Journal: Parasitology Research
Article Title: Absence of IL-17A in Litomosoides sigmodontis-infected mice influences worm development and drives elevated filarial-specific IFN-γ
Figure Lengend Snippet: Flow cytometry-based differentiation of TC cells revealed comparable monocyte, macrophage, neutrophil and eosinophil numbers. Groups of WT and IL-17A −/− C57BL/6 mice were infected with L. sigmodontis for 28 days. Within the TC, the site of infection, the absolute number of CD11b + SiglecF − Ly6c + monocytes ( a ), CD11b + SiglecF − F4/80 + macrophages ( b ), CD11b + SiglecF − GR1 + neutrophils ( c ) and CD11b + SiglecF + eosinophils ( d ) was determined in individual mice using flow cytometry. Values are expressed as mean ± SEM and symbols show levels in each mouse from one infection experiments ( n = 10 IL-17A −/− and n = 10 WT mice). Statistical significances between the indicated groups were obtained using the Mann-Whitney U tests
Article Snippet: Thereafter, cells were stained with combinations of fluorophores (FITC, PE, PE-Cy7, PerCp-Cy5.5, APC) conjugated with anti-mouse CD4, CD11b, Foxp3, F4/80, GR1, Ly6c, pStat3 (Y705), Rorγt (eBioscience) and IL-17A (Biolegend, San Diego, USA) monoclonal antibodies to determine distinct cell populations.
Techniques: Flow Cytometry, Cytometry, Mouse Assay, Infection, MANN-WHITNEY