Journal: Advanced Science

Article Title: Mgp High‐Expressing MSCs Orchestrate the Osteoimmune Microenvironment of Collagen/Nanohydroxyapatite‐Mediated Bone Regeneration

doi: 10.1002/advs.202308986

Figure Lengend Snippet: Mgp hi MSCs influenced the functions of macrophages by activating the MDK signaling pathway. A) Unique ligand–receptor pairs between OPs2 ( Mgp hi MSCs), macrophage, and osteoclasts subclusters. B) Circle plots showing the inferred MDK, and PTN signaling networks between OPs2 and macrophages. C) Spatial feature plots showed the normalized expression of Mdk , Lrp1 , Ptn , and Ncl in tissue sections. D) Relative expression of MGP , MDK , PTN in hBMMSCs. E) The protein expression level of MDK was upregulated in MGP ‐overexpressing hBMMSCs. F) The MDK inhibitor (iMDK) treatment influences the expression levels of genes associated with inflammatory response and osteoclastogenesis. G) iMDK treatment influences the expression levels of M1/M2‐related markers (iNOS/ARG‐1) in THP‐1‐derived macrophages observed via a laser‐scanning confocal microscope. H) iMDK treatment influences the expression levels of osteoclastic markers (c‐Fos and TRAP) in THP‐1‐derived macrophages. I) iMDK treatment influences the TRAP staining of THP‐1 derived macrophages after 7 days of osteoclastic induction. +BMMSCs vector : THP‐1 derived macrophages cocultured with hBMMSCs transfected with lentiviral vector; +BMMSCs MGP : THP‐1 derived macrophages cocultured with MGP ‐overexpressing hBMMSCs.

Article Snippet: To investigate the immunoregulatory of MDK, DMSO, and iMDK (500 n m , Cat#T9460, TargetMol, USA) were added into the co‐culture system.

Techniques: Expressing, Derivative Assay, Microscopy, Staining, Plasmid Preparation, Transfection

Journal: Biomedicines

Article Title: Combined Usage of MDK Inhibitor Augments Interferon-γ Anti-Tumor Activity in the SKOV3 Human Ovarian Cancer Cell Line

doi: 10.3390/biomedicines11010008

Figure Lengend Snippet: MDK inhibition potentiates the tumoricidal effect of IFN-γ in SKOV3 cells. ( A ) CCK8 analysis of the effect of IFN-γ on the cell viability of SKOV3 cells. ( B ) CCK8 analysis of the effect of iMDK on the cell viability of SKOV3 cells. ( C ) CCK8 assays to determine the effect of the combined usage of different doses of iMDK on the tumoricidal effect of IFN-γ. Data are represented as mean ± SD, n = 3.

Article Snippet: The MDK inhibitor iMDK was bought from Merck Millipore (Darmstadt, Germany).

Techniques: Inhibition

Journal: Biomedicines

Article Title: Combined Usage of MDK Inhibitor Augments Interferon-γ Anti-Tumor Activity in the SKOV3 Human Ovarian Cancer Cell Line

doi: 10.3390/biomedicines11010008

Figure Lengend Snippet: MDK inhibition attenuates the pro-metastatic adverse effect of IFN-γ. ( A ) Real-time qPCR to examine the effect of iMDK on IFN-γ-induced MDK expression at the mRNA level in SKOV3 cells. ( B ) Western blotting assays to detect the effect of iMDK on IFN-γ-induced MDK and EMT markers expression at the protein level in SKOV3 cells. ( C ) Real-time qPCR assays to validate the role of iMDK in suppressing IFN-γ-induced EMT activation in SKOV3 cells. ( D ) Transwell assays to determine the effect of iMDK on IFN-γ-induced migration and invasion in SKOV3 cells. Data are represented as mean ± SD. ** p < 0.01, **** p < 0.0001.

Article Snippet: The MDK inhibitor iMDK was bought from Merck Millipore (Darmstadt, Germany).

Techniques: Inhibition, Expressing, Western Blot, Activation Assay, Migration