Journal:

Article Title: Determination of Aflatoxins B1, B2, G1, and G2 and Ochratoxin A in Ginseng and Ginger by Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation: Collaborative Study

doi:

Figure Lengend Snippet: Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation First Action 2008

Article Snippet: Three pairs of spiked ginger samples (total 6 test samples, 2 test samples/level) at 2, 4, and 16 μg/kg total AF (AFB1 :AFB2 :AFG1 :AFG2 = 4:1:2:1) and 1, 2, and 8 μg/kg OTA. ( 2 ) Practice samples.—Eight (2 ginseng test samples and 2 ginger test samples with known concentration of aflatoxins and OTA; and 2 ginseng test samples and 2 ginger test samples with unknown toxin concentrations). ( 3 ) Blank samples.—20 g blank ginseng and 20 g blank ginger (AF < 0.1 μg/kg, OTA < 0.1 μg/kg) for additional practice purposes, in case the participants were experiencing analytical problems with practice samples. ( 4 ) Immunoaffinity columns.—Forty AflaOchraTest immunoaffinity columns (Vicam, Watertown, MA). ( 5 ) Aflatoxin stock standard solution .—In a 4 mL silanized vial (total AF 400 ng/mL in acetonitrile: AFB1 200 ng/mL, AFB2 50 ng/mL, AFG1 100 ng/mL, AFG2 50 ng/mL). ( 6 ) OTA stock standard solution.—In a 4 mL silanized vial (OTA 200 ng/mL in methanol). ( 7 ) Dry phosphate buffer powder.—One tube.

Techniques: Quantitation Assay

Journal:

Article Title: Determination of Aflatoxins B1, B2, G1, and G2 and Ochratoxin A in Ginseng and Ginger by Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation: Collaborative Study

doi:

Figure Lengend Snippet: Multitoxin Immunoaffinity Column Cleanup and Liquid Chromatographic Quantitation First Action 2008

Article Snippet: Three pairs of spiked ginger samples (total 6 test samples, 2 test samples/level) at 2, 4, and 16 μg/kg total AF (AFB1 :AFB2 :AFG1 :AFG2 = 4:1:2:1) and 1, 2, and 8 μg/kg OTA. ( 2 ) Practice samples.—Eight (2 ginseng test samples and 2 ginger test samples with known concentration of aflatoxins and OTA; and 2 ginseng test samples and 2 ginger test samples with unknown toxin concentrations). ( 3 ) Blank samples.—20 g blank ginseng and 20 g blank ginger (AF < 0.1 μg/kg, OTA < 0.1 μg/kg) for additional practice purposes, in case the participants were experiencing analytical problems with practice samples. ( 4 ) Immunoaffinity columns.—Forty AflaOchraTest immunoaffinity columns (Vicam, Watertown, MA). ( 5 ) Aflatoxin stock standard solution .—In a 4 mL silanized vial (total AF 400 ng/mL in acetonitrile: AFB1 200 ng/mL, AFB2 50 ng/mL, AFG1 100 ng/mL, AFG2 50 ng/mL). ( 6 ) OTA stock standard solution.—In a 4 mL silanized vial (OTA 200 ng/mL in methanol). ( 7 ) Dry phosphate buffer powder.—One tube.

Techniques: Quantitation Assay

Journal: Nutrients

Article Title: Probiotic Enrichment and Reduction of Aflatoxins in a Traditional African Maize-Based Fermented Food

doi: 10.3390/nu11020265

Figure Lengend Snippet: Reduction of aflatoxins B 1 , B 2 , G 1 , and G 2 during controlled fermentation of the traditional maize-based kwete. HPLC chromatograms (fluorescence at 450 nm (arbitrary units) as a function of time (hours)) of immunoaffinity-purified water-soluble fractions of kwete after 0, 12, and 24 h of fermentation.

Article Snippet: The supernatant was applied to an immunoaffinity column according to the instructions of the manufacturer (AFLASTARTM R Romer Labs Inc, Union, Missouri, USA).

Techniques: High Performance Liquid Chromatography, Fluorescence, Purification