Journal: Science translational medicine

Article Title: Preexisting immunity shapes distinct antibody landscapes after influenza virus infection and vaccination in humans

doi: 10.1126/scitranslmed.abd3601

Figure Lengend Snippet: (A) Pie charts show binding of 2010–2011 trivalent and 2014–2015 quadrivalent vaccination-induced mAbs to a panel of hemagglutinin (HA), neuraminidase (NA), and nucleoprotein (NP) recombinant proteins by ELISA. (B) Pie charts show binding of 2015–2016 H1N1 and 2014–2015 H3N2 infection–induced mAbs to a panel of HA, NA, and NP recombinant proteins by ELISA. Recombinant proteins were chosen from the representative influenza A vaccine strains within each vaccine (table S2) or from viruses bearing resemblance to recently circulating strains during the year of mAb isolation from infected individuals (2014 H3N2: A/Switzerland/9715293/2013, A/Hong Kong/4801/2014; 2015 H1N1: A/California/7/2009, A/Michigan/45/2015). mAbs in the “Other” category bind virus, but not HA, NA, or NP, and likely bind other undetermined influenza virus antigens. (C) Pie charts demonstrate the percent of total isolated mAbs with HAI activity isolated from both cohorts (top) and the antigen reactivity of HAI− mAbs within each cohort (bottom panel). (D) The percentage of mAbs with HAI activity was compared in individuals from infected (n = 7) and vaccinated cohorts (n = 16). Numbers in the center of each pie chart indicate the number of mAbs tested. Statistical significance was determined by chi-square test (****P < 0.0001; *P = 0.0285) (A to C) and unpaired nonparametric Mann-Whitney test (***P = 0.0001) (D). Data are representative of two to three independent experiments performed in duplicate.

Article Snippet: Recombinant NP proteins derived from A/Puerto Rico/8/1934 (H1N1) and A/Aichi/2/1968 (H3N2) were obtained from Sino Biological.

Techniques: Binding Assay, Recombinant, Enzyme-linked Immunosorbent Assay, Infection, Isolation, Activity Assay, MANN-WHITNEY

Journal: Science translational medicine

Article Title: Preexisting immunity shapes distinct antibody landscapes after influenza virus infection and vaccination in humans

doi: 10.1126/scitranslmed.abd3601

Figure Lengend Snippet: (A) Pie charts display percentages of all vaccination- and infection-induced mAbs with virus neutralization activity as assessed by microneutralization or plaque reduction assay using Madin-Darby canine kidney cell lines. (B) The overall potency of neutralizing and non-neutralizing vaccination-induced mAbs (n = 164) was compared to infection-induced mAbs (H1N1, n = 56; H3N2, n = 51), depicted as microneutralization IC50 values. Non-neutralizing mAbs are displayed on the red line above the highest test concentration at 150 μg/ml. N.S., not significant. (C) The potency of all neutralizing mAbs induced by the quadrivalent vaccine (n = 92) was compared to the potency of neutralizing infection–induced mAbs (H1N1, n = 21; H3N2, n = 10), depicted as microneutralization IC50 values. (D and E) Bar charts show the antigen reactivity of neutralizing and non-neutralizing H1N1 (D) and H3N2 (E) infection-induced mAbs. (F and G) Bar charts display the percent of total neutralizing and non-neutralizing mAbs induced by infection (F) and vaccination (G), subset by antigen reactivity. (H) Pie charts demonstrate the percentage of neutralizing and non-neutralizing stalk domain–reactive mAbs binding a broadly neutralizing stalk epitope, as determined by a CR9114 competition ELISA (top) or mAbs binding undefined stalk epitopes, determined by ELISA against a headless HA stalk construct and chimeric HA (bottom). (I and J) The potency of HA- and NA-reactive mAbs induced by infection (I) was compared to the potency of HA-reactive mAbs induced by vaccination (J). The numbers in the center of or below each chart indicate the number of mAbs tested. Statistical significance was determined by chi-square test (****P < 0.0001) (A), Fisher’s exact test (*P = 0.0427) (H), or unpaired nonparametric Kruskal-Wallis test with Dunn’s correction for multiple comparisons (B, C, I, and J). Data are representative of two independent experiments performed in duplicate.

Article Snippet: Recombinant NP proteins derived from A/Puerto Rico/8/1934 (H1N1) and A/Aichi/2/1968 (H3N2) were obtained from Sino Biological.

Techniques: Infection, Neutralization, Activity Assay, Concentration Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Construct

Journal: Science translational medicine

Article Title: Preexisting immunity shapes distinct antibody landscapes after influenza virus infection and vaccination in humans

doi: 10.1126/scitranslmed.abd3601

Figure Lengend Snippet: (A) Bar charts display the composition of H1N1 and H3N2 infection– and vaccination-induced mAb cocktails. For the infection cocktails, all H1N1 mAbs were originally induced by H1N1 infection, and all H3N2 mAbs were originally induced by H3N2 infection. The vaccination cocktails were composed of either H1N1- or H3N2-reactive vaccination-induced mAbs. Each cocktail reflects the antigen reactivity and neutralization frequencies seen in our analyses. (B and C) Survival and weight loss curves display in vivo prophylactic protective ability of H1N1 infection– and vaccination-induced mAb cocktails administered intraperitoneally at 1 mg/kg to 6- to 8-week-old female BALB/c mice challenged with 10 LD50 mouse-adapted A/Netherlands/602/2009 H1N1 virus. (D and E) Survival and weight loss curves display in vivo prophylactic protective ability of H3N2 infection and vaccination-induced mAb cocktails administered intraperitoneally at 1 mg/kg to 6- to 8-week-old female BALB/c mice challenged with 10 LD50 mouse-adapted A/Philippines/2/1982 H3N2 virus. (F to I) Survival and weight loss curves display in vivo prophylactic protective ability of HA head–, HA stalk domain–, NA-, and NP-reactive mAb cocktails (5 mAbs per cocktail) administered intraperitoneally at 5 mg/kg (F and G) or 1 mg/kg (H and I) to 6- to 8-week-old female BALB/c mice challenged with 10 LD50 mouse-adapted A/Netherlands/602/2009 H1N1 virus. Data are representative of two independent experiments and depicted as survival (B, D, F, and H) and weight loss (C, E, G, and I) curves. Statistical significance for survival curves was determined using a Mantel-Cox log-rank test [(B) ****P < 0.0001 and ***P = 0.0003; (D) ***P = 0.0002 and **P = 0.0080; (F) ****P < 0.0001 and **P = 0.0047; (H) ****P < 0.0001 and ***P = 0.0004]. Weight loss is presented as means ± SEM (n = 9 to 10 mice per group).

Article Snippet: Recombinant NP proteins derived from A/Puerto Rico/8/1934 (H1N1) and A/Aichi/2/1968 (H3N2) were obtained from Sino Biological.

Techniques: Infection, Neutralization, In Vivo

Journal: Science translational medicine

Article Title: Preexisting immunity shapes distinct antibody landscapes after influenza virus infection and vaccination in humans

doi: 10.1126/scitranslmed.abd3601

Figure Lengend Snippet: (A and B) Pie charts demonstrate the cross-reactivity of vaccination-induced mAbs (A) and infection-induced mAbs (B), which was inferred by ELISA binding to a panel of H1N1 and H3N2 viruses. Heterosubtypic cross-reactivity was defined on the basis of the ability of a mAb to bind to at least one or more strains opposite of the inducing subtype, such as an H1N1-induced mAb binding to one or more H3N2 strains. (C and D) Bar charts represent the percentage of infection-induced mAbs with equal or greater binding affinity to childhood strains (C) or any past strains (D) relative to contemporary strains circulating during the time of mAb isolation. Past strains in (D) include all available strains tested that were circulating before the year of the inducing strain for each cohort (H1N1 infection, n = 5; H3N2 infection, n = 6; H1N1-reactive vaccination, n = 5; H3N2-reactive vaccination, n = 3 strains analyzed). (E) The number of somatic mutations in the immunoglobulin heavy chain VH for 2014–2015 quadrivalent vaccine-induced mAbs (n = 106) was compared to H1N1 and H3N2 infection–induced mAbs combined (n = 117). (F) The number of somatic mutations in the immunoglobulin heavy chain VH for H1N1 infection–induced mAbs (n = 57) was compared to H3N2-infection induced mAbs (n = 60). The numbers in the center of or below each chart indicate the number of mAbs tested. Statistical significance was determined by chi-square or Fisher’s exact test (**P = 0.0017; *P = 0.0312; **P = 0.0012) (A to D) and unpaired nonparametric Mann-Whitney test (****P < 0.0001) (E and F; bars indicate median). Data are representative of two to three independent experiments performed in duplicate.

Article Snippet: Recombinant NP proteins derived from A/Puerto Rico/8/1934 (H1N1) and A/Aichi/2/1968 (H3N2) were obtained from Sino Biological.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Binding Assay, Isolation, MANN-WHITNEY

Journal: Science translational medicine

Article Title: Preexisting immunity shapes distinct antibody landscapes after influenza virus infection and vaccination in humans

doi: 10.1126/scitranslmed.abd3601

Figure Lengend Snippet: (A and B) The viral binding breadth of H1N1-reactive quadrivalent vaccine-induced mAbs is represented by heatmap analysis displaying affinity (KD) to contemporary and historical H1N1 and H3N2 whole viral strains (A) and bar graphs summarizing the number of homosubtypic H1N1 viral strains bound per H1N1-reactive mAb (n = 90), subset by antigen specificity (B; bars indicate median). (C and D) The viral binding breadth of H3N2-reactive quadrivalent vaccine-induced mAbs is represented by heatmap analysis displaying affinity (KD) to contemporary and historical H3N2 and H1N1 whole viral strains (C) and bar graphs summarizing the number of homosubtypic H3N2 viral strains bound per H3N2-reactive mAb (n = 18), subset by antigen specificity (D; bars indicate median). Heatmap data are depicted as ELISA binding affinity (KD) values for each individual mAb tested against the respective viruses, and antigen reactivity of each mAb is indicated by the color coding in the legend. For both heatmaps, the strains colored in red text represent the vaccinating strains present in the vaccines at the time of mAb isolation. Data are representative of two to three independent experiments performed in duplicate.

Article Snippet: Recombinant NP proteins derived from A/Puerto Rico/8/1934 (H1N1) and A/Aichi/2/1968 (H3N2) were obtained from Sino Biological.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Isolation

Journal: Science translational medicine

Article Title: Preexisting immunity shapes distinct antibody landscapes after influenza virus infection and vaccination in humans

doi: 10.1126/scitranslmed.abd3601

Figure Lengend Snippet: (A and B) The viral binding breadth of H1N1 infection–induced mAbs is represented by heatmap analysis displaying affinity (KD) for contemporary and historical H1N1 and H3N2 whole-virus strains (A) and bar graphs summarizing the number of homosubtypic H1N1 viral strains bound per H1N1 infection–induced mAb (n = 55), subset by antigen specificity (B; bars indicate median). (C and D) The viral binding breadth of H3N2 infection–induced mAbs is represented by heatmap analysis displaying affinity (KD) for contemporary and historical H3N2 and H1N1 whole viral strains (C) and bar graphs summarizing the number of homosubtypic H3N2 viral strains bound per H3N2 infection–induced mAb (n = 60), subset by antigen specificity (D; bars indicate median). Heatmap data are depicted as ELISA binding affinity (KD) values for each individual mAb tested against the respective viruses, and antigen reactivity of each mAb is indicated by the color coding in the legend. For both heatmaps, the strains colored in red text represent contemporary circulating strains during the time of mAb isolation. Data are representative of two to three independent experiments performed in duplicate.

Article Snippet: Recombinant NP proteins derived from A/Puerto Rico/8/1934 (H1N1) and A/Aichi/2/1968 (H3N2) were obtained from Sino Biological.

Techniques: Binding Assay, Infection, Enzyme-linked Immunosorbent Assay, Isolation

Journal: Frontiers in Microbiology

Article Title: Dual Detection of Hemagglutinin Proteins of H5N1 and H1N1 Influenza Viruses Based on FRET Combined With DNase I

doi: 10.3389/fmicb.2022.934475

Figure Lengend Snippet: Schematic diagram of dual detection of HA protein from influenza viruses H5N1 and H1N1 based on FRET combined with DNase I.

Article Snippet: Hemagglutinin proteins of influenza A H5N1 (H5N1A/Anhui/1/05) and H1N1 (H1N1A/New Caledonia/20/99), the targets for H5N1 and H1N1 AIV detection, were purchased from Sino Biology (Beijing, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Dual Detection of Hemagglutinin Proteins of H5N1 and H1N1 Influenza Viruses Based on FRET Combined With DNase I

doi: 10.3389/fmicb.2022.934475

Figure Lengend Snippet: Optimization of GO reaction conditions. (A) Fluorescence emission spectral signals of the H5N1-FAM probe. (B) H1N1-ROX probe in the presence of different concentrations of GO (0, 10, 20, 30, 40, 50, 60, 80, and 100 μg/ml). (C) Changes in trends of fluorescence signals of H5N1-FAM and H1N1-ROX probes in the presence of different concentrations of GO (0, 10, 20, 30, 40, 50, 60, 80, and 100 μg/ml). (D) Changes in trends of fluorescence signals at different time points (0, 1, 2, 3, 5, 10, 15, and 20 min) in the presence of GO (50 μg/ml).

Article Snippet: Hemagglutinin proteins of influenza A H5N1 (H5N1A/Anhui/1/05) and H1N1 (H1N1A/New Caledonia/20/99), the targets for H5N1 and H1N1 AIV detection, were purchased from Sino Biology (Beijing, China).

Techniques: Fluorescence

Journal: Frontiers in Microbiology

Article Title: Dual Detection of Hemagglutinin Proteins of H5N1 and H1N1 Influenza Viruses Based on FRET Combined With DNase I

doi: 10.3389/fmicb.2022.934475

Figure Lengend Snippet: Examination of cross-reactions by monitoring fluorescence spectra of the two probes. (A) Different concentrations of HA of H5N1 in the detection system. (B) Different concentrations of HA of H1N1 in the detection system. A range of HA protein concentrations (0, 200, 500, and 1,000 ng/ml) was examined at a fixed GO concentration of 50 μg/ml.

Article Snippet: Hemagglutinin proteins of influenza A H5N1 (H5N1A/Anhui/1/05) and H1N1 (H1N1A/New Caledonia/20/99), the targets for H5N1 and H1N1 AIV detection, were purchased from Sino Biology (Beijing, China).

Techniques: Fluorescence, Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Dual Detection of Hemagglutinin Proteins of H5N1 and H1N1 Influenza Viruses Based on FRET Combined With DNase I

doi: 10.3389/fmicb.2022.934475

Figure Lengend Snippet: Effects of different doses of DNase I on fluorescence recovery for (A) H5N1-FAM and (B) H1N1-ROX probes in the detection system. Effects of incubation times of DNase I on fluorescence recovery for (C) H5N1-FAM and (D) H1N1-ROX probes.

Article Snippet: Hemagglutinin proteins of influenza A H5N1 (H5N1A/Anhui/1/05) and H1N1 (H1N1A/New Caledonia/20/99), the targets for H5N1 and H1N1 AIV detection, were purchased from Sino Biology (Beijing, China).

Techniques: Fluorescence, Incubation

Journal: Frontiers in Microbiology

Article Title: Dual Detection of Hemagglutinin Proteins of H5N1 and H1N1 Influenza Viruses Based on FRET Combined With DNase I

doi: 10.3389/fmicb.2022.934475

Figure Lengend Snippet: Effects of DNase I on the sensitivity of detection of H1N1 HA. (A) Fluorescence spectra of a series of concentrations of H5N1 HA (0, 0.5, 1, 1.5, 2, 3, 4, 5, 7, 10, 15, and 20 ng/ml) in the presence of DNase I. (B) (F – F0)/F0 (fluorescence change) vs. concentration of H1N1 HA in the presence of DNase I. (C) Fluorescence intensities of a series of concentrations of H1N1 HA (0, 10, 20, 30, 50, 70, 100, 120, 150, and 200 ng/ml) in the absence of DNase I. (D) (F – F0)/F0 (fluorescence change) vs. concentration of H1N1 HA in the absence of DNase I. The insets depict a linear relationship between (F – F0)/F0 and the concentration of HA H1N1 in the presence of DNase I (B) and the absence of DNase I (D) .

Article Snippet: Hemagglutinin proteins of influenza A H5N1 (H5N1A/Anhui/1/05) and H1N1 (H1N1A/New Caledonia/20/99), the targets for H5N1 and H1N1 AIV detection, were purchased from Sino Biology (Beijing, China).

Techniques: Fluorescence, Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Dual Detection of Hemagglutinin Proteins of H5N1 and H1N1 Influenza Viruses Based on FRET Combined With DNase I

doi: 10.3389/fmicb.2022.934475

Figure Lengend Snippet: Specificity analysis of the aptamer sensor. HA proteins from H1N1 and H5N1 act as counterpoint controls for each other. Background control protein BSA and human IgG are at a concentration of 20 ng/ml.

Article Snippet: Hemagglutinin proteins of influenza A H5N1 (H5N1A/Anhui/1/05) and H1N1 (H1N1A/New Caledonia/20/99), the targets for H5N1 and H1N1 AIV detection, were purchased from Sino Biology (Beijing, China).

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Dual Detection of Hemagglutinin Proteins of H5N1 and H1N1 Influenza Viruses Based on FRET Combined With DNase I

doi: 10.3389/fmicb.2022.934475

Figure Lengend Snippet: Dual detection of the HA from H1N1 and H5N1 viruses in human serum samples.

Article Snippet: Hemagglutinin proteins of influenza A H5N1 (H5N1A/Anhui/1/05) and H1N1 (H1N1A/New Caledonia/20/99), the targets for H5N1 and H1N1 AIV detection, were purchased from Sino Biology (Beijing, China).

Techniques:

Journal: Molecules

Article Title: Synthesis of Four Pentacyclic Triterpene–Sialylglycopeptide Conjugates and Their Affinity Assays with Hemagglutinin

doi: 10.3390/molecules26040895

Figure Lengend Snippet: The thermodynamic dissociation constant K D (µM) values of compounds with H1N1(A/WSN/1933) and H5N1(A/Hong Kong/483/97) proteins.

Article Snippet: Biacore T200 instruments (GE Healthcare) were used to evaluate the binding affinity of compounds with H1N1 (A/WSN/1933) and H5N1 (A/Hong Kong/483/97), purchased from Sino Biological Inc. (Beijing, China) through SPR, as previously described [ ].

Techniques:

Journal: Molecules

Article Title: Synthesis of Four Pentacyclic Triterpene–Sialylglycopeptide Conjugates and Their Affinity Assays with Hemagglutinin

doi: 10.3390/molecules26040895

Figure Lengend Snippet: Surface plasmon resonance (SPR) assay characterization between conjugates and hemagglutinin proteins. ( a ) SCT-Asn-BA with H1N1(A/WSN/1933) protein; ( b ) SCT-Asn-OA with H5N1(A/Hong Kong/483/97) protein. Their K D values are labeled in the corresponding curves.

Article Snippet: Biacore T200 instruments (GE Healthcare) were used to evaluate the binding affinity of compounds with H1N1 (A/WSN/1933) and H5N1 (A/Hong Kong/483/97), purchased from Sino Biological Inc. (Beijing, China) through SPR, as previously described [ ].

Techniques: SPR Assay, Labeling