Journal: Journal of Clinical Pathology

Article Title: Comparison of annexin II, p63 and ?-methylacyl-CoA racemase immunoreactivity in prostatic tissue: a tissue microarray study

doi: 10.1136/jcp.2006.040808

Figure Lengend Snippet: Immunohistochemistry

Article Snippet: AMACR and p63 staining was performed on an automated immunohistochemistry stainer (Ventana Medical Systems, Inc., Tucsan, Arizona, USA).

Techniques: Immunohistochemistry

Journal: Clinical Proteomics

Article Title: Proteomics-based insights into mitogen-activated protein kinase inhibitor resistance of cerebral melanoma metastases

doi: 10.1186/s12014-018-9189-x

Figure Lengend Snippet: Primary melanoma cell cultures derived from cerebral metastases. Stratification of the samples by proliferation and viability assay and calculation of the IC50 for BRAF/MEK inhibitors ( a ). Sensitive cells show E-cadherin positivity and N-cadherin negativity in immunocytochemistry ( b ) and Western blot ( c ), whereas resistant cells show E-cadherin negativity and N-cadherin positivity

Article Snippet: Staining was performed on an immunohistochemistry stainer (Agilent Dako Autostainer Link 48) using a labeled streptavidin–biotin method visualized by AEC as chromogen (Agilent Dako REAL Detection System, Peroxidase/AEC, Rabbit/Mouse, #K5003).

Techniques: Derivative Assay, Viability Assay, Immunocytochemistry, Western Blot

Journal: Clinical Proteomics

Article Title: Proteomics-based insights into mitogen-activated protein kinase inhibitor resistance of cerebral melanoma metastases

doi: 10.1186/s12014-018-9189-x

Figure Lengend Snippet: Immunohistochemistry of FFPE cerebral melanoma metastases cohort (n = 22). Evaluation of good responders (n = 7) versus poor responders (n = 15) shows significant different expression of he T cell marker CD3 (orange; p value = 0.002), CD4 (green; p value = 0.025) and CD8 (blue; p value = 0.007) visualized in the scatter plot ( a ) and by examples of the staining results ( b )

Article Snippet: Staining was performed on an immunohistochemistry stainer (Agilent Dako Autostainer Link 48) using a labeled streptavidin–biotin method visualized by AEC as chromogen (Agilent Dako REAL Detection System, Peroxidase/AEC, Rabbit/Mouse, #K5003).

Techniques: Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Expressing, Marker, Staining

Journal: Oncotarget

Article Title: Notch3 overexpression enhances progression and chemoresistance of urothelial carcinoma

doi: 10.18632/oncotarget.16156

Figure Lengend Snippet: High Notch3 levels are associated with poor prognosis ( A ) Notch3 levels were assessed in 59 paraffin-embedded formalin-fixed urothelial cancer tissues and matched non-tumor tissues (from the same patient) by immunohistochemistry. Bars show the number of patients with no (IRS = 0), low (IRS = 1–4), moderate (IRS = 5–8), and high (IRS = 9–12) expression in urothelial cancer tissues. ( B ) Kaplan-Meier curve for overall survival was compared according to Notch3 levels in human urothelial cancer tissues. Samples were divided as low (IRS

Article Snippet: Protein expression was detected by EnVision immunohistochemistry (Dako, Glostrup, Denmark).

Techniques: Immunohistochemistry, Expressing

Journal: Oncotarget

Article Title: Notch3 overexpression enhances progression and chemoresistance of urothelial carcinoma

doi: 10.18632/oncotarget.16156

Figure Lengend Snippet: Notch3 knockdown decreases tumor growth in vivo ( A – C ) Notch3 promotes tumor growth in vivo . A xenograft model was established using T24 and J82 cells with or without Notch3 knockdown. Four weeks after injection of T24 and J82 cells, mice were sacrificed and tumor volumes and weights were obtained. Data are presented as mean ± SEM ( n = 6 in each group). ( D ) Ki-67 levels in T24 xenograft tissues after treatment with Notch3-shRNA or scrambled shRNA, determined by immunohistochemistry. Representative photomicrographs are presented. Scale bar, 50 μm.

Article Snippet: Protein expression was detected by EnVision immunohistochemistry (Dako, Glostrup, Denmark).

Techniques: In Vivo, Injection, Mouse Assay, shRNA, Immunohistochemistry

Journal: Oncotarget

Article Title: Notch3 overexpression enhances progression and chemoresistance of urothelial carcinoma

doi: 10.18632/oncotarget.16156

Figure Lengend Snippet: Notch3 is highly expressed in urothelial cancer Notch3 amounts in a tissue array containing 59 paraffin-embedded formalin-fixed urothelial cancer tissue samples and matched non-tumor counterparts (non-tumor tissue from the same patient) were examined by immunohistochemistry. ( A ) Representative photomicrographs of human urothelial cancer specimens. Notch3 was expressed in adjacent non-tumor tissue but that its expression was much higher in tumor tissues. ( B ) Immunoreactive scores (IRS) for Notch3 expression in urothelial cancer tissues (T) compared with non-tumor tissues (NT) from 59 urothelial cancer patients. ( C ) Notch3 protein levels in eight fresh urothelial cancer tumor specimens (T) and matched non-tumor tissue samples (NT) examined by western blot.

Article Snippet: Protein expression was detected by EnVision immunohistochemistry (Dako, Glostrup, Denmark).

Techniques: Immunohistochemistry, Expressing, Western Blot

Journal: Breast Cancer Research

Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression

doi: 10.1186/bcr1304

Figure Lengend Snippet: PDGF-A expression pattern studied by immunohistochemistry in invasive breast carcinomas. (a) Carcinoma without platelet-derived growth factor A (PDGF-A) expression (original magnification × 400); (b) Carcinoma with strong PDGF-A expression (original magnification × 200; inset × 400).

Article Snippet: Immunohistochemistry Automated immunohistochemistry (Lab Vision Autostainer LV-1; Lab Vision Corporation, Fremont, CA, USA) was performed with the streptavidin–biotin–peroxidase technique, using antibodies raised against human PDGFR-α (1:200 dilution; Lab Vision Corporation) and PDGF-A (clone N-30; 1:80 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Expressing, Immunohistochemistry, Derivative Assay

Journal: Breast Cancer Research

Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression

doi: 10.1186/bcr1304

Figure Lengend Snippet: PDGFR-α expression in invasive breast carcinomas by immunohistochemistry (streptavidin-biotin-peroxidase). (a) Platelet-derived growth factor receptor α (PDGFR-α) expression in pericytes and smooth muscle cells of a blood vessel: internal control (original magnification × 200); (b) Absence of PDGFR-α expression in neoplastic cells (original magnification × 200); (c) PDGFR-α diffuse cytoplasmic expression in neoplastic cells (original magnification × 200; inset × 400); (d) Neoplastic cells showing strong and diffuse cytoplasmic PDGFR-α expression (original magnification × 400).

Article Snippet: Immunohistochemistry Automated immunohistochemistry (Lab Vision Autostainer LV-1; Lab Vision Corporation, Fremont, CA, USA) was performed with the streptavidin–biotin–peroxidase technique, using antibodies raised against human PDGFR-α (1:200 dilution; Lab Vision Corporation) and PDGF-A (clone N-30; 1:80 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Expressing, Immunohistochemistry, Derivative Assay

Journal: Gut

Article Title: Investigation of the atypical FBXW7 mutation spectrum in human tumours by conditional expression of a heterozygous propellor tip missense allele in the mouse intestines

doi: 10.1136/gutjnl-2013-304719

Figure Lengend Snippet: Proliferation and Wnt activation in representative R482Q/+ polyps shown by immunohistochemistry. (A) haematoxylin–eosin stained section of polyp—scale bars 300 μm. (B) Ki67 expression in polyp—scale bars 300 μm. (C, D) β-Catenin expression—scale bars 150 μm; arrowheads highlight nuclear staining.

Article Snippet: Immunohistochemistry Immunohistochemistry for performed as described previously using the following antibodies: alkaline phosphatase (ab65834, Abcam), β-catenin (610154, BD), caspase3 (AF835, R and D systems), chromogranin A (ab15160, Abcam), Ki67 (TEC-3, DAKO) and lysozyme (EC3.2.1.17, DAKO).

Techniques: Activation Assay, Immunohistochemistry, Staining, Expressing

Journal: Oncotarget

Article Title: Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome

doi: 10.18632/oncotarget.19440

Figure Lengend Snippet: Oroxylin A suppressed the activation of NLRP3 inflammasome in DSS-induced chronic colitis ( A ) The protein levels of cleaved-caspase-1 (caspase-1 p10), cleaved-IL-1β and NLRP3 in colonic tissues were detected by western blot. ( B ) The mRNA levels of NLRP3, IL-1β and IL-6 in colonic homogenate were determined by real-time RT-PCR. ( C ) The NF-κB p65, IκBα phosphorylation in colonic tissues were detected by western blot. ( D ) The relative expressions of cytoplastic protein and nuclear protein NF-κB p65 in colonic tissues were assessed by western blot. ( E ) Immunohistochemistry of IL-1β, NLRP3 and p65 in colonic tissues of each group was measured. Brown colored is positive (scale bar, 100 μm). Data were shown as means ± SD ( n = 3). Statistical analysis was performed using one-way ANOVA coupled with a post hoc test. Significant differences were indicated as * P

Article Snippet: Immunohistochemistry kit was purchased from KeyGEN Biotech Inc. (Nanjing, China).

Techniques: Activation Assay, Western Blot, Quantitative RT-PCR, Immunohistochemistry

Journal: Oncotarget

Article Title: Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome

doi: 10.18632/oncotarget.19440

Figure Lengend Snippet: Oroxylin A down-regulated the activation of NLRP3 inflammasome in DSS-induced acute colitis ( A ) The protein levels of cleaved-caspase-1 (caspase-1 p10), cleaved-IL-1β and NLRP3 in colonic tissues were detected by western blot. ( B ) The serum IL-1β, colonic IL-1β and ( C ) colonic IL-6 were determined by ELISA. ( D ) The mRNA levels of NLRP3, IL-1β, IL-6 and TNF-α in colonic tissue were determined by real-time RT-PCR. ( E ) The NF-κB p65 and IκBα phosphorylation in colonic tissues were detected by western blot. ( F ) The relative expressions of cytoplastic protein NF-κB p65 and nuclear protein NF-κB p65 in colonic tissues were assessed by western blot. ( G ) Immunohistochemistry of NLRP3, IL-1β and p65 in colonic tissues of each group was measured. Brown colored is positive (scale bar, 100 μm). Data were shown as means ± SD ( n = 3). Statistical analysis was performed using one-way ANOVA coupled with a post hoc test. Significant differences were indicated as * P

Article Snippet: Immunohistochemistry kit was purchased from KeyGEN Biotech Inc. (Nanjing, China).

Techniques: Activation Assay, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Immunohistochemistry

Journal: International Journal of Dentistry

Article Title: Immunohistochemical Analysis of the Activation Status of the Akt/mTOR/pS6 Signaling Pathway in Oral Lichen Planus

doi: 10.1155/2013/743456

Figure Lengend Snippet: Immunohistochemical expression of phosphorylated mTOR (p-mTOR) in selected cases of (a) oral lichen planus (OLP), (b) normal mucosa (NM), (c) oral leukoplakia (OL), and (d) oral squamous cell carcinoma (OSCC) (immunohistochemistry, 100x magnifications).

Article Snippet: Immunohistochemistry Five micron-thick serial sections of formalin-fixed and paraffin-embedded tissues were immunostained in the Leica BOND-MAX fully automated immunohistochemistry system (Leica Biosystems Newcastle Ltd, Newcastle Upon Tyne, UK), by applying the NovoLink Polymer Detection System (Leica Biosystems Newcastle Ltd).

Techniques: Immunohistochemistry, Expressing

Journal: International Journal of Dentistry

Article Title: Immunohistochemical Analysis of the Activation Status of the Akt/mTOR/pS6 Signaling Pathway in Oral Lichen Planus

doi: 10.1155/2013/743456

Figure Lengend Snippet: Immunohistochemical expression of phosphorylated Akt at serine 473 (p-Akt) in selected cases of (a) oral lichen planus (OLP), (b) normal mucosa (NM), (c) oral leukoplakia (OL), and (d) oral squamous cell carcinoma (OSCC) (immunohistochemistry, 100x magnifications).

Article Snippet: Immunohistochemistry Five micron-thick serial sections of formalin-fixed and paraffin-embedded tissues were immunostained in the Leica BOND-MAX fully automated immunohistochemistry system (Leica Biosystems Newcastle Ltd, Newcastle Upon Tyne, UK), by applying the NovoLink Polymer Detection System (Leica Biosystems Newcastle Ltd).

Techniques: Immunohistochemistry, Expressing

Journal: International Journal of Dentistry

Article Title: Immunohistochemical Analysis of the Activation Status of the Akt/mTOR/pS6 Signaling Pathway in Oral Lichen Planus

doi: 10.1155/2013/743456

Figure Lengend Snippet: Immunohistochemical expression of phosphorylated ribosomal protein pS6 (phospho-pS6) in selected cases of (a) oral lichen planus (OLP), (b) normal mucosa (NM), (c) oral leukoplakia (OL), and (d) oral squamous cell carcinoma (OSCC) (immunohistochemistry, 100x magnifications).

Article Snippet: Immunohistochemistry Five micron-thick serial sections of formalin-fixed and paraffin-embedded tissues were immunostained in the Leica BOND-MAX fully automated immunohistochemistry system (Leica Biosystems Newcastle Ltd, Newcastle Upon Tyne, UK), by applying the NovoLink Polymer Detection System (Leica Biosystems Newcastle Ltd).

Techniques: Immunohistochemistry, Expressing

Journal: Liver International

Article Title: Elevated levels of circulating CDH5 and FABP1 in association with human drug‐induced liver injury

doi: 10.1111/liv.13174

Figure Lengend Snippet: Immunohistochemical staining. Immunohistochemistry analysis using anti‐ FABP 1 and anti‐ CDH 5 antibodies showed cytoplasmic expression patterns in liver and kidney. Skeletal and heart muscle showed negative or weak protein expression in myocytes for both candidates. CDH 5 expression was observed in blood vessels in all tissues included.

Article Snippet: FABP1 encodes a fatty acid‐binding protein found in hepatocytes of the liver, but in small quantities, also in kidney (proximal tubules) and small intestine as confirmed by the immunohistochemistry data using Human Protein Atlas antibodies.

Techniques: Immunohistochemistry, Staining, Expressing