Journal:

Article Title: IL-15 plays a major role in the persistence of Tax-specific CD8 cells in HAM/TSP patients

doi: 10.1073/pnas.251540598

Figure Lengend Snippet: The Ki-67 and Annexin V staining of HAM/TSP PBMC after 2- and 6-day cultures. The PBMC from HAM/TSP patients (pt#) were cultured for 2 or 6 days with media alone, anti-IL-2 + anti-Tac, or anti-IL-15 + Mikβ1. The cells were collected and stained with tetramer-Tax, anti-CD8 antibody, and either Ki-67 or Annexin V. The Tax-specific CD8 cells were gated and analyzed for Annexin V or Ki-67 staining. (A) The Ki-67 staining of Tax-specific CD8 cells in day 2 and day 6 is reported as the percentage of the Tax-specific CD8 cells that were positive for Ki-67 relative to the total Tax-specific CD8 cells. (B) The Annexin V staining of Tax-specific CD8 cells in day 2 and day 6 is reported as the percentage of the Tax-specific CD8 cells that were positive for Annexin V staining at day 2 or day 6 relative to the total number of Tax-specific CD8 cells.

Article Snippet: The antibodies used in this study include: UPC10, which is a control murine IgG2a Ig (Sigma), anti-IL-2 antibody (a neutralizing monoclonal anti-IL-2 antibody, R & D Systems), a neutralizing anti-IL-15 antibody (R & D Systems), an IL-2-blocking anti-IL-2Rα antibody, anti-Tac (Metabolism Branch, National Cancer Institute, National Institutes of Health) ( 30 ), or an anti-IL-2/15Rβ antibody, Mikβ1, which blocks IL-15 but not IL-2 action on T cells (a gift from Mitsuru Tsudo, Tokyo Metropolitan Institute of Medical Sciences) ( 31 ).

Techniques: Staining, Cell Culture