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Image Search Results
Journal: Redox Biology
Article Title: Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin
doi: 10.1016/j.redox.2021.102042
Figure Lengend Snippet: Topical HOCl modulates inflammatory and redox stress response gene expression in UV-induced SKH-1 high-risk mouse skin . (a) At the end of the murine photocarcinogenesis experiment (as detailed in ), dorsal skin was harvested followed by mRNA preparation and Oxidative Stress Plus RT 2 Profiler™ PCR Array analysis: Differential gene expression as depicted by volcano plot [UV-induced high-risk skin treated with ‘HOCl in carrier’ (group 2) versus ‘carrier only’ (group 1); cut-off line: expression differential ≥ 2; p value ≤ 0.05; red dots: upregulated; green dots: downregulated). (b) Tabular summary of statistically significant expression changes. (c) Single RT-qPCR assessment of gene expression ( Il19 , Ptg2 , Tlr4 , Txnip ). (d,e) At the end of the experiment, FFPE-skin sections (5 μm) were analyzed by IHC (H&E, COX-2, IL-19, Ki67) with hematoxylin counterstaining. (d) Tumor cross-sections (‘group 1’ specimens) displaying representative IHC stains; scale bar: 100 μm. (e) Comparative IHC expression analysis of specimens (group 1 versus group 2; scale bar: 100 μm) with summary quantification (bar graphs: H-score) including epidermal thickness (μm), IL-19, COX-2, Ki67, and 3-Cl-Tyr. For bar graphs comparing two groups only, statistical significance was calculated employing the Student's two-tailed t -test (*p < 0.05; **p < 0.01; ***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: Murine 20X primer/probes [ Nos2 (Mm_00440502_m1), Ptgs2 (Mm_00478374_m1), Txnip (Mm_01265659_g1), Tlr4 (Mm_00445273_m1),
Techniques: Gene Expression, Expressing, Quantitative RT-PCR, Two Tailed Test