Journal: Cancer discovery
Article Title: TET2 deficiency causes germinal center hyperplasia, impairs plasma cell differentiation and promotes B-cell lymphomagenesis
Figure Lengend Snippet: Tet2 deletion in hematopoietic cells induces GC hyperplasia. A, Representative flow cytometry plot and quantification of (B220 + CD95 + GL7 + ) GC B-cells from Vav-Cre/ Tet2 +/+ (n=5) and Vav-Cre/ Tet2 −/− (n=5) mice at day 10 after immunization with SRBC. B , Quantification of flow cytometry data corresponding to total B cells (B220 + ), mature B cells (B220 + IgD + IgM + ), transitional B cells (B220 + IgD int IgM + ), follicular B cells (B220 + CD23 + CD21 + ), marginal zone B cells (B220 + CD23 low CD21 + ) and plasmablasts/PC (CD138 + ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. C , Representative histologic sections of formalin-fixed, paraffin-embedded spleens from Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. Sections were stained with H E and antibodies specific for B220, PNA and Ki-67. D , E , Quantification of GC area ( D ) and number of GCs ( E ) in the spleens of Vav-Cre/ Tet2 +/+ and Vav-Cre/ Tet2 −/− mice. two-tailed t test, ***p
Article Snippet: Flow cytometry analysis of spleen cell suspensions was performed using the following fluorescent-labeled anti–mouse antibodies: APC-conjugated anti-B220 (BD Biosciences #553092), anti-CD138 (BD Biosciences #558626) and anti-IgM (eBioscience #17–5790-82); PE-conjugated anti-IgD (BD Biosciences #558597), anti-CD23 (eBioscience #5010271) and anti-CD95 (BD Biosciences #554258); PECy7-conjugated anti-CD21 (BioLegend #123420), anti-CD95 (BD Biosciences #557653), anti-GL7 (BD Biosciences # 561530) and anti-CD86 (BD Biosciences #560582); PEVio770-conjugated anti-B220 (Miltenyi Biotec #130–102-308); FITC-conjugated anti-CXCR4 (BD Biosciences #551967) and anti-IgG1 (ebioscience #11–4011-85); Brilliant Violet 421-conjugated anti-CD138 (BioLegend #142507); PerCP-Cy5.5-conjugated anti-GL7 (BioLegend #144610), anti-CD138 (BioLegend #142510) and anti-IgM (BD Biosciences #550881), APC-Cy7-conjugated anti-CD19 (ebioscience #47–0193-82), AlexaFluor647-conjugated anti-BLIMP1 (BD Biosciences #563643).
Techniques: Flow Cytometry, Mouse Assay, Formalin-fixed Paraffin-Embedded, Staining, Two Tailed Test