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  • 95
    TaKaRa in fusion ecodry hd cloning kit
    In Fusion Ecodry Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion ecodry hd cloning kit/product/TaKaRa
    Average 95 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    in fusion ecodry hd cloning kit - by Bioz Stars, 2020-07
    95/100 stars
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    94
    TaKaRa in fusion hd ecodry cloning plus systems kit
    In Fusion Hd Ecodry Cloning Plus Systems Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion hd ecodry cloning plus systems kit/product/TaKaRa
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    in fusion hd ecodry cloning plus systems kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    94
    TaKaRa in fusion ecodrytm cloning system
    Nanos1 represses <t>VegT</t> translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT <t>PBE</t> (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth <t>3′UTR</t> and <t>DsRED-DEADSouth</t> 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.
    In Fusion Ecodrytm Cloning System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion ecodrytm cloning system/product/TaKaRa
    Average 94 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    in fusion ecodrytm cloning system - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Nanos1 represses VegT translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT PBE (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth 3′UTR and DsRED-DEADSouth 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.

    Journal: Development (Cambridge, England)

    Article Title: Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells

    doi: 10.1242/dev.079608

    Figure Lengend Snippet: Nanos1 represses VegT translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT PBE (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth 3′UTR and DsRED-DEADSouth 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.

    Article Snippet: VegT 3′UTR (296 nt, 2336-2631) containing the PBE (UGUAAAUA) was subcloned downstream of the DsRED open reading frame to produce DsRED- VegT PBE-DS3′UTR (In-Fusion HD EcoDry Cloning System, Clontech).

    Techniques: RNA Binding Assay, Labeling, Mutagenesis, Binding Assay, Injection, In Vivo, Reporter Assay

    VegT RNA is stabilized in PGCs after Nanos1 knockdown. ( A ) VegT PBE mediates the degradation of the reporter message DsRED. PGCs were detected by either WISH for Xpat (top panels) or for DsRED antisense probe (bottom panels) in tailbud stage 31 embryos. Note that the DsRED reporter with a PBE was specifically degraded. Arrows indicate PGCs. ( B ) Real-time PCR analysis of VegT expression at stages 8 and 10. Expression of VegT in Nanos1 knockdown samples was normalized to that of wild-type samples. Real-time PCR was performed in duplicate, and experiments were repeated three times and showed a similar pattern. Error bars indicate s.e. P -value by unpaired Student’s t -test.

    Journal: Development (Cambridge, England)

    Article Title: Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells

    doi: 10.1242/dev.079608

    Figure Lengend Snippet: VegT RNA is stabilized in PGCs after Nanos1 knockdown. ( A ) VegT PBE mediates the degradation of the reporter message DsRED. PGCs were detected by either WISH for Xpat (top panels) or for DsRED antisense probe (bottom panels) in tailbud stage 31 embryos. Note that the DsRED reporter with a PBE was specifically degraded. Arrows indicate PGCs. ( B ) Real-time PCR analysis of VegT expression at stages 8 and 10. Expression of VegT in Nanos1 knockdown samples was normalized to that of wild-type samples. Real-time PCR was performed in duplicate, and experiments were repeated three times and showed a similar pattern. Error bars indicate s.e. P -value by unpaired Student’s t -test.

    Article Snippet: VegT 3′UTR (296 nt, 2336-2631) containing the PBE (UGUAAAUA) was subcloned downstream of the DsRED open reading frame to produce DsRED- VegT PBE-DS3′UTR (In-Fusion HD EcoDry Cloning System, Clontech).

    Techniques: Real-time Polymerase Chain Reaction, Expressing