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Image Search Results
Journal:
Article Title: Development of DNA Vaccines against Hemolytic-Uremic Syndrome in a Murine Model
doi: 10.1128/IAI.71.7.3971-3978.2003
Figure Lengend Snippet: Specific antibody binding to recombinant Stx2ΔA protein. Serum samples from adult (A and B) and newborn (C and D) mice immunized with 50 or 10 μg, respectively, of the corresponding DNA vaccine were tested by ELISA on recombinant-Stx2ΔA-coated plates. Serum samples from mice vaccinated with pStx2B and pStx2ΔA, alone or together with pGM-CSF, were collected at 15, 30, or 60 days after the initial vaccination dose and tested by serial dilutions for the ability to bind recombinant Stx2ΔA protein captured in microtiter wells. The graph shows mean ELISA titer values ± standard errors of the means (error bars) for each group of at least six animals, calculated as indicated in Materials and Methods. Symbols: private-char pc1, pCDNA; □, genetic vaccine without adjuvant; ▪, genetic vaccine plus pGM-CSF; +, differences significant with respect to pCDNA-vaccinated group (P < 0.05); # and *, differences significant between the indicated groups (P < 0.05). (A and C) pStx2ΔA vaccines; (B and D) pStx2B vaccines.
Article Snippet:
Techniques: Binding Assay, Recombinant, Enzyme-linked Immunosorbent Assay, Adjuvant, Vaccines