Journal: bioRxiv

Article Title: IL-1-conferred gene expression pattern in ERα + BCa and AR + PCa cells is intrinsic to ERα − BCa and AR − PCa cells and promotes cell survival

doi: 10.1101/773978

Figure Lengend Snippet: RT-qPCR was performed for select genes for MCF7 and LNCaP cell lines treated with vehicle control or 25 ng/ml IL-1α or IL-1β for 5 days. MDA-MB-231, BT549, PC3 and DU145 cell lines were treated with vehicle control only. (A) RT-qPCR shows that CCL20 , CD68 , IL8 , p62 , SOX9 and Zeb1 are induced by IL-1 in MCF7 and/or LNCaP cell lines and are basally high in MDA-MB-231, BT549, PC3 and/or DU145 cell lines. (B) RT-qPCR shows that CXCR7 and MMP16 , but not CDK2 or PLK1 , are downregulated by IL-1 in MCF7 and/or LNCaP cell lines and are basally high in MDA-MB-231, BT549, PC3 and/or DU145 cell lines. N = 3 biological replicates; error bars, +/−STDEV; p-value, * ≤ 0.05, ** ≤ 0.005, ***≤ 0.0005. mRNA fold change is normalized to MCF7 vehicle control for the BCa cell lines and to LNCaP vehicle control for the PCa cell lines.

Article Snippet: Human recombinant IL-1α (GoldBio, St Louis MO; 1110-01A-100) and IL-1β (GoldBio, St Louis MO; 1110-01B-100) were resuspended in 0.1% bovine serum albumin (BSA, Thermo Fisher Scientific; BP 1600-1) in 1X phosphate buffered saline (PBS).

Techniques: Quantitative RT-PCR

Journal: Turkish Journal of Chemistry

Article Title: Synthesis and characterization of new hexahydroquinoline derivatives and evaluation of their cytotoxicity, intracellular ROS production, and inhibitory effects on inflammatory mediators

doi: 10.55730/1300-0527.3686

Figure Lengend Snippet: Inflammation markers and and complement protein levels in the study groups. IL-1α: interleukin-1-alpha; IL-10: interleukin-10; TGF-β1: transforming growth factor-1-beta; TNF-α: tumor necrosis factor-alpha; C3: complement C3; C9: complement C9.

Article Snippet: TGF-β1, IL-1a, IL-10, TNF-α, C3, and C9 kits were purchased from BT Lab (Birmingham, UK).

Techniques: