Journal: Molecular oncology

Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

doi: 10.1016/j.molonc.2012.12.002

Figure Lengend Snippet: Overexpression of KLF4 in human malignant neuroblastoma SK‐N‐DZ and IMR‐32 cells. (A) Cells were transfected with control vector (pcDNA3.1/HisB) or KLF4 plasmid vector (pcDNA3.1/KLF4‐HisB). After transfection (24 h), ...

Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

Techniques: Over Expression, Transfection, Plasmid Preparation

Journal: Molecular oncology

Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

doi: 10.1016/j.molonc.2012.12.002

Figure Lengend Snippet: Reduction in residual cell viability of SK‐N‐DZ and IMR‐32 cells after KLF4 overexpression and APG treatment. SK‐N‐DZ (A) and IMR‐32 (B) cells were transfected with various amount of KLF4 plasmid vector ...

Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

Techniques: Over Expression, Transfection, Plasmid Preparation

Journal: Molecular oncology

Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

doi: 10.1016/j.molonc.2012.12.002

Figure Lengend Snippet: Morphological and biochemical detection of apoptosis in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM APG, 100 nM KLF4 vector + 25 μM ...

Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

Techniques: Plasmid Preparation

Journal: Molecular oncology

Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

doi: 10.1016/j.molonc.2012.12.002

Figure Lengend Snippet: Regulation of expression of Bax and Bcl‐2 proteins in SK‐N‐DZ and IMR‐32 cells by KLF4. Treatments (24 h): control (CTL) cells, 100 nM Bcl‐2 shRNA, 100 nM KLF4 vector, and 100 nM ...

Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

Techniques: Expressing, shRNA, Plasmid Preparation

Journal: Molecular oncology

Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

doi: 10.1016/j.molonc.2012.12.002

Figure Lengend Snippet: Regulation of Mcl‐1, Puma, Noxa, p53, caspase‐3, and ICAD by KLF4 and APG in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM ...

Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

Techniques: Plasmid Preparation

Journal: Molecular oncology

Article Title: KLF4 overexpression and apigenin treatment down regulated anti-apoptotic Bcl-2 proteins and matrix metalloproteinases to control growth of human malignant neuroblastoma SK-N-DZ and IMR-32 cells

doi: 10.1016/j.molonc.2012.12.002

Figure Lengend Snippet: KLF4 overexpression and APG treatment reduced cell migration in SK‐N‐DZ and IMR‐32 cells. Treatments (24 h): 100 nM control (CTL) vector, 100 nM KLF4 vector, 25 μM APG, 100 nM KLF4 ...

Article Snippet: With or without Bcl‐2 knockdown by transfection with Bcl‐2 short hairpin RNA (shRNA) plasmid (Santa Cruz Biotechnology, Santa Cruz, CA), we performed KLF4 plasmid transfection in SK‐N‐DZ and IMR‐32 cells.

Techniques: Over Expression, Migration, Plasmid Preparation