doi: 10.1158/2326-6066.CIR-15-0157

    Figure Lengend Snippet: Type-1 effector cell-driven hyperactivation of MDSCs requires the intact COX2-PGE 2 axis. (A) Expression of IDO1, NOS2, IL10, COX2, IFNγ, and TNFα in OvCa ascites-isolated MDSCs cultured for 24 h with or without IL18/IFNα-activated

    Article Snippet: MDSCs were stained for CD11b-FITC, CD14-PE, CD33-APC, CD34-PE-Cy5, CD11c-PE, HLA-DR-PE, DC-SIGN-FITC, CD80-FITC, CD86-FITC, and CD83-PE (BD Biosciences and eBioscience), as well as IDO-A488 (R & D Systems), NOS2-PE (Santa Cruz Biotechnology), and COX1-FITC/COX2-PE (BD Biosciences).

    Techniques: Expressing, Isolation, Cell Culture

    Renal nitric oxide synthase (NOS) protein abundance and urinary nitrite/nitrate concentration. 5A . Immunoblots of NOS1, NOS2, and NOS3 in whole kidney homogenates (n=5/group). 5B . Densitometric analysis of the immunoblots. On NS (0.8% NaCl) diet, the renal protein abundances of NOS1 and NOS2 in GRK4γ486V (486V) transgenic mice and Non-transgenic (Non-T) littermates are similar and not altered by HS (6% NaCl) diet. NOS3 protein abundance is similar in 486V and Non-T mice on NS diet but is decreased by HS diet in 486V (49±2%) but not Non-T mice. *P

    Journal: Free radical biology & medicine

    Article Title: INCREASED RENAL OXIDATIVE STRESS IN SALT-SENSITIVE HUMAN GRK4γ486V TRANSGENIC MICE

    doi: 10.1016/j.freeradbiomed.2017.02.021

    Figure Lengend Snippet: Renal nitric oxide synthase (NOS) protein abundance and urinary nitrite/nitrate concentration. 5A . Immunoblots of NOS1, NOS2, and NOS3 in whole kidney homogenates (n=5/group). 5B . Densitometric analysis of the immunoblots. On NS (0.8% NaCl) diet, the renal protein abundances of NOS1 and NOS2 in GRK4γ486V (486V) transgenic mice and Non-transgenic (Non-T) littermates are similar and not altered by HS (6% NaCl) diet. NOS3 protein abundance is similar in 486V and Non-T mice on NS diet but is decreased by HS diet in 486V (49±2%) but not Non-T mice. *P

    Article Snippet: The polyclonal antibodies against the proteins of interest were: NOX1 (SC5821, Santa Cruz, Dallas, Texas); NOX2 (07–024, Upstate, Billerica, MA); CuZnSOD, MnSOD (SOD101, SOD111, Stressgen, Ann Arbor, MI); ECSOD, nitrotyrosine (Ab83108, 42789, Abcam, Cambridge, MA); NOS1/NOS2 (SC648, SC651, Santa Cruz); NOS3 (610296, BD Biosciences, San Jose, CA); HO-1(SC10789, Santa Cruz); HO-2 (ADI-OSA-200, Enzo, Farmingdale, NY), and actin (A2066, Sigma, St. Louis, MO).

    Techniques: Concentration Assay, Western Blot, Transgenic Assay, Mouse Assay

    Differences in localization patterns of Actin, Tubulin, CD11b and E-Selectin in APECs from C57BL/6 and Nos2 -/- APECs upon infection. Fluorescence microscopic images of F-Actin (green) and α-Tubulin (red) in APECs from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (A). Yellow and white arrows represent Actin and Tubulin localization to the cortex, respectively. Confocal microscopic images of E-Selectin (green) and CD11b (red) in APECs taken at 100X magnification from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (B). The white arrows depict CD11b localization at the cell-cell interface in C57BL/6 APECs from infected mice. On the other hand, APECs from infected Nos2 -/- mice display diffused CD11b pattern. Scale bar for fluorescent microscopic images is 10 μm. The data is presented as mean ± S.E from two independent representative experiments with three mice per group.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: Differences in localization patterns of Actin, Tubulin, CD11b and E-Selectin in APECs from C57BL/6 and Nos2 -/- APECs upon infection. Fluorescence microscopic images of F-Actin (green) and α-Tubulin (red) in APECs from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (A). Yellow and white arrows represent Actin and Tubulin localization to the cortex, respectively. Confocal microscopic images of E-Selectin (green) and CD11b (red) in APECs taken at 100X magnification from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice four days post infection, left untreated ex vivo for 24 h (B). The white arrows depict CD11b localization at the cell-cell interface in C57BL/6 APECs from infected mice. On the other hand, APECs from infected Nos2 -/- mice display diffused CD11b pattern. Scale bar for fluorescent microscopic images is 10 μm. The data is presented as mean ± S.E from two independent representative experiments with three mice per group.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques: Infection, Fluorescence, Mouse Assay, Ex Vivo

    Nos2 mediates aggregation of APECs upon S . Typhimurium infection of mice. The amounts of Tnfα, Il6 and Ifnγ (A) in the sera from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice at day two and four post infection. The amounts of nitrite (B) and cell aggregates (C) of APECs isolated from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice at indicated days post infection and cultured ex vivo for 24 h. Representative bright field microscopic images of APECs, either at 40X (Leica DMI6000B) or 63X (Leica TCS SP5), with the scale bar representing 20 μm, of APECs from uninfected or infected mice are shown (D). The data is representative of at least four independent experiments with a minimum of three mice per condition. Significance with respect to untreated C57BL/6 controls, untreated Nos2 -/- controls, S . Typhimurium infected C57BL/6 mice day 2 control and S . Typhimurium infected C57BL/6 mice day 4 control are represented as *, θ, τ, and # respectively.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: Nos2 mediates aggregation of APECs upon S . Typhimurium infection of mice. The amounts of Tnfα, Il6 and Ifnγ (A) in the sera from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice at day two and four post infection. The amounts of nitrite (B) and cell aggregates (C) of APECs isolated from uninfected and S . Typhimurium infected C57BL/6 and Nos2 -/- mice at indicated days post infection and cultured ex vivo for 24 h. Representative bright field microscopic images of APECs, either at 40X (Leica DMI6000B) or 63X (Leica TCS SP5), with the scale bar representing 20 μm, of APECs from uninfected or infected mice are shown (D). The data is representative of at least four independent experiments with a minimum of three mice per condition. Significance with respect to untreated C57BL/6 controls, untreated Nos2 -/- controls, S . Typhimurium infected C57BL/6 mice day 2 control and S . Typhimurium infected C57BL/6 mice day 4 control are represented as *, θ, τ, and # respectively.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques: Infection, Mouse Assay, Isolation, Cell Culture, Ex Vivo

    Nos2 regulates the morphology of APECs treated with Ifnγ. Representative bright field images acquired at a magnification of 60X (A) and ESEM images acquired at a magnification of 5000X (B) of C57BL/6 and Nos2 -/- APECs treated with 25 U/ml of Ifnγ for 36 h is shown. In addition, the images of Nos2 -/- APECs cultured in the absence or presence of 100 μM of SNAP treated in absence or presence of 25 U/ml of Ifnγ for 36 h is shown.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: Nos2 regulates the morphology of APECs treated with Ifnγ. Representative bright field images acquired at a magnification of 60X (A) and ESEM images acquired at a magnification of 5000X (B) of C57BL/6 and Nos2 -/- APECs treated with 25 U/ml of Ifnγ for 36 h is shown. In addition, the images of Nos2 -/- APECs cultured in the absence or presence of 100 μM of SNAP treated in absence or presence of 25 U/ml of Ifnγ for 36 h is shown.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques: Cell Culture

    Nos2 derived NO mediates the Ifnγ induced aggregation of APECs. Kinetic analysis of amounts of ROS (A), nitrite (D) of APECs treated with 25 U/ml of Ifnγ. The amounts of ROS (B) and the number of cell aggregates (C) in APECs treated with Ifnγ in the presence or absence of indicated doses of PEG-Catalase (PC) for 36 h. The amount of nitrite (E) and the number of cell aggregates (F) of APECs treated with Ifnγ in the presence or absence of indicated doses of LNMA for 36 h. Kinetic analysis of the number of viable APECs from C57BL/6 and Nos2 -/- mice left untreated or upon treatment with 25 U/ml of Ifnγ (G). The amount of nitrite (H) and the number of cell aggregates (I) from Ifnγ treated Nos2 -/- APECs in the absence or presence of indicated doses of SNAP for 36 h in comparison to C57BL/6 APECs treated with 25 U/ml Ifnγ for 36 h. The data is represented as mean ± S.E from three independent experiments. The significance with respect to untreated controls, Ifnγ treated C57BL/6 APECs controls and untreated Nos2 -/- APECs controls are represented as *, θ and Δ respectively.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: Nos2 derived NO mediates the Ifnγ induced aggregation of APECs. Kinetic analysis of amounts of ROS (A), nitrite (D) of APECs treated with 25 U/ml of Ifnγ. The amounts of ROS (B) and the number of cell aggregates (C) in APECs treated with Ifnγ in the presence or absence of indicated doses of PEG-Catalase (PC) for 36 h. The amount of nitrite (E) and the number of cell aggregates (F) of APECs treated with Ifnγ in the presence or absence of indicated doses of LNMA for 36 h. Kinetic analysis of the number of viable APECs from C57BL/6 and Nos2 -/- mice left untreated or upon treatment with 25 U/ml of Ifnγ (G). The amount of nitrite (H) and the number of cell aggregates (I) from Ifnγ treated Nos2 -/- APECs in the absence or presence of indicated doses of SNAP for 36 h in comparison to C57BL/6 APECs treated with 25 U/ml Ifnγ for 36 h. The data is represented as mean ± S.E from three independent experiments. The significance with respect to untreated controls, Ifnγ treated C57BL/6 APECs controls and untreated Nos2 -/- APECs controls are represented as *, θ and Δ respectively.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques: Derivative Assay, Mouse Assay

    Nos2 regulates the cell surface amounts and relocalization of CD11b in response to Ifnγ in APECs. Comparative analysis of cell surface amounts of MHC class II (A), Lfa1 (B), E-Selectin (C) and CD11b (D) on APECs from C57BL/6 and Nos2 -/- mice at 36 h post 25 U/ml of Ifnγ treatment. The data is represented as mean ± S.E from three independent experiments. Fluorescence microscopic images with a scale of 10 μm of E-Selectin (E) and CD11b (F) on APECs from C57BL/6 and Nos2 -/- mice at 36 h post 25 U/ml of Ifnγ treatment. The significance with respect to untreated C57BL/6 APECs controls and untreated Nos2 -/- APECs controls are represented as * and Δ respectively.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: Nos2 regulates the cell surface amounts and relocalization of CD11b in response to Ifnγ in APECs. Comparative analysis of cell surface amounts of MHC class II (A), Lfa1 (B), E-Selectin (C) and CD11b (D) on APECs from C57BL/6 and Nos2 -/- mice at 36 h post 25 U/ml of Ifnγ treatment. The data is represented as mean ± S.E from three independent experiments. Fluorescence microscopic images with a scale of 10 μm of E-Selectin (E) and CD11b (F) on APECs from C57BL/6 and Nos2 -/- mice at 36 h post 25 U/ml of Ifnγ treatment. The significance with respect to untreated C57BL/6 APECs controls and untreated Nos2 -/- APECs controls are represented as * and Δ respectively.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques: Mouse Assay, Fluorescence

    A schematic model representing the aggregation response of APECs in response to Ifnγ treatment. In the presence of Ifnγ, Nos2 is induced resulting in high amounts of NO. Both NO and additional Ifnγ specific signals are required for stability and cortical arrangement of Actin and Tubulin, which affects several cellular functions, including motility, phagocytosis and maintenance of morphology. In addition, re-localization of CD11b enhances the aggregation of APECs which are more efficient in reducing the number of intracellular bacteria and increasing host resistance.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: A schematic model representing the aggregation response of APECs in response to Ifnγ treatment. In the presence of Ifnγ, Nos2 is induced resulting in high amounts of NO. Both NO and additional Ifnγ specific signals are required for stability and cortical arrangement of Actin and Tubulin, which affects several cellular functions, including motility, phagocytosis and maintenance of morphology. In addition, re-localization of CD11b enhances the aggregation of APECs which are more efficient in reducing the number of intracellular bacteria and increasing host resistance.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques:

    Actin and Tubulin stabilization contribute to Ifnγ induced aggregation of APECs. Fluorescence microscopic images acquired at a magnification of 63X with the scale bar representing 10 μm of F-Actin (green) and α-Tubulin (red) in C57BL/6 APECs treated with 10 μM Cyt D and 1 μg/ml of Col respectively for 6 h (A). Yellow and white arrows indicate cortical arrangement of F-Actin and α-Tubulin respectively. Thin white arrows indicate the cell boundary while the fatter white arrows are used to highlight the shrinkage of the α-Tubulin network upon Col treatment when compared to the untreated control C57BL/6 APECs. The amount of nitrite in the supernatant (B) produced by APECs from C57BL/6 mice upon treatment with 25 U/ml of Ifnγ in the absence or presence indicated doses of inhibitors Cyt D (μM) and Col (μg/ml). The number of cell aggregates (C) of APECs from C57BL/6 mice upon treatment with 25 U/ml of Ifnγ in the absence or presence of indicated doses of inhibitors Cyt D (μM) and Col (μg/ml). The amounts of E-Selectin (D) and CD11b (E) on the cell surface of C57BL/6 APECs treated with 25 U/ml of Ifnγ for 36 h, post 6 h of pretreatment without or with Cyt D (10 μM) and Col (1 μg/ml). The data is represented as mean ± S.E from three independent experiments. The velocity of APECs from C57BL/6 and Nos2 -/- mice treated without or with 25 U/ml of Ifnγ between 18–24 h of addition (F). The velocity of APECs from C57BL/6 pretreated for 6 h with Cyt D (10 μM) and Col (1 μg/ml) before tracking them from 6–12 h post treatment. Also, the velocity of Nos2 -/- APECs pretreated in the presence of 5 μM of SNAP (SNAP lo ) and 100 μM of SNAP (SNAP hi ) for 12 h and without or with 25 U/ml of Ifnγ before tracking cells 18–24 h post Ifnγ addition. The velocity for each of the above mentioned conditions are represented as percentage velocity with respect to average velocity exhibited by untreated C57BL/6 APECs. The data is representative of two independent experiments. Significance is represented as * when compared to untreated controls and # when compared to Ifnγ treated C57BL/6 APECs respectively. The significance with respect to untreated controls, Ifnγ treated C57BL/6 APECs controls. untreated Nos2 -/- and SNAP lo alone Nos2 -/- APECs controls are represented as *, θ, Δ, and # respectively.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: Actin and Tubulin stabilization contribute to Ifnγ induced aggregation of APECs. Fluorescence microscopic images acquired at a magnification of 63X with the scale bar representing 10 μm of F-Actin (green) and α-Tubulin (red) in C57BL/6 APECs treated with 10 μM Cyt D and 1 μg/ml of Col respectively for 6 h (A). Yellow and white arrows indicate cortical arrangement of F-Actin and α-Tubulin respectively. Thin white arrows indicate the cell boundary while the fatter white arrows are used to highlight the shrinkage of the α-Tubulin network upon Col treatment when compared to the untreated control C57BL/6 APECs. The amount of nitrite in the supernatant (B) produced by APECs from C57BL/6 mice upon treatment with 25 U/ml of Ifnγ in the absence or presence indicated doses of inhibitors Cyt D (μM) and Col (μg/ml). The number of cell aggregates (C) of APECs from C57BL/6 mice upon treatment with 25 U/ml of Ifnγ in the absence or presence of indicated doses of inhibitors Cyt D (μM) and Col (μg/ml). The amounts of E-Selectin (D) and CD11b (E) on the cell surface of C57BL/6 APECs treated with 25 U/ml of Ifnγ for 36 h, post 6 h of pretreatment without or with Cyt D (10 μM) and Col (1 μg/ml). The data is represented as mean ± S.E from three independent experiments. The velocity of APECs from C57BL/6 and Nos2 -/- mice treated without or with 25 U/ml of Ifnγ between 18–24 h of addition (F). The velocity of APECs from C57BL/6 pretreated for 6 h with Cyt D (10 μM) and Col (1 μg/ml) before tracking them from 6–12 h post treatment. Also, the velocity of Nos2 -/- APECs pretreated in the presence of 5 μM of SNAP (SNAP lo ) and 100 μM of SNAP (SNAP hi ) for 12 h and without or with 25 U/ml of Ifnγ before tracking cells 18–24 h post Ifnγ addition. The velocity for each of the above mentioned conditions are represented as percentage velocity with respect to average velocity exhibited by untreated C57BL/6 APECs. The data is representative of two independent experiments. Significance is represented as * when compared to untreated controls and # when compared to Ifnγ treated C57BL/6 APECs respectively. The significance with respect to untreated controls, Ifnγ treated C57BL/6 APECs controls. untreated Nos2 -/- and SNAP lo alone Nos2 -/- APECs controls are represented as *, θ, Δ, and # respectively.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques: Fluorescence, Produced, Mouse Assay

    The cortical stability of cytoskeleton elements, Actin and Tubulin, is regulated in a Nos2 dependent manner upon Ifnγ treatment. Fluorescence microscopic images, with the scale bar of 10 μm, of F-Actin (green) and α-Tubulin (red) in APECs from C57BL/6 mice and Nos2 -/- mice treated without or with 25 U/ml of Ifnγ for 36 h. In addition, Nos2 -/- APECs were treated with 100 μM of SNAP in the absence or presence of Ifnγ. The white arrows indicate cortical arrangement of F-Actin and α-Tubulin.

    Journal: PLoS ONE

    Article Title: Interferon-Gamma and Nitric Oxide Synthase 2 Mediate the Aggregation of Resident Adherent Peritoneal Exudate Cells: Implications for the Host Response to Pathogens

    doi: 10.1371/journal.pone.0128301

    Figure Lengend Snippet: The cortical stability of cytoskeleton elements, Actin and Tubulin, is regulated in a Nos2 dependent manner upon Ifnγ treatment. Fluorescence microscopic images, with the scale bar of 10 μm, of F-Actin (green) and α-Tubulin (red) in APECs from C57BL/6 mice and Nos2 -/- mice treated without or with 25 U/ml of Ifnγ for 36 h. In addition, Nos2 -/- APECs were treated with 100 μM of SNAP in the absence or presence of Ifnγ. The white arrows indicate cortical arrangement of F-Actin and α-Tubulin.

    Article Snippet: Absence of Nos2 does not skew macrophage polarization signals in response to Ifnγ Ifnγ in known to polarize macrophages to the M1 phenotype and the induction of Nos2 is an important marker [ ].

    Techniques: Fluorescence, Mouse Assay

    A schematic representation of the relaxin pathway when disturbed by simazine. Simazine inhibits testicular Rxfp1 expression, which consequently may limit the intracellular signaling of relaxin in the testis. The compromised relaxin-Rxfp1 signaling is further expected to downregulate the expression of relaxin target genes such as Nos2, Nos3, Vegf, and Mmp9, leading to the inhibition of NO release.

    Journal: PLoS ONE

    Article Title: Disturbed Relaxin Signaling Pathway and Testicular Dysfunction in Mouse Offspring upon Maternal Exposure to Simazine

    doi: 10.1371/journal.pone.0044856

    Figure Lengend Snippet: A schematic representation of the relaxin pathway when disturbed by simazine. Simazine inhibits testicular Rxfp1 expression, which consequently may limit the intracellular signaling of relaxin in the testis. The compromised relaxin-Rxfp1 signaling is further expected to downregulate the expression of relaxin target genes such as Nos2, Nos3, Vegf, and Mmp9, leading to the inhibition of NO release.

    Article Snippet: Simazine-induced Decreased Rxfp1 Expression and NO Production in Leydig Cells in vitro The binding of relaxin to Rxfp1 increases transcription of its target genes, including Nos2, leading to stimulated production of NO , .

    Techniques: Expressing, Inhibition

    Simazine-induced decreased Rxfp1 and Nos2 expression and reduced NO production in testicular cells in vitro . Rat Leydig cells (LC540) were exposed to simazine (0, 0.01, 0.1, or 1 µM) for 36 h, and the mRNA levels of Rxfp1 (A) and Nos2 (B) were analyzed by qRT-PCR. The relative expression levels of Rxfp1 and Nos2 normalized to β-actin are shown. (C) LC540 cells were exposed to various concentrations of simazine for 36 h, and the levels of NO produced were determined by measuring nitrite concentration. (D) LC540 knockdown cells were prepared by transfection with a scrambled sequence or siRNAs for Rxfp1, and reduced Rxfp1 expression was demonstrated by western blotting. Subsequently, knocked-down cells were treated with simazine for 36 h, and the levels of NO produced were determined. For all experiments (A-D), three independent experiments were performed in triplicate, and different letters denote significant values ( p

    Journal: PLoS ONE

    Article Title: Disturbed Relaxin Signaling Pathway and Testicular Dysfunction in Mouse Offspring upon Maternal Exposure to Simazine

    doi: 10.1371/journal.pone.0044856

    Figure Lengend Snippet: Simazine-induced decreased Rxfp1 and Nos2 expression and reduced NO production in testicular cells in vitro . Rat Leydig cells (LC540) were exposed to simazine (0, 0.01, 0.1, or 1 µM) for 36 h, and the mRNA levels of Rxfp1 (A) and Nos2 (B) were analyzed by qRT-PCR. The relative expression levels of Rxfp1 and Nos2 normalized to β-actin are shown. (C) LC540 cells were exposed to various concentrations of simazine for 36 h, and the levels of NO produced were determined by measuring nitrite concentration. (D) LC540 knockdown cells were prepared by transfection with a scrambled sequence or siRNAs for Rxfp1, and reduced Rxfp1 expression was demonstrated by western blotting. Subsequently, knocked-down cells were treated with simazine for 36 h, and the levels of NO produced were determined. For all experiments (A-D), three independent experiments were performed in triplicate, and different letters denote significant values ( p

    Article Snippet: Simazine-induced Decreased Rxfp1 Expression and NO Production in Leydig Cells in vitro The binding of relaxin to Rxfp1 increases transcription of its target genes, including Nos2, leading to stimulated production of NO , .

    Techniques: Expressing, In Vitro, Quantitative RT-PCR, Produced, Concentration Assay, Transfection, Sequencing, Western Blot

    Reduced expression of target genes in the relaxin pathway in the testis of F1 mice exposed to simazine. (A) The expression of crucial target genes (Nos2, Nos3, Vegf, Mmp9) in the relaxin pathway was determined by qRT-PCR on the testis of young adult F1 control or 500 µg/kg simazine-exposed mice. The data (mean ± SEM) are from six testicular analyses performed in triplicate for each group and are shown as relative fold changes. Asterisks indicate significant differences compared with the control (* p

    Journal: PLoS ONE

    Article Title: Disturbed Relaxin Signaling Pathway and Testicular Dysfunction in Mouse Offspring upon Maternal Exposure to Simazine

    doi: 10.1371/journal.pone.0044856

    Figure Lengend Snippet: Reduced expression of target genes in the relaxin pathway in the testis of F1 mice exposed to simazine. (A) The expression of crucial target genes (Nos2, Nos3, Vegf, Mmp9) in the relaxin pathway was determined by qRT-PCR on the testis of young adult F1 control or 500 µg/kg simazine-exposed mice. The data (mean ± SEM) are from six testicular analyses performed in triplicate for each group and are shown as relative fold changes. Asterisks indicate significant differences compared with the control (* p

    Article Snippet: Simazine-induced Decreased Rxfp1 Expression and NO Production in Leydig Cells in vitro The binding of relaxin to Rxfp1 increases transcription of its target genes, including Nos2, leading to stimulated production of NO , .

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR

    Model for the potentiation of TNF α -induced hepatotoxicity, oxidative stress mitochondrial dysfunction, and activation of MAPK by pyrazole induction of CYP2E1. Pyrazole induction of CYP2E1 coupled to TNF α induction of iNOS results in elevated oxidative/nitrosative stress in hepatocytes. This results in activation of JNK and p38 MAPK which, along with the elevated ROS/RNS, damage mitochondrial function ultimately leading to liver injury.

    Journal: International Journal of Hepatology

    Article Title: CYP2E1 Sensitizes the Liver to LPS- and TNF α-Induced Toxicity via Elevated Oxidative and Nitrosative Stress and Activation of ASK-1 and JNK Mitogen-Activated Kinases

    doi: 10.1155/2012/582790

    Figure Lengend Snippet: Model for the potentiation of TNF α -induced hepatotoxicity, oxidative stress mitochondrial dysfunction, and activation of MAPK by pyrazole induction of CYP2E1. Pyrazole induction of CYP2E1 coupled to TNF α induction of iNOS results in elevated oxidative/nitrosative stress in hepatocytes. This results in activation of JNK and p38 MAPK which, along with the elevated ROS/RNS, damage mitochondrial function ultimately leading to liver injury.

    Article Snippet: NOS2 (iNOS) knockout mice (B6-129P2) and genetic background control B6-129PF2/J mice were purchased from Jackson Laboratory and treated with saline or pyrazole alone or TNFα alone or pyrazole plus TNFα .

    Techniques: Activation Assay

    TNF α -plus-pyrazole-induced hepatotoxicity and oxidative stress are decreased in iNOS knockout mice. B6-129 WT mice and B6-129 iNOS knockout mice (NOS2−/−) were treated with either saline or pyrazole alone or TNF α alone or pyrazole plus TNF α for 3 days followed by assays of (a) ALT/AST, (b) TBARS, and (c) GSH. Note: liver injury and oxidant stress were much lower in the NOS2−/− mice than the WT mice indicating a role for NO and NO metabolites in the TNF α -plus-pyrazole-induced liver injury and oxidative stress.

    Journal: International Journal of Hepatology

    Article Title: CYP2E1 Sensitizes the Liver to LPS- and TNF α-Induced Toxicity via Elevated Oxidative and Nitrosative Stress and Activation of ASK-1 and JNK Mitogen-Activated Kinases

    doi: 10.1155/2012/582790

    Figure Lengend Snippet: TNF α -plus-pyrazole-induced hepatotoxicity and oxidative stress are decreased in iNOS knockout mice. B6-129 WT mice and B6-129 iNOS knockout mice (NOS2−/−) were treated with either saline or pyrazole alone or TNF α alone or pyrazole plus TNF α for 3 days followed by assays of (a) ALT/AST, (b) TBARS, and (c) GSH. Note: liver injury and oxidant stress were much lower in the NOS2−/− mice than the WT mice indicating a role for NO and NO metabolites in the TNF α -plus-pyrazole-induced liver injury and oxidative stress.

    Article Snippet: NOS2 (iNOS) knockout mice (B6-129P2) and genetic background control B6-129PF2/J mice were purchased from Jackson Laboratory and treated with saline or pyrazole alone or TNFα alone or pyrazole plus TNFα .

    Techniques: Knock-Out, Mouse Assay, AST Assay

    May Grünwald-Giemsa-stained lymphocyte of a control subject ( a ); and a confocal image showing inducible Nitric Oxide Synthase (iNOS) positivity ( b ); and cytochrome C positivity ( c ) lymphocytes of a placebo treated critically-ill patient. Scale bar = 20μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Melatonin Pharmacological Blood Levels Increase Total Antioxidant Capacity in Critically Ill Patients

    doi: 10.3390/ijms18040759

    Figure Lengend Snippet: May Grünwald-Giemsa-stained lymphocyte of a control subject ( a ); and a confocal image showing inducible Nitric Oxide Synthase (iNOS) positivity ( b ); and cytochrome C positivity ( c ) lymphocytes of a placebo treated critically-ill patient. Scale bar = 20μm.

    Article Snippet: The lymphocytes were incubated in 1% bovine serum albumin (BSA) for 1 h at room temperature and then overnight at 4 °C with primary antibodies against rabbit polyclonal NOS2 (iNOS) (diluted 1:50; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or mouse monoclonal cytochrome C (diluted 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

    Techniques: Staining