igm Search Results


goat anti rabbit hrp  (Bio-Rad)
95
Bio-Rad goat anti rabbit hrp
Goat Anti Rabbit Hrp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alp conjugated rabbit anti mouse igm  (Rockland Immunochemicals)
94
Rockland Immunochemicals alp conjugated rabbit anti mouse igm
Alp Conjugated Rabbit Anti Mouse Igm, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti monkey igm  (Rockland Immunochemicals)
93
Rockland Immunochemicals goat anti monkey igm
Goat Anti Monkey Igm, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dpp hiv 1 2 igm  (HyTest)
94
HyTest dpp hiv 1 2 igm
Antibody detection in <t>HIV-1</t> seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the <t>DPP</t> HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.
Dpp Hiv 1 2 Igm, supplied by HyTest, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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igm  (Rockland Immunochemicals)
90
Rockland Immunochemicals igm
Antibody detection in <t>HIV-1</t> seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the <t>DPP</t> HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.
Igm, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
igm - by Bioz Stars, 2026-06
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pentameric igm  (Rockland Immunochemicals)
90
Rockland Immunochemicals pentameric igm
Antibody detection in <t>HIV-1</t> seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the <t>DPP</t> HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.
Pentameric Igm, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentameric igm/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
pentameric igm - by Bioz Stars, 2026-06
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goat polyclonal antiegfp  (Rockland Immunochemicals)
93
Rockland Immunochemicals goat polyclonal antiegfp
Antibody detection in <t>HIV-1</t> seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the <t>DPP</t> HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.
Goat Polyclonal Antiegfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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goat anti mouse secondary polyclonal antibodies conjugated  (R&D Systems)
93
R&D Systems goat anti mouse secondary polyclonal antibodies conjugated
Antibody detection in <t>HIV-1</t> seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the <t>DPP</t> HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.
Goat Anti Mouse Secondary Polyclonal Antibodies Conjugated, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse secondary polyclonal antibodies conjugated/product/R&D Systems
Average 93 stars, based on 1 article reviews
goat anti mouse secondary polyclonal antibodies conjugated - by Bioz Stars, 2026-06
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m chain specific jackson immunoresearch  (Jackson Immuno)
94
Jackson Immuno m chain specific jackson immunoresearch
Antibody detection in <t>HIV-1</t> seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the <t>DPP</t> HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.
M Chain Specific Jackson Immunoresearch, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m chain specific jackson immunoresearch/product/Jackson Immuno
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m chain specific jackson immunoresearch - by Bioz Stars, 2026-06
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cd23  (Jackson Immuno)
93
Jackson Immuno cd23
Cnot3 -deficient mice show impaired early B-cell differentiation that can be alleviated in part by the expression of Mzb1 . ( A ) Flow cytometric analysis of bone marrow B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect B220 + CD43 + pro-B, B220 int CD43 − late pre-B, and immature B cells as well as B220 hi CD43 − recirculating B cells in the living lymphocyte gate. ( B ) Further gating of pro-B cells to detect pre-pro-B (HSA − BP1 − ), early pro-B (HSA + BP1 − ) and late pro-B (HSA + BP1 + ) cells. ( C ) Statistical analysis of the total numbers of pro-B (pink) and pre-B (red) cells in Cnot3 +/+ RERT Cre (wild-type) and Cnot3 fl/fl RERT Cre (knockout) mice. Long horizontal bars indicate the mean, and short horizontal bars represent the SD of the mean. Statistical significance between wild type and knockout is assessed by an unpaired two-tail Student's t -test. ( D ) PCR analysis of genomic DNA from Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect the deletion status of Cnot3 . Cnot6 served as a loading control. ( E ) Flow cytometric analysis of splenic B cells to detect CD21 int <t>CD23</t> + follicular B cells and CD21 hi CD23 − marginal zone B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice. ( F ) Flow cytometric analysis of bone marrow cells in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice to detect the B-cell compartment as described in A . ( G ) Flow cytometric analysis of the effects of a Mzb1 transgene expression ( Mzb1 tg ) on early B-cell differentiation in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice. ( H , I ) Gating of bone marrow B220 + CD43 + pro-B cells to detect HSA + BP1 − early pro-B and HSA + BP1 + late pro-B stages. ( J , K ) Statistical analysis of the total numbers of pre-B (red), recirculating B (blue), early pro-B (orange), and late pro-B (green) cells in Cnot3 +/+ mb1 Cre , Cnot3 fl/fl mb1 Cre , Cnot3 +l+ mb1 Cre Mzb1 tg , and Cnot3 fl/fl mb1 Cre Mzb1 tg mice. Numbers in the FACS profiles indicate the percentage of cells within the gated population. The data are representative of four or more independent experiments.
Cd23, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd23/product/Jackson Immuno
Average 93 stars, based on 1 article reviews
cd23 - by Bioz Stars, 2026-06
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fluorescein fitc affinipuretm  (Jackson Immuno)
93
Jackson Immuno fluorescein fitc affinipuretm
Cnot3 -deficient mice show impaired early B-cell differentiation that can be alleviated in part by the expression of Mzb1 . ( A ) Flow cytometric analysis of bone marrow B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect B220 + CD43 + pro-B, B220 int CD43 − late pre-B, and immature B cells as well as B220 hi CD43 − recirculating B cells in the living lymphocyte gate. ( B ) Further gating of pro-B cells to detect pre-pro-B (HSA − BP1 − ), early pro-B (HSA + BP1 − ) and late pro-B (HSA + BP1 + ) cells. ( C ) Statistical analysis of the total numbers of pro-B (pink) and pre-B (red) cells in Cnot3 +/+ RERT Cre (wild-type) and Cnot3 fl/fl RERT Cre (knockout) mice. Long horizontal bars indicate the mean, and short horizontal bars represent the SD of the mean. Statistical significance between wild type and knockout is assessed by an unpaired two-tail Student's t -test. ( D ) PCR analysis of genomic DNA from Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect the deletion status of Cnot3 . Cnot6 served as a loading control. ( E ) Flow cytometric analysis of splenic B cells to detect CD21 int <t>CD23</t> + follicular B cells and CD21 hi CD23 − marginal zone B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice. ( F ) Flow cytometric analysis of bone marrow cells in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice to detect the B-cell compartment as described in A . ( G ) Flow cytometric analysis of the effects of a Mzb1 transgene expression ( Mzb1 tg ) on early B-cell differentiation in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice. ( H , I ) Gating of bone marrow B220 + CD43 + pro-B cells to detect HSA + BP1 − early pro-B and HSA + BP1 + late pro-B stages. ( J , K ) Statistical analysis of the total numbers of pre-B (red), recirculating B (blue), early pro-B (orange), and late pro-B (green) cells in Cnot3 +/+ mb1 Cre , Cnot3 fl/fl mb1 Cre , Cnot3 +l+ mb1 Cre Mzb1 tg , and Cnot3 fl/fl mb1 Cre Mzb1 tg mice. Numbers in the FACS profiles indicate the percentage of cells within the gated population. The data are representative of four or more independent experiments.
Fluorescein Fitc Affinipuretm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein fitc affinipuretm/product/Jackson Immuno
Average 93 stars, based on 1 article reviews
fluorescein fitc affinipuretm - by Bioz Stars, 2026-06
93/100 stars
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goat anti mouse igm  (Jackson Immuno)
93
Jackson Immuno goat anti mouse igm
Cnot3 -deficient mice show impaired early B-cell differentiation that can be alleviated in part by the expression of Mzb1 . ( A ) Flow cytometric analysis of bone marrow B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect B220 + CD43 + pro-B, B220 int CD43 − late pre-B, and immature B cells as well as B220 hi CD43 − recirculating B cells in the living lymphocyte gate. ( B ) Further gating of pro-B cells to detect pre-pro-B (HSA − BP1 − ), early pro-B (HSA + BP1 − ) and late pro-B (HSA + BP1 + ) cells. ( C ) Statistical analysis of the total numbers of pro-B (pink) and pre-B (red) cells in Cnot3 +/+ RERT Cre (wild-type) and Cnot3 fl/fl RERT Cre (knockout) mice. Long horizontal bars indicate the mean, and short horizontal bars represent the SD of the mean. Statistical significance between wild type and knockout is assessed by an unpaired two-tail Student's t -test. ( D ) PCR analysis of genomic DNA from Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect the deletion status of Cnot3 . Cnot6 served as a loading control. ( E ) Flow cytometric analysis of splenic B cells to detect CD21 int <t>CD23</t> + follicular B cells and CD21 hi CD23 − marginal zone B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice. ( F ) Flow cytometric analysis of bone marrow cells in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice to detect the B-cell compartment as described in A . ( G ) Flow cytometric analysis of the effects of a Mzb1 transgene expression ( Mzb1 tg ) on early B-cell differentiation in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice. ( H , I ) Gating of bone marrow B220 + CD43 + pro-B cells to detect HSA + BP1 − early pro-B and HSA + BP1 + late pro-B stages. ( J , K ) Statistical analysis of the total numbers of pre-B (red), recirculating B (blue), early pro-B (orange), and late pro-B (green) cells in Cnot3 +/+ mb1 Cre , Cnot3 fl/fl mb1 Cre , Cnot3 +l+ mb1 Cre Mzb1 tg , and Cnot3 fl/fl mb1 Cre Mzb1 tg mice. Numbers in the FACS profiles indicate the percentage of cells within the gated population. The data are representative of four or more independent experiments.
Goat Anti Mouse Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
goat anti mouse igm - by Bioz Stars, 2026-06
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Image Search Results


Antibody detection in HIV-1 seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the DPP HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.

Journal: Microbiology Spectrum

Article Title: Rapid Point-of-Care Tests Using Staphylococcal Protein A Can Detect Early IgM Responses in HIV-1 and Treponema pallidum Infections

doi: 10.1128/spectrum.03309-22

Figure Lengend Snippet: Antibody detection in HIV-1 seroconversion panel PRB-927 and effect of sample pretreatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the DPP HIV 1/2 (red lines) and DPP HIV 1/2 IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 03 and 04 collected during initial IgM seroconversion (days 33 and 35 since 1st bleed), in which antibody detection by each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples tested by the DPP HIV 1/2 (red line) and DPP HIV 1/2 IgM assays (blue line). Framed is the same data subset as that included in panel A but results are shown in percent signal reduction over time.

Article Snippet: DPP HIV 1/2 and DPP HIV-Syphilis tests were converted into investigational IgM-detecting assays, named DPP HIV 1/2 IgM and DPP HIV-Syphilis IgM, respectively, by replacing SpA coupled to gold nanoparticles with mouse monoclonal antibody to human IgM (HyTest Ltd., Turku, Finland), a specific reagent for IgM detection validated in commercial DPP products ( , ).

Techniques: Generated

IgM antibody detection by SpA-based rapid point-of-care tests in HIV-1 seroconversion panels <xref ref-type= a " width="100%" height="100%">

Journal: Microbiology Spectrum

Article Title: Rapid Point-of-Care Tests Using Staphylococcal Protein A Can Detect Early IgM Responses in HIV-1 and Treponema pallidum Infections

doi: 10.1128/spectrum.03309-22

Figure Lengend Snippet: IgM antibody detection by SpA-based rapid point-of-care tests in HIV-1 seroconversion panels a

Article Snippet: DPP HIV 1/2 and DPP HIV-Syphilis tests were converted into investigational IgM-detecting assays, named DPP HIV 1/2 IgM and DPP HIV-Syphilis IgM, respectively, by replacing SpA coupled to gold nanoparticles with mouse monoclonal antibody to human IgM (HyTest Ltd., Turku, Finland), a specific reagent for IgM detection validated in commercial DPP products ( , ).

Techniques:

Antibody detection in AccuVert Syphilis Seroconversion Panel PSS901 and effect of sample pre-treatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the DPP HIV-Syphilis (red lines) and DPP HIV-Syphilis IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 06 to 09 collected during the initial IgM response (days 45 to 59 since 1st bleed), in which antibody detection with each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples (same as framed in panel A) tested by the DPP HIV-Syphilis (red line) and DPP HIV-Syphilis IgM assays (blue line).

Journal: Microbiology Spectrum

Article Title: Rapid Point-of-Care Tests Using Staphylococcal Protein A Can Detect Early IgM Responses in HIV-1 and Treponema pallidum Infections

doi: 10.1128/spectrum.03309-22

Figure Lengend Snippet: Antibody detection in AccuVert Syphilis Seroconversion Panel PSS901 and effect of sample pre-treatment with 0.1 M 2-ME. (A) Micro Reader reflectance values generated with the DPP HIV-Syphilis (red lines) and DPP HIV-Syphilis IgM assays (blue lines) are shown for samples tested before (solid lines) and after 2-ME treatment (dashed lines). Framed are test results for samples 06 to 09 collected during the initial IgM response (days 45 to 59 since 1st bleed), in which antibody detection with each of the two immunoassays was diminished after 2-ME treatment by at least 50% signal reduction. (B) Results show differentially evolving susceptibility to the 2-ME treatment of postseroconversion samples (same as framed in panel A) tested by the DPP HIV-Syphilis (red line) and DPP HIV-Syphilis IgM assays (blue line).

Article Snippet: DPP HIV 1/2 and DPP HIV-Syphilis tests were converted into investigational IgM-detecting assays, named DPP HIV 1/2 IgM and DPP HIV-Syphilis IgM, respectively, by replacing SpA coupled to gold nanoparticles with mouse monoclonal antibody to human IgM (HyTest Ltd., Turku, Finland), a specific reagent for IgM detection validated in commercial DPP products ( , ).

Techniques: Generated

IgM antibody detection by DPP HIV-Syphilis assay in active syphilis <xref ref-type= a " width="100%" height="100%">

Journal: Microbiology Spectrum

Article Title: Rapid Point-of-Care Tests Using Staphylococcal Protein A Can Detect Early IgM Responses in HIV-1 and Treponema pallidum Infections

doi: 10.1128/spectrum.03309-22

Figure Lengend Snippet: IgM antibody detection by DPP HIV-Syphilis assay in active syphilis a

Article Snippet: DPP HIV 1/2 and DPP HIV-Syphilis tests were converted into investigational IgM-detecting assays, named DPP HIV 1/2 IgM and DPP HIV-Syphilis IgM, respectively, by replacing SpA coupled to gold nanoparticles with mouse monoclonal antibody to human IgM (HyTest Ltd., Turku, Finland), a specific reagent for IgM detection validated in commercial DPP products ( , ).

Techniques:

Correlation between antibody levels detected by SpA or anti-IgM reagent in early HIV-1 seroconversion and syphilis samples. (A) Micro Reader reflectance values generated with the DPP HIV-1/2 and DPP HIV-1/2 IgM assays are shown for the HIV-1 seroconversion samples included in <xref ref-type=Table 1 , Groups 2 and 3 (circles), and matching preseroconversion members (triangles). (B) Micro Reader reflectance values generated for treponemal antibody levels with the DPP HIV-Syphilis and DPP HIV-Syphilis IgM assays are shown for the syphilis-positive samples listed in Table 2 (circles) and preseroconversion panel members (triangles). Horizontal and vertical dashed lines indicate cutoff values used for test interpretation: DPP HIV-1/2, 10 RLU; DPP HIV-1/2 IgM, 40 RLU; DPP HIV-Syphilis, 7 RLU; DPP HIV-Syphilis IgM, 60 RLU. " width="100%" height="100%">

Journal: Microbiology Spectrum

Article Title: Rapid Point-of-Care Tests Using Staphylococcal Protein A Can Detect Early IgM Responses in HIV-1 and Treponema pallidum Infections

doi: 10.1128/spectrum.03309-22

Figure Lengend Snippet: Correlation between antibody levels detected by SpA or anti-IgM reagent in early HIV-1 seroconversion and syphilis samples. (A) Micro Reader reflectance values generated with the DPP HIV-1/2 and DPP HIV-1/2 IgM assays are shown for the HIV-1 seroconversion samples included in Table 1 , Groups 2 and 3 (circles), and matching preseroconversion members (triangles). (B) Micro Reader reflectance values generated for treponemal antibody levels with the DPP HIV-Syphilis and DPP HIV-Syphilis IgM assays are shown for the syphilis-positive samples listed in Table 2 (circles) and preseroconversion panel members (triangles). Horizontal and vertical dashed lines indicate cutoff values used for test interpretation: DPP HIV-1/2, 10 RLU; DPP HIV-1/2 IgM, 40 RLU; DPP HIV-Syphilis, 7 RLU; DPP HIV-Syphilis IgM, 60 RLU.

Article Snippet: DPP HIV 1/2 and DPP HIV-Syphilis tests were converted into investigational IgM-detecting assays, named DPP HIV 1/2 IgM and DPP HIV-Syphilis IgM, respectively, by replacing SpA coupled to gold nanoparticles with mouse monoclonal antibody to human IgM (HyTest Ltd., Turku, Finland), a specific reagent for IgM detection validated in commercial DPP products ( , ).

Techniques: Generated

Cnot3 -deficient mice show impaired early B-cell differentiation that can be alleviated in part by the expression of Mzb1 . ( A ) Flow cytometric analysis of bone marrow B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect B220 + CD43 + pro-B, B220 int CD43 − late pre-B, and immature B cells as well as B220 hi CD43 − recirculating B cells in the living lymphocyte gate. ( B ) Further gating of pro-B cells to detect pre-pro-B (HSA − BP1 − ), early pro-B (HSA + BP1 − ) and late pro-B (HSA + BP1 + ) cells. ( C ) Statistical analysis of the total numbers of pro-B (pink) and pre-B (red) cells in Cnot3 +/+ RERT Cre (wild-type) and Cnot3 fl/fl RERT Cre (knockout) mice. Long horizontal bars indicate the mean, and short horizontal bars represent the SD of the mean. Statistical significance between wild type and knockout is assessed by an unpaired two-tail Student's t -test. ( D ) PCR analysis of genomic DNA from Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect the deletion status of Cnot3 . Cnot6 served as a loading control. ( E ) Flow cytometric analysis of splenic B cells to detect CD21 int CD23 + follicular B cells and CD21 hi CD23 − marginal zone B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice. ( F ) Flow cytometric analysis of bone marrow cells in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice to detect the B-cell compartment as described in A . ( G ) Flow cytometric analysis of the effects of a Mzb1 transgene expression ( Mzb1 tg ) on early B-cell differentiation in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice. ( H , I ) Gating of bone marrow B220 + CD43 + pro-B cells to detect HSA + BP1 − early pro-B and HSA + BP1 + late pro-B stages. ( J , K ) Statistical analysis of the total numbers of pre-B (red), recirculating B (blue), early pro-B (orange), and late pro-B (green) cells in Cnot3 +/+ mb1 Cre , Cnot3 fl/fl mb1 Cre , Cnot3 +l+ mb1 Cre Mzb1 tg , and Cnot3 fl/fl mb1 Cre Mzb1 tg mice. Numbers in the FACS profiles indicate the percentage of cells within the gated population. The data are representative of four or more independent experiments.

Journal: Genes & Development

Article Title: Interaction of CCR4–NOT with EBF1 regulates gene-specific transcription and mRNA stability in B lymphopoiesis

doi: 10.1101/gad.285452.116

Figure Lengend Snippet: Cnot3 -deficient mice show impaired early B-cell differentiation that can be alleviated in part by the expression of Mzb1 . ( A ) Flow cytometric analysis of bone marrow B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect B220 + CD43 + pro-B, B220 int CD43 − late pre-B, and immature B cells as well as B220 hi CD43 − recirculating B cells in the living lymphocyte gate. ( B ) Further gating of pro-B cells to detect pre-pro-B (HSA − BP1 − ), early pro-B (HSA + BP1 − ) and late pro-B (HSA + BP1 + ) cells. ( C ) Statistical analysis of the total numbers of pro-B (pink) and pre-B (red) cells in Cnot3 +/+ RERT Cre (wild-type) and Cnot3 fl/fl RERT Cre (knockout) mice. Long horizontal bars indicate the mean, and short horizontal bars represent the SD of the mean. Statistical significance between wild type and knockout is assessed by an unpaired two-tail Student's t -test. ( D ) PCR analysis of genomic DNA from Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice to detect the deletion status of Cnot3 . Cnot6 served as a loading control. ( E ) Flow cytometric analysis of splenic B cells to detect CD21 int CD23 + follicular B cells and CD21 hi CD23 − marginal zone B cells in Cnot3 +/+ RERT Cre and Cnot3 fl/fl RERT Cre mice. ( F ) Flow cytometric analysis of bone marrow cells in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice to detect the B-cell compartment as described in A . ( G ) Flow cytometric analysis of the effects of a Mzb1 transgene expression ( Mzb1 tg ) on early B-cell differentiation in Cnot3 +/+ mb1 Cre and Cnot3 fl/fl mb1 Cre mice. ( H , I ) Gating of bone marrow B220 + CD43 + pro-B cells to detect HSA + BP1 − early pro-B and HSA + BP1 + late pro-B stages. ( J , K ) Statistical analysis of the total numbers of pre-B (red), recirculating B (blue), early pro-B (orange), and late pro-B (green) cells in Cnot3 +/+ mb1 Cre , Cnot3 fl/fl mb1 Cre , Cnot3 +l+ mb1 Cre Mzb1 tg , and Cnot3 fl/fl mb1 Cre Mzb1 tg mice. Numbers in the FACS profiles indicate the percentage of cells within the gated population. The data are representative of four or more independent experiments.

Article Snippet: Anti-CD19 (1D3), BP1 (BP-1), CD25 (PC61) HSA (M1/69), CD43 (R2/60), B220 (RA-6B2), CD93 (AA4.1), CD23 (B3B4), CD21 (7G6), and α-IgMμ (Jackson ImmunoResearch, #115–175-075) were used.

Techniques: Cell Differentiation, Expressing, Knock-Out, Control