igm antibodies Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher igm
    Tet2 deletion in hematopoietic cells induces GC hyperplasia. A, Representative flow cytometry plot and quantification of <t>(B220</t> + CD95 + GL7 + ) GC B-cells from Vav-Cre/ Tet2 +/+ (n=5) and Vav-Cre/ Tet2 −/− (n=5) mice at day 10 after immunization with SRBC. B , Quantification of flow cytometry data corresponding to total B cells (B220 + ), mature B cells (B220 + IgD + <t>IgM</t> + ), transitional B cells (B220 + IgD int IgM + ), follicular B cells (B220 + CD23 + CD21 + ), marginal zone B cells (B220 + CD23 low CD21 + ) and plasmablasts/PC (CD138 + ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. C , Representative histologic sections of formalin-fixed, paraffin-embedded spleens from Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. Sections were stained with H E and antibodies specific for B220, PNA and Ki-67. D , E , Quantification of GC area ( D ) and number of GCs ( E ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. two-tailed t test, ***p
    Igm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm/product/Thermo Fisher
    Average 99 stars, based on 4205 article reviews
    Price from $9.99 to $1999.99
    igm - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Millipore polyclonal antibodies
    Tet2 deletion in hematopoietic cells induces GC hyperplasia. A, Representative flow cytometry plot and quantification of <t>(B220</t> + CD95 + GL7 + ) GC B-cells from Vav-Cre/ Tet2 +/+ (n=5) and Vav-Cre/ Tet2 −/− (n=5) mice at day 10 after immunization with SRBC. B , Quantification of flow cytometry data corresponding to total B cells (B220 + ), mature B cells (B220 + IgD + <t>IgM</t> + ), transitional B cells (B220 + IgD int IgM + ), follicular B cells (B220 + CD23 + CD21 + ), marginal zone B cells (B220 + CD23 low CD21 + ) and plasmablasts/PC (CD138 + ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. C , Representative histologic sections of formalin-fixed, paraffin-embedded spleens from Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. Sections were stained with H E and antibodies specific for B220, PNA and Ki-67. D , E , Quantification of GC area ( D ) and number of GCs ( E ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. two-tailed t test, ***p
    Polyclonal Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibodies/product/Millipore
    Average 99 stars, based on 3635 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibodies - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher goat anti mouse igm heavy chain secondary antibody
    Tet2 deletion in hematopoietic cells induces GC hyperplasia. A, Representative flow cytometry plot and quantification of <t>(B220</t> + CD95 + GL7 + ) GC B-cells from Vav-Cre/ Tet2 +/+ (n=5) and Vav-Cre/ Tet2 −/− (n=5) mice at day 10 after immunization with SRBC. B , Quantification of flow cytometry data corresponding to total B cells (B220 + ), mature B cells (B220 + IgD + <t>IgM</t> + ), transitional B cells (B220 + IgD int IgM + ), follicular B cells (B220 + CD23 + CD21 + ), marginal zone B cells (B220 + CD23 low CD21 + ) and plasmablasts/PC (CD138 + ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. C , Representative histologic sections of formalin-fixed, paraffin-embedded spleens from Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. Sections were stained with H E and antibodies specific for B220, PNA and Ki-67. D , E , Quantification of GC area ( D ) and number of GCs ( E ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. two-tailed t test, ***p
    Goat Anti Mouse Igm Heavy Chain Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igm heavy chain secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 272 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igm heavy chain secondary antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    95
    SouthernBiotech anti igm
    SVP[Rapa] treatment enables AAV8 re-administration in nonhuman primates. a Protocol outline. Male naive cynomolgus monkeys were treated i.v. with 2 × 10 12 vg kg −1 of AAV8-Gaa vector and either SVP[Rapa] (3 mg kg −1 , n = 2, SVP[Rapa]#1 and SVP[Rapa]#2) or SVP[empty] control ( n = 1) and then challenged i.v. on day 30 with 2 × 10 12 vg kg − 1 of AAV8-hF.IX vector and either SVP[Rapa] or SVP[empty] control, as described above. b , c Analysis of b anti-AAV8 <t>IgG</t> antibodies and c anti-AAV8 <t>IgM</t> antibody responses measured by ELISA. d Analysis of anti-AAV8 neutralizing antibodies (NAb) measured with a cell-based neutralization assay. e Analysis of anti-AAV8 IgG secreting B cells in splenocytes measured by B ELISpot. Data are shown as individual replicates and the bars represent mean ± s.d. The dotted line indicates the threshold for positivity corresponding to 50 spot forming units (SFU) per million cells. f Plasma hF.IX antigen levels quantified by ELISA at the indicated time points following administration of AAV8-hF.IX vector. g AAV8-hF.IX vector genome copy number (VGCN) per diploid genome in liver. The symbols represent individual liver lobes (left, right, caudate and quadrate) and the bars represent the mean ± s.d. (4 liver lobes per monkey; one-way ANOVA with Tukey post hoc test, ** p
    Anti Igm, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 610 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti igm/product/SouthernBiotech
    Average 95 stars, based on 610 article reviews
    Price from $9.99 to $1999.99
    anti igm - by Bioz Stars, 2021-01
    95/100 stars
      Buy from Supplier

    94
    Novus Biologicals rabbit polyclonal antibody
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    Rabbit Polyclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody/product/Novus Biologicals
    Average 94 stars, based on 944 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibody - by Bioz Stars, 2021-01
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher goat anti mouse igm heavy chain cross adsorbed secondary antibody
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    Goat Anti Mouse Igm Heavy Chain Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igm heavy chain cross adsorbed secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 534 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igm heavy chain cross adsorbed secondary antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher f ab 2 goat anti human igg iga igm secondary antibody
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    F Ab 2 Goat Anti Human Igg Iga Igm Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/f ab 2 goat anti human igg iga igm secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    f ab 2 goat anti human igg iga igm secondary antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Millipore anti human igm mu chain specific f ab 2 fragment peroxidase antibody
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    Anti Human Igm Mu Chain Specific F Ab 2 Fragment Peroxidase Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human igm mu chain specific f ab 2 fragment peroxidase antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human igm mu chain specific f ab 2 fragment peroxidase antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher peroxidase conjugated anti rabbit
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    Peroxidase Conjugated Anti Rabbit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated anti rabbit/product/Thermo Fisher
    Average 99 stars, based on 547 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated anti rabbit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad goat anti rabbit secondary ab
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    Goat Anti Rabbit Secondary Ab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit secondary ab/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit secondary ab - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher rat monoclonal antibody
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    Rat Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat monoclonal antibody/product/Thermo Fisher
    Average 99 stars, based on 413 article reviews
    Price from $9.99 to $1999.99
    rat monoclonal antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Millipore isotype control antibody
    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit <t>polyclonal</t> human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.
    Isotype Control Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isotype control antibody/product/Millipore
    Average 99 stars, based on 571 article reviews
    Price from $9.99 to $1999.99
    isotype control antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher igm antibody
    Co-localization of PAB-reactive PLTA antigen, <t>IgA,</t> <t>IgM,</t> and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).
    Igm Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm antibody/product/Thermo Fisher
    Average 99 stars, based on 104 article reviews
    Price from $9.99 to $1999.99
    igm antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    SouthernBiotech igm antibodies
    Co-localization of PAB-reactive PLTA antigen, <t>IgA,</t> <t>IgM,</t> and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).
    Igm Antibodies, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 99/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm antibodies/product/SouthernBiotech
    Average 99 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    igm antibodies - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    R&D Systems goat polyclonal antibody
    Co-localization of PAB-reactive PLTA antigen, <t>IgA,</t> <t>IgM,</t> and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).
    Goat Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal antibody/product/R&D Systems
    Average 99 stars, based on 770 article reviews
    Price from $9.99 to $1999.99
    goat polyclonal antibody - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    N/A
    Immunoglobulin M IgM Antibody Biotin is an antibody conjugated to Biotin against Immunoglobulin M IgM
      Buy from Supplier

    N/A
    Polyclonal Antibody to Immunoglobulin M IgM
      Buy from Supplier

    N/A
    Immunoglobulin M IgM Antibody FITC is an antibody conjugated to FITC against Immunoglobulin M IgM
      Buy from Supplier


    Image Search Results


    Tet2 deletion in hematopoietic cells induces GC hyperplasia. A, Representative flow cytometry plot and quantification of (B220 + CD95 + GL7 + ) GC B-cells from Vav-Cre/ Tet2 +/+ (n=5) and Vav-Cre/ Tet2 −/− (n=5) mice at day 10 after immunization with SRBC. B , Quantification of flow cytometry data corresponding to total B cells (B220 + ), mature B cells (B220 + IgD + IgM + ), transitional B cells (B220 + IgD int IgM + ), follicular B cells (B220 + CD23 + CD21 + ), marginal zone B cells (B220 + CD23 low CD21 + ) and plasmablasts/PC (CD138 + ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. C , Representative histologic sections of formalin-fixed, paraffin-embedded spleens from Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. Sections were stained with H E and antibodies specific for B220, PNA and Ki-67. D , E , Quantification of GC area ( D ) and number of GCs ( E ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. two-tailed t test, ***p

    Journal: Cancer discovery

    Article Title: TET2 deficiency causes germinal center hyperplasia, impairs plasma cell differentiation and promotes B-cell lymphomagenesis

    doi: 10.1158/2159-8290.CD-18-0657

    Figure Lengend Snippet: Tet2 deletion in hematopoietic cells induces GC hyperplasia. A, Representative flow cytometry plot and quantification of (B220 + CD95 + GL7 + ) GC B-cells from Vav-Cre/ Tet2 +/+ (n=5) and Vav-Cre/ Tet2 −/− (n=5) mice at day 10 after immunization with SRBC. B , Quantification of flow cytometry data corresponding to total B cells (B220 + ), mature B cells (B220 + IgD + IgM + ), transitional B cells (B220 + IgD int IgM + ), follicular B cells (B220 + CD23 + CD21 + ), marginal zone B cells (B220 + CD23 low CD21 + ) and plasmablasts/PC (CD138 + ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. C , Representative histologic sections of formalin-fixed, paraffin-embedded spleens from Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. Sections were stained with H E and antibodies specific for B220, PNA and Ki-67. D , E , Quantification of GC area ( D ) and number of GCs ( E ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. two-tailed t test, ***p

    Article Snippet: Flow cytometry analysis of spleen cell suspensions was performed using the following fluorescent-labeled anti–mouse antibodies: APC-conjugated anti-B220 (BD Biosciences #553092), anti-CD138 (BD Biosciences #558626) and anti-IgM (eBioscience #17–5790-82); PE-conjugated anti-IgD (BD Biosciences #558597), anti-CD23 (eBioscience #5010271) and anti-CD95 (BD Biosciences #554258); PECy7-conjugated anti-CD21 (BioLegend #123420), anti-CD95 (BD Biosciences #557653), anti-GL7 (BD Biosciences # 561530) and anti-CD86 (BD Biosciences #560582); PEVio770-conjugated anti-B220 (Miltenyi Biotec #130–102-308); FITC-conjugated anti-CXCR4 (BD Biosciences #551967) and anti-IgG1 (ebioscience #11–4011-85); Brilliant Violet 421-conjugated anti-CD138 (BioLegend #142507); PerCP-Cy5.5-conjugated anti-GL7 (BioLegend #144610), anti-CD138 (BioLegend #142510) and anti-IgM (BD Biosciences #550881), APC-Cy7-conjugated anti-CD19 (ebioscience #47–0193-82), AlexaFluor647-conjugated anti-BLIMP1 (BD Biosciences #563643).

    Techniques: Flow Cytometry, Mouse Assay, Formalin-fixed Paraffin-Embedded, Staining, Two Tailed Test

    SVP[Rapa] treatment enables AAV8 re-administration in nonhuman primates. a Protocol outline. Male naive cynomolgus monkeys were treated i.v. with 2 × 10 12 vg kg −1 of AAV8-Gaa vector and either SVP[Rapa] (3 mg kg −1 , n = 2, SVP[Rapa]#1 and SVP[Rapa]#2) or SVP[empty] control ( n = 1) and then challenged i.v. on day 30 with 2 × 10 12 vg kg − 1 of AAV8-hF.IX vector and either SVP[Rapa] or SVP[empty] control, as described above. b , c Analysis of b anti-AAV8 IgG antibodies and c anti-AAV8 IgM antibody responses measured by ELISA. d Analysis of anti-AAV8 neutralizing antibodies (NAb) measured with a cell-based neutralization assay. e Analysis of anti-AAV8 IgG secreting B cells in splenocytes measured by B ELISpot. Data are shown as individual replicates and the bars represent mean ± s.d. The dotted line indicates the threshold for positivity corresponding to 50 spot forming units (SFU) per million cells. f Plasma hF.IX antigen levels quantified by ELISA at the indicated time points following administration of AAV8-hF.IX vector. g AAV8-hF.IX vector genome copy number (VGCN) per diploid genome in liver. The symbols represent individual liver lobes (left, right, caudate and quadrate) and the bars represent the mean ± s.d. (4 liver lobes per monkey; one-way ANOVA with Tukey post hoc test, ** p

    Journal: Nature Communications

    Article Title: Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration

    doi: 10.1038/s41467-018-06621-3

    Figure Lengend Snippet: SVP[Rapa] treatment enables AAV8 re-administration in nonhuman primates. a Protocol outline. Male naive cynomolgus monkeys were treated i.v. with 2 × 10 12 vg kg −1 of AAV8-Gaa vector and either SVP[Rapa] (3 mg kg −1 , n = 2, SVP[Rapa]#1 and SVP[Rapa]#2) or SVP[empty] control ( n = 1) and then challenged i.v. on day 30 with 2 × 10 12 vg kg − 1 of AAV8-hF.IX vector and either SVP[Rapa] or SVP[empty] control, as described above. b , c Analysis of b anti-AAV8 IgG antibodies and c anti-AAV8 IgM antibody responses measured by ELISA. d Analysis of anti-AAV8 neutralizing antibodies (NAb) measured with a cell-based neutralization assay. e Analysis of anti-AAV8 IgG secreting B cells in splenocytes measured by B ELISpot. Data are shown as individual replicates and the bars represent mean ± s.d. The dotted line indicates the threshold for positivity corresponding to 50 spot forming units (SFU) per million cells. f Plasma hF.IX antigen levels quantified by ELISA at the indicated time points following administration of AAV8-hF.IX vector. g AAV8-hF.IX vector genome copy number (VGCN) per diploid genome in liver. The symbols represent individual liver lobes (left, right, caudate and quadrate) and the bars represent the mean ± s.d. (4 liver lobes per monkey; one-way ANOVA with Tukey post hoc test, ** p

    Article Snippet: For NHP samples, anti-monkey IgG-HRP (dilution 1:20,000, 43R-IG020HRP, Fitzgerald Industries, Acton, MA) and anti-IgM (dilution 1:5000, 2020-05, Southern Biotech) were used.

    Techniques: Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Neutralization, Enzyme-linked Immunospot

    Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit polyclonal human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.

    Journal: The Journal of Biological Chemistry

    Article Title: Amyloid Precursor-like Protein 2 and Sortilin Do Not Regulate the PCSK9 Convertase-mediated Low Density Lipoprotein Receptor Degradation but Interact with Each Other *

    doi: 10.1074/jbc.M115.647180

    Figure Lengend Snippet: Co-expression of sortilin, APLP2, or both with PCSK9 has no major effect on LDLR degradation. Huh7 cells were transfected with a total of 3 μg using 1 μg of each vector encoding for either a control protein 7B2 (−), sortilin (+), APLP2 (+), or PCSK9 (+), as indicated. After 48 h, lysates were analyzed by Western blotting for expression of the LDLR, sortilin-Myc, APLP2-V5, intracellular pro- and mature-PCSK9-V5, and β-actin. Media were analyzed for secreted endogenous and overexpressed PCSK9-V5 using a rabbit polyclonal human PCSK9 antibody. Quantification of LDLR expression was normalized against that of β-actin. These data are representative of at least 3 different experiments showing similar results.

    Article Snippet: Mouse APLP2 was detected using a rabbit polyclonal antibody kindly provided by Dr. G. Thinakaran (University of Chicago), whereas human APLP2 was detected with either mAb-V5 or a rabbit polyclonal antibody (Novus Biologicals).

    Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot

    Co-localization of PAB-reactive PLTA antigen, IgA, IgM, and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).

    Journal: PLoS ONE

    Article Title: Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis

    doi: 10.1371/journal.pone.0192408

    Figure Lengend Snippet: Co-localization of PAB-reactive PLTA antigen, IgA, IgM, and C3c detected by double fluorescence immunohistochemistry. A: Anti-IgA antibody (red) vs PAB antibody (green) after MT treatment, B: anti-IgM antibody (red) vs PAB antibody (green) after MT treatment, C: anti-IgM antibody (red) vs anti-IgA antibody (green), and D: anti-C3c antibody (red) vs PAB antibody (green) after MT treatment. Many PAB-reactive SRBs were also positive for IgA, IgM, and C3c, showing yellow-colored double-positive signals (A, B, and D, respectively). Both IgA and IgM colocalized with these SRBs, indicated by yellow-colored double-positive signals (C).

    Article Snippet: After the initial incubation, the plates were further incubated for 60 min at 37°C with unlabeled mouse anti-PLTA antibody (PAB) diluted 1:500; unlabeled rabbit anti-human C1q and C3c (DAKO 0136 and A062, respectively) diluted 1:500; or biotinylated goat anti-human IgG, IgA, or IgM antibody (Invitrogen, Carlsbad, CA, USA) diluted 1:5000.

    Techniques: Fluorescence, Immunohistochemistry

    Immuno-electron microscopy images of SRBs in sinus macrophages of sarcoid lymph nodes. A and B: IHC with PAB antibody after MT treatment, C: IHC with anti-IgA antibody, and D: IHC with anti-IgM antibody. Note that dense black-colored reaction products by each antibody were located along the peripheral rim of the SRBs. A similar distribution of PAB-reactivity was observed in a large spherical-shaped HW body (A).

    Journal: PLoS ONE

    Article Title: Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis

    doi: 10.1371/journal.pone.0192408

    Figure Lengend Snippet: Immuno-electron microscopy images of SRBs in sinus macrophages of sarcoid lymph nodes. A and B: IHC with PAB antibody after MT treatment, C: IHC with anti-IgA antibody, and D: IHC with anti-IgM antibody. Note that dense black-colored reaction products by each antibody were located along the peripheral rim of the SRBs. A similar distribution of PAB-reactivity was observed in a large spherical-shaped HW body (A).

    Article Snippet: After the initial incubation, the plates were further incubated for 60 min at 37°C with unlabeled mouse anti-PLTA antibody (PAB) diluted 1:500; unlabeled rabbit anti-human C1q and C3c (DAKO 0136 and A062, respectively) diluted 1:500; or biotinylated goat anti-human IgG, IgA, or IgM antibody (Invitrogen, Carlsbad, CA, USA) diluted 1:5000.

    Techniques: Immuno-Electron Microscopy, Immunohistochemistry

    Insoluble immune complexes in sinus macrophages of control lymph nodes from colon cancer patients. In a representative case of control lymph nodes with IICs from colon cancer patients, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, many IgA-positive and a few IgM-positive small particles were detected (C and D, respectively), although a few PAB-reactive SRBs were detected with no difference in the number between the sections with and without MT treatment (F and E, respectively). Scale bar: 20 μm.

    Journal: PLoS ONE

    Article Title: Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis

    doi: 10.1371/journal.pone.0192408

    Figure Lengend Snippet: Insoluble immune complexes in sinus macrophages of control lymph nodes from colon cancer patients. In a representative case of control lymph nodes with IICs from colon cancer patients, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, many IgA-positive and a few IgM-positive small particles were detected (C and D, respectively), although a few PAB-reactive SRBs were detected with no difference in the number between the sections with and without MT treatment (F and E, respectively). Scale bar: 20 μm.

    Article Snippet: After the initial incubation, the plates were further incubated for 60 min at 37°C with unlabeled mouse anti-PLTA antibody (PAB) diluted 1:500; unlabeled rabbit anti-human C1q and C3c (DAKO 0136 and A062, respectively) diluted 1:500; or biotinylated goat anti-human IgG, IgA, or IgM antibody (Invitrogen, Carlsbad, CA, USA) diluted 1:5000.

    Techniques: H&E Stain, Immunohistochemistry

    Insoluble immune complexes in sinus macrophages of control lymph nodes from patients with reactive lymphadenitis. In a representative case of control lymph nodes with IICs from patients with reactive lymphadenitis, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, IgA- and IgM-positive SRBs were detected (C and D, respectively), and PAB-reactive SRBs were also detected with a small increase in the number after MT treatment (F). Scale bar: 20 μm.

    Journal: PLoS ONE

    Article Title: Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis

    doi: 10.1371/journal.pone.0192408

    Figure Lengend Snippet: Insoluble immune complexes in sinus macrophages of control lymph nodes from patients with reactive lymphadenitis. In a representative case of control lymph nodes with IICs from patients with reactive lymphadenitis, identical areas of the lymphatic sinus are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, IgA- and IgM-positive SRBs were detected (C and D, respectively), and PAB-reactive SRBs were also detected with a small increase in the number after MT treatment (F). Scale bar: 20 μm.

    Article Snippet: After the initial incubation, the plates were further incubated for 60 min at 37°C with unlabeled mouse anti-PLTA antibody (PAB) diluted 1:500; unlabeled rabbit anti-human C1q and C3c (DAKO 0136 and A062, respectively) diluted 1:500; or biotinylated goat anti-human IgG, IgA, or IgM antibody (Invitrogen, Carlsbad, CA, USA) diluted 1:5000.

    Techniques: H&E Stain, Immunohistochemistry

    P . acnes -derived insoluble immune complexes in sinus macrophages of sarcoid lymph nodes. In a representative case of sarcoid lymph nodes, identical areas of the lesion including a lymphatic sinus and adjacent paracortical area with a sarcoid granuloma are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, the distributions of IgA- and IgM-positive SRBs (C and D) and PAB-reactive SRBs (F) were similar. Note the few PAB-reactive SRBs with weak intensity (indicated by the arrow) in the granuloma after MT treatment (F). Scale bar: 20 μm.

    Journal: PLoS ONE

    Article Title: Propionibacterium acnes-derived insoluble immune complexes in sinus macrophages of lymph nodes affected by sarcoidosis

    doi: 10.1371/journal.pone.0192408

    Figure Lengend Snippet: P . acnes -derived insoluble immune complexes in sinus macrophages of sarcoid lymph nodes. In a representative case of sarcoid lymph nodes, identical areas of the lesion including a lymphatic sinus and adjacent paracortical area with a sarcoid granuloma are shown in semi-serial sections; HE stain (A), IHC with anti-human IgG (B), IgA (C), and IgM (D) antibody, IHC with PAB antibody (E), and IHC with PAB antibody after MT treatment (F). In the sinus macrophages, the distributions of IgA- and IgM-positive SRBs (C and D) and PAB-reactive SRBs (F) were similar. Note the few PAB-reactive SRBs with weak intensity (indicated by the arrow) in the granuloma after MT treatment (F). Scale bar: 20 μm.

    Article Snippet: After the initial incubation, the plates were further incubated for 60 min at 37°C with unlabeled mouse anti-PLTA antibody (PAB) diluted 1:500; unlabeled rabbit anti-human C1q and C3c (DAKO 0136 and A062, respectively) diluted 1:500; or biotinylated goat anti-human IgG, IgA, or IgM antibody (Invitrogen, Carlsbad, CA, USA) diluted 1:5000.

    Techniques: Derivative Assay, H&E Stain, Immunohistochemistry