Journal: Carcinogenesis

Article Title: Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2.

doi: 10.1093/carcin/23.9.1511

Figure Lengend Snippet: Fig. 4. (A) CSC induces degradation of IκBα. Two million U937 cells per ml were treated with either 0.1 nM TNF or 10 µg/ml CSC for different times. The cytoplasmic extracts were prepared and assayed for IκBα by western blot using IκBα-specific antibodies. (B) CSC activates IκBα kinase. Two million U937 cells per milliliter were treated with either 0.1 nM TNF or with 25 µg/ml CSC for different times. Thereafter the cytoplasmic extracts were prepared and assayed for IKK by the immunocomplex kinase assay (upper panel) and for IKKα (middle panel) and IKKβ (lower panel) protein by western blot analysis as described in Materials and methods.

Article Snippet: Monoclonal antibodies to IKKα and IKKβ were kindly provided by Imgenex (San Diego, CA) and antibodies against COX-2 were obtained from Transduction Laboratories (Lexington, KY).

Techniques: Western Blot, Kinase Assay

Journal: International Journal of Molecular Sciences

Article Title: Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells

doi: 10.3390/ijms20225730

Figure Lengend Snippet: List of the names, sources, companies, molecular weight and dilutions of all the antibodies that were used for Western blots.

Article Snippet: IKBKG , 38 , 1:500 , Origene #TA812460 , MS.

Techniques: Molecular Weight, Western Blot