Journal: Frontiers in Cellular and Infection Microbiology

Article Title: LILRA5 + macrophages drive early oxidative stress surge in sepsis: a single-cell transcriptomic landscape with therapeutic implications

doi: 10.3389/fcimb.2025.1606401

Figure Lengend Snippet: Validation of hub features in 10 cell types. (A) Box plots revealed the expression of four feature genes (S100A9, PLBD1, MGST1, and LILRA5) in the training dataset. (B) ROC curves of our feature genes (S100A9, PLBD1, MGST1, and LILRA5) in the training dataset. (C) Box plots revealed the expression of four feature genes (S100A9, PLBD1, MGST1, and LILRA5) in the testing dataset. (D) ROC curves of four feature genes (S100A9, PLBD1, MGST1, and LILRA5) in the testing dataset. (E) The violin plot showed the expression of S100A9, PLBD1, MGST1, and LILRA5 in low-, medium-, and high-OS groups. (F) Expression of S100A9, PLBD1, MGST1, and LILRA5 in 10 cell types by the bubble plot. (G, H) The results of UMAP indicated the expression of four feature genes in 10 cell types.

Article Snippet: The membrane was blocked, incubated overnight with a primary antibody against LILRA5 and β-actin (Proteintech, USA), and then incubated with a secondary antibody for 1 h. Results were detected using a Western Blot Imaging System (4000R, CareTream, USA).

Techniques: Biomarker Discovery, Expressing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: LILRA5 + macrophages drive early oxidative stress surge in sepsis: a single-cell transcriptomic landscape with therapeutic implications

doi: 10.3389/fcimb.2025.1606401

Figure Lengend Snippet: Cell–cell communication and transcription factor analysis. (A) Cell chat analysis of all cell types. Both interaction numbers and interaction strengths are shown. (B) Scatter plot indicates the differences of incoming and outgoing interaction strengths among all cell types. (C) Top cell cytokines were shown in the heatmap across all cell types in sepsis. (D) As the quasi-temporal process unfolds, the proportions of LILRA5 + macrophage and LILRA5 − macrophage differentiated synchronously. (E) The relative expression of LILRA5 in the pseudotemporal analysis. (F) The slingshot analysis combined with the expression of LILRA5 by the UMAP plot. (G) The CytoTRACE analysis of LILRA5 + macrophage and LILRA5 − macrophage. (H) The predicting ordering of CytoTRACE by the boxplot. (I) The correlation analysis between LILRA5 expression and cytoTRACE scores.

Article Snippet: The membrane was blocked, incubated overnight with a primary antibody against LILRA5 and β-actin (Proteintech, USA), and then incubated with a secondary antibody for 1 h. Results were detected using a Western Blot Imaging System (4000R, CareTream, USA).

Techniques: Expressing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: LILRA5 + macrophages drive early oxidative stress surge in sepsis: a single-cell transcriptomic landscape with therapeutic implications

doi: 10.3389/fcimb.2025.1606401

Figure Lengend Snippet: Validation of LILRA5 in macrophages in sepsis. (A) mRNA expression of LILRA5 in the blood of the CLP mouse model by qRT-PCR. (B) mRNA expression of LILRA5 in the blood of the LPS-stimulated sepsis mouse model by qRT-PCR. (C) mRNA expression of LILRA5 after siRNA transfection by qRT-PCR. (D) The protein level of LILRA5 in THP-1 cells by Western blotting. (E) DCFH-DA probes were used to detect total ROS levels in THP-1 cells. After the DCFH-DA probe was pre-loaded into the THP-1 cells, the THP-1 cells were stimulated with LPS, and the fluorescence signal intensity of the cells was detected (** p < 0.01,*** p < 0.001,**** p < 0.001).

Article Snippet: The membrane was blocked, incubated overnight with a primary antibody against LILRA5 and β-actin (Proteintech, USA), and then incubated with a secondary antibody for 1 h. Results were detected using a Western Blot Imaging System (4000R, CareTream, USA).

Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Transfection, Western Blot, Fluorescence