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  • 99
    Thermo Fisher co2 incubator
    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a <t>CO2</t> incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.
    Co2 Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 incubator/product/Thermo Fisher
    Average 99 stars, based on 6928 article reviews
    Price from $9.99 to $1999.99
    co2 incubator - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Millipore co2 incubator
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Co2 Incubator, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 incubator/product/Millipore
    Average 99 stars, based on 2592 article reviews
    Price from $9.99 to $1999.99
    co2 incubator - by Bioz Stars, 2020-09
    99/100 stars
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    94
    Thermo Fisher incubator
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator/product/Thermo Fisher
    Average 94 stars, based on 3491 article reviews
    Price from $9.99 to $1999.99
    incubator - by Bioz Stars, 2020-09
    94/100 stars
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    92
    Billups-Rothenberg modular incubator chamber
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Modular Incubator Chamber, supplied by Billups-Rothenberg, used in various techniques. Bioz Stars score: 92/100, based on 1065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/modular incubator chamber/product/Billups-Rothenberg
    Average 92 stars, based on 1065 article reviews
    Price from $9.99 to $1999.99
    modular incubator chamber - by Bioz Stars, 2020-09
    92/100 stars
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    94
    Sanyo incubator
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Incubator, supplied by Sanyo, used in various techniques. Bioz Stars score: 94/100, based on 972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator/product/Sanyo
    Average 94 stars, based on 972 article reviews
    Price from $9.99 to $1999.99
    incubator - by Bioz Stars, 2020-09
    94/100 stars
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    93
    Sanyo co2 incubator
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Co2 Incubator, supplied by Sanyo, used in various techniques. Bioz Stars score: 93/100, based on 810 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 incubator/product/Sanyo
    Average 93 stars, based on 810 article reviews
    Price from $9.99 to $1999.99
    co2 incubator - by Bioz Stars, 2020-09
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    93
    Tokai stage top incubator
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Stage Top Incubator, supplied by Tokai, used in various techniques. Bioz Stars score: 93/100, based on 1273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stage top incubator/product/Tokai
    Average 93 stars, based on 1273 article reviews
    Price from $9.99 to $1999.99
    stage top incubator - by Bioz Stars, 2020-09
    93/100 stars
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    92
    Agilent technologies antibody incubation
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Antibody Incubation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody incubation/product/Agilent technologies
    Average 92 stars, based on 1033 article reviews
    Price from $9.99 to $1999.99
    antibody incubation - by Bioz Stars, 2020-09
    92/100 stars
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    93
    Abcam antibody incubation
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Antibody Incubation, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody incubation/product/Abcam
    Average 93 stars, based on 756 article reviews
    Price from $9.99 to $1999.99
    antibody incubation - by Bioz Stars, 2020-09
    93/100 stars
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    94
    Santa Cruz Biotechnology antibody incubation
    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 <t>(COX2),</t> ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p
    Antibody Incubation, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 979 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody incubation/product/Santa Cruz Biotechnology
    Average 94 stars, based on 979 article reviews
    Price from $9.99 to $1999.99
    antibody incubation - by Bioz Stars, 2020-09
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    94
    Becton Dickinson co2 incubator
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Co2 Incubator, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 1032 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 incubator/product/Becton Dickinson
    Average 94 stars, based on 1032 article reviews
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    co2 incubator - by Bioz Stars, 2020-09
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    92
    Forma Therapeutics incubator
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Incubator, supplied by Forma Therapeutics, used in various techniques. Bioz Stars score: 92/100, based on 403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator/product/Forma Therapeutics
    Average 92 stars, based on 403 article reviews
    Price from $9.99 to $1999.99
    incubator - by Bioz Stars, 2020-09
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    92
    New Brunswick Scientific incubator shaker
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Incubator Shaker, supplied by New Brunswick Scientific, used in various techniques. Bioz Stars score: 92/100, based on 378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator shaker/product/New Brunswick Scientific
    Average 92 stars, based on 378 article reviews
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    incubator shaker - by Bioz Stars, 2020-09
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    99
    Millipore antibody incubation
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Antibody Incubation, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1883 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody incubation/product/Millipore
    Average 99 stars, based on 1883 article reviews
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    93
    New Brunswick Scientific incubator
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Incubator, supplied by New Brunswick Scientific, used in various techniques. Bioz Stars score: 93/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator/product/New Brunswick Scientific
    Average 93 stars, based on 357 article reviews
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    incubator - by Bioz Stars, 2020-09
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    93
    Memmert GmbH incubator
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Incubator, supplied by Memmert GmbH, used in various techniques. Bioz Stars score: 93/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator/product/Memmert GmbH
    Average 93 stars, based on 261 article reviews
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    incubator - by Bioz Stars, 2020-09
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    93
    Cell Signaling Technology Inc antibody incubation
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Antibody Incubation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 690 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody incubation/product/Cell Signaling Technology Inc
    Average 93 stars, based on 690 article reviews
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    antibody incubation - by Bioz Stars, 2020-09
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    93
    Forma Therapeutics co2 incubator
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
    Co2 Incubator, supplied by Forma Therapeutics, used in various techniques. Bioz Stars score: 93/100, based on 344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 incubator/product/Forma Therapeutics
    Average 93 stars, based on 344 article reviews
    Price from $9.99 to $1999.99
    co2 incubator - by Bioz Stars, 2020-09
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    94
    Thermo Fisher antibody incubation
    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
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    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
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    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
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    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% <t>CO2,</t> for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p
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    Image Search Results


    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.

    Journal: PLoS ONE

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance

    doi: 10.1371/journal.pone.0032920

    Figure Lengend Snippet: Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.

    Article Snippet: After overnight incubation, cells were treated with 5 ml of media each containing 1.5 µg/ml concentrations of nutlin-3a (in solution or nanoformulations) or 2 µg/ml of curcumin (in solution or nanoformulations) or dual drugs (1.5 µg/ml nutlin-3a+2 µg/ml curcumin) in solution or nanoformulations and then incubated for 2 days in CO2 incubator at 37°C.

    Techniques: Flow Cytometry, Cytometry, Incubation, Staining

    Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 (COX2), ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p

    Journal: Pharmaceuticals

    Article Title: Loss of miR-101-3p Promotes Transmigration of Metastatic Breast Cancer Cells through the Brain Endothelium by Inducing COX-2/MMP1 Signaling

    doi: 10.3390/ph13070144

    Figure Lengend Snippet: Ectopic downregulation of miR-101-3p in breast cancer cells promotes trans-endothelial migration through induction of COX-2-MMP1 expression. ( A ) List of has-miR-101-3p transendothelial migration-related predicted target genes in silico. ( B ) Protein–protein interaction network analysis retrieved from STRING ( http://www.string-db.org/ , version 9.1) showing the link between the different molecules involved in transmigration of breast cancer cells across the blood–brain barrier (BBB) (HBEGF, PTGS2 and ST6GALNAC5). Different colors of the lines represent the types of evidence for association: green line, neighborhood evidence; red line, fusion evidence; purple line, experimental evidence; light blue line, database evidence; black line, coexpression evidence; blue line, co-occurrence evidence; and yellow line, text-mining evidence. ( C ) Relative luciferase activity expressed as Firefly/Renilla luciferase activity. ( D ) Relative PTGS2 (COX2), ST6GALNAC5 and HBEGF mRNA and protein expression measured by real time PCR and western blot in control and anti-miR-101-3p transfected cells. ( E ) Western Blot analysis of MMP1 in the different BC cell lines and in control and anti-miR-101-3p transfected MDA-MB-231-TGL cells. MMP-1 levels released in the culture media were quantified by ELISA. ( F ) The transmigration ability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was examined by trans-endothelial migration assay. ( G ) Western Blot analysis of COX-2 and MMP1 in MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib. ( H ) Cell viability of MDA-MB-231-TGL cells treated with miR-101-3p inhibitor and/or celecoxib was measured by MTT assay. Data are mean ±SD from three independent experiments. ** p

    Article Snippet: All cells were maintained in a 95% humidified air and 5% CO2 incubator at 37 °C.

    Techniques: Migration, Expressing, In Silico, Transmigration Assay, Luciferase, Activity Assay, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Multiple Displacement Amplification, Enzyme-linked Immunosorbent Assay, MTT Assay

    α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% CO2, for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p

    Journal: Molecular Cancer

    Article Title: α-santalol inhibits the angiogenesis and growth of human prostate tumor growth by targeting vascular endothelial growth factor receptor 2-mediated AKT/mTOR/P70S6K signaling pathway

    doi: 10.1186/1476-4598-12-147

    Figure Lengend Snippet: α-santalol inhibits angiogenesis ex vivo and in vivo . (A) Aortic rings were embedded in Matrigel, treated with various concentrations of α-santalol and sunitinib in the presence or absence of VEGF and incubated in supplemented media at 37°C, 5% CO2, for 6 days. The images are representative of three experiments (× 100). (B) Bar graphs showing number of microvessel sprouts treated with α-santalol and sunitinib in the presence or absence of VEGF. Values are mean ± SEM (n = 6) of three independent experiments. ##p

    Article Snippet: Flow cytometry fluorescence-activated cell sorting analysis About 2 × 106 HUVEC or PC3 or LNCaP cells were treated with α-santalol at 37°C, 5% CO2 incubator for 24 h. The cells were collected and analyzed in a FACS Vantage SE DiVa flow cytometer (Becton Dickinson) with propidium iodide staining.

    Techniques: Ex Vivo, In Vivo, Incubation