immunoaffinity columns Search Results


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  • 96
    Millipore igg depletion column
    Igg Depletion Column, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg depletion column/product/Millipore
    Average 96 stars, based on 5 article reviews
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    igg depletion column - by Bioz Stars, 2020-09
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    91
    Vicam immunoaffinity column
    Immunoaffinity Column, supplied by Vicam, used in various techniques. Bioz Stars score: 91/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column/product/Vicam
    Average 91 stars, based on 170 article reviews
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    immunoaffinity column - by Bioz Stars, 2020-09
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    91
    R-Biopharm immunoaffinity column
    Immunoaffinity Column, supplied by R-Biopharm, used in various techniques. Bioz Stars score: 91/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column/product/R-Biopharm
    Average 91 stars, based on 88 article reviews
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    immunoaffinity column - by Bioz Stars, 2020-09
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    91
    Aokin AG immunoaffinity column
    Immunoaffinity Column, supplied by Aokin AG, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    R-Biopharm ochraprep immunoaffinity columns
    Ochraprep Immunoaffinity Columns, supplied by R-Biopharm, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ochraprep immunoaffinity columns/product/R-Biopharm
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    ochraprep immunoaffinity columns - by Bioz Stars, 2020-09
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    85
    Vicam immunoaffinity column iac
    Immunoaffinity Column Iac, supplied by Vicam, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column iac/product/Vicam
    Average 85 stars, based on 18 article reviews
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    immunoaffinity column iac - by Bioz Stars, 2020-09
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    91
    Agilent technologies immunoaffinity column
    Immunoaffinity Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column/product/Agilent technologies
    Average 91 stars, based on 9 article reviews
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    immunoaffinity column - by Bioz Stars, 2020-09
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    92
    Romer Labs immunoaffinity column
    Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of <t>immunoaffinity-purified</t> water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.
    Immunoaffinity Column, supplied by Romer Labs, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoaffinity column/product/Romer Labs
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    93
    Vicam ochratest immunoaffinity column
    Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of <t>immunoaffinity-purified</t> water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.
    Ochratest Immunoaffinity Column, supplied by Vicam, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 13 article reviews
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    88
    Vicam aflatest immunoaffinity column
    Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of <t>immunoaffinity-purified</t> water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.
    Aflatest Immunoaffinity Column, supplied by Vicam, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 10 article reviews
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    aflatest immunoaffinity column - by Bioz Stars, 2020-09
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    93
    Vicam afla m1 hplc immunoaffinity columns
    Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of <t>immunoaffinity-purified</t> water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.
    Afla M1 Hplc Immunoaffinity Columns, supplied by Vicam, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 12 article reviews
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    92
    Vicam aflaochra testtm immunoaffinity column
    Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of <t>immunoaffinity-purified</t> water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.
    Aflaochra Testtm Immunoaffinity Column, supplied by Vicam, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aflaochra testtm immunoaffinity column/product/Vicam
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    92
    Vicam aflazearal testtm immunoaffinity column
    Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of <t>immunoaffinity-purified</t> water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.
    Aflazearal Testtm Immunoaffinity Column, supplied by Vicam, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 6 article reviews
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    94
    Academy Bio-Medical apoe immunoaffinity column
    HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by <t>HDL-apoE</t> particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE <t>immunoaffinity</t> column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P
    Apoe Immunoaffinity Column, supplied by Academy Bio-Medical, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Academy Bio-Medical anti apoe immunoaffinity column
    HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by <t>HDL-apoE</t> particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE <t>immunoaffinity</t> column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P
    Anti Apoe Immunoaffinity Column, supplied by Academy Bio-Medical, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti apoe immunoaffinity column/product/Academy Bio-Medical
    Average 92 stars, based on 7 article reviews
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    anti apoe immunoaffinity column - by Bioz Stars, 2020-09
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    85
    OlChemIm broad spectrum anti isoprenoid cytokinin immunoaffinity column
    HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by <t>HDL-apoE</t> particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE <t>immunoaffinity</t> column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P
    Broad Spectrum Anti Isoprenoid Cytokinin Immunoaffinity Column, supplied by OlChemIm, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 10 article reviews
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    91
    Agilent technologies human 14 immunoaffinity columns
    HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by <t>HDL-apoE</t> particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE <t>immunoaffinity</t> column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P
    Human 14 Immunoaffinity Columns, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human 14 immunoaffinity columns/product/Agilent technologies
    Average 91 stars, based on 31 article reviews
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    human 14 immunoaffinity columns - by Bioz Stars, 2020-09
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    92
    Bio-Rad sepharose 4b immunoaffinity columns
    HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by <t>HDL-apoE</t> particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE <t>immunoaffinity</t> column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P
    Sepharose 4b Immunoaffinity Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies mars 14 immunoaffinity column
    HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by <t>HDL-apoE</t> particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE <t>immunoaffinity</t> column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P
    Mars 14 Immunoaffinity Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mars 14 immunoaffinity column/product/Agilent technologies
    Average 92 stars, based on 4 article reviews
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    mars 14 immunoaffinity column - by Bioz Stars, 2020-09
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    Image Search Results


    Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of immunoaffinity-purified water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.

    Journal: Nutrients

    Article Title: Probiotic Enrichment and Reduction of Aflatoxins in a Traditional African Maize-Based Fermented Food

    doi: 10.3390/nu11020265

    Figure Lengend Snippet: Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of immunoaffinity-purified water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.

    Article Snippet: The supernatant was applied to an immunoaffinity column according to the instructions of the manufacturer (AFLASTARTM R Romer Labs Inc, Union, Missouri, USA).

    Techniques: High Performance Liquid Chromatography, Fluorescence, Purification

    HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by HDL-apoE particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE immunoaffinity column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P

    Journal: Molecular Neurodegeneration

    Article Title: Cerebrovascular amyloid Angiopathy in bioengineered vessels is reduced by high-density lipoprotein particles enriched in Apolipoprotein E

    doi: 10.1186/s13024-020-00366-8

    Figure Lengend Snippet: HDL binds to both col lagen-I and to Aβ42 to form a complex and increases Aβ transport through the bioengineered vessel wall primarily by HDL-apoE particles. a SMC were grown for 3 d on 2D coverslips before incubating with 0.5 μM FITC-Aβ42 (depicted as yellow) and 200 μg/ml Alexa-633 labeled HDL (depicted as cyan). After 24 h, cells were fixed and stained for collagen-I (magenta) before imaging by confocal microscopy. White arrows show co-localization of Aβ42, HDL and collagen-I. b Black 96 well-plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA as protein control for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/mL of HDL. After 24 h and extensive PBS washes, fluorescence representing bound Aβ42 was measured at 520 nm, excitation 490 nm. c 1 μM Aβ42 were incubated either with 200 μg/mL of HDL or BSA for 24 h at 37 °C before gel-filtration chromatography separation. Fractions were dot blotted and immunodetected for Aβ (6E10) or HDL (apoA-II). The intensity of each fraction on the dot blot was quantified, normalized between 0 and 100% and graphed on the left panel. The right panel shows a representative dot blot used for quantification. d 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h, tissues were homogenized in TBS to collect soluble Aβ, which was quantified in the tissue ( c ) or circulating media ( d ) by ELISA. f 1 μM Aβ42 was injected in the tissue chamber of bioengineered vessels and 200 μg/mL of HDL was circulated through the lumen. After 24 h vessels were lysed in RIPA and protein distribution was analysed using non-denaturing native blot probed for collagen-I, apoE, apoA-II, apoA-I and Aβ (6E10). g HDL was enriched for or depleted of apoE using an apoE immunoaffinity column. Bioengineered vessels were then treated with either fraction (200 μg/mL) in the presence of 1 μM Aβ42 as above before measuring Aβ deposition in the RIPA-soluble fraction. h RIPA lysates from engineered vessels were then analysed using non-denaturing native blot probed for collagen-I and apoE. i Black 96-well plates were coated either with 50 μg/mL rat-tail collagen-I or 10% BSA for 24 h before incubating with a solution of 1 μM of FITC-Aβ42 with or without 200 μg/ml of total HDL, apoE-depleted HDL or apoE-enriched HDL. After 24 h and extensive PBS washes, fluorescence was measured at 520 nm, excitation 490 nm. FPLC data and Western blots are representative of at least 3 individual experiments. Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by Student’s t-test or one way ANOVA * P

    Article Snippet: We prepared apoE-depleted or apoE-enriched HDL using an apoE immunoaffinity column.

    Techniques: Labeling, Staining, Imaging, Confocal Microscopy, Fluorescence, Incubation, Filtration, Chromatography, Dot Blot, Injection, Enzyme-linked Immunosorbent Assay, Fast Protein Liquid Chromatography, Western Blot