Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Type I IFN-producing CD4 Valpha14i NKT cells facilitate priming of IL-10-producing CD8 T cells by hepatocytes.

doi: 10.4049/jimmunol.178.4.2083

Figure Lengend Snippet: FIGURE 6. The cytokine response of OT-I CD8 T cells primed by HC with or without NKT cell help. A, OT-I CD8 T cells (105/well) were cocul- tured with GalCer- and SIINFEKL peptide-pulsed HC (104/well) with () or without () NKT cells (4 104/well). Cells were harvested after a 72-h culture, incubated for 4 h with ionomycin (750 ng/ml) and PMA (50 ng/ml), washed, and tested for IL-10 secretion in a 3-h capture assay. Cells were labeled with a PE-conjugated anti-IL-10 mAb and an anti-CD8 mAb. Data from a representative of five independent experiments are shown. B, OT-I CD8 T cells (105/well) were cocultured for 72 h with GalCer-/ SIINFEKL peptide-pulsed HC (104/well) with () or without () NKT cells (4 104/well). CD8 T blasts were harvested, purified, and restimu- lated in vitro for 48 h with peptide-pulsed DC. IFN-, IL-2, and IL-10 in 48-h supernatants of these secondary cultures were determined by ELISA. Mean values of triplicates (SEM) of a representative of four independent experiments are shown.

Article Snippet: IL-10-producing CD8 T cells were identified by a cytokine capture assay (catalog no. 130-090-489; Miltenyi Biotec) combined with surface staining with the allophycocyanin-conjugated anti-CD8 mAb 53-6.7 (catalog no. 553035; BD Biosciences).

Techniques: Incubation, Labeling, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Histology and IL-2 expression in rat hemorrhoidal tissue. A Microscopic appearance of the hemorrhoid areas of the rats in each group (HE, ×50; a model group, b control group). B Expression of IL-2 in anorectal tissue in each group detected by immunohistochemical staining. (IHC, ×50; a model group, b control group)

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Expressing, Control, Immunohistochemical staining, Staining

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Immunohistochemical overview of anorectal cytokine expression. Expression of A IL-2, B IL-6, C , and D TNFα in anorectal tissue in each group detected by immunohistochemical staining (IHC, ×50; a model group, b control group)

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Immunohistochemical staining, Expressing, Staining, Control

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Relative mRNA expression of pro-inflammatory cytokines A IL-2, B IL-6, and C TNFα in anorectal tissue in each group. * P < 0.05 vs control group

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Expressing, Control

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Western blotting for detecting protein expression of IL-2, IL-6, and TNFα

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Western Blot, Expressing

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: IL-2, IL-6, and TNFα protein levels in anorectal tissue are significantly elevated in the model group compared to the control group (* P < 0.05). A IL-2, B IL-6, and C TNFα protein expression in anorectal tissue. * P < 0.05 vs control group

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Control, Expressing

Journal: Scientific Reports

Article Title: CXCL8 mediates macrophage migration in canine oral malignant melanoma

doi: 10.1038/s41598-025-22749-x

Figure Lengend Snippet: mRNA expression levels of chemokine in seven canine melanoma cell lines. a CCL5 , b CX3CL1 , c CSF-1 , d IL-34 , ( e ) CXCL8 , and ( f ) CCL2 . The relative expression level was normalized to the RPL32 gene . g Correlation between the number of migratory RAW264.7 and CXCL8 mRNA expression levels. h Production of CXCL8 in seven canine melanoma cell lines. i Correlation between the number of migratory RAW264.7 and CXCL8 secretion. All data are presented as means ± SD of three independent experiments.

Article Snippet: The recombinant canine CXCL8 protein (NBP2-34,908, Novus Biologicals, Centennial, CO, USA) was supplemented at a final concentration of 200 ng/mL into the CM.

Techniques: Expressing

Journal: Scientific Reports

Article Title: CXCL8 mediates macrophage migration in canine oral malignant melanoma

doi: 10.1038/s41598-025-22749-x

Figure Lengend Snippet: a , b Relative number of RAW264.7 cells migrated by CMeC1 wild-type CM or CMeC1 CXCL8 knockout CM. c Relative number of RAW264.7 cells migrated by CMeC1 CM. The CM of CMeC1 wild-type cells were treated with anti-dog CXCL8 (1 μg/ml) or mouse IgG isotype (1 μg/ml). All data are presented as means ± SD of three independent experiments. ** P < 0.01; *** P < 0.001.

Article Snippet: The recombinant canine CXCL8 protein (NBP2-34,908, Novus Biologicals, Centennial, CO, USA) was supplemented at a final concentration of 200 ng/mL into the CM.

Techniques: Knock-Out

Journal: Scientific Reports

Article Title: CXCL8 mediates macrophage migration in canine oral malignant melanoma

doi: 10.1038/s41598-025-22749-x

Figure Lengend Snippet: Relative number of RAW264.7 cells migrated by CMeC1 CM. The CM of CMeC1 wild-type or CXCL8 knockout cells were treated with dog CXCL8 recombinant protein (200 ng/ml). All data are presented as means ± SD of three independent experiments. ** P < 0.01; *** P < 0.001.

Article Snippet: The recombinant canine CXCL8 protein (NBP2-34,908, Novus Biologicals, Centennial, CO, USA) was supplemented at a final concentration of 200 ng/mL into the CM.

Techniques: Knock-Out, Recombinant

Journal: Scientific Reports

Article Title: CXCL8 mediates macrophage migration in canine oral malignant melanoma

doi: 10.1038/s41598-025-22749-x

Figure Lengend Snippet: Relative number of canine macrophage DH82 cells migrated by CMeC1 CM. The CM of CMeC1 wild-type or CXCL8 knockout were treated with anti-dog CXCL8 (1 μg/ml) or mouse IgG isotype (1 μg/ml). All data are presented as means ± SD of four independent experiments. ** P < 0.01; *** P < 0.001.

Article Snippet: The recombinant canine CXCL8 protein (NBP2-34,908, Novus Biologicals, Centennial, CO, USA) was supplemented at a final concentration of 200 ng/mL into the CM.

Techniques: Knock-Out

Journal: Journal of nanobiotechnology

Article Title: Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

doi: 10.1186/s12951-025-03241-0

Figure Lengend Snippet: Fig. 3 Selection and Expression of Core M-DEGs in the GEO Dataset. Note: (A) LASSO selection plot for M-DEGs; (B) SVM-RFE analysis results for M-DEGs; (C) Results from the Random Forest algorithm for M-DEGs; (D) Venn diagram showing the intersection of results from LASSO, SVM-RFE, and Random For est algorithms; (E) Expression levels of IL4I1 in NP samples from each group (n = 6) in the merged dataset; (F) Pseudo-time gene expression curve of IL4I1 in MΦ subtypes from scRNA-seq data, with time on the x-axis and gene expression level on the y-axis; ** P < 0.01 compared between the two groups

Article Snippet: The concentration of IL4I1 in IL4I1-NPs and IL4I1MNPs was determined using a human IL4I1 EnzymeLinked Immunosorbent Assay (ELISA) kit (NOVUS, NBP3-06773) according to the manufacturer’s instructions.

Techniques: Selection, Expressing, Gene Expression

Journal: Journal of nanobiotechnology

Article Title: Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

doi: 10.1186/s12951-025-03241-0

Figure Lengend Snippet: Fig. 4 Influence of IL4I1 on M1-M2 Polarization of MΦs. Note: (A) Schematic representation of IL4I1 treatment on MΦ induced M1 or M2 polarization; (B) RT-qPCR analysis of expression of M1 and M2 MΦ marker genes in different groups of MΦ; (C) Representative immunofluorescence images of MΦ in differ ent groups (scale bar = 25 μm), quantification of fluorescence intensities of CD80 and CD86, CD163 and CD206 co-localization; (D) Levels of IL-1β, TNF-α, TGF-β, and IL-10 in the supernatant of cultured MΦ in each group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, all experiments were repeated thrice

Article Snippet: The concentration of IL4I1 in IL4I1-NPs and IL4I1MNPs was determined using a human IL4I1 EnzymeLinked Immunosorbent Assay (ELISA) kit (NOVUS, NBP3-06773) according to the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Marker, Immunofluorescence, Fluorescence, Cell Culture

Journal: Journal of nanobiotechnology

Article Title: Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

doi: 10.1186/s12951-025-03241-0

Figure Lengend Snippet: Fig. 5 Preparation, characterization, and In Vitro Uptake of IL4I1-NPs and IL4I1-MNPs. Note: (A) Schematic depiction of the preparation process for IL4I1-NPs and IL4I1-MNPs; (B) Representative images of IL4I1-NPs and IL4I1-MNPs using TEM (scale bar = 200 nm); (C-D) DLS analysis of the size and zeta potential of IL4I1-NPs and IL4I1-MNPs; (E) SDS-PAGE protein analysis of cell lysates, MΦCM, IL4I1-NPs, and IL4I1-MNPs; (F) Loading capacity of IL4I1 in IL4I1-NPs and IL4I1-MNPs; (G) The loading capacity and encapsulation efficiency of IL4I1 within IL4I1-NPs and IL4I1-MNPs, respectively, with monitoring of encapsulation rates within 72 h at 4 °C; (H) In vitro cumulative release curve of IL4I1 from IL4I1-NPs and IL4I1-MNPs in PBS at 37 °C; (I) Particle size detec tion of IL4I1-MNPs in deionized water, 1×PBS, and 50% FBS over three days; (J) Schematic diagram of the preparation process for DiR-NPs and DiR-MNPs; (K) Flow cytometry analysis of fluorescence intensity of DiR in MΦ after incubation with DiR-NPs and DiR-MNPs for 1, 2, and 4 h; (L) Representative images (scale bar = 15 μm) and quantitative analysis of fluorescence intensity of MΦ uptake of DiR-NPs and DiR-MNPs; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, all experiments were conducted in triplicate

Article Snippet: The concentration of IL4I1 in IL4I1-NPs and IL4I1MNPs was determined using a human IL4I1 EnzymeLinked Immunosorbent Assay (ELISA) kit (NOVUS, NBP3-06773) according to the manufacturer’s instructions.

Techniques: In Vitro, Zeta Potential Analyzer, SDS Page, Encapsulation, Flow Cytometry, Fluorescence, Incubation

Journal: Journal of nanobiotechnology

Article Title: Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

doi: 10.1186/s12951-025-03241-0

Figure Lengend Snippet: Fig. 6 Impact of IL4I1-MNPs on MΦ Polarization in IDD Mice In Vivo. Note: (A) RT-qPCR analysis of expression of M1 and M2 MΦ marker genes in IVD MΦs of mice in different groups (n = 6); (B) Percentage of M1 MΦs (CD80+CD86+) in IVD MΦs of mice in different groups (n = 6); (C) Percentage of M2 MΦs (CD163+CD206+) in IVD MΦs of mice in different groups (n = 6); (D) ELISA detection of IL-1β, TNF-α, TGF-β, and IL-10 levels in IVD of mice in different groups (n = 6); * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: The concentration of IL4I1 in IL4I1-NPs and IL4I1MNPs was determined using a human IL4I1 EnzymeLinked Immunosorbent Assay (ELISA) kit (NOVUS, NBP3-06773) according to the manufacturer’s instructions.

Techniques: In Vivo, Quantitative RT-PCR, Expressing, Marker, Enzyme-linked Immunosorbent Assay

Journal: Journal of nanobiotechnology

Article Title: Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

doi: 10.1186/s12951-025-03241-0

Figure Lengend Snippet: Fig. 8 Exploration of the Role of FGR in Regulating MΦ Polarization Balance Mediated by IL4I1-MNPs. Note: (A) Illustration of FGR-OE transfection; (B) RT- qPCR analysis of FGR expression in different groups of MΦ; (C) Schematic representation of MΦ polarization induction post-NC-OE or FGR-OE transfection, followed by treatment with PBS or IL4I1-MNPs; (D) RT-qPCR analysis of expression of M1 and M2 MΦ marker genes in different groups of MΦ; (E) Repre sentative immunofluorescence images of MΦ in different groups (scale bar = 25 μm), quantification of CD80 and CD86, CD163 and CD206 co-localization fluorescence intensity; (F) Levels of IL-1β, TNF-α, TGF-β, and IL-10 in the supernatant of cultured MΦ in different groups; *P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, all experiments were repeated three times

Article Snippet: The concentration of IL4I1 in IL4I1-NPs and IL4I1MNPs was determined using a human IL4I1 EnzymeLinked Immunosorbent Assay (ELISA) kit (NOVUS, NBP3-06773) according to the manufacturer’s instructions.

Techniques: Transfection, Quantitative RT-PCR, Expressing, Marker, Immunofluorescence, Fluorescence, Cell Culture

Journal: Journal of nanobiotechnology

Article Title: Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

doi: 10.1186/s12951-025-03241-0

Figure Lengend Snippet: Fig. 9 Effect of CHG@IL4I1-MNPs on MΦ Polarization in IDD Mice. Note: (A) RT-qPCR analysis of the expression of M1 and M2 MΦ marker genes in IVD MΦs of mice (n = 6); (B) Percentage of M1 MΦs in IVD MΦs of mice (n = 6); (C) Percentage of M2 MΦs in IVD MΦs of mice (n = 6); (D) ELISA detection of IL- 1β, TNF-α, TGF-β, and IL-10 levels in the IVD of mice (n = 6); n.s. P > 0.05 for comparison between groups, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: The concentration of IL4I1 in IL4I1-NPs and IL4I1MNPs was determined using a human IL4I1 EnzymeLinked Immunosorbent Assay (ELISA) kit (NOVUS, NBP3-06773) according to the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Marker, Enzyme-linked Immunosorbent Assay, Comparison