Journal: PLoS ONE

Article Title: The Effects of Knockdown of Rho-Associated Kinase 1 and Zipper-Interacting Protein Kinase on Gene Expression and Function in Cultured Human Arterial Smooth Muscle Cells

doi: 10.1371/journal.pone.0116969

Figure Lengend Snippet: (A) CASMCs were transfected with siRNA to ZIPK or ROCK1 or with negative control siRNA. Cells were lysed 48 h later for qRT-PCR to quantify IL-6 and IL-1β mRNA levels. Values represent means ± SEM ( n = 7 for IL-6 and n = 6 for IL-1β). *significantly different from control (p < 0.001 except for IL-1β in ZIPK knockdown where p = 0.007). (B) IL-6 levels in the medium of control cells and cells transfected with ROCK1 or ZIPK siRNA were quantified by ELISA. Values represent means ± SEM ( n = 20 for CASMC, n = 29 for UASMC). *significantly different from control ( p < 0.002).

Article Snippet: IL-6 levels were quantified in culture supernatants of CASMC and UASMC 48 h after siRNA transfection using an ELISA kit (Human IL-6 DuoSet, DY206, R&D Systems) according to the manufacturer’s instructions.

Techniques: Transfection, Negative Control, Quantitative RT-PCR, Control, Knockdown, Enzyme-linked Immunosorbent Assay

Journal: Journal of Clinical Immunology

Article Title: Mice with Asthma Are More Resistant to Influenza Virus Infection and NK Cells Activated by the Induction of Asthma Have Potentially Protective Effects

doi: 10.1007/s10875-011-9619-2

Figure Lengend Snippet: Asthmatic model mice exhibit increased OVA-specific IgE in their serum and histopathological changes characterized by allergic asthma. C57BL/6 mice were intraperitoneally sensitized with OVA or PBS as a control every other day for 2 weeks, then allowed to rest for 3 weeks. The OVA-sensitized mice or control mice were then intranasally challenged with OVA or PBS on days −3, −2 and −1, respectively. Bloods, trachea tissues, and lungs were collected from the mice on day 0. a The OVA-specific IgE in the serum was measured by an ELISA assay. b The trachea tissues ( upper panels ) and lungs ( bottom panels ) were stained with HE. Six mice were used in each group and similar observations were obtained from each mouse

Article Snippet: The cytokine concentrations in the BALF supernatants were determined using a mouse Interferon Alpha ELISA Kit (PBL Biomedical Laboratories, NJ, USA) and a mouse IL-12p40 ELISA development kit, mouse IFN-γ ELISA development kit, mouse IL-6 ELISA development kit, mouse TNF-α ELISA development kit, mouse IL-4 ELISA development kit, mouse IL-13 ELISA development kit, or a mouse IL-22 ELISA development kit (R&D Systems, MN, USA) according to the manufacturer’s instructions, as described previously [ ].

Techniques: Control, Enzyme-linked Immunosorbent Assay, Staining

Journal: Journal of Clinical Immunology

Article Title: Mice with Asthma Are More Resistant to Influenza Virus Infection and NK Cells Activated by the Induction of Asthma Have Potentially Protective Effects

doi: 10.1007/s10875-011-9619-2

Figure Lengend Snippet: Differences were observed in the cytokine production between asthmatic mice and control mice during influenza virus infection. C57BL/6 mice were sensitized and challenged with OVA or PBS. Subsequently, the mice were infected with 100 pfu of the influenza virus. BALF samples from the mice were collected on day 0 before viral infection and on days 2, 4, and 6 after influenza virus infection, and the cytokine concentrations in the BALF supernatants were measured by ELISA assays. Six mice were used in each group. These results are representative of two independent experiments. * p < 0.05 compared to the cytokine production of control mice. These results are representative of two independent experiments

Article Snippet: The cytokine concentrations in the BALF supernatants were determined using a mouse Interferon Alpha ELISA Kit (PBL Biomedical Laboratories, NJ, USA) and a mouse IL-12p40 ELISA development kit, mouse IFN-γ ELISA development kit, mouse IL-6 ELISA development kit, mouse TNF-α ELISA development kit, mouse IL-4 ELISA development kit, mouse IL-13 ELISA development kit, or a mouse IL-22 ELISA development kit (R&D Systems, MN, USA) according to the manufacturer’s instructions, as described previously [ ].

Techniques: Control, Virus, Infection, Enzyme-linked Immunosorbent Assay