immunofluorescence staining Search Results


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    Thermo Fisher immunofluorescence staining
    Immunofluorescence Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dapi
    Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc e cadherin
    Mouse small intestinal epithelial cells divide and maintain morphology with passage and express epithelial cell markers when cultured under modified conditional reprogramming conditions. a Representative phase contrast images of IEC monolayer colonies 1 day post-plating and 4 days after post-plating (passage 12). Dashed circle indicates colonies. Images were taken at 10× magnification. Scale bar: 100 μm. b Representative image of colony with higher magnification (20×) c Representative flow cytometry plot of EpCAM expression in IEC monolayers. Data represents IEC monolayers from 3 individual mice and diverse passages. d Representative phase contrast images of IEC monolayers colonies at early, middle and later passages. e Representative fluorescence microscopy images of IEC monolayers stained with tight junction markers <t>E-cadherin</t> and ZO-1
    E Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dapi
    Mouse small intestinal epithelial cells divide and maintain morphology with passage and express epithelial cell markers when cultured under modified conditional reprogramming conditions. a Representative phase contrast images of IEC monolayer colonies 1 day post-plating and 4 days after post-plating (passage 12). Dashed circle indicates colonies. Images were taken at 10× magnification. Scale bar: 100 μm. b Representative image of colony with higher magnification (20×) c Representative flow cytometry plot of EpCAM expression in IEC monolayers. Data represents IEC monolayers from 3 individual mice and diverse passages. d Representative phase contrast images of IEC monolayers colonies at early, middle and later passages. e Representative fluorescence microscopy images of IEC monolayers stained with tight junction markers <t>E-cadherin</t> and ZO-1
    Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dapi - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Mouse small intestinal epithelial cells divide and maintain morphology with passage and express epithelial cell markers when cultured under modified conditional reprogramming conditions. a Representative phase contrast images of IEC monolayer colonies 1 day post-plating and 4 days after post-plating (passage 12). Dashed circle indicates colonies. Images were taken at 10× magnification. Scale bar: 100 μm. b Representative image of colony with higher magnification (20×) c Representative flow cytometry plot of EpCAM expression in IEC monolayers. Data represents IEC monolayers from 3 individual mice and diverse passages. d Representative phase contrast images of IEC monolayers colonies at early, middle and later passages. e Representative fluorescence microscopy images of IEC monolayers stained with tight junction markers E-cadherin and ZO-1

    Journal: BMC Cell Biology

    Article Title: Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses

    doi: 10.1186/s12860-018-0165-0

    Figure Lengend Snippet: Mouse small intestinal epithelial cells divide and maintain morphology with passage and express epithelial cell markers when cultured under modified conditional reprogramming conditions. a Representative phase contrast images of IEC monolayer colonies 1 day post-plating and 4 days after post-plating (passage 12). Dashed circle indicates colonies. Images were taken at 10× magnification. Scale bar: 100 μm. b Representative image of colony with higher magnification (20×) c Representative flow cytometry plot of EpCAM expression in IEC monolayers. Data represents IEC monolayers from 3 individual mice and diverse passages. d Representative phase contrast images of IEC monolayers colonies at early, middle and later passages. e Representative fluorescence microscopy images of IEC monolayers stained with tight junction markers E-cadherin and ZO-1

    Article Snippet: E-cadherin (1:100; Cat# 3195; Cell Signaling Technology, Danvers, MA) and ZO-1 (1:25; Clone# R40.76; a kind gift from Dr. Alan Fanning; UNC-Capel Hill) antibodies were diluted in permeabilization buffer (5% normal goat serum/0.5% Triton-X in PBS) and incubated on cells overnight at 4 °C.

    Techniques: Cell Culture, Modification, Flow Cytometry, Cytometry, Expressing, Mouse Assay, Fluorescence, Microscopy, Staining