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Image Search Results
Journal: Journal of Functional Foods
Article Title: Salidroside alleviates UVB-induced skin damage by inhibiting keratinocytes pyroptosis via the AQP3/ROS/GSDMD-N signaling pathway
doi: 10.1016/j.jff.2023.105647
Figure Lengend Snippet: Fig. 4. Salidroside (SAL) alleviates UVB induced inflammasome activation and reduces the release of inflammatory factors. (A) SAL inhibited the expressions of NLRP3, Caspase1, C-Caspase1 and GSDMD-N. (B) Concentration of IL-1β in supernatants of the culture media. (C) Concentration of IL-18 in supernatants of the culture media. All data were displayed as mean ± SEM, n = 5. *P < 0.05, **P < 0.01 versus group of UVB.
Article Snippet: The followings are the information of primary antibodies: rabbit anti-NLRP3 (Q96P20) mAb T55651 (1:1000, Abmart), rabbit anti-Caspase-1 (E9R2D) mAb #83383S (1:1000, CST), rabbit anti- C-Caspase-1 (Asp296) (E2G2I) Rabbit mAb #89332 (reactivity: mouse) (1:1000, CST), anti-C-Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199(reactivity: human) (1:1000,CST), anti- Gasdermin D N terminal (P57764) (Q9D8T2) Rabbit mAb, PU224937(1:1000, abmart),
Techniques: Activation Assay, Concentration Assay
Journal: Journal of Functional Foods
Article Title: Salidroside alleviates UVB-induced skin damage by inhibiting keratinocytes pyroptosis via the AQP3/ROS/GSDMD-N signaling pathway
doi: 10.1016/j.jff.2023.105647
Figure Lengend Snippet: Fig. 5. Salidroside (SAL) suppresses pyroptosis via down-regulatin AQP3/ROS/GSDMD-N signaling pathway in HaCaT cell line. (A) The bands and analysis results of AQP3 expression in HaCaT cell line after the treatment of SAL. (B) The fluorescence results of the HaCaT cells with overexpression of AQP3. (C) The bands and analysis of AQP3 expression in HaCaT cell line after overexpression. (D) qPCR results of HaCaT with AQP3 overexpression and NC. (E) The concentration of H2O2 in cells. (F) The bands and analysis of Caspase1, C-Caspase1, NLRP3 and GSDMD-N after the overexpression of AQP3. (G) The concentration of IL-18 in cells after the overexpression of AQP3. (H) The concentration of IL-1β in cells after the overexpression of AQP3. (I) The level of ROS in cells after the overexpression of AQP3. (J) The inflammasomes in cells visualized by TEM. All data were displayed as mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus group of UVB; # P < 0.05, ##P < 0.01 versus group of AQP3+/+.
Article Snippet: The followings are the information of primary antibodies: rabbit anti-NLRP3 (Q96P20) mAb T55651 (1:1000, Abmart), rabbit anti-Caspase-1 (E9R2D) mAb #83383S (1:1000, CST), rabbit anti- C-Caspase-1 (Asp296) (E2G2I) Rabbit mAb #89332 (reactivity: mouse) (1:1000, CST), anti-C-Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199(reactivity: human) (1:1000,CST), anti- Gasdermin D N terminal (P57764) (Q9D8T2) Rabbit mAb, PU224937(1:1000, abmart),
Techniques: Expressing, Fluorescence, Over Expression, Concentration Assay
Journal: Journal of Functional Foods
Article Title: Salidroside alleviates UVB-induced skin damage by inhibiting keratinocytes pyroptosis via the AQP3/ROS/GSDMD-N signaling pathway
doi: 10.1016/j.jff.2023.105647
Figure Lengend Snippet: Fig. 7. Salidroside (SAL) relieves UVB-induced oxidative damage and pyroptosis in mice (A) Immunohistochemical staining and analysis for AQP3. (B) The expression of AQP3 in the skin leision was dettected by Western blotting. (C) The concentrations of H2O2. (D) MDA, (E F) SOD, and GSH in skin lesions. (G) Immunohistochemistry staining and analysis for NLRP3. (H) The expressions of NLRP3, Caspase-1, C-Caspase-1, GSDMD-N, IL-1β and IL-18 were detected by Western blotting. All data were displayed as Mean ± SD, n = 3. *P < 0.05, **P < 0.01 versus group of UVB.
Article Snippet: The followings are the information of primary antibodies: rabbit anti-NLRP3 (Q96P20) mAb T55651 (1:1000, Abmart), rabbit anti-Caspase-1 (E9R2D) mAb #83383S (1:1000, CST), rabbit anti- C-Caspase-1 (Asp296) (E2G2I) Rabbit mAb #89332 (reactivity: mouse) (1:1000, CST), anti-C-Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199(reactivity: human) (1:1000,CST), anti- Gasdermin D N terminal (P57764) (Q9D8T2) Rabbit mAb, PU224937(1:1000, abmart),
Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot, Immunohistochemistry