Journal: Vaccines

Article Title: Protective Efficacy Induced by the Common Eimeria Antigen Elongation Factor 2 against Challenge with Three Eimeria Species in Chickens.

doi: 10.3390/vaccines12010018

Figure Lengend Snippet: Figure 4. The proportion of the T cell subpopulation in chickens immunized with PBS, pET-32a tag protein and rEmEF2 was determined by flow cytometry 7 days after the first and second immu- nization. (A) Detection of CD3+CD4+ T lymphocytes in immunized chickens 7 days after the first immunization. (B) Detection of CD3+CD4+ T lymphocytes in immunized chickens 7 days after the second immunization. (C) Detection of CD3+CD8+ T lymphocytes in immunized chickens 7 days after the first immunization. (D) Detection of CD3+CD8+ T lymphocytes in immunized chickens 7 days after the second immunization. 1: PBS control group. 2: pET-32a tag protein control group. 3: rEmEF2 group.

Article Snippet: Whereafter, CD3 mouse anti-chicken FITC antibody (Southern Biotechnology Associates, Birmingham, AL, USA), CD4 mouse anti-chicken PE antibody (Southern Biotechnology Associates, Birmingham, AL, USA) and CD8 mouse anti-chicken PE antibody (Southern Biotechnology Associates, Birmingham, AL, USA) were used to detect the T cell subpopulations with a FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Flow Cytometry, Control

Journal: PLoS ONE

Article Title: Investigations of a Rabbit ( Oryctolagus cuniculus ) Model of Systemic Lupus Erythematosus (SLE), BAFF and Its Receptors

doi: 10.1371/journal.pone.0008494

Figure Lengend Snippet: A. PBMCs were gated based on forward and side scatter (FSCxSSC) profiles (left panel). IgM + cells in peripheral blood can be divided into IgM high and IgM low cell populations (right panel). B. Representative plots from pre-immune (Pre) and post 3 rd boost (>3B) analyses of GR anti-dsDNA high responder (GR72) and low responder (GR84) rabbits. The histograms show the staining of total PBMC for BAFF and BR3 and the control biotin-labeled normal goat IgG for BAFF normal goat IgG for BR3 (shaded). C. Representative dot plots from GR anti-dsDNA high responder (GR72) and low responder (GR84). The plots showed the staining of BAFF and TACI on the surface of peripheral blood IgM + cells and of BAFF, TACI and BR3 on CD14 + cells.

Article Snippet: Also used were FITC-labeled mouse anti-rabbit CD14 (clone K4) (Antigenix, America, Inc.), FITC-labeled goat anti-rabbit IgM (Southern Biotechnology Associates), biotin-labeled donkey anti-goat IgG, biotin-labeled goat IgG, normal goat IgG (Jackson ImmunoResearch Laboratories, Inc), and FITC-labeled mouse IgG2a (BD Pharmingen).

Techniques: Staining, Labeling

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Plant-Derived Chalcone 2,2′,5′-Trihydroxychalcone Provides Neuroprotection against Toll-Like Receptor 4 Triggered Inflammation in Microglia

doi: 10.1155/2016/6301712

Figure Lengend Snippet: 225THC treatment modulates inflammatory cell surface marker expression in LPS-treated BV2 microglia. Flow cytometric analysis of 225THC-treated BV2 microglia following LPS stimulation. (a) Representative histogram plots of BV2 cell surface CD11b, CD40, CD54, and CD68 expression following 2 μ g/mL LPS-treatment for 48 hours (unfilled histograms), versus isotype control (black-filled histograms), and in the presence of 10 μ M 225THC (grey-filled histograms). (b) Summary data showing fold changes in CD11b, CD40, CD54, or CD68 expression by mean fluorescence intensity (MFI) from 4 independent experiments. Data are mean values ± SEM where ∗ indicates p < 0.05 by Student's t -test.

Article Snippet: Cells were stained with anti-mouse CD11b-FITC, CD40-FITC, CD54-FITC, and CD68-FITC, or appropriate FITC-conjugated isotype control antibodies (Miltenyi Biotec, UK) as per the manufacturer's instructions and cell surface staining was assessed by flow cytometry (FACSCalibur running CellQuest Pro; Becton Dickinson, UK) and analysed using Flowing software v2.5.1.

Techniques: Marker, Expressing, Control, Fluorescence