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  • 99
    Thermo Fisher dynabeads protein g immunocomplexes
    Dynabeads Protein G Immunocomplexes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    GE Healthcare immunocomplexes
    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The <t>immunocomplexes</t> were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.
    Immunocomplexes, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 4018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATS Bio immunocomplex mac1
    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The <t>immunocomplexes</t> were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.
    Immunocomplex Mac1, supplied by ATS Bio, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies pap immunocomplex
    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The <t>immunocomplexes</t> were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.
    Pap Immunocomplex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher immunocomplex
    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The <t>immunocomplexes</t> were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.
    Immunocomplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM immunocomplexes
    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The <t>immunocomplexes</t> were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.
    Immunocomplexes, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co immunocomplexes
    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The <t>immunocomplexes</t> were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.
    Immunocomplexes, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PerkinElmer immunocomplexes
    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The <t>immunocomplexes</t> were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.
    Immunocomplexes, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Miltenyi Biotec immunocomplex
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplex, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    3-D Matrix immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by 3-D Matrix, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2618 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Active Motif immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Active Motif, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kodak Corp immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Kodak Corp, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai immunocomplexes
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplexes, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Syntaxin immunocomplex
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Immunocomplex, supplied by Syntaxin, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies apaap immunocomplex
    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. <t>Immunocomplexes</t> were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.
    Apaap Immunocomplex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The immunocomplexes were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.

    Journal: The Journal of Cell Biology

    Article Title: A Natural Hepatocyte Growth Factor/Scatter Factor Autocrine Loop in Myoblast Cells and the Effect of the Constitutive Met Kinase Activation on Myogenic Differentiation

    doi:

    Figure Lengend Snippet: Double ectopic expression of h- met and h-HGF/ SF is required for the constitutive activation of h- met kinase. ( A ) Northern blot analysis of RNA expression levels of h- met and h-HGF/SF in clone 33 selected from C2 h- met /h-HGF/SF double transfection. Exogenous transcripts are larger than endogenous ones. ( B ) The h- met receptor expressed by clone 33 is phosphorylated on tyrosine residues. Cells were lysed at two different culture passages and immunoprecipitated with anti–human met antibodies. Proteins were resolved by 8% reducing SDSPAGE and immunoblotted with anti–human met ( α-hmet ) or anti-phosphotyrosine ( α-PTyr ) antibodies. ( C ) Phase-contrast and anti-MHC immunofluorescence micrographs of clone 33 cells exposed for 48 h in differentiation medium containing 10% HS. ( D ) Cell lysates from C2 parental cells and three single h- met –expressing C2 (C2/ h- met ) clones were immunoprecipitated with anti–murine met antiserum (C2 cells) or with anti-human met antiserum (C2/h- met clones), respectively. The immunocomplexes were resolved by 8% reducing SDS-PAGE and immunoblotted with anti–human ( α-h-met ) or anti-phosphotyrosine mAbs ( α-PTyr ). The almost undetectable tyrosine phosphorylation exhibited by h- met in comparison with the endogenous receptor suggests that it is not efficiently activated by murine HGF/SF.

    Article Snippet: Finally, the immunocomplexes were solubilized in boiling Laemmli buffer containing 5% β-mercaptoethanol, electrophoresed on 8–12% SDS polyacrylamide gels, and transferred onto nitrocellulose filters (Hybond C; Amersham Intl.) by the semidry blot method.

    Techniques: Expressing, Activation Assay, Northern Blot, RNA Expression, Transfection, Immunoprecipitation, Immunofluorescence, Clone Assay, SDS Page

    CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. Immunocomplexes were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.

    Journal: PLoS ONE

    Article Title: The Dishevelled, EGL-10 and Pleckstrin (DEP) Domain-Containing Protein DEPDC7 Binds to CARMA2 and CARMA3 Proteins, and Regulates NF-κB Activation

    doi: 10.1371/journal.pone.0116062

    Figure Lengend Snippet: CARMA2sh and CARMA2cl isoforms interact with DEPDC7. A) Schematic representation of CARMA2 sh , CARMA2 cl and DEPDC7. B-C) HEK-293 cells were transiently cotransfected with a FLAG-tagged version of DEPDC7 along with the HA-tagged version of the indicated constructs. 24 hrs later, cell lysates were prepared and immunoprecipitated with anti-FLAG mAb. Immunocomplexes were separated by SDS-PAGE and transferred onto membranes, subsequently probed with anti-HA antibody. Right panels show controls for the specificity of immunoprecipitation.

    Article Snippet: For coimmunoprecipitation experiments, cells were lysed in lysis buffer and immunocomplexes were bound to protein A/G (Roche), resolved by SDS-PAGE and analyzed by immunoblot assay.

    Techniques: Construct, Immunoprecipitation, SDS Page