immunohistochemistry Search Results


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  • 99
    Thermo Fisher immunohistochemistry
    Immunohistochemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam immunohistochemistry
    Immunohistochemistry, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 3120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies immunohistochemistry
    Immunohistochemistry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 4687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology immunocytochemistry
    Immunocytochemistry, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc immunohistochemistry
    Immunohistochemistry, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson immunohistochemistry
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    Ventana Medical immunohistochemistry
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    Agilent technologies immunohistochemistry immunohistochemical staining
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry Immunohistochemical Staining, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Zeiss immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
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    Abcam immunohistochemistry ihc
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry Ihc, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Leica Microsystems immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 94/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Leica Biosystems immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sakura Finetek immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
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    Developmental Studies Hybridoma Bank immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical immunohistochemistry ihc
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry Ihc, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 93/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novocastra immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry, supplied by Novocastra, used in various techniques. Bioz Stars score: 94/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific immunohistochemistry
    Ghrelin expression in node negative breast cancer tissue was analyzed by <t>immunohistochemistry.</t> Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) <t>immunostaining.</t> Scale bar = 100 μm.
    Immunohistochemistry, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam immunohistochemistry staining
    OCA treatment alleviates CCl 4 -induced HSCs activation and liver fibrosis. (A) Serum ALT and AST levels. (B)–(E) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D) and <t>immunohistochemistry</t> of α- SMA (E). (F) Expression profiling of fibrosis-related genes. Results are mean ± SD ( n = 5), * P
    Immunohistochemistry Staining, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 699 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM immunohistochemistry
    OCA treatment alleviates CCl 4 -induced HSCs activation and liver fibrosis. (A) Serum ALT and AST levels. (B)–(E) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D) and <t>immunohistochemistry</t> of α- SMA (E). (F) Expression profiling of fibrosis-related genes. Results are mean ± SD ( n = 5), * P
    Immunohistochemistry, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies immunohistochemistry staining
    Expression and distribution of matriptase and HAI-1 in the human prostate. Paraffin-embedded human prostate tissue sections were stained by <t>immunohistochemistry</t> using three mAbs: the M32 for total matriptase ( A and B ), the M69 for activated matriptase ( E and F ), and the M19 for HAI-1 ( C and D ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. The photomicrographs in A , C , and E were taken from the ductal portions of the prostate and in B , D , and F from the acinial portions. Bar = 25 μm.
    Immunohistochemistry Staining, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc immunohistochemistry ihc
    Expression and distribution of matriptase and HAI-1 in the human prostate. Paraffin-embedded human prostate tissue sections were stained by <t>immunohistochemistry</t> using three mAbs: the M32 for total matriptase ( A and B ), the M69 for activated matriptase ( E and F ), and the M19 for HAI-1 ( C and D ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. The photomicrographs in A , C , and E were taken from the ductal portions of the prostate and in B , D , and F from the acinial portions. Bar = 25 μm.
    Immunohistochemistry Ihc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology immunohistochemistry immunohistochemical staining
    <t>Immunohistochemistry</t> and real-time PCR detect that progesterone replacement partially restores the nephrin expression in the diabetic kidney. A <t>immunohistochemical</t> stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the nephrin immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the nephrin immunostaining in the D + OVX + P group. B nephrin mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P
    Immunohistochemistry Immunohistochemical Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies immunohistochemistry immunohistochemistry
    Survival curves of marker proteins. The relationship of individual proteins evaluated by <t>immunohistochemistry</t> with survival with different cut-off points. A. 14-3-3β (positive v negative immunoreactivity), B. PHB (positive v negative immunoreactivity), C. IDH1 (negative/weak immunoreactivity v moderate/strong immunoreactivity), D. LDHB (negative/weak immunoreactivity v moderate/strong immunoreactivity), E. TCTP (negative/weak immunoreactivity v moderate/strong immunoreactivity), F. IDH1 (negative/weak/moderate immunoreactivity v strong immunoreactivity), G. MVP (negative/weak/moderate immunoreactivity v strong immunoreactivity), H. survival in each of 10 clusters identified by hierarchical cluster analysis (each cluster is numerically identified and corresponds to the clusters that are identified in the cluster analysis panel of Figure 7 ), I. survival in 2 clusters- cluster 1 and clusters 2–10 combined and J. two protein signature of 14-3-3β and ALDH1 showing that double negative tumours have a significantly better outcome.
    Immunohistochemistry Immunohistochemistry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covance immunohistochemistry
    Survival curves of marker proteins. The relationship of individual proteins evaluated by <t>immunohistochemistry</t> with survival with different cut-off points. A. 14-3-3β (positive v negative immunoreactivity), B. PHB (positive v negative immunoreactivity), C. IDH1 (negative/weak immunoreactivity v moderate/strong immunoreactivity), D. LDHB (negative/weak immunoreactivity v moderate/strong immunoreactivity), E. TCTP (negative/weak immunoreactivity v moderate/strong immunoreactivity), F. IDH1 (negative/weak/moderate immunoreactivity v strong immunoreactivity), G. MVP (negative/weak/moderate immunoreactivity v strong immunoreactivity), H. survival in each of 10 clusters identified by hierarchical cluster analysis (each cluster is numerically identified and corresponds to the clusters that are identified in the cluster analysis panel of Figure 7 ), I. survival in 2 clusters- cluster 1 and clusters 2–10 combined and J. two protein signature of 14-3-3β and ALDH1 showing that double negative tumours have a significantly better outcome.
    Immunohistochemistry, supplied by Covance, used in various techniques. Bioz Stars score: 92/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Ghrelin expression in node negative breast cancer tissue was analyzed by immunohistochemistry. Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) immunostaining. Scale bar = 100 μm.

    Journal: PLoS ONE

    Article Title: Ghrelin is a prognostic marker and a potential therapeutic target in breast cancer

    doi: 10.1371/journal.pone.0176059

    Figure Lengend Snippet: Ghrelin expression in node negative breast cancer tissue was analyzed by immunohistochemistry. Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) immunostaining. Scale bar = 100 μm.

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed using the Dako EnVision Plus-HRP Detection Kit (K401111-2, Dako, Glostrup, Denmark) according to the manufacturer’s instructions.

    Techniques: Expressing, Immunohistochemistry, Immunostaining

    OCA treatment alleviates CCl 4 -induced HSCs activation and liver fibrosis. (A) Serum ALT and AST levels. (B)–(E) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D) and immunohistochemistry of α- SMA (E). (F) Expression profiling of fibrosis-related genes. Results are mean ± SD ( n = 5), * P

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Combined obeticholic acid and apoptosis inhibitor treatment alleviates liver fibrosis

    doi: 10.1016/j.apsb.2018.11.004

    Figure Lengend Snippet: OCA treatment alleviates CCl 4 -induced HSCs activation and liver fibrosis. (A) Serum ALT and AST levels. (B)–(E) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D) and immunohistochemistry of α- SMA (E). (F) Expression profiling of fibrosis-related genes. Results are mean ± SD ( n = 5), * P

    Article Snippet: HSCs activation was detected by immunohistochemistry staining of α- SMA (Abcam, ab32575, 1:500).

    Techniques: Activation Assay, AST Assay, Staining, Immunohistochemistry, Expressing

    Combined OCA and IDN-6556 treatment combats liver fibrosis. CCl 4 -injured mice were enrolled to test the anti-fibrotic effect of co-administration of OCA and IDN-6556. (A) Serum ALT and AST levels. (B)–(F) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D), immunohistochemistry of α- SMA (E) and TUNEL staining (F). (G) Expression profiling of fibrosis-related genes. (H) Caspase activities of liver homogenate. Serum total BAs level (I) and profiles (J). Results are mean ± SD ( n = 5), * P

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Combined obeticholic acid and apoptosis inhibitor treatment alleviates liver fibrosis

    doi: 10.1016/j.apsb.2018.11.004

    Figure Lengend Snippet: Combined OCA and IDN-6556 treatment combats liver fibrosis. CCl 4 -injured mice were enrolled to test the anti-fibrotic effect of co-administration of OCA and IDN-6556. (A) Serum ALT and AST levels. (B)–(F) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D), immunohistochemistry of α- SMA (E) and TUNEL staining (F). (G) Expression profiling of fibrosis-related genes. (H) Caspase activities of liver homogenate. Serum total BAs level (I) and profiles (J). Results are mean ± SD ( n = 5), * P

    Article Snippet: HSCs activation was detected by immunohistochemistry staining of α- SMA (Abcam, ab32575, 1:500).

    Techniques: Mouse Assay, AST Assay, Staining, Immunohistochemistry, TUNEL Assay, Expressing

    Expression and distribution of matriptase and HAI-1 in the human prostate. Paraffin-embedded human prostate tissue sections were stained by immunohistochemistry using three mAbs: the M32 for total matriptase ( A and B ), the M69 for activated matriptase ( E and F ), and the M19 for HAI-1 ( C and D ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. The photomicrographs in A , C , and E were taken from the ductal portions of the prostate and in B , D , and F from the acinial portions. Bar = 25 μm.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Polarized epithelial cells secrete matriptase as a consequence of zymogen activation and HAI-1-mediated inhibition

    doi: 10.1152/ajpcell.00201.2009

    Figure Lengend Snippet: Expression and distribution of matriptase and HAI-1 in the human prostate. Paraffin-embedded human prostate tissue sections were stained by immunohistochemistry using three mAbs: the M32 for total matriptase ( A and B ), the M69 for activated matriptase ( E and F ), and the M19 for HAI-1 ( C and D ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. The photomicrographs in A , C , and E were taken from the ductal portions of the prostate and in B , D , and F from the acinial portions. Bar = 25 μm.

    Article Snippet: Immunohistochemistry staining was performed using the manufacturer's standard protocol with minor modification (Dako).

    Techniques: Expressing, Staining, Immunohistochemistry

    Distribution of matriptase and HAI-1 in human kidney. Paraffin-embedded human kidney specimens were stained by immunohistochemistry using mAbs against total matriptase ( A ), activated matriptase ( C ), or HAI-1 ( B ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. Bar = 20 μm.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Polarized epithelial cells secrete matriptase as a consequence of zymogen activation and HAI-1-mediated inhibition

    doi: 10.1152/ajpcell.00201.2009

    Figure Lengend Snippet: Distribution of matriptase and HAI-1 in human kidney. Paraffin-embedded human kidney specimens were stained by immunohistochemistry using mAbs against total matriptase ( A ), activated matriptase ( C ), or HAI-1 ( B ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. Bar = 20 μm.

    Article Snippet: Immunohistochemistry staining was performed using the manufacturer's standard protocol with minor modification (Dako).

    Techniques: Staining, Immunohistochemistry

    Immunohistochemistry and real-time PCR detect that progesterone replacement partially restores the nephrin expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the nephrin immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the nephrin immunostaining in the D + OVX + P group. B nephrin mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

    doi: 10.1186/s13098-015-0097-1

    Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement partially restores the nephrin expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the nephrin immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the nephrin immunostaining in the D + OVX + P group. B nephrin mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

    Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

    Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the VEGF-A expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the VEGF-A immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the VEGF-A immunostaining in D + OVX + P group. B VEGF-A mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

    doi: 10.1186/s13098-015-0097-1

    Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the VEGF-A expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the VEGF-A immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the VEGF-A immunostaining in D + OVX + P group. B VEGF-A mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

    Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

    Immunohistochemistry and real-time PCR detect that progesterone replacement restores the podocin expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the podocin immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the podocin immunostaining in the D + OVX + P group. B Podocin mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

    doi: 10.1186/s13098-015-0097-1

    Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement restores the podocin expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the podocin immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the podocin immunostaining in the D + OVX + P group. B Podocin mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

    Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

    Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the AT1R expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the AT1R immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the AT1R immunostaining in the D + OVX + P group. B AT1R mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

    doi: 10.1186/s13098-015-0097-1

    Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the AT1R expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the AT1R immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the AT1R immunostaining in the D + OVX + P group. B AT1R mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

    Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

    Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the TGF-β expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the TGF-β immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the TGF-β immunostaining in the D + OVX + P group. B TGF-β mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

    doi: 10.1186/s13098-015-0097-1

    Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the TGF-β expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the TGF-β immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the TGF-β immunostaining in the D + OVX + P group. B TGF-β mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

    Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

    Immunohistochemistry and real-time PCR detect that progesterone replacement increase the MMP-2 protein, but not the mRNA levels in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the MMP-2 immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the MMP-2 immunostaining in the D + OVX + P group. B MMP-2 mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

    doi: 10.1186/s13098-015-0097-1

    Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement increase the MMP-2 protein, but not the mRNA levels in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the MMP-2 immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the MMP-2 immunostaining in the D + OVX + P group. B MMP-2 mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

    Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Staining, Immunostaining

    Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the fibronectin expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the fibronectin immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the fibronectin immunostaining in the D + OVX + P group. B fibronectin mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Journal: Diabetology & Metabolic Syndrome

    Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

    doi: 10.1186/s13098-015-0097-1

    Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the fibronectin expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the fibronectin immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the fibronectin immunostaining in the D + OVX + P group. B fibronectin mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

    Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

    Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

    Survival curves of marker proteins. The relationship of individual proteins evaluated by immunohistochemistry with survival with different cut-off points. A. 14-3-3β (positive v negative immunoreactivity), B. PHB (positive v negative immunoreactivity), C. IDH1 (negative/weak immunoreactivity v moderate/strong immunoreactivity), D. LDHB (negative/weak immunoreactivity v moderate/strong immunoreactivity), E. TCTP (negative/weak immunoreactivity v moderate/strong immunoreactivity), F. IDH1 (negative/weak/moderate immunoreactivity v strong immunoreactivity), G. MVP (negative/weak/moderate immunoreactivity v strong immunoreactivity), H. survival in each of 10 clusters identified by hierarchical cluster analysis (each cluster is numerically identified and corresponds to the clusters that are identified in the cluster analysis panel of Figure 7 ), I. survival in 2 clusters- cluster 1 and clusters 2–10 combined and J. two protein signature of 14-3-3β and ALDH1 showing that double negative tumours have a significantly better outcome.

    Journal: PLoS ONE

    Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

    doi: 10.1371/journal.pone.0027718

    Figure Lengend Snippet: Survival curves of marker proteins. The relationship of individual proteins evaluated by immunohistochemistry with survival with different cut-off points. A. 14-3-3β (positive v negative immunoreactivity), B. PHB (positive v negative immunoreactivity), C. IDH1 (negative/weak immunoreactivity v moderate/strong immunoreactivity), D. LDHB (negative/weak immunoreactivity v moderate/strong immunoreactivity), E. TCTP (negative/weak immunoreactivity v moderate/strong immunoreactivity), F. IDH1 (negative/weak/moderate immunoreactivity v strong immunoreactivity), G. MVP (negative/weak/moderate immunoreactivity v strong immunoreactivity), H. survival in each of 10 clusters identified by hierarchical cluster analysis (each cluster is numerically identified and corresponds to the clusters that are identified in the cluster analysis panel of Figure 7 ), I. survival in 2 clusters- cluster 1 and clusters 2–10 combined and J. two protein signature of 14-3-3β and ALDH1 showing that double negative tumours have a significantly better outcome.

    Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

    Techniques: Marker, Immunohistochemistry

    Immunohistochemistry photomicrographs. Photomicrographs of the immunohistochemical localisation of individual proteins in normal colon, primary colorectal cancer and lymph node metastasis.

    Journal: PLoS ONE

    Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

    doi: 10.1371/journal.pone.0027718

    Figure Lengend Snippet: Immunohistochemistry photomicrographs. Photomicrographs of the immunohistochemical localisation of individual proteins in normal colon, primary colorectal cancer and lymph node metastasis.

    Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

    Techniques: Immunohistochemistry

    Tumour and normal 2D gels. Representative reference 2D gels of normal colon (A) and colon tumour (C). These are the annotated reference gels created by the Progenesis Same Spots gel image analysis software for the analysis of individual gels. The number of each spot is assigned by the image analysis software. For easier visualisation of individual spots representative non-annotated 2D gels of normal colon and colon tumour are shown in panels B and D respectively. The proteins which were validated by immunohistochemistry have been identified in D.

    Journal: PLoS ONE

    Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

    doi: 10.1371/journal.pone.0027718

    Figure Lengend Snippet: Tumour and normal 2D gels. Representative reference 2D gels of normal colon (A) and colon tumour (C). These are the annotated reference gels created by the Progenesis Same Spots gel image analysis software for the analysis of individual gels. The number of each spot is assigned by the image analysis software. For easier visualisation of individual spots representative non-annotated 2D gels of normal colon and colon tumour are shown in panels B and D respectively. The proteins which were validated by immunohistochemistry have been identified in D.

    Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

    Techniques: Software, Immunohistochemistry

    Hierarchical cluster analysis of immunohistochemical marker proteins. Graphical representation of the immunohistochemistry marker data is shown in the middle panel. The right hand panel shows the results of the hierarchical cluster analysis presented as a dendrogram with 10 individual clusters identified. The left hand panel shows an expanded segment of the graphical representation. Proteins are represented in columns and cases in rows.

    Journal: PLoS ONE

    Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

    doi: 10.1371/journal.pone.0027718

    Figure Lengend Snippet: Hierarchical cluster analysis of immunohistochemical marker proteins. Graphical representation of the immunohistochemistry marker data is shown in the middle panel. The right hand panel shows the results of the hierarchical cluster analysis presented as a dendrogram with 10 individual clusters identified. The left hand panel shows an expanded segment of the graphical representation. Proteins are represented in columns and cases in rows.

    Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

    Techniques: Immunohistochemistry, Marker