Journal: Journal of Oncology
Article Title: Expression Analysis of the Mediators of Epithelial to Mesenchymal Transition and Early Risk Assessment of Therapeutic Failure in Laryngeal Carcinoma
Figure Lengend Snippet: IL-10 expression supports EMT in invasive LSCC. (a) IL-10 plasmatic concentrations by ELISA in healthy donors (HD) ( n = 9) and LSCC patients ( n = 20), and Mann–Whitney U test performed for statistical analysis; data are shown as mean ± SD, ∗ p ≤ 0.05. (b) Representative IL-10 and CD68 stainings in laryngeal tissues (magnification ×40) (yellow arrow: normal epithelium, black arrow: tumor cells, and green arrow: stomal cells). (c) Percentage of stromal cells producing IL-10 was evaluated by IHC and correlated to the percentage of CD68 + stromal macrophage by the Pearson correlation test. (d) Correlogram: EMT biomarkers expression levels versus IL-10 and NOS2 expression in invasive LSCC ( n = 20).
Article Snippet: Immunohistochemistry (IHC) Tissue sections of 5 μ m were tested against E-cadherin (NCH-38, prediluted format, Dako), β -catenin (17C2, prediluted format, Novocastra), vimentin (V9, 1/500 Novocastra), NOS2 (SAB4502012, 1/1000, Sigma), NF-κ B (p65, 2A12A7, 1/500, Invitrogen), TGF-β (TB21, 1/1000, Thermofisher), IL-6 (10C12, 1/100, Novocastra), IL-17 (50104, 1/100, Invitrogen), IL-10 (945A2A5, 1/100, Invitrogen), CD68 (KP1, prediluted format, Dako), and MMP-9 (2C3, 1/200, Santa Cruz Biotechnology).
Techniques: Expressing, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Immunohistochemistry