Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Mean plasma pantoprazole concentration (logarithmic scale) vs. time (hr) profiles for neonatal calves ( n = 9) following intravenous (IV) single dose administration of 1.0 mg/kg of pantoprazole.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques: Clinical Proteomics, Concentration Assay

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Pantoprazole pharmacokinetic parameters following a single intravenous (1 mg/kg) administration to neonatal Holstein calves.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Tissue concentrations of pantoprazole sulfone (μg/g) in collected tissues 1, 3, and 5 days after intravenous administration of pantoprazole (1 mg/kg) from study calves.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Comparisons of pharmacokinetic parameters of pantoprazole in domestic animal species, after single dose intravenous administration.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Cell proliferation of human breast cancer cell lines . (a) MDAMB231 and (b) MDAMB468. Cells were treated with increasing concentrations of lovastatin lactone or lovastatin acid (μg/mL) for 48 hours. Data are presented as mean ± standard deviation (n = 5) * P < 0.05;** P < 0.05; *** P < 0.001.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques: Standard Deviation

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Changes in expression of proteins involved in (a) regulation of cell cycle and cell death and (b) oxidative and metabolic processes of human MDAMB231 and MDAMB468 cells . Both cell lines were treated with 8 μg/mL lovastatin lactone (Lova Lac) or lovastatin acid (Lova Ac) for 48 hours. Data represent relative spot volumes (as calculated from two-dimensional gel images of whole cell extracts; data are presented as mean ± standard deviation (n = 5) * P < 0.05;** P < 0.05; *** P < 0.001). Gel spots which showed significant differences in their volume between the control and lovastatin-treated cells were cut-out, proteins were digested and analyzed using liquid chromatography (LC) mass spectrometry (MS)/MS analysis. In MDAMB231 cells they were identified as GTPase-activating protein SH3-domain-binding protein 1 (G3BP1), TNF type 1 receptor-associated protein (TRAP1) and glutathione S-transferase (GST) omega proteins (Table 1a), whereas the spot belonging to citrate lyase beta and sterol carrier protein 2 (SCP-2) originated from MDAMB468 cells (Table 1b). Cofilin1/2 was identified as upregulated in both cell lines. The image and changes as observed in MDAMB231 cells is shown.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques: Expressing, Two-Dimensional Gel Electrophoresis, Standard Deviation, Control, Liquid Chromatography, Mass Spectrometry, Tandem Mass Spectroscopy, Binding Assay

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Western blot analysis of proteins involved in small GTPase-mediated cell signaling . Breast cancer cell lines MDAMB231 and MDAMB468 were treated with 8 μg/mL lovastatin lactone (Lova Lac) or lovastatin acid (Lova Ac) for 48 hours. For key proteins, western blot analysis was performed based on MDAMB231 cell extracts (for Ras homolog gene family member A (RhoA), cell division cycle 42 (CDC42) and GTPase-activating protein SH3-domain-binding protein 1 - phospho form (pG3BP1)), otherwise both cell lines are shown. Densitometry data were normalized based on the amount of β-actin. Data are presented as means ± standard deviations (n = 3) * P < 0.05;** P < 0.05; *** P < 0.001). Gel images were cropped to improve the clarity and conciseness of the presentation. GDI-2, Rho GDP dissociation inhibitor 2.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques: Western Blot, Binding Assay

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Western blot analysis of proteins involved in regulation of the cell cycle including the modulation of the E2F1-Rb activity . Breast cancer cell lines MDAMB231 and MDAMB468 were treated with 8 μg/mL lovastatin lactone (Lova Lac) or lovastatin acid (Lova Ac) for 48 hours. Western blot analysis of prohibitin was performed based on MDAMB231 cell extracts, otherwise both cell lines are shown. Densitometry data were normalized based on the amount of β-actin. Data are presented as means ± standard deviations (n = 3) * P < 0.05;** P < 0.05; *** P < 0.001. Gel images were cropped to improve the clarity and conciseness of the presentation. HMGB1, high-mobility group box 1; MCM7, minichromosome maintenance protein 7; MSH2, MutS homolog 2.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques: Western Blot, Activity Assay

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Western blot analysis of proteins involved in regulation of apoptosis and AKT-signaling . Breast cancer cell lines MDAMB231 and MDAMB468 were treated with 8 μg/mL lovastatin lactone (Lova Lac) or lovastatin acid (Lova Ac) for 48 hours. For key proteins, western blot analysis of phosphatase and tensin homolog (PTEN), pAkt and N-myc downstream regulated gene 1 (NDRG1) was performed based on MDAMB231 cell extracts, otherwise both cell lines are shown. Densitometry data were normalized based on the amount of β-actin. Data are presented as means ± standard deviations (n = 3) * P < 0.05;** P < 0.05; *** P < 0.001). Gel images were cropped to improve the clarity and conciseness of the presentation. PCNA, proliferating cell nuclear antigen.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques: Western Blot

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Intracellular concentrations (nmol/g cell weight) of 13 C-labeled endogenous metabolites (glycolysis and TCA cycle intermediates, glucose) and lipid metabolites (choline-containing phospholipids, cholesterol)

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques:

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Changes in intracellular 13 C-labeled -alanine, -lactate, -glucose and -glutamine signals in MDAMB468 cells treated with 8 μg/mL lovastatin acid for 48 hours . 13 C-NMR spectra with embedded, corresponding 1 H-NMR spectra are shown (including citrate at 2.52 + 2.69 ppm). Arrows indicate the direction of signal changes (increase or decrease). Ala, alanine; Gln, glutamine; Glu, glutamine; GSH, total glutathione; Lac, lactate.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques: Labeling

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Representative 1 H-NMR spectra of MDAMB468 lipid extracts . Cells were treated with 8 μg/mL lovastatin acid for 48 hours. Arrows indicate the direction of signal changes (decrease). Chol, cholesterol (C18 and C19, CH 3 ); Δ (δ), double bond; F, fatty acid side chain; F α , F β , protons in the fatty acid chain; Fmix: -(CH 2 )n-, tCho, total choline-containing phospholipids.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques:

Journal: Breast Cancer Research : BCR

Article Title: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

doi: 10.1186/bcr2485

Figure Lengend Snippet: Schematic diagram summarizing the effects of lovastatin lactone and acid on signaling pathways as found in the present study . The solid arrows mark the directional change of proteins (up- or down-regulation). Doted arrows mark hypothesized change in protein expression/activity. CDC42, cell division cycle 42; G3BP1, GTPase-activating protein SH3-domain-binding protein 1; GDI-2, Rho GDP dissociation inhibitor 2; LIMK, LIM domain kinase; MAPK, mitogen-activated protein kinase; MCM7, minichromosome maintenance protein 7; MSH2, MutS homolog 2; NDRG1, N-myc downstream regulated gene 1; p21, cyclin-dependent kinase inhibitor 1A; PCNA, proliferating cell nuclear antigen; PI3K, phosphoinositide 3-kinase; PTEN, phosphatase and tensin homolog; Rb, retinoblastoma protein; RhoA, Ras homolog gene family member A.

Article Snippet: Lovastatin (in its lactone and hydroxy acid form) was purchased from Toronto Research Chemicals (North York, Ontario, Canada).

Techniques: Protein-Protein interactions, Expressing, Activity Assay, Binding Assay

Journal: Discover Oncology

Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

doi: 10.1007/s12672-026-04403-8

Figure Lengend Snippet: IGF2BP2 promoted the stability of FTH1 mRNA. A The immunofluorescent staining showed the subcellular localization of IGF2BP2 and FTH1 in HeLa cells. B The positive correlation within IGF2BP2 and FTH1 in clinical samples ( http://gepia.cancer-pku.cn/index.html ). C RNA immunoprecipitation (RIP) assay showed the immunoprecipitated FTH1 mRNA by anti-IGF2BP2 antibody. D RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 overexpression. E RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with METTL16 silencing (sh-METTL16-1, sh-METTL16-2). F RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 silencing (si-IGF2BP2) and METTL16 overexpression. * p < 0.05, ** p < 0.01

Article Snippet: Anti-METTL16 antibody (1:2000, 19924-1-AP, Proteintech), anti-FTH1 antibody (1:1000, 83428-1-RR, Proteintech), anti-IGF2BP2 antibody (1:1000, 11601-1-AP, Proteintech) acted as the primary antibody for incubation.

Techniques: Staining, RNA Immunoprecipitation, Immunoprecipitation, Over Expression

Journal: Oncology Letters

Article Title: Effects of 14 frequently used drugs on prostate-specific antigen expression in prostate cancer LNCaP cells

doi: 10.3892/ol.2014.1936

Figure Lengend Snippet: Frequently used drugs from the prescriptions received at the pharmacy of Gifu Pharmaceutical University.

Article Snippet: Betamethasone, amlodipine, insulin, lansoprazole, loxoprofen, metformin and warfarin were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan); allopurinol, famotidine, magnesium oxide and D-pantothenic acid were obtained from Nacalai Tesque, Inc. (Kyoto, Japan); aspirin was obtained from Merck Hoei Ltd. (Osaka, Japan); candesartan was purchased from Toronto Research Chemicals Inc. (North York, ON, Canada); and rebamipide was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

Techniques:

Journal: Nature Communications

Article Title: Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues

doi: 10.1038/s41467-026-69217-2

Figure Lengend Snippet: A Chromatin-associated proteins differentially abundant across cancer lineages relative to healthy counterparts, defined as the 1% most extreme z-values per tissue. B ACBD5 and ATP5MJ relative protein abundance in chromatin samples, based on lineage, with abundance in healthy counterpart represented as a red point, while statistically significant samples are highlighted in blue (depleted) and brown (enriched). Significantly changing proteins were determined by taking the top 1% of most differentially abundant proteins between respective cancer and normal sample (two-sided). C Immunofluorescence images of ACBD5 (top) and ATP5MJ (bottom) (green) within Tubulin-stained cells (red) in prostate cancer (PC-3) and skin cancer (A-431) cell lines. Images were obtained from the HPA dataset. Scale bar, 20 μm. D Nuclear related GO-terms enriched in ProHD interactors of metabolic enzymes, with enzymes functionally associated to one significant term depicted. E Hierarchical clustering of spearman correlations based on normalized protein abundances across chromatin samples with positive relationships indicating covarying proteins across samples. F Network of statistically significant biological processes correlated with “one-carbon by folate” pathway enzymes, based on normalized chromatin-bound protein intensities. Proteins positively correlated with “one-carbon by folate” enzymes are represented as weighted edges; edge thickness corresponds to correlation strength. G Relative protein abundance per sample for GART-POLD1 and SHMT2-MCM7 protein correlation pairs across tissue types. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies used were Vinculin (E1E9V) XP ® (Cell Signaling Technology #13901; 1:1000), NDUFS3 (abcam #ab177471, 1:1000), SDHA (Thermo Fisher Scientific; #PA5-66693; 1:1000), UQCRB (Thermo Fisher Scientific; #PA5-106324; 1:1000), COX4 (Thermo Fisher Scientific; #PA5-19471; 1:1000), COX5A (Thermo Fisher Scientific; #PA5-27432; 1:1000), ATP5A1 (abcam; #ab14748; 1:1000), FDX1 (Thermo Fisher Scientific #PA5-59653, 1:1000), Histone H3 (1B1B2) (Cell Signaling Technology #14269, 1:10000), MTHFD1 (ProteinTech; #10794-1-AP; 1:1000), ATIC (ProteinTech; #11099-1-AP; 1:1000), GART (ProteinTech; #13659-1-AP; 1:1000), SHMT2 (ProteinTech; #10726-1-AP; 1:1000).

Techniques: Quantitative Proteomics, Immunofluorescence, Staining