Journal: Pathologie (Heidelberg, Germany)

Article Title: The role of flow cytometry in the classification of myeloid disorders

doi: 10.1007/s00292-023-01272-8

Figure Lengend Snippet: Case 2. a Bone marrow aspirate smears are hemodiluted with presence of dysplastic neutrophils ( red arrow ) and occasional blasts ( black arrow ). b Flow cytometric analysis of the bone marrow aspirate shows an expanded CD34+ population in the CD45dim blast gate ( black arrow ) with aberrant myeloid immunophenotype (CD13-, HLADR-, MPO-)

Article Snippet: Mastocytosis , Aberrant immunophenotype, including expression of CD2, CD25, and/or CD30 , Included as diagnostic criterion; detection of associated myeloid neoplasm or concurrent lymphoid or plasma cell neoplasm , [ , ] , Pardanani et al. (2009), Blood 114.

Techniques:

Journal: Pathologie (Heidelberg, Germany)

Article Title: The role of flow cytometry in the classification of myeloid disorders

doi: 10.1007/s00292-023-01272-8

Figure Lengend Snippet: Case 3. a Bone marrow ( BM ) aspirate smears show an increase of atypical, blast-like cells with vacuolated cytoplasm. b Flow cytometric analysis of the peripheral blood ( PB ) shows a small population of myeloid progenitors with normal immunophenotype (1.7% of leukocytes). Analysis of the BM aspirate displays an abnormal, CD56+/CD45-cellpopulation (56% of all cells; in blue ), negative for all other myeloid/lymphoid in an expanded lymphoid/myeloid panel ( not shown ); CD45-erythroid progenitors ( in orange ) and normal myeloid progenitor cells ( in red ) represented 5 and 0.6% of all BM cells, respectively

Article Snippet: Mastocytosis , Aberrant immunophenotype, including expression of CD2, CD25, and/or CD30 , Included as diagnostic criterion; detection of associated myeloid neoplasm or concurrent lymphoid or plasma cell neoplasm , [ , ] , Pardanani et al. (2009), Blood 114.

Techniques:

Journal: Pathologie (Heidelberg, Germany)

Article Title: The role of flow cytometry in the classification of myeloid disorders

doi: 10.1007/s00292-023-01272-8

Figure Lengend Snippet: Flow cytometric immunophenotyping in the diagnosis and classification of myeloid neoplasms

Article Snippet: Mastocytosis , Aberrant immunophenotype, including expression of CD2, CD25, and/or CD30 , Included as diagnostic criterion; detection of associated myeloid neoplasm or concurrent lymphoid or plasma cell neoplasm , [ , ] , Pardanani et al. (2009), Blood 114.

Techniques: Biomarker Discovery, Cytometry, Diagnostic Assay, Immunohistochemistry, Expressing, Clinical Proteomics, Histopathology, Marker

Journal: Cytometry. Part B, Clinical cytometry

Article Title: Flow Cytometric Aberrancies in Plasma Cell Myeloma and MGUS – Correlation with Laboratory Parameters

doi: 10.1002/cyto.b.21624

Figure Lengend Snippet: Example of FACSDiva flow cytometric analysis - bone marrow specimen from a patient with PCM. A. Gating strategy. The cells of interest are identified by bright CD38 expression (left histogram) and cytoplasmic light chain restriction (right histogram). In the MIG (isotype control) tube, cells of interest are identified by bright CD38 expression only. B. Data Analysis. The left column is data from the MIG tube. The right column is data from the antibody-specific tube. The MIG for each fluorochrome is compared to the specific antibody for that fluorochrome. The percentage positive for each of the specific antibodies was identified after setting the MIG control population threshold at 2–4% positive.

Article Snippet: Analyses were carried out using FACSDiva immunophenotyping software (BD Biosciences).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Synchronous mantle cell lymphoma bone marrow involvement complicated with extensive-stage small cell lung cancer: A case report and literature review

doi: 10.3892/ol.2022.13579

Figure Lengend Snippet: Bone marrow flow cytometry immunophenotyping performed in August 2021. CD, cluster of differentiation.

Article Snippet: The FCM immunophenotyping analysis was performed in the Hangzhou Adicon Clinical laboratory (Hangzhou, China).

Techniques: Flow Cytometry