Journal: PLoS ONE

Article Title: Ghrelin is a prognostic marker and a potential therapeutic target in breast cancer

doi: 10.1371/journal.pone.0176059

Figure Lengend Snippet: Ghrelin expression in node negative breast cancer tissue was analyzed by immunohistochemistry. Representative images of ghrelin with 0 (non-immunoreactive), 1 (weak), 2 (moderate) and 3 (strong) immunostaining. Scale bar = 100 μm.

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed using the Dako EnVision Plus-HRP Detection Kit (K401111-2, Dako, Glostrup, Denmark) according to the manufacturer’s instructions.

Techniques: Expressing, Immunohistochemistry, Immunostaining

Journal: Acta Pharmaceutica Sinica. B

Article Title: Combined obeticholic acid and apoptosis inhibitor treatment alleviates liver fibrosis

doi: 10.1016/j.apsb.2018.11.004

Figure Lengend Snippet: OCA treatment alleviates CCl 4 -induced HSCs activation and liver fibrosis. (A) Serum ALT and AST levels. (B)–(E) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D) and immunohistochemistry of α- SMA (E). (F) Expression profiling of fibrosis-related genes. Results are mean ± SD ( n = 5), * P

Article Snippet: HSCs activation was detected by immunohistochemistry staining of α- SMA (Abcam, ab32575, 1:500).

Techniques: Activation Assay, AST Assay, Staining, Immunohistochemistry, Expressing

Journal: Acta Pharmaceutica Sinica. B

Article Title: Combined obeticholic acid and apoptosis inhibitor treatment alleviates liver fibrosis

doi: 10.1016/j.apsb.2018.11.004

Figure Lengend Snippet: Combined OCA and IDN-6556 treatment combats liver fibrosis. CCl 4 -injured mice were enrolled to test the anti-fibrotic effect of co-administration of OCA and IDN-6556. (A) Serum ALT and AST levels. (B)–(F) Histological analysis of liver sections. H E staining (B), Masson staining (C), Sirius Red staining (D), immunohistochemistry of α- SMA (E) and TUNEL staining (F). (G) Expression profiling of fibrosis-related genes. (H) Caspase activities of liver homogenate. Serum total BAs level (I) and profiles (J). Results are mean ± SD ( n = 5), * P

Article Snippet: HSCs activation was detected by immunohistochemistry staining of α- SMA (Abcam, ab32575, 1:500).

Techniques: Mouse Assay, AST Assay, Staining, Immunohistochemistry, TUNEL Assay, Expressing

Journal: American Journal of Physiology - Cell Physiology

Article Title: Polarized epithelial cells secrete matriptase as a consequence of zymogen activation and HAI-1-mediated inhibition

doi: 10.1152/ajpcell.00201.2009

Figure Lengend Snippet: Expression and distribution of matriptase and HAI-1 in the human prostate. Paraffin-embedded human prostate tissue sections were stained by immunohistochemistry using three mAbs: the M32 for total matriptase ( A and B ), the M69 for activated matriptase ( E and F ), and the M19 for HAI-1 ( C and D ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. The photomicrographs in A , C , and E were taken from the ductal portions of the prostate and in B , D , and F from the acinial portions. Bar = 25 μm.

Article Snippet: Immunohistochemistry staining was performed using the manufacturer's standard protocol with minor modification (Dako).

Techniques: Expressing, Staining, Immunohistochemistry

Journal: American Journal of Physiology - Cell Physiology

Article Title: Polarized epithelial cells secrete matriptase as a consequence of zymogen activation and HAI-1-mediated inhibition

doi: 10.1152/ajpcell.00201.2009

Figure Lengend Snippet: Distribution of matriptase and HAI-1 in human kidney. Paraffin-embedded human kidney specimens were stained by immunohistochemistry using mAbs against total matriptase ( A ), activated matriptase ( C ), or HAI-1 ( B ). Positive staining was observed as brown precipitates (diaminobenzidine), and nuclei were counterstained with hematoxylin. Bar = 20 μm.

Article Snippet: Immunohistochemistry staining was performed using the manufacturer's standard protocol with minor modification (Dako).

Techniques: Staining, Immunohistochemistry

Journal: Diabetology & Metabolic Syndrome

Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

doi: 10.1186/s13098-015-0097-1

Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement partially restores the nephrin expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the nephrin immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the nephrin immunostaining in the D + OVX + P group. B nephrin mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

Journal: Diabetology & Metabolic Syndrome

Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

doi: 10.1186/s13098-015-0097-1

Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the VEGF-A expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the VEGF-A immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the VEGF-A immunostaining in D + OVX + P group. B VEGF-A mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

Journal: Diabetology & Metabolic Syndrome

Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

doi: 10.1186/s13098-015-0097-1

Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement restores the podocin expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the podocin immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the podocin immunostaining in the D + OVX + P group. B Podocin mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

Journal: Diabetology & Metabolic Syndrome

Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

doi: 10.1186/s13098-015-0097-1

Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the AT1R expression in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the AT1R immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the AT1R immunostaining in the D + OVX + P group. B AT1R mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

Journal: Diabetology & Metabolic Syndrome

Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

doi: 10.1186/s13098-015-0097-1

Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the TGF-β expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the TGF-β immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the TGF-β immunostaining in the D + OVX + P group. B TGF-β mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

Journal: Diabetology & Metabolic Syndrome

Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

doi: 10.1186/s13098-015-0097-1

Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement increase the MMP-2 protein, but not the mRNA levels in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the MMP-2 immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups ( arrows ). Progesterone replacement inhibited the decrease in the MMP-2 immunostaining in the D + OVX + P group. B MMP-2 mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Staining, Immunostaining

Journal: Diabetology & Metabolic Syndrome

Article Title: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats

doi: 10.1186/s13098-015-0097-1

Figure Lengend Snippet: Immunohistochemistry and real-time PCR detect that progesterone replacement inhibits the fibronectin expression in the diabetic kidney. A immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the fibronectin immunostaining (brown staining) in the glomeruli was much stronger in the D and the D-OVX groups compared with the ND group ( arrows ). Progesterone replacement inhibited the increase in the fibronectin immunostaining in the D + OVX + P group. B fibronectin mRNA expression by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another ( P

Article Snippet: Immunohistochemistry Immunohistochemical staining was performed with the ImmunoCruz Rabbit/Mouse ABC Staining System Kit (Santa Cruz, USA) according to the manufacture protocol.

Techniques: Immunohistochemistry, Real-time Polymerase Chain Reaction, Expressing, Staining, Immunostaining

Journal: PLoS ONE

Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

doi: 10.1371/journal.pone.0027718

Figure Lengend Snippet: Survival curves of marker proteins. The relationship of individual proteins evaluated by immunohistochemistry with survival with different cut-off points. A. 14-3-3β (positive v negative immunoreactivity), B. PHB (positive v negative immunoreactivity), C. IDH1 (negative/weak immunoreactivity v moderate/strong immunoreactivity), D. LDHB (negative/weak immunoreactivity v moderate/strong immunoreactivity), E. TCTP (negative/weak immunoreactivity v moderate/strong immunoreactivity), F. IDH1 (negative/weak/moderate immunoreactivity v strong immunoreactivity), G. MVP (negative/weak/moderate immunoreactivity v strong immunoreactivity), H. survival in each of 10 clusters identified by hierarchical cluster analysis (each cluster is numerically identified and corresponds to the clusters that are identified in the cluster analysis panel of Figure 7 ), I. survival in 2 clusters- cluster 1 and clusters 2–10 combined and J. two protein signature of 14-3-3β and ALDH1 showing that double negative tumours have a significantly better outcome.

Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

Techniques: Marker, Immunohistochemistry

Journal: PLoS ONE

Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

doi: 10.1371/journal.pone.0027718

Figure Lengend Snippet: Immunohistochemistry photomicrographs. Photomicrographs of the immunohistochemical localisation of individual proteins in normal colon, primary colorectal cancer and lymph node metastasis.

Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

Techniques: Immunohistochemistry

Journal: PLoS ONE

Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

doi: 10.1371/journal.pone.0027718

Figure Lengend Snippet: Tumour and normal 2D gels. Representative reference 2D gels of normal colon (A) and colon tumour (C). These are the annotated reference gels created by the Progenesis Same Spots gel image analysis software for the analysis of individual gels. The number of each spot is assigned by the image analysis software. For easier visualisation of individual spots representative non-annotated 2D gels of normal colon and colon tumour are shown in panels B and D respectively. The proteins which were validated by immunohistochemistry have been identified in D.

Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

Techniques: Software, Immunohistochemistry

Journal: PLoS ONE

Article Title: The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis

doi: 10.1371/journal.pone.0027718

Figure Lengend Snippet: Hierarchical cluster analysis of immunohistochemical marker proteins. Graphical representation of the immunohistochemistry marker data is shown in the middle panel. The right hand panel shows the results of the hierarchical cluster analysis presented as a dendrogram with 10 individual clusters identified. The left hand panel shows an expanded segment of the graphical representation. Proteins are represented in columns and cases in rows.

Article Snippet: Immunohistochemistry Immunohistochemistry for each antibody ( ) was performed with the biotin free Dako Envision™ system (Dako, Ely, UK) using a Dako autostainer (Dako) as previously described , , .

Techniques: Immunohistochemistry, Marker