Journal: Journal of visualized experiments : JoVE

Article Title: Analysis of Histone Antibody Specificity with Peptide Microarrays

doi: 10.3791/55912

Figure Lengend Snippet: Materials

Article Snippet: Compatibility with the other arrayers can be configured upon request. table ft1 table-wrap mode="anchored" t5 caption a7 Name Company Catalog Number Comments Printing Buffer ArrayIt PPB BSA Omnipure 2390 Streptavidin-coated glass microscope slides Greiner Bio-one 439003-25 polypropylene 384 well plate Greiner Bio-one 784201 Biotin-fluorescein Sigma 53608 contact microarray printer Aushon 2470 Aushon 2470 Microarray Printer contact microarray printer Gene Machines OmniGrid 100 OmniGrid Microarray Printer PBS Invitrogen 14190 Blocking Buffer ArrayIt SBB Hydrophobic wax pen Vector Labs H-4000 ImmEdge Hydrophobic Barrier PAP Pen Silicon Gasket Grace Bio-labs 622511 Hybridization Vessel Thermo Scientific 267061 or similar vessel Fluorescent-dye conjugated secondary antibody Life Technologies A-21244 Alexa Fluor 647 (anti-rabbit) Fluorescent-dye conjugated secondary antibody Life Technologies A-21235 Alexa Fluor 647 (anti-mouse) Wax Imprinter ArrayIt MSI48 Tween-20 Omnipure 9490 Microarray Scanner Innopsys InnoScan 1100AL or equivalent microarray scanner EipTitan Histone Peptide Microarray Epicypher 112001 AbSurance Pro Histone Peptide Microarray Millipore 16668 MODified Histone Peptide Array Active Motif 13001 Histone Code Peptide Microarrays JPT His_MA_01 Wax Royal Oak GulfWax for wax imprinter Humidified Microarray Slide Hybridization Chamber VWR 97000-284 High throughput microscope slide washing chamber ArrayIt HTW Microscope slide centrifuge VWR 93000-204 Antibody 1 Abcam 8898 Antibody 2 Millipore 07-473 Biotinylated histone peptide EpiCypher 12-0001 Example peptide.

Techniques: Microscopy, Microarray, Blocking Assay, Plasmid Preparation, Hybridization, Peptide Microarray, Modification, High Throughput Screening Assay