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  • 85
    Thermo Fisher immunoglobulin m igm fragmentation kit
    Immunoglobulin M Igm Fragmentation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore immunoglobulin m igm
    Immunoglobulin M Igm, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bethyl immunoglobulin m igm
    The mean (±SD) serum IgG, <t>IgM</t> and IgA concentration of sows in different experimental groups; * – statistical difference compared to control group
    Immunoglobulin M Igm, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Boster Bio immunoglobulin m igm
    Antibody responses and protection in mice immunized with LBNSE-OX40L. ICR mice ( n = 10) were immunized via intramuscular (im) route with 100 μL volume containing 10 6 FFU of LBNSE or LBNSE-OX40L, or an equal volume of DMEM (as mock). Sera from vaccinated mice were collected weekly for nine weeks, and the virus-neutralizing antibodies (VNA) titers ( A ) and GMT ( B ) of sera were determined by using fluorescent antibody virus neutralization (FAVN). ( C ) The optical density (OD) values of total RABV G-specific immunoglobulin G (IgG), IgG1, IgG2a, IgG2b, IgG3, and <t>immunoglobulin</t> M <t>(IgM)</t> from immunized-mice sera were determined by ELISA. At 28 dpi, all the immunized mice ( n = 15) were challenged with 50 LD 50 of CVS-24 via ic route, and then they were carefully monitored daily for 21 days. The survival diagram was constructed according to the number of survivors in each group ( D ). Error bars represented the SE. The following notations were used to indicate significant differences between groups: *, p
    Immunoglobulin M Igm, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Fuller Laboratories immunoglobulin m igm
    Antibody responses and protection in mice immunized with LBNSE-OX40L. ICR mice ( n = 10) were immunized via intramuscular (im) route with 100 μL volume containing 10 6 FFU of LBNSE or LBNSE-OX40L, or an equal volume of DMEM (as mock). Sera from vaccinated mice were collected weekly for nine weeks, and the virus-neutralizing antibodies (VNA) titers ( A ) and GMT ( B ) of sera were determined by using fluorescent antibody virus neutralization (FAVN). ( C ) The optical density (OD) values of total RABV G-specific immunoglobulin G (IgG), IgG1, IgG2a, IgG2b, IgG3, and <t>immunoglobulin</t> M <t>(IgM)</t> from immunized-mice sera were determined by ELISA. At 28 dpi, all the immunized mice ( n = 15) were challenged with 50 LD 50 of CVS-24 via ic route, and then they were carefully monitored daily for 21 days. The survival diagram was constructed according to the number of survivors in each group ( D ). Error bars represented the SE. The following notations were used to indicate significant differences between groups: *, p
    Immunoglobulin M Igm, supplied by Fuller Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Jackson Immuno anti immunoglobulin m igm
    T-oligo treatment does not cause apoptosis in normal primary B or T cells. ( a ) Primary B-2 cells were purified from mouse spleens, cultured and stimulated with 1 µg/ml goat anti-mouse <t>IgM</t> F(ab′) 2 antibody (μ chain specific), 1
    Anti Immunoglobulin M Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore antihuman immunoglobulin m igm
    T-oligo treatment does not cause apoptosis in normal primary B or T cells. ( a ) Primary B-2 cells were purified from mouse spleens, cultured and stimulated with 1 µg/ml goat anti-mouse <t>IgM</t> F(ab′) 2 antibody (μ chain specific), 1
    Antihuman Immunoglobulin M Igm, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Pharmingen immunoglobulin m igm antibodies
    Induction of Ca 2+ mobilization and tyrosine phosphorylation in p110δ-deficient mice. (A) Induction of Ca 2+ was measured by flow cytometry following stimulation of Indo-1-labeled splenic B cells with <t>anti-IgM</t> or of splenic T cells with anti-CD3 followed by anti-IgG. Arrow 1, time point of specific stimulation; arrow 2, time point of ionomycin addition. The results are representative of those from four independent experiments. WT, wild type. (B) Purified splenic B cells from wild-type and p110δ −/− mice were stimulated with anti-IgM (20 μg/ml) for 3 min and analyzed for the induction of tyrosine phosphorylation (pTyr) of Btk and PLCγ2. IP, immunoprecipitation.
    Immunoglobulin M Igm Antibodies, supplied by Pharmingen, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech immunoglobulin m igm biotin
    Unimpaired lymphocyte development in ALX-deficient mice. Wild-type (WT) and ALX-deficient (ALX KO) littermates were analyzed at 8 weeks of age for developmental defects in T- and B-cell development by FACS analysis. (A) CD4 − CD8 − , CD4 + CD8 + , CD4 + , and CD8 + thymocyte populations were similar between wild-type and ALX-deficient mice. (B) Developing B cells in the bone marrow were gated for B220 + AA4.1 + (to exclude recirculating mature B cells) and further subdivided into pro-B-cell (CD43 + <t>IgM</t> − ), pre-B-cell (CD43 − IgM − ), and immature B-cell (CD43 − IgM + ) populations. No differences were observed for any of these populations, demonstrating that B-cell development proceeds normally in ALX-deficient mice. (C) Splenocytes stained with CD4 and CD8 revealed no differences in the proportions of CD4 and CD8 T cells in the periphery. To analyze mature B-cell populations, splenocytes were first gated for B220 + AA4.1 − (to exclude transitional cells) and further subdivided into IgM + CD21/35 + follicular B cells and IgM high CD21/35 high marginal-zone B cells. Again, no differences in the mature B-cell populations in terms of number or proportion were observed for ALX-deficient mice.
    Immunoglobulin M Igm Biotin, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson immunoglobulin m igm
    Increased levels of serum immunoglobulin G1 (IgG1) in Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice. (a) Spontaneous increases in circulating serum IgG1 levels were detected in the sera of 8-week-old Pten +/– SHIP +/– mice, as compared to the other genotypes, and this pattern remained steady and elevated over time. (b) Serum levels of circulating <t>immunoglobulin</t> M <t>(IgM)</t> were not significantly increased over all genotypes at the 8-week point, but steadily increased, becoming significant at later time-points. (c) Levels of circulating immunoglobulin G2b (IgG2b) did not achieve statistical significance compared to all genotypes until the 24-week time-point. Values were representative of sera from at least nine animals per genotype, assessed in duplicate. Error bars represent the standard error of the mean (SEM) of the duplicate readings. * P
    Immunoglobulin M Igm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SouthernBiotech immunoglobulin m igm
    Bortezomib-mediated long-lived plasma cell (LLPC) depletion is transient. Twelve- to 16-wk-old NZB/W F1 mice were treated with one bortezomib cycle consisting of two bortezomib injections within 36 h and analyzed 12 h and 3, 7 and 15 days after the last bortezomib injection. (A) Representative gating strategy for plasma cell analysis by flow cytometry. Lymphocytes were gated according to their position in forward and sideward scatter, and doublets were excluded from the analysis (not shown). Plasma cells were identified as CD138+ and intracellular kappa light chain + cells both in spleen and bone marrow. Within the plasma cells population, plasmablasts and newly generated plasma cells were identified as cells with a higher expression of MHC-II. Conversely, MHC-II low plasma cells were regarded as LLPCs. A representative plot of the expression of MHC-II on the plasma cell population is shown for spleen and bone marrow 15 days after bortezomib treatment. (B) Absolute numbers of all CD138+/intracellular kappa light chain + plasma cells (white bars) and the subgroups of short-lived, MHC high (gray bars) and long-lived, MHC-II low (black bars) plasma cells in spleens (left) and bone marrows (right) 12 h (0.5 days), 3, 7 and 15 days after one cycle of bortezomib measured by flow cytometry. n = 7 mice per time point. (C) Frequencies of the remaining anti-double-stranded DNA (dsDNA) antibody-secreting cells (ASCs) (immunoglobulin G (IgG) and <t>immunoglobulin</t> M <t>(IgM)</t> combined) in spleen (left) and bone marrow (right) calculated by comparison with untreated mice (day 0), as detected by enzyme-linked immunospot (ELISPOT). n = 4 mice per time point. Data are presented as mean and standard error of the mean (SEM). n.s., nonsignificant. P > 0.05, * P ≤0.05, ** P ≤0.01 by two-tailed unpaired t test.
    Immunoglobulin M Igm, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 91/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Abcam immunoglobulin m igm
    Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, <t>IgM,</t> PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.
    Immunoglobulin M Igm, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Focus Diagnostics Inc immunoglobulin m igm
    Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, <t>IgM,</t> PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.
    Immunoglobulin M Igm, supplied by Focus Diagnostics Inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson mouse normal immunoglobulin m igm
    Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, <t>IgM,</t> PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.
    Mouse Normal Immunoglobulin M Igm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies immunoglobulin m igm
    Comparison of results obtained by the indirect ELISAs for the detection of HTNV-specific <t>IgG</t> (a) and <t>IgM</t> (b) antibodies in serum and oral fluid. Horizontal dotted lines, cutoff values for oral fluid assays; vertical dotted lines, cutoff values for serum
    Immunoglobulin M Igm, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Diatec immunoglobulin m igm
    Comparison of results obtained by the indirect ELISAs for the detection of HTNV-specific <t>IgG</t> (a) and <t>IgM</t> (b) antibodies in serum and oral fluid. Horizontal dotted lines, cutoff values for oral fluid assays; vertical dotted lines, cutoff values for serum
    Immunoglobulin M Igm, supplied by Diatec, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Jackson Immuno antihuman immunoglobulin m igm hrp
    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific <t>immunoglobulin</t> M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; <t>IgM,</t> immunoglobulin M; TCID 50 , 50% tissue culture infective dose.
    Antihuman Immunoglobulin M Igm Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Immunology Consultant Laboratory immunoglobulin m igm capture antibody
    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific <t>immunoglobulin</t> M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; <t>IgM,</t> immunoglobulin M; TCID 50 , 50% tissue culture infective dose.
    Immunoglobulin M Igm Capture Antibody, supplied by Immunology Consultant Laboratory, used in various techniques. Bioz Stars score: 88/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    CTK Biotech anti hav immunoglobulin m igm
    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific <t>immunoglobulin</t> M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; <t>IgM,</t> immunoglobulin M; TCID 50 , 50% tissue culture infective dose.
    Anti Hav Immunoglobulin M Igm, supplied by CTK Biotech, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    SouthernBiotech human α immunoglobulin m α igm
    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific <t>immunoglobulin</t> M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; <t>IgM,</t> immunoglobulin M; TCID 50 , 50% tissue culture infective dose.
    Human α Immunoglobulin M α Igm, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beckman Coulter anti gb3 cd77 immunoglobulin m igm
    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific <t>immunoglobulin</t> M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; <t>IgM,</t> immunoglobulin M; TCID 50 , 50% tissue culture infective dose.
    Anti Gb3 Cd77 Immunoglobulin M Igm, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher immunoglobulin m igm
    Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific <t>immunoglobulin</t> M <t>(IgM)</t> and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P
    Immunoglobulin M Igm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Diagast anti d rh1 igm1
    Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific <t>immunoglobulin</t> M <t>(IgM)</t> and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P
    Anti D Rh1 Igm1, supplied by Diagast, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The mean (±SD) serum IgG, IgM and IgA concentration of sows in different experimental groups; * – statistical difference compared to control group

    Journal: Journal of Veterinary Research

    Article Title: Effects of Newly Developed Synbiotic and Commercial Probiotic Products on The Haematological Indices, Serum Cytokines, Acute Phase Proteins Concentration, and Serum Immunoglobulins Amount in Sows and Growing Pigs – A Pilot Study

    doi: 10.2478/jvetres-2018-0046

    Figure Lengend Snippet: The mean (±SD) serum IgG, IgM and IgA concentration of sows in different experimental groups; * – statistical difference compared to control group

    Article Snippet: Measurement of serum immunoglobulins concentration Total serum concentrations of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were determined with a commercial ELISA kit (ELISA Quantitation Kit, Bethyl Laboratories Inc, USA), according to the manufacturer’s recommendations.

    Techniques: Concentration Assay

    The mean (±SD) serum IgG, IgM, and IgA concentration of growing pigs in different experimental groups; * – statistical difference compared to control group

    Journal: Journal of Veterinary Research

    Article Title: Effects of Newly Developed Synbiotic and Commercial Probiotic Products on The Haematological Indices, Serum Cytokines, Acute Phase Proteins Concentration, and Serum Immunoglobulins Amount in Sows and Growing Pigs – A Pilot Study

    doi: 10.2478/jvetres-2018-0046

    Figure Lengend Snippet: The mean (±SD) serum IgG, IgM, and IgA concentration of growing pigs in different experimental groups; * – statistical difference compared to control group

    Article Snippet: Measurement of serum immunoglobulins concentration Total serum concentrations of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were determined with a commercial ELISA kit (ELISA Quantitation Kit, Bethyl Laboratories Inc, USA), according to the manufacturer’s recommendations.

    Techniques: Concentration Assay

    Antibody responses and protection in mice immunized with LBNSE-OX40L. ICR mice ( n = 10) were immunized via intramuscular (im) route with 100 μL volume containing 10 6 FFU of LBNSE or LBNSE-OX40L, or an equal volume of DMEM (as mock). Sera from vaccinated mice were collected weekly for nine weeks, and the virus-neutralizing antibodies (VNA) titers ( A ) and GMT ( B ) of sera were determined by using fluorescent antibody virus neutralization (FAVN). ( C ) The optical density (OD) values of total RABV G-specific immunoglobulin G (IgG), IgG1, IgG2a, IgG2b, IgG3, and immunoglobulin M (IgM) from immunized-mice sera were determined by ELISA. At 28 dpi, all the immunized mice ( n = 15) were challenged with 50 LD 50 of CVS-24 via ic route, and then they were carefully monitored daily for 21 days. The survival diagram was constructed according to the number of survivors in each group ( D ). Error bars represented the SE. The following notations were used to indicate significant differences between groups: *, p

    Journal: Vaccines

    Article Title: Recombinant Rabies Virus Overexpressing OX40-Ligand Enhances Humoral Immune Responses by Increasing T Follicular Helper Cells and Germinal Center B Cells

    doi: 10.3390/vaccines8010144

    Figure Lengend Snippet: Antibody responses and protection in mice immunized with LBNSE-OX40L. ICR mice ( n = 10) were immunized via intramuscular (im) route with 100 μL volume containing 10 6 FFU of LBNSE or LBNSE-OX40L, or an equal volume of DMEM (as mock). Sera from vaccinated mice were collected weekly for nine weeks, and the virus-neutralizing antibodies (VNA) titers ( A ) and GMT ( B ) of sera were determined by using fluorescent antibody virus neutralization (FAVN). ( C ) The optical density (OD) values of total RABV G-specific immunoglobulin G (IgG), IgG1, IgG2a, IgG2b, IgG3, and immunoglobulin M (IgM) from immunized-mice sera were determined by ELISA. At 28 dpi, all the immunized mice ( n = 15) were challenged with 50 LD 50 of CVS-24 via ic route, and then they were carefully monitored daily for 21 days. The survival diagram was constructed according to the number of survivors in each group ( D ). Error bars represented the SE. The following notations were used to indicate significant differences between groups: *, p

    Article Snippet: These antibodies were used for detection of RABV-specific antibody isotypes, including horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG), IgG1, IgG2a, IgG2b, IgG3, and immunoglobulin M (IgM) (Boster, Wuhan, China).

    Techniques: Mouse Assay, Neutralization, Enzyme-linked Immunosorbent Assay, Construct

    T-oligo treatment does not cause apoptosis in normal primary B or T cells. ( a ) Primary B-2 cells were purified from mouse spleens, cultured and stimulated with 1 µg/ml goat anti-mouse IgM F(ab′) 2 antibody (μ chain specific), 1

    Journal: International journal of cancer. Journal international du cancer

    Article Title: Telomere homolog oligonucleotides induce apoptosis in malignant but not in normal lymphoid cells: Mechanism and therapeutic potential

    doi: 10.1002/ijc.23946

    Figure Lengend Snippet: T-oligo treatment does not cause apoptosis in normal primary B or T cells. ( a ) Primary B-2 cells were purified from mouse spleens, cultured and stimulated with 1 µg/ml goat anti-mouse IgM F(ab′) 2 antibody (μ chain specific), 1

    Article Snippet: Primary B cells were isolated from normal mouse spleens by anti-CD43 negative selection with magnetic beads (21; Miltenyi Biotec, Auburn, CA) and stimulated with anti-immunoglobulin M (IgM) (Jackson ImmunoResearch, West Grove, PA) and anti-CD40 antibodies (BD Pharmingen, San Diego, CA) and interleukin-4 (eBioscience, San Diego, CA).

    Techniques: Purification, Cell Culture

    Induction of Ca 2+ mobilization and tyrosine phosphorylation in p110δ-deficient mice. (A) Induction of Ca 2+ was measured by flow cytometry following stimulation of Indo-1-labeled splenic B cells with anti-IgM or of splenic T cells with anti-CD3 followed by anti-IgG. Arrow 1, time point of specific stimulation; arrow 2, time point of ionomycin addition. The results are representative of those from four independent experiments. WT, wild type. (B) Purified splenic B cells from wild-type and p110δ −/− mice were stimulated with anti-IgM (20 μg/ml) for 3 min and analyzed for the induction of tyrosine phosphorylation (pTyr) of Btk and PLCγ2. IP, immunoprecipitation.

    Journal: Molecular and Cellular Biology

    Article Title: Essential, Nonredundant Role for the Phosphoinositide 3-Kinase p110? in Signaling by the B-Cell Receptor Complex

    doi: 10.1128/MCB.22.24.8580-8591.2002

    Figure Lengend Snippet: Induction of Ca 2+ mobilization and tyrosine phosphorylation in p110δ-deficient mice. (A) Induction of Ca 2+ was measured by flow cytometry following stimulation of Indo-1-labeled splenic B cells with anti-IgM or of splenic T cells with anti-CD3 followed by anti-IgG. Arrow 1, time point of specific stimulation; arrow 2, time point of ionomycin addition. The results are representative of those from four independent experiments. WT, wild type. (B) Purified splenic B cells from wild-type and p110δ −/− mice were stimulated with anti-IgM (20 μg/ml) for 3 min and analyzed for the induction of tyrosine phosphorylation (pTyr) of Btk and PLCγ2. IP, immunoprecipitation.

    Article Snippet: Monoclonal antibodies (MAbs) conjugated to either fluorescein isothiocyanate (FITC), phycoerythrin (PE), or CyChrome and specific to murine B220/CD45R, immunoglobulin M (IgM), IgD, CD5, CD43, Thy1.2, interleukin-2 (IL-2) receptor (CD25), CD44, CD4, CD8, Mac-1 (CD11b), and Gr-1 (all from PharMingen) were used to label 106 cells for 30 min on ice.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Labeling, Purification, Immunoprecipitation

    Unimpaired lymphocyte development in ALX-deficient mice. Wild-type (WT) and ALX-deficient (ALX KO) littermates were analyzed at 8 weeks of age for developmental defects in T- and B-cell development by FACS analysis. (A) CD4 − CD8 − , CD4 + CD8 + , CD4 + , and CD8 + thymocyte populations were similar between wild-type and ALX-deficient mice. (B) Developing B cells in the bone marrow were gated for B220 + AA4.1 + (to exclude recirculating mature B cells) and further subdivided into pro-B-cell (CD43 + IgM − ), pre-B-cell (CD43 − IgM − ), and immature B-cell (CD43 − IgM + ) populations. No differences were observed for any of these populations, demonstrating that B-cell development proceeds normally in ALX-deficient mice. (C) Splenocytes stained with CD4 and CD8 revealed no differences in the proportions of CD4 and CD8 T cells in the periphery. To analyze mature B-cell populations, splenocytes were first gated for B220 + AA4.1 − (to exclude transitional cells) and further subdivided into IgM + CD21/35 + follicular B cells and IgM high CD21/35 high marginal-zone B cells. Again, no differences in the mature B-cell populations in terms of number or proportion were observed for ALX-deficient mice.

    Journal: Molecular and Cellular Biology

    Article Title: Negative Regulation of Interleukin-2 and p38 Mitogen-Activated Protein Kinase during T-Cell Activation by the Adaptor ALX

    doi: 10.1128/MCB.02067-05

    Figure Lengend Snippet: Unimpaired lymphocyte development in ALX-deficient mice. Wild-type (WT) and ALX-deficient (ALX KO) littermates were analyzed at 8 weeks of age for developmental defects in T- and B-cell development by FACS analysis. (A) CD4 − CD8 − , CD4 + CD8 + , CD4 + , and CD8 + thymocyte populations were similar between wild-type and ALX-deficient mice. (B) Developing B cells in the bone marrow were gated for B220 + AA4.1 + (to exclude recirculating mature B cells) and further subdivided into pro-B-cell (CD43 + IgM − ), pre-B-cell (CD43 − IgM − ), and immature B-cell (CD43 − IgM + ) populations. No differences were observed for any of these populations, demonstrating that B-cell development proceeds normally in ALX-deficient mice. (C) Splenocytes stained with CD4 and CD8 revealed no differences in the proportions of CD4 and CD8 T cells in the periphery. To analyze mature B-cell populations, splenocytes were first gated for B220 + AA4.1 − (to exclude transitional cells) and further subdivided into IgM + CD21/35 + follicular B cells and IgM high CD21/35 high marginal-zone B cells. Again, no differences in the mature B-cell populations in terms of number or proportion were observed for ALX-deficient mice.

    Article Snippet: The following fluorescence-activated cell sorter (FACS) reagents were used: B220-allophycocyanin (APC)-Cy7 (eBiosciences), AA4.1-APC (eBiosciences), CD43-fluorescein isothiocyanate (FITC) (Pharmingen), immunoglobulin M (IgM)-biotin (Southern Biotechnology), streptavidin-peridinin chlorophyll a protein-Cy5.5 (Pharmingen), CD4-phycoerythrin (PE) (Caltag), CD8-APC (Caltag), CD44-FITC (Pharmingen), CD62L-PE (Pharmingen), CD4-biotin (Pharmingen), CD25-APC (Pharmingen), and CD69-FITC (Pharmingen).

    Techniques: Mouse Assay, FACS, Staining

    Intact T-cell-dependent B-cell responses to antigen in vivo. (A) Serum immunoglobulin levels from wild-type and ALX-deficient mice were analyzed by ELISA. The data represent averages for six wild-type and nine ALX-deficient mice between 12 and 15 weeks of age. The error bars reflect the standard deviations within each group. (B) Five wild-type and four ALX-deficient mice between 9 and 10 weeks of age were immunized with 100 μg alum-precipitated NP-KLH/mouse on day 0, boosted on day 47, and sacrificed on day 54. Sera were collected at the time points shown and were assayed for the concentration of high affinity anti-NP IgG compared to that of the NP-specific IgG1 monoclonal antibody B1-8 used as a standard. The points represent the average concentrations for all wild-type mice or ALX-deficient mice. Error bars reflect the standard deviations within each genotype. (C) Purified B cells from wild-type and ALX-deficient mice were labeled with CSFE and either were left unstimulated or were stimulated with a combination of anti-IgM, anti-CD40, BLyS, CpG, and/or LPS as shown in the figure and as described in Materials and Methods. After 3 days, the cells were analyzed by FACS. TOPRO-3 (100 nM) was added to exclude dead cells from further analysis. A fixed number of 6-μm beads were added to each sample to permit calculation of the absolute number of live cells within each sample. The y axis represents absolute numbers of live B cells. For each stimulation condition, the gray-filled histograms represent wild-type B cells and the solid black lines represent ALX-deficient B cells. The results shown are representative of three separate experiments. WT, wild type; ALX KO, ALX deficient.

    Journal: Molecular and Cellular Biology

    Article Title: Negative Regulation of Interleukin-2 and p38 Mitogen-Activated Protein Kinase during T-Cell Activation by the Adaptor ALX

    doi: 10.1128/MCB.02067-05

    Figure Lengend Snippet: Intact T-cell-dependent B-cell responses to antigen in vivo. (A) Serum immunoglobulin levels from wild-type and ALX-deficient mice were analyzed by ELISA. The data represent averages for six wild-type and nine ALX-deficient mice between 12 and 15 weeks of age. The error bars reflect the standard deviations within each group. (B) Five wild-type and four ALX-deficient mice between 9 and 10 weeks of age were immunized with 100 μg alum-precipitated NP-KLH/mouse on day 0, boosted on day 47, and sacrificed on day 54. Sera were collected at the time points shown and were assayed for the concentration of high affinity anti-NP IgG compared to that of the NP-specific IgG1 monoclonal antibody B1-8 used as a standard. The points represent the average concentrations for all wild-type mice or ALX-deficient mice. Error bars reflect the standard deviations within each genotype. (C) Purified B cells from wild-type and ALX-deficient mice were labeled with CSFE and either were left unstimulated or were stimulated with a combination of anti-IgM, anti-CD40, BLyS, CpG, and/or LPS as shown in the figure and as described in Materials and Methods. After 3 days, the cells were analyzed by FACS. TOPRO-3 (100 nM) was added to exclude dead cells from further analysis. A fixed number of 6-μm beads were added to each sample to permit calculation of the absolute number of live cells within each sample. The y axis represents absolute numbers of live B cells. For each stimulation condition, the gray-filled histograms represent wild-type B cells and the solid black lines represent ALX-deficient B cells. The results shown are representative of three separate experiments. WT, wild type; ALX KO, ALX deficient.

    Article Snippet: The following fluorescence-activated cell sorter (FACS) reagents were used: B220-allophycocyanin (APC)-Cy7 (eBiosciences), AA4.1-APC (eBiosciences), CD43-fluorescein isothiocyanate (FITC) (Pharmingen), immunoglobulin M (IgM)-biotin (Southern Biotechnology), streptavidin-peridinin chlorophyll a protein-Cy5.5 (Pharmingen), CD4-phycoerythrin (PE) (Caltag), CD8-APC (Caltag), CD44-FITC (Pharmingen), CD62L-PE (Pharmingen), CD4-biotin (Pharmingen), CD25-APC (Pharmingen), and CD69-FITC (Pharmingen).

    Techniques: In Vivo, Mouse Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Purification, Labeling, FACS

    Increased levels of serum immunoglobulin G1 (IgG1) in Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice. (a) Spontaneous increases in circulating serum IgG1 levels were detected in the sera of 8-week-old Pten +/– SHIP +/– mice, as compared to the other genotypes, and this pattern remained steady and elevated over time. (b) Serum levels of circulating immunoglobulin M (IgM) were not significantly increased over all genotypes at the 8-week point, but steadily increased, becoming significant at later time-points. (c) Levels of circulating immunoglobulin G2b (IgG2b) did not achieve statistical significance compared to all genotypes until the 24-week time-point. Values were representative of sera from at least nine animals per genotype, assessed in duplicate. Error bars represent the standard error of the mean (SEM) of the duplicate readings. * P

    Journal: Immunology

    Article Title: Compound heterozygosity for Pten and SHIP augments T-dependent humoral immune responses and cytokine production by CD4+ T cells

    doi: 10.1111/j.1365-2567.2004.01901.x

    Figure Lengend Snippet: Increased levels of serum immunoglobulin G1 (IgG1) in Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice. (a) Spontaneous increases in circulating serum IgG1 levels were detected in the sera of 8-week-old Pten +/– SHIP +/– mice, as compared to the other genotypes, and this pattern remained steady and elevated over time. (b) Serum levels of circulating immunoglobulin M (IgM) were not significantly increased over all genotypes at the 8-week point, but steadily increased, becoming significant at later time-points. (c) Levels of circulating immunoglobulin G2b (IgG2b) did not achieve statistical significance compared to all genotypes until the 24-week time-point. Values were representative of sera from at least nine animals per genotype, assessed in duplicate. Error bars represent the standard error of the mean (SEM) of the duplicate readings. * P

    Article Snippet: ELISA quantification of serum immunoglobulin levels was performed, as previously described, using capture and biotinylated detection antibodies for immunoglobulin G1 (IgG1), immunoglobulin G2b (IgG2b) and immunoglobulin M (IgM) (BD Pharmingen).

    Techniques: Mouse Assay

    Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice show an increased humoral response to the T-dependent antigen, nitrophenylacetyl (NP)-keyhole limpet haemocyanin (NP-KLH). Serum antibodies against NP-bovine serum albumin (NP-BSA) were assessed from six or seven mice of each genotype that had been challenged with NP-Ficoll or NP-KLH. Anti-NP titres [immunoglobulin M (IgM) and pan immunoglobulin G (IgG)] were determined by enzyme-linked immunosorbent assay (ELISA) on serially diluted serum samples. Bars indicate the average titre, and triangles represent individual mice. No differences were observed in either the IgM or IgG response to NP-Ficoll (a, b). However, statistically significant differences in IgM and IgG responses to NP-KLH were detected (c, d). * P

    Journal: Immunology

    Article Title: Compound heterozygosity for Pten and SHIP augments T-dependent humoral immune responses and cytokine production by CD4+ T cells

    doi: 10.1111/j.1365-2567.2004.01901.x

    Figure Lengend Snippet: Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice show an increased humoral response to the T-dependent antigen, nitrophenylacetyl (NP)-keyhole limpet haemocyanin (NP-KLH). Serum antibodies against NP-bovine serum albumin (NP-BSA) were assessed from six or seven mice of each genotype that had been challenged with NP-Ficoll or NP-KLH. Anti-NP titres [immunoglobulin M (IgM) and pan immunoglobulin G (IgG)] were determined by enzyme-linked immunosorbent assay (ELISA) on serially diluted serum samples. Bars indicate the average titre, and triangles represent individual mice. No differences were observed in either the IgM or IgG response to NP-Ficoll (a, b). However, statistically significant differences in IgM and IgG responses to NP-KLH were detected (c, d). * P

    Article Snippet: ELISA quantification of serum immunoglobulin levels was performed, as previously described, using capture and biotinylated detection antibodies for immunoglobulin G1 (IgG1), immunoglobulin G2b (IgG2b) and immunoglobulin M (IgM) (BD Pharmingen).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Bortezomib-mediated long-lived plasma cell (LLPC) depletion is transient. Twelve- to 16-wk-old NZB/W F1 mice were treated with one bortezomib cycle consisting of two bortezomib injections within 36 h and analyzed 12 h and 3, 7 and 15 days after the last bortezomib injection. (A) Representative gating strategy for plasma cell analysis by flow cytometry. Lymphocytes were gated according to their position in forward and sideward scatter, and doublets were excluded from the analysis (not shown). Plasma cells were identified as CD138+ and intracellular kappa light chain + cells both in spleen and bone marrow. Within the plasma cells population, plasmablasts and newly generated plasma cells were identified as cells with a higher expression of MHC-II. Conversely, MHC-II low plasma cells were regarded as LLPCs. A representative plot of the expression of MHC-II on the plasma cell population is shown for spleen and bone marrow 15 days after bortezomib treatment. (B) Absolute numbers of all CD138+/intracellular kappa light chain + plasma cells (white bars) and the subgroups of short-lived, MHC high (gray bars) and long-lived, MHC-II low (black bars) plasma cells in spleens (left) and bone marrows (right) 12 h (0.5 days), 3, 7 and 15 days after one cycle of bortezomib measured by flow cytometry. n = 7 mice per time point. (C) Frequencies of the remaining anti-double-stranded DNA (dsDNA) antibody-secreting cells (ASCs) (immunoglobulin G (IgG) and immunoglobulin M (IgM) combined) in spleen (left) and bone marrow (right) calculated by comparison with untreated mice (day 0), as detected by enzyme-linked immunospot (ELISPOT). n = 4 mice per time point. Data are presented as mean and standard error of the mean (SEM). n.s., nonsignificant. P > 0.05, * P ≤0.05, ** P ≤0.01 by two-tailed unpaired t test.

    Journal: Arthritis Research & Therapy

    Article Title: Long-lived plasma cells are early and constantly generated in New Zealand Black/New Zealand White F1 mice and their therapeutic depletion requires a combined targeting of autoreactive plasma cells and their precursors

    doi: 10.1186/s13075-015-0551-3

    Figure Lengend Snippet: Bortezomib-mediated long-lived plasma cell (LLPC) depletion is transient. Twelve- to 16-wk-old NZB/W F1 mice were treated with one bortezomib cycle consisting of two bortezomib injections within 36 h and analyzed 12 h and 3, 7 and 15 days after the last bortezomib injection. (A) Representative gating strategy for plasma cell analysis by flow cytometry. Lymphocytes were gated according to their position in forward and sideward scatter, and doublets were excluded from the analysis (not shown). Plasma cells were identified as CD138+ and intracellular kappa light chain + cells both in spleen and bone marrow. Within the plasma cells population, plasmablasts and newly generated plasma cells were identified as cells with a higher expression of MHC-II. Conversely, MHC-II low plasma cells were regarded as LLPCs. A representative plot of the expression of MHC-II on the plasma cell population is shown for spleen and bone marrow 15 days after bortezomib treatment. (B) Absolute numbers of all CD138+/intracellular kappa light chain + plasma cells (white bars) and the subgroups of short-lived, MHC high (gray bars) and long-lived, MHC-II low (black bars) plasma cells in spleens (left) and bone marrows (right) 12 h (0.5 days), 3, 7 and 15 days after one cycle of bortezomib measured by flow cytometry. n = 7 mice per time point. (C) Frequencies of the remaining anti-double-stranded DNA (dsDNA) antibody-secreting cells (ASCs) (immunoglobulin G (IgG) and immunoglobulin M (IgM) combined) in spleen (left) and bone marrow (right) calculated by comparison with untreated mice (day 0), as detected by enzyme-linked immunospot (ELISPOT). n = 4 mice per time point. Data are presented as mean and standard error of the mean (SEM). n.s., nonsignificant. P > 0.05, * P ≤0.05, ** P ≤0.01 by two-tailed unpaired t test.

    Article Snippet: After extensive washing, plates were incubated with biotin-labeled goat anti-mouse immunoglobulin G (IgG) and immunoglobulin M (IgM) (1 μg/ml, Southern Biotech, Birmingham, AL, USA) for 1 h and followed by ExtrAvidin™-alkaline phosphatase (Sigma-Aldrich) for 20 min.

    Techniques: Mouse Assay, Injection, Flow Cytometry, Cytometry, Generated, Expressing, Enzyme-linked Immunospot, Two Tailed Test

    Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, IgM, PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.

    Journal: PLoS ONE

    Article Title: Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis

    doi: 10.1371/journal.pone.0118527

    Figure Lengend Snippet: Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, IgM, PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) ELISA tests were used to quantify the protein concentration of differentially expressed proteins CPN, CP, complement component 4a (C4a), fibrinogen-alpha (FGA), PON1 (USCN Life Science Inc., Wuhan, China), and immunoglobulin M (IgM) (Abcam, Cambridge, UK) in plasma samples from the control (24 cases) and tumor (22 cases) groups.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    Comparison of results obtained by the indirect ELISAs for the detection of HTNV-specific IgG (a) and IgM (b) antibodies in serum and oral fluid. Horizontal dotted lines, cutoff values for oral fluid assays; vertical dotted lines, cutoff values for serum

    Journal:

    Article Title: Use of Saccharomyces cerevisiae-Expressed Recombinant Nucleocapsid Protein To Detect Hantaan Virus-Specific Immunoglobulin G (IgG) and IgM in Oral Fluid ▿

    doi: 10.1128/CVI.00188-07

    Figure Lengend Snippet: Comparison of results obtained by the indirect ELISAs for the detection of HTNV-specific IgG (a) and IgM (b) antibodies in serum and oral fluid. Horizontal dotted lines, cutoff values for oral fluid assays; vertical dotted lines, cutoff values for serum

    Article Snippet: Peroxidase-conjugated F(ab′)2 fragments of rabbit anti-human IgG or IgM (Dako, Denmark) diluted 1:12,000 in PBS containing 1% BSA and 0.2% Tween 20 were added to the wells, and the plates were incubated for 1 h at 37°C.

    Techniques:

    Comparison of HTNV-specific IgG and IgM measurements in oral fluid and the corresponding serum samples by indirect ELISA.

    Journal:

    Article Title: Use of Saccharomyces cerevisiae-Expressed Recombinant Nucleocapsid Protein To Detect Hantaan Virus-Specific Immunoglobulin G (IgG) and IgM in Oral Fluid ▿

    doi: 10.1128/CVI.00188-07

    Figure Lengend Snippet: Comparison of HTNV-specific IgG and IgM measurements in oral fluid and the corresponding serum samples by indirect ELISA.

    Article Snippet: Peroxidase-conjugated F(ab′)2 fragments of rabbit anti-human IgG or IgM (Dako, Denmark) diluted 1:12,000 in PBS containing 1% BSA and 0.2% Tween 20 were added to the wells, and the plates were incubated for 1 h at 37°C.

    Techniques: Indirect ELISA

    Sodium dodecyl sulfate-polyacrylamide gels (A) and Western immunoblots (B to D) of different hantavirus N proteins with HTNV-positive human sera. (B) Detection of IgG antibodies; (C and D) detection of IgM antibodies. The lanes contain recombinant yeast-expressed

    Journal:

    Article Title: Use of Saccharomyces cerevisiae-Expressed Recombinant Nucleocapsid Protein To Detect Hantaan Virus-Specific Immunoglobulin G (IgG) and IgM in Oral Fluid ▿

    doi: 10.1128/CVI.00188-07

    Figure Lengend Snippet: Sodium dodecyl sulfate-polyacrylamide gels (A) and Western immunoblots (B to D) of different hantavirus N proteins with HTNV-positive human sera. (B) Detection of IgG antibodies; (C and D) detection of IgM antibodies. The lanes contain recombinant yeast-expressed

    Article Snippet: Peroxidase-conjugated F(ab′)2 fragments of rabbit anti-human IgG or IgM (Dako, Denmark) diluted 1:12,000 in PBS containing 1% BSA and 0.2% Tween 20 were added to the wells, and the plates were incubated for 1 h at 37°C.

    Techniques: Western Blot, Recombinant

    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific immunoglobulin M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; IgM, immunoglobulin M; TCID 50 , 50% tissue culture infective dose.

    Journal: The Journal of Infectious Diseases

    Article Title: A Case of Human Lassa Virus Infection With Robust Acute T-Cell Activation and Long-Term Virus-Specific T-Cell Responses

    doi: 10.1093/infdis/jix201

    Figure Lengend Snippet: Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific immunoglobulin M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; IgM, immunoglobulin M; TCID 50 , 50% tissue culture infective dose.

    Article Snippet: After 3 washes in PBST, plates were incubated for 1 hour at 37°C with antihuman IgG HRP (horseradish peroxidase) or antihuman immunoglobulin M (IgM) HRP (Jackson ImmunoResearch Inc) diluted 1:5000 in blocking solution.

    Techniques: Enzyme-linked Immunosorbent Assay, Viral-load Assay, Flow Cytometry

    Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P

    Journal: The Journal of Infectious Diseases

    Article Title: Early Appearance of Neutralizing Immunoglobulin G3 Antibodies Is Associated With Chikungunya Virus Clearance and Long-term Clinical Protection

    doi: 10.1093/infdis/jis033

    Figure Lengend Snippet: Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P

    Article Snippet: Horseradish peroxidase–conjugated mouse antihuman immunoglobulin G (IgG), immunoglobulin G1 (IgG1), immunoglobulin G2 (IgG2), IgG3, immunoglobulin G4 (IgG4), and immunoglobulin M (IgM) (Molecular Probes) were used to detect human antibodies bound to virus-coated wells.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Purification, Staining, MANN-WHITNEY