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  • 99
    Lonza iscove s modified dulbecco s medium imdm
    Iscove S Modified Dulbecco S Medium Imdm, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iscove s modified dulbecco s medium imdm/product/Lonza
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    99
    ATCC iscove s modified dulbecco s medium
    Iscove S Modified Dulbecco S Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher imdm
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Imdm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    imdm - by Bioz Stars, 2020-10
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    99
    Thermo Fisher iscove s modified dulbecco s medium
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Iscove S Modified Dulbecco S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13006 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iscove s modified dulbecco s medium/product/Thermo Fisher
    Average 99 stars, based on 13006 article reviews
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    92
    Millipore iscove s modified dulbecco s medium imdm
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Iscove S Modified Dulbecco S Medium Imdm, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher phenol red
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Phenol Red, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 12678 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore imdm
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Imdm, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2552 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Mediatech imdm
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Imdm, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PAA Laboratories imdm
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Imdm, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher serum free imdm
    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, <t>IMDM</t> <t>Iscove</t> modified <t>Dulbecco</t> medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin
    Serum Free Imdm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, IMDM Iscove modified Dulbecco medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin

    Journal: Stem Cell Research & Therapy

    Article Title: One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

    doi: 10.1186/s13287-018-0779-3

    Figure Lengend Snippet: Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture. a Schematic of hematopoietic differentiation protocol used in this study. b Morphological changes of the Eβ-iPSC2 cells and the corrected C46 cells during hematopoietic differentiation on day 6 of OP9 coculture (scale bar = 500 μm), and Wright’s staining on days 13, 23 and 29 of differentiation. c Quantitative RT-PCR analysis of erythroid-associated transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liquid culture) of the Eβ-iPSC2 cells and the corrected C46 cells as compared to peripheral blood CD34 + cell-derived erythroblasts at day 13 (PB). Data presented as mean ± SD of triplicate samples from a representative experiment. d Western blot analysis of alpha and beta hemoglobin expression of the Eβ-iPSC2 cells and the corrected C46 cells at day 30 of differentiation as compared to peripheral blood CD34 + cell-derived erythroblasts at day 24 of erythroid liquid culture. MEM minimal essential medium, IMDM Iscove modified Dulbecco medium, FBS fetal bovine serum, SCF stem cell factor, IL interleukin, EPO erythropoietin, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, Hb hemoglobin

    Article Snippet: After 24 hours of culture (day 0), the medium was replaced with differentiation medium: day 0–1 medium, RPMI (Gibco) supplemented with 5 ng/ml of human bone morphogenetic protein 4 (hBMP4) (Peprotech), 50 ng/ml of human vascular endothelial growth factor (hVEGF) (Peprotech), 25 ng/ml of hWnt3a (R & D) and 5% Knockout serum replacement (KOSR) (Gibco); day 2 medium, RPMI supplemented with 5 ng/ml of hBMP4, 50 ng/ml of hVEGF, 20 ng/ml basic fibroblast growth factor (bFGF) (Peprotech) and 5% KOSR; day 3 medium, StemPro-34 (Invitrogen), 5 ng/ml of hBMP4, 50 ng/ml of hVEGF and 20 ng/ml bFGF; day 4–5 medium, StemPro-34, 15 ng/ml of hVEGF and 5 ng/ml bFGF; day 6 medium, 74% Iscove modified Dulbecco medium (IMDM) (Gibco), 24% Ham's F12 (Gibco), 1% B27 supplement (Gibco), 0.5% N2 supplement (Gibco), 0.5% bovine serum albumin (BSA), 50 ng/ml of hVEGF, 100 ng/ml of bFGF, 100 ng/ml of human stem cell factor (SCF) (R & D) and 25 ng/ml of hFlt3 ligand (R & D); and day 7 medium, 74% IMDM, 24% Ham's F12, 1% B27 supplement, 0.5% N2 supplement, 0.5% BSA, 50 ng/ml of hVEGF, 100 ng/ml of bFGF, 100 ng/ml of hSCF, 25 ng/ml of hFlt3 ligand, 50 ng/ml of human thrombopoietin (TPO) (R & D), 10 ng/ml of IL-6 (Peprotech), 0.5 U/ml of hEPO (Eprex) and 0.2 μM of 6-formylindolo[3,2-b]carbazole (FICZ) (Abcam).

    Techniques: Staining, Quantitative RT-PCR, Derivative Assay, Western Blot, Expressing, Modification

    Hematopoietic differentiation of iPSCs using the feeder-free system. a Schematic of the feeder-free hematopoietic differentiation protocol used in this study. b Morphological changes of the corrected C46 cells during hematopoietic differentiation. c, d Numbers of CD34, CD43, CD235a and CD71-expressing cells in adherent and nonadherent systems at day 12 of differentiation. e Representative images of CFU from HDF-iPSCs. Differentiated cells at day 12 were harvested and seeded in MethoCult media. f Numbers of CFU colonies counted on day 14 of culture in MethoCult media. Data obtained from two independent experiments. Scale bars = 200 μm. IMDM Iscove modified Dulbecco medium, BMP-4 bone morphogenetic protein 4, VEGF vascular endothelial growth factor, KOSR knockout serum replacement, bFGF basic fibroblast growth factor, BSA bovine serum albumin, SCF stem cell factor, TPO thrombopoietin, IL interleukin, FICZ 6-formylindolo[3,2-b]carbazole, HDF human dermal fibroblasts, iPSC induced pluripotent stem cell, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, CFU-E colony-forming unit erythroid, EPO erythropoietin, BFU-E burst-forming unit erythroid, GM granulocyte, macrophage, GEMM granulocyte, erythrocyte, macrophage, megakaryocyte

    Journal: Stem Cell Research & Therapy

    Article Title: One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

    doi: 10.1186/s13287-018-0779-3

    Figure Lengend Snippet: Hematopoietic differentiation of iPSCs using the feeder-free system. a Schematic of the feeder-free hematopoietic differentiation protocol used in this study. b Morphological changes of the corrected C46 cells during hematopoietic differentiation. c, d Numbers of CD34, CD43, CD235a and CD71-expressing cells in adherent and nonadherent systems at day 12 of differentiation. e Representative images of CFU from HDF-iPSCs. Differentiated cells at day 12 were harvested and seeded in MethoCult media. f Numbers of CFU colonies counted on day 14 of culture in MethoCult media. Data obtained from two independent experiments. Scale bars = 200 μm. IMDM Iscove modified Dulbecco medium, BMP-4 bone morphogenetic protein 4, VEGF vascular endothelial growth factor, KOSR knockout serum replacement, bFGF basic fibroblast growth factor, BSA bovine serum albumin, SCF stem cell factor, TPO thrombopoietin, IL interleukin, FICZ 6-formylindolo[3,2-b]carbazole, HDF human dermal fibroblasts, iPSC induced pluripotent stem cell, Eβ-iPSC2 iPSC lines derived from a patient with HbE/β - thalassemia, CFU-E colony-forming unit erythroid, EPO erythropoietin, BFU-E burst-forming unit erythroid, GM granulocyte, macrophage, GEMM granulocyte, erythrocyte, macrophage, megakaryocyte

    Article Snippet: After 24 hours of culture (day 0), the medium was replaced with differentiation medium: day 0–1 medium, RPMI (Gibco) supplemented with 5 ng/ml of human bone morphogenetic protein 4 (hBMP4) (Peprotech), 50 ng/ml of human vascular endothelial growth factor (hVEGF) (Peprotech), 25 ng/ml of hWnt3a (R & D) and 5% Knockout serum replacement (KOSR) (Gibco); day 2 medium, RPMI supplemented with 5 ng/ml of hBMP4, 50 ng/ml of hVEGF, 20 ng/ml basic fibroblast growth factor (bFGF) (Peprotech) and 5% KOSR; day 3 medium, StemPro-34 (Invitrogen), 5 ng/ml of hBMP4, 50 ng/ml of hVEGF and 20 ng/ml bFGF; day 4–5 medium, StemPro-34, 15 ng/ml of hVEGF and 5 ng/ml bFGF; day 6 medium, 74% Iscove modified Dulbecco medium (IMDM) (Gibco), 24% Ham's F12 (Gibco), 1% B27 supplement (Gibco), 0.5% N2 supplement (Gibco), 0.5% bovine serum albumin (BSA), 50 ng/ml of hVEGF, 100 ng/ml of bFGF, 100 ng/ml of human stem cell factor (SCF) (R & D) and 25 ng/ml of hFlt3 ligand (R & D); and day 7 medium, 74% IMDM, 24% Ham's F12, 1% B27 supplement, 0.5% N2 supplement, 0.5% BSA, 50 ng/ml of hVEGF, 100 ng/ml of bFGF, 100 ng/ml of hSCF, 25 ng/ml of hFlt3 ligand, 50 ng/ml of human thrombopoietin (TPO) (R & D), 10 ng/ml of IL-6 (Peprotech), 0.5 U/ml of hEPO (Eprex) and 0.2 μM of 6-formylindolo[3,2-b]carbazole (FICZ) (Abcam).

    Techniques: Expressing, Modification, Knock-Out, Derivative Assay