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  • 99
    Thermo Fisher immunoglobulin m igm
    Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific <t>immunoglobulin</t> M <t>(IgM)</t> and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P
    Immunoglobulin M Igm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore antihuman immunoglobulin m igm
    Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific <t>immunoglobulin</t> M <t>(IgM)</t> and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P
    Antihuman Immunoglobulin M Igm, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Focus Diagnostics Inc immunoglobulin m igm
    Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific <t>immunoglobulin</t> M <t>(IgM)</t> and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P
    Immunoglobulin M Igm, supplied by Focus Diagnostics Inc, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Abcam immunoglobulin m igm
    Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, <t>IgM,</t> PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.
    Immunoglobulin M Igm, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Pharmingen immunoglobulin m igm antibodies
    Induction of Ca 2+ mobilization and tyrosine phosphorylation in p110δ-deficient mice. (A) Induction of Ca 2+ was measured by flow cytometry following stimulation of Indo-1-labeled splenic B cells with <t>anti-IgM</t> or of splenic T cells with anti-CD3 followed by anti-IgG. Arrow 1, time point of specific stimulation; arrow 2, time point of ionomycin addition. The results are representative of those from four independent experiments. WT, wild type. (B) Purified splenic B cells from wild-type and p110δ −/− mice were stimulated with anti-IgM (20 μg/ml) for 3 min and analyzed for the induction of tyrosine phosphorylation (pTyr) of Btk and PLCγ2. IP, immunoprecipitation.
    Immunoglobulin M Igm Antibodies, supplied by Pharmingen, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    SouthernBiotech immunoglobulin m igm biotin
    Unimpaired lymphocyte development in ALX-deficient mice. Wild-type (WT) and ALX-deficient (ALX KO) littermates were analyzed at 8 weeks of age for developmental defects in T- and B-cell development by FACS analysis. (A) CD4 − CD8 − , CD4 + CD8 + , CD4 + , and CD8 + thymocyte populations were similar between wild-type and ALX-deficient mice. (B) Developing B cells in the bone marrow were gated for B220 + AA4.1 + (to exclude recirculating mature B cells) and further subdivided into pro-B-cell (CD43 + <t>IgM</t> − ), pre-B-cell (CD43 − IgM − ), and immature B-cell (CD43 − IgM + ) populations. No differences were observed for any of these populations, demonstrating that B-cell development proceeds normally in ALX-deficient mice. (C) Splenocytes stained with CD4 and CD8 revealed no differences in the proportions of CD4 and CD8 T cells in the periphery. To analyze mature B-cell populations, splenocytes were first gated for B220 + AA4.1 − (to exclude transitional cells) and further subdivided into IgM + CD21/35 + follicular B cells and IgM high CD21/35 high marginal-zone B cells. Again, no differences in the mature B-cell populations in terms of number or proportion were observed for ALX-deficient mice.
    Immunoglobulin M Igm Biotin, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Becton Dickinson immunoglobulin m igm
    Increased levels of serum immunoglobulin G1 (IgG1) in Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice. (a) Spontaneous increases in circulating serum IgG1 levels were detected in the sera of 8-week-old Pten +/– SHIP +/– mice, as compared to the other genotypes, and this pattern remained steady and elevated over time. (b) Serum levels of circulating <t>immunoglobulin</t> M <t>(IgM)</t> were not significantly increased over all genotypes at the 8-week point, but steadily increased, becoming significant at later time-points. (c) Levels of circulating immunoglobulin G2b (IgG2b) did not achieve statistical significance compared to all genotypes until the 24-week time-point. Values were representative of sera from at least nine animals per genotype, assessed in duplicate. Error bars represent the standard error of the mean (SEM) of the duplicate readings. * P
    Immunoglobulin M Igm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 80/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    SouthernBiotech immunoglobulin m igm
    Bortezomib-mediated long-lived plasma cell (LLPC) depletion is transient. Twelve- to 16-wk-old NZB/W F1 mice were treated with one bortezomib cycle consisting of two bortezomib injections within 36 h and analyzed 12 h and 3, 7 and 15 days after the last bortezomib injection. (A) Representative gating strategy for plasma cell analysis by flow cytometry. Lymphocytes were gated according to their position in forward and sideward scatter, and doublets were excluded from the analysis (not shown). Plasma cells were identified as CD138+ and intracellular kappa light chain + cells both in spleen and bone marrow. Within the plasma cells population, plasmablasts and newly generated plasma cells were identified as cells with a higher expression of MHC-II. Conversely, MHC-II low plasma cells were regarded as LLPCs. A representative plot of the expression of MHC-II on the plasma cell population is shown for spleen and bone marrow 15 days after bortezomib treatment. (B) Absolute numbers of all CD138+/intracellular kappa light chain + plasma cells (white bars) and the subgroups of short-lived, MHC high (gray bars) and long-lived, MHC-II low (black bars) plasma cells in spleens (left) and bone marrows (right) 12 h (0.5 days), 3, 7 and 15 days after one cycle of bortezomib measured by flow cytometry. n = 7 mice per time point. (C) Frequencies of the remaining anti-double-stranded DNA (dsDNA) antibody-secreting cells (ASCs) (immunoglobulin G (IgG) and <t>immunoglobulin</t> M <t>(IgM)</t> combined) in spleen (left) and bone marrow (right) calculated by comparison with untreated mice (day 0), as detected by enzyme-linked immunospot (ELISPOT). n = 4 mice per time point. Data are presented as mean and standard error of the mean (SEM). n.s., nonsignificant. P > 0.05, * P ≤0.05, ** P ≤0.01 by two-tailed unpaired t test.
    Immunoglobulin M Igm, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 80/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Fuller Laboratories immunoglobulin m igm
    Bortezomib-mediated long-lived plasma cell (LLPC) depletion is transient. Twelve- to 16-wk-old NZB/W F1 mice were treated with one bortezomib cycle consisting of two bortezomib injections within 36 h and analyzed 12 h and 3, 7 and 15 days after the last bortezomib injection. (A) Representative gating strategy for plasma cell analysis by flow cytometry. Lymphocytes were gated according to their position in forward and sideward scatter, and doublets were excluded from the analysis (not shown). Plasma cells were identified as CD138+ and intracellular kappa light chain + cells both in spleen and bone marrow. Within the plasma cells population, plasmablasts and newly generated plasma cells were identified as cells with a higher expression of MHC-II. Conversely, MHC-II low plasma cells were regarded as LLPCs. A representative plot of the expression of MHC-II on the plasma cell population is shown for spleen and bone marrow 15 days after bortezomib treatment. (B) Absolute numbers of all CD138+/intracellular kappa light chain + plasma cells (white bars) and the subgroups of short-lived, MHC high (gray bars) and long-lived, MHC-II low (black bars) plasma cells in spleens (left) and bone marrows (right) 12 h (0.5 days), 3, 7 and 15 days after one cycle of bortezomib measured by flow cytometry. n = 7 mice per time point. (C) Frequencies of the remaining anti-double-stranded DNA (dsDNA) antibody-secreting cells (ASCs) (immunoglobulin G (IgG) and <t>immunoglobulin</t> M <t>(IgM)</t> combined) in spleen (left) and bone marrow (right) calculated by comparison with untreated mice (day 0), as detected by enzyme-linked immunospot (ELISPOT). n = 4 mice per time point. Data are presented as mean and standard error of the mean (SEM). n.s., nonsignificant. P > 0.05, * P ≤0.05, ** P ≤0.01 by two-tailed unpaired t test.
    Immunoglobulin M Igm, supplied by Fuller Laboratories, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Jackson Immuno anti immunoglobulin m igm
    T-oligo treatment does not cause apoptosis in normal primary B or T cells. ( a ) Primary B-2 cells were purified from mouse spleens, cultured and stimulated with 1 µg/ml goat anti-mouse <t>IgM</t> F(ab′) 2 antibody (μ chain specific), 1
    Anti Immunoglobulin M Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bethyl immunoglobulin m igm
    The mean (±SD) serum IgG, <t>IgM</t> and IgA concentration of sows in different experimental groups; * – statistical difference compared to control group
    Immunoglobulin M Igm, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Agilent technologies immunoglobulin m igm
    <t>AQP4-negative</t> GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and <t>IgM-positive</t> (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).
    Immunoglobulin M Igm, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 82/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Diatec immunoglobulin m igm
    <t>AQP4-negative</t> GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and <t>IgM-positive</t> (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).
    Immunoglobulin M Igm, supplied by Diatec, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    CTK Biotech anti hav immunoglobulin m igm
    <t>AQP4-negative</t> GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and <t>IgM-positive</t> (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).
    Anti Hav Immunoglobulin M Igm, supplied by CTK Biotech, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Immunology Consultant Laboratory immunoglobulin m igm capture antibody
    <t>AQP4-negative</t> GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and <t>IgM-positive</t> (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).
    Immunoglobulin M Igm Capture Antibody, supplied by Immunology Consultant Laboratory, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Jackson Immuno immunoglobulin m igm fitc conjugate
    <t>AQP4-negative</t> GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and <t>IgM-positive</t> (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).
    Immunoglobulin M Igm Fitc Conjugate, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher immunoglobulin m igm fragmentation kit
    <t>AQP4-negative</t> GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and <t>IgM-positive</t> (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).
    Immunoglobulin M Igm Fragmentation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Becton Dickinson mouse normal immunoglobulin m igm
    <t>AQP4-negative</t> GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and <t>IgM-positive</t> (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).
    Mouse Normal Immunoglobulin M Igm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Jackson Immuno antihuman immunoglobulin m igm hrp
    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific <t>immunoglobulin</t> M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; <t>IgM,</t> immunoglobulin M; TCID 50 , 50% tissue culture infective dose.
    Antihuman Immunoglobulin M Igm Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Beckman Coulter anti gb3 cd77 immunoglobulin m igm
    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific <t>immunoglobulin</t> M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; <t>IgM,</t> immunoglobulin M; TCID 50 , 50% tissue culture infective dose.
    Anti Gb3 Cd77 Immunoglobulin M Igm, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory anti immunoglobulin m anti igm antibody
    GDC-0853 represses NF-κB–dependent gene transcription. CLL patient cells were treated with 1 µM BTK inhibitor for 72 hours with or without <t>anti-IgM</t> stimulation and assessed for gene expression changes of NF-κB targets by RT-PCR (N = 8). ** P ≤ .01; *** P ≤ .001.
    Anti Immunoglobulin M Anti Igm Antibody, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti mouse immunoglobulin m igm
    GDC-0853 represses NF-κB–dependent gene transcription. CLL patient cells were treated with 1 µM BTK inhibitor for 72 hours with or without <t>anti-IgM</t> stimulation and assessed for gene expression changes of NF-κB targets by RT-PCR (N = 8). ** P ≤ .01; *** P ≤ .001.
    Anti Mouse Immunoglobulin M Igm, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech human α immunoglobulin m α igm
    GDC-0853 represses NF-κB–dependent gene transcription. CLL patient cells were treated with 1 µM BTK inhibitor for 72 hours with or without <t>anti-IgM</t> stimulation and assessed for gene expression changes of NF-κB targets by RT-PCR (N = 8). ** P ≤ .01; *** P ≤ .001.
    Human α Immunoglobulin M α Igm, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore immunoglobulin m igm secondary antibodies
    Antibody dynamics of Salmonella enterica serovar Typhimurium–specific antibodies (ie, O4) and S . enterica serovar Enteritidis–specific antibodies (ie, O9) during the first 12 months of life in infants from Ho Chi Minh City, Vietnam. Plots show a generalized additive mixed-effect model–fitted population trend (solid black line) for anti-O4 <t>immunoglobulin</t> M <t>(IgM;</t> A ), anti-O4 immunoglobulin G (IgG; B ), anti-O9 IgM ( C ), and anti-O9 IgG ( D ) from birth through 12 months of age. 95% confidence intervals are shown by the dashed black lines.
    Immunoglobulin M Igm Secondary Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno igm
    (a) B-cell proliferation upon stimulation with CpG. Data representative of at least three different experiments. (b) Antibody production after in vitro CpG stimulation of peripheral blood mononuclear cells. Antibodies of <t>IgM,</t> <t>IgA</t> and IgG isotype were
    Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    igm  (Abcam)
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    Abcam igm
    Binding of serum immunoglobulins and C192S. Human sera were passed through an affinity column immobilized with C192S, and then the binding ligands of C192S were eluted and the fraction 13 (F13) was collected. The contents in diluted human serum, different amount of purified human immunoglobulins, and F13 (1 µg) were verified using 12% <t>SDS-PAGE</t> (A) and blotted with goat anti-human IgG (B), <t>IgM</t> (C), or IgA (D). (E) 2 µM of purified C192S or BSA was coated in a 96-well ELISA plate, and binding of IgG, IgM, or IgA with C192S or BSA was detected by ELISA, as described in Materials and Methods. ** P
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    igm  (Bio-Rad)
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    Bio-Rad igm
    Assessment of the nonimmune Ig-binding properties of 3D7 VAR2CSA domains by FACS. ( A ) Domain structure of 3D7 VAR2CSA. <t>IgG-</t> and <t>IgM-binding</t> domains as well as previously identified CSA-binding domains are indicated. ( B ) Data shown are flow-cytometric
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    Proteintech control immunoglobulin m
    Assessment of the nonimmune Ig-binding properties of 3D7 VAR2CSA domains by FACS. ( A ) Domain structure of 3D7 VAR2CSA. <t>IgG-</t> and <t>IgM-binding</t> domains as well as previously identified CSA-binding domains are indicated. ( B ) Data shown are flow-cytometric
    Control Immunoglobulin M, supplied by Proteintech, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Diagast anti d rh1 igm1
    Assessment of the nonimmune Ig-binding properties of 3D7 VAR2CSA domains by FACS. ( A ) Domain structure of 3D7 VAR2CSA. <t>IgG-</t> and <t>IgM-binding</t> domains as well as previously identified CSA-binding domains are indicated. ( B ) Data shown are flow-cytometric
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    Bio-Synthesis Inc anti immunoglobulin m igm
    Assessment of the nonimmune Ig-binding properties of 3D7 VAR2CSA domains by FACS. ( A ) Domain structure of 3D7 VAR2CSA. <t>IgG-</t> and <t>IgM-binding</t> domains as well as previously identified CSA-binding domains are indicated. ( B ) Data shown are flow-cytometric
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    Image Search Results


    Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P

    Journal: The Journal of Infectious Diseases

    Article Title: Early Appearance of Neutralizing Immunoglobulin G3 Antibodies Is Associated With Chikungunya Virus Clearance and Long-term Clinical Protection

    doi: 10.1093/infdis/jis033

    Figure Lengend Snippet: Antibody (Ab) profiles of chikungunya virus (CHIKV)–infected patients. A , Virus-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody titers in plasma samples (n = 30) at a dilution of 1:2000 were determined by enzyme-linked immunosorbent assay (ELISA) using purified CHIKV virions. B , Detection of CHIKV by plasma from CHIKV-infected patients. HEK 293T cells were infected with CHIKV (SGP11) at a multiplicity of infection of 10, fixed at 6 h postinfection, and stained with 2 representative patients’ plasma at 2–3 months post–illness onset (PIO) using a dilution of 1:500. Healthy plasma was used as a control. CHIKV antigen was detected by antihuman IgG antibody conjugated to Alexa Fluor 488 (green). 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus. Scale bar: 10 μm. C , Virus-specific IgG isotype titers in plasma samples. Immunoglobulin G1 (IgG1) (□), immunoglobulin G2 (IgG2) (Δ), immunoglobulin G3 (IgG3) (•), or immunoglobulin G4 (IgG4) (⋄) Abs were determined as in ( A ) using specific secondary antibodies. Each point represents the mean of 30 patients’ samples from the cohort. D , Chikungunya virion–based ELISA was used to determine virus-specific IgG isotype titers in plasma samples (median, 10 d PIO; n = 30) at a dilution of 1:100. Anti-CHIKV IgG1, IgG2, IgG3, or IgG4 Abs were determined using specific secondary Abs. Data are presented as mean ± standard error of the mean (SEM) of 30 patients’ samples and are representative of 2 independent experiments with similar results. E , Profile of IgG3 levels at different times PIO in early (n = 16) and late IgG3 responders (n = 14) according to the pattern of IgG3 titer at median, 10 d PIO. Data are presented as mean ± SEM. Data are representative of 2 independent experiments with similar results. Statistical significance was measured using Mann–Whitney U test. ** P

    Article Snippet: Horseradish peroxidase–conjugated mouse antihuman immunoglobulin G (IgG), immunoglobulin G1 (IgG1), immunoglobulin G2 (IgG2), IgG3, immunoglobulin G4 (IgG4), and immunoglobulin M (IgM) (Molecular Probes) were used to detect human antibodies bound to virus-coated wells.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Purification, Staining, MANN-WHITNEY

    Immunoglobulin production by activated human B lymphocytes in vitro is suppressed by Acthar. IgG ( top panel ) and IgM ( bottom panel ) were measured in supernatants from human peripheral B cells cultured for 6 days under basal conditions, with added IL-4/CD40L alone, or with IL-4/CD40L plus added Acthar at ACTH analog concentrations of approximately 0.124, 1.24, or 2.49 μM ( stepped bars ). Parallel cultures contained IL-4/CD40L plus matched volumes of placebo. Supernatants were harvested and assayed for IgG or IgM concentration and results analyzed by ANOVA (Kruskal–Wallis test, p

    Journal: Arthritis Research & Therapy

    Article Title: Direct effects of HP Acthar Gel® on human B lymphocyte activation in vitro

    doi: 10.1186/s13075-015-0823-y

    Figure Lengend Snippet: Immunoglobulin production by activated human B lymphocytes in vitro is suppressed by Acthar. IgG ( top panel ) and IgM ( bottom panel ) were measured in supernatants from human peripheral B cells cultured for 6 days under basal conditions, with added IL-4/CD40L alone, or with IL-4/CD40L plus added Acthar at ACTH analog concentrations of approximately 0.124, 1.24, or 2.49 μM ( stepped bars ). Parallel cultures contained IL-4/CD40L plus matched volumes of placebo. Supernatants were harvested and assayed for IgG or IgM concentration and results analyzed by ANOVA (Kruskal–Wallis test, p

    Article Snippet: Assays for immunoglobulins in cell culture supernatants Immunoglobulin G (IgG) and immunoglobulin M (IgM) levels in cell culture supernatants were measured using immunoglobulin heavy chain-specific (IgG) and intact molecule-specific (IgM) microsphere agglutination assays (Pierce Easy Titer; Thermo-Fisher, Rockford, IL, USA).

    Techniques: In Vitro, Cell Culture, Concentration Assay

    Expression of PI3Kγ subunits in malignant B cell lines and CLL patient samples. a , b CLL cells were stimulated with F(ab′) 2 α-IgM (10 µg/ml) or CD40L/IL-4 (50 ng/ml each) and harvested 24 h later for RNA extraction and RT-qPCR analysis. mRNA expression of a p110γ and b p101 were determined, and expression levels were normalized against the expression of TATA box-binding protein (TBP). Patients were divided into indolent vs. progressive groups based on IgVH mutation status. c Protein expression of p110γ and p101 in CLL samples in response to BCR stimulation or CD40L/IL-4 stimulation. Data are representative of nine patients analyzed

    Journal: Leukemia

    Article Title: Distinct roles for phosphoinositide 3-kinases γ and δ in malignant B cell migration

    doi: 10.1038/s41375-018-0012-5

    Figure Lengend Snippet: Expression of PI3Kγ subunits in malignant B cell lines and CLL patient samples. a , b CLL cells were stimulated with F(ab′) 2 α-IgM (10 µg/ml) or CD40L/IL-4 (50 ng/ml each) and harvested 24 h later for RNA extraction and RT-qPCR analysis. mRNA expression of a p110γ and b p101 were determined, and expression levels were normalized against the expression of TATA box-binding protein (TBP). Patients were divided into indolent vs. progressive groups based on IgVH mutation status. c Protein expression of p110γ and p101 in CLL samples in response to BCR stimulation or CD40L/IL-4 stimulation. Data are representative of nine patients analyzed

    Article Snippet: Briefly, 5 million CLL cells per treatment group were stimulated for 24 h with α-IgM or CD40L/IL-4, collected in TRIzol, and total RNA was isolated and purified using the Purelink RNA kit (Thermo Fisher). qPCR was performed using a LightCycler 96 PCR instrument (Roche Diagnostics) and Power SYBR green (Applied Biosystems).

    Techniques: Expressing, RNA Extraction, Quantitative RT-PCR, Binding Assay, Mutagenesis

    Regression is not caused by changes in the rate of proliferation of tumor cells. (A) B220 low cells of Eμ- myc and WT mice were stained for IgM and examined for BrdU incorporation and DNA content by flow cytometry. Eμ- myc mice at 33 and

    Journal: Blood

    Article Title: ATM-dependent spontaneous regression of early Eμ-myc–induced murine B-cell leukemia depends on natural killer and T cells

    doi: 10.1182/blood-2012-08-449025

    Figure Lengend Snippet: Regression is not caused by changes in the rate of proliferation of tumor cells. (A) B220 low cells of Eμ- myc and WT mice were stained for IgM and examined for BrdU incorporation and DNA content by flow cytometry. Eμ- myc mice at 33 and

    Article Snippet: Tumor cells were stained with combinations of B220-PerCP and immunoglobulin M (IgM) Ag-presenting cell or IgM-fluorescein isothiocyanate–specific antibodies (eBioscience).

    Techniques: Mouse Assay, Staining, BrdU Incorporation Assay, Flow Cytometry, Cytometry

    Spontaneous regression of B220 low cells in the blood of Eμ- myc mice. (A) Blood tumor load was assessed by flow cytometry. Dot plots are representative of blood stained for B220 and IgM expression. Oval gates identify B220 low populations in Eμ-

    Journal: Blood

    Article Title: ATM-dependent spontaneous regression of early Eμ-myc–induced murine B-cell leukemia depends on natural killer and T cells

    doi: 10.1182/blood-2012-08-449025

    Figure Lengend Snippet: Spontaneous regression of B220 low cells in the blood of Eμ- myc mice. (A) Blood tumor load was assessed by flow cytometry. Dot plots are representative of blood stained for B220 and IgM expression. Oval gates identify B220 low populations in Eμ-

    Article Snippet: Tumor cells were stained with combinations of B220-PerCP and immunoglobulin M (IgM) Ag-presenting cell or IgM-fluorescein isothiocyanate–specific antibodies (eBioscience).

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Staining, Expressing

    Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, IgM, PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.

    Journal: PLoS ONE

    Article Title: Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis

    doi: 10.1371/journal.pone.0118527

    Figure Lengend Snippet: Validation of selected candidate biomarkers for HCC development in a larger patient population. (A) Quantification of plasma concentration of CPN (pg/mg plasma protein), CP, IgM, PON1 (μg/mg plasma protein), C4a (ng/mg plasma protein) and FGA (μg/mg plasma protein) by ELISA. The sample size was 24 in the control group and 22 in the tumor group. * Statistically significant differences between groups. Data as mean ± standard error; (B) ROC curves showing that the best predictive ability to identify patients with HCC belongs to the combination of C4a, FGA, CP and PON1.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) ELISA tests were used to quantify the protein concentration of differentially expressed proteins CPN, CP, complement component 4a (C4a), fibrinogen-alpha (FGA), PON1 (USCN Life Science Inc., Wuhan, China), and immunoglobulin M (IgM) (Abcam, Cambridge, UK) in plasma samples from the control (24 cases) and tumor (22 cases) groups.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    Induction of Ca 2+ mobilization and tyrosine phosphorylation in p110δ-deficient mice. (A) Induction of Ca 2+ was measured by flow cytometry following stimulation of Indo-1-labeled splenic B cells with anti-IgM or of splenic T cells with anti-CD3 followed by anti-IgG. Arrow 1, time point of specific stimulation; arrow 2, time point of ionomycin addition. The results are representative of those from four independent experiments. WT, wild type. (B) Purified splenic B cells from wild-type and p110δ −/− mice were stimulated with anti-IgM (20 μg/ml) for 3 min and analyzed for the induction of tyrosine phosphorylation (pTyr) of Btk and PLCγ2. IP, immunoprecipitation.

    Journal: Molecular and Cellular Biology

    Article Title: Essential, Nonredundant Role for the Phosphoinositide 3-Kinase p110? in Signaling by the B-Cell Receptor Complex

    doi: 10.1128/MCB.22.24.8580-8591.2002

    Figure Lengend Snippet: Induction of Ca 2+ mobilization and tyrosine phosphorylation in p110δ-deficient mice. (A) Induction of Ca 2+ was measured by flow cytometry following stimulation of Indo-1-labeled splenic B cells with anti-IgM or of splenic T cells with anti-CD3 followed by anti-IgG. Arrow 1, time point of specific stimulation; arrow 2, time point of ionomycin addition. The results are representative of those from four independent experiments. WT, wild type. (B) Purified splenic B cells from wild-type and p110δ −/− mice were stimulated with anti-IgM (20 μg/ml) for 3 min and analyzed for the induction of tyrosine phosphorylation (pTyr) of Btk and PLCγ2. IP, immunoprecipitation.

    Article Snippet: Monoclonal antibodies (MAbs) conjugated to either fluorescein isothiocyanate (FITC), phycoerythrin (PE), or CyChrome and specific to murine B220/CD45R, immunoglobulin M (IgM), IgD, CD5, CD43, Thy1.2, interleukin-2 (IL-2) receptor (CD25), CD44, CD4, CD8, Mac-1 (CD11b), and Gr-1 (all from PharMingen) were used to label 106 cells for 30 min on ice.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Labeling, Purification, Immunoprecipitation

    Mycobacterium avium infection induces increased numbers of B cells expressing B7 molecules. B7.1and B7.2 expression on the surface of IgM + and IgG + B cells was performed by flow cytometry after mycobacterial infection or 0·04% Tween-80 in saline injection as control. There was a statistically significant increase in the number of both IgM + and IgG + B cells that express costimulatory molecules from B7 family.

    Journal: Immunology

    Article Title: A role for CD45RBlow CD38+ T cells and costimulatory pathways of T-cell activation in protection of non-obese diabetic (NOD) mice from diabetes

    doi: 10.1046/j.1365-2567.1999.00725.x

    Figure Lengend Snippet: Mycobacterium avium infection induces increased numbers of B cells expressing B7 molecules. B7.1and B7.2 expression on the surface of IgM + and IgG + B cells was performed by flow cytometry after mycobacterial infection or 0·04% Tween-80 in saline injection as control. There was a statistically significant increase in the number of both IgM + and IgG + B cells that express costimulatory molecules from B7 family.

    Article Snippet: Monoclonal antibodies (mAb) used in the flow cytometry analysis of splenic lymphocytes of the mice were the following: phycoerythrin (PE)-labelled anti-CD4, anti-CD3, anti-CD8, anti-CD80, anti-CD86, anti-CD40L and anti-CTLA-4; fluorescein isothiocyanate (FITC)-labelled anti-CD8, anti-CD4, anti-immunoglobulin M (IgM), anti-CD45RB and anti-hamster; cychrome-labelled anti-CD4 and purified hamster anti-mouse CD28 all purchased from PharMingen Inc. (San Diego, CA); FITC-labelled anti-IgG was purchased from Southern Biotechnology (Birmingham, AL).

    Techniques: Infection, Expressing, Flow Cytometry, Cytometry, Injection

    IgM + B cells from infected mice show increased expression of B7.2 molecules. B7.1 and B7.2 expression on B cells from infected and control mice was compared by flow cytometry. IgM + B cells from infected NOD mice had increased levels of B7.2 expression, whereas on IgG + B cells the expression of this molecule was decreased.

    Journal: Immunology

    Article Title: A role for CD45RBlow CD38+ T cells and costimulatory pathways of T-cell activation in protection of non-obese diabetic (NOD) mice from diabetes

    doi: 10.1046/j.1365-2567.1999.00725.x

    Figure Lengend Snippet: IgM + B cells from infected mice show increased expression of B7.2 molecules. B7.1 and B7.2 expression on B cells from infected and control mice was compared by flow cytometry. IgM + B cells from infected NOD mice had increased levels of B7.2 expression, whereas on IgG + B cells the expression of this molecule was decreased.

    Article Snippet: Monoclonal antibodies (mAb) used in the flow cytometry analysis of splenic lymphocytes of the mice were the following: phycoerythrin (PE)-labelled anti-CD4, anti-CD3, anti-CD8, anti-CD80, anti-CD86, anti-CD40L and anti-CTLA-4; fluorescein isothiocyanate (FITC)-labelled anti-CD8, anti-CD4, anti-immunoglobulin M (IgM), anti-CD45RB and anti-hamster; cychrome-labelled anti-CD4 and purified hamster anti-mouse CD28 all purchased from PharMingen Inc. (San Diego, CA); FITC-labelled anti-IgG was purchased from Southern Biotechnology (Birmingham, AL).

    Techniques: Infection, Mouse Assay, Expressing, Flow Cytometry, Cytometry

    Unimpaired lymphocyte development in ALX-deficient mice. Wild-type (WT) and ALX-deficient (ALX KO) littermates were analyzed at 8 weeks of age for developmental defects in T- and B-cell development by FACS analysis. (A) CD4 − CD8 − , CD4 + CD8 + , CD4 + , and CD8 + thymocyte populations were similar between wild-type and ALX-deficient mice. (B) Developing B cells in the bone marrow were gated for B220 + AA4.1 + (to exclude recirculating mature B cells) and further subdivided into pro-B-cell (CD43 + IgM − ), pre-B-cell (CD43 − IgM − ), and immature B-cell (CD43 − IgM + ) populations. No differences were observed for any of these populations, demonstrating that B-cell development proceeds normally in ALX-deficient mice. (C) Splenocytes stained with CD4 and CD8 revealed no differences in the proportions of CD4 and CD8 T cells in the periphery. To analyze mature B-cell populations, splenocytes were first gated for B220 + AA4.1 − (to exclude transitional cells) and further subdivided into IgM + CD21/35 + follicular B cells and IgM high CD21/35 high marginal-zone B cells. Again, no differences in the mature B-cell populations in terms of number or proportion were observed for ALX-deficient mice.

    Journal: Molecular and Cellular Biology

    Article Title: Negative Regulation of Interleukin-2 and p38 Mitogen-Activated Protein Kinase during T-Cell Activation by the Adaptor ALX

    doi: 10.1128/MCB.02067-05

    Figure Lengend Snippet: Unimpaired lymphocyte development in ALX-deficient mice. Wild-type (WT) and ALX-deficient (ALX KO) littermates were analyzed at 8 weeks of age for developmental defects in T- and B-cell development by FACS analysis. (A) CD4 − CD8 − , CD4 + CD8 + , CD4 + , and CD8 + thymocyte populations were similar between wild-type and ALX-deficient mice. (B) Developing B cells in the bone marrow were gated for B220 + AA4.1 + (to exclude recirculating mature B cells) and further subdivided into pro-B-cell (CD43 + IgM − ), pre-B-cell (CD43 − IgM − ), and immature B-cell (CD43 − IgM + ) populations. No differences were observed for any of these populations, demonstrating that B-cell development proceeds normally in ALX-deficient mice. (C) Splenocytes stained with CD4 and CD8 revealed no differences in the proportions of CD4 and CD8 T cells in the periphery. To analyze mature B-cell populations, splenocytes were first gated for B220 + AA4.1 − (to exclude transitional cells) and further subdivided into IgM + CD21/35 + follicular B cells and IgM high CD21/35 high marginal-zone B cells. Again, no differences in the mature B-cell populations in terms of number or proportion were observed for ALX-deficient mice.

    Article Snippet: The following fluorescence-activated cell sorter (FACS) reagents were used: B220-allophycocyanin (APC)-Cy7 (eBiosciences), AA4.1-APC (eBiosciences), CD43-fluorescein isothiocyanate (FITC) (Pharmingen), immunoglobulin M (IgM)-biotin (Southern Biotechnology), streptavidin-peridinin chlorophyll a protein-Cy5.5 (Pharmingen), CD4-phycoerythrin (PE) (Caltag), CD8-APC (Caltag), CD44-FITC (Pharmingen), CD62L-PE (Pharmingen), CD4-biotin (Pharmingen), CD25-APC (Pharmingen), and CD69-FITC (Pharmingen).

    Techniques: Mouse Assay, FACS, Staining

    Intact T-cell-dependent B-cell responses to antigen in vivo. (A) Serum immunoglobulin levels from wild-type and ALX-deficient mice were analyzed by ELISA. The data represent averages for six wild-type and nine ALX-deficient mice between 12 and 15 weeks of age. The error bars reflect the standard deviations within each group. (B) Five wild-type and four ALX-deficient mice between 9 and 10 weeks of age were immunized with 100 μg alum-precipitated NP-KLH/mouse on day 0, boosted on day 47, and sacrificed on day 54. Sera were collected at the time points shown and were assayed for the concentration of high affinity anti-NP IgG compared to that of the NP-specific IgG1 monoclonal antibody B1-8 used as a standard. The points represent the average concentrations for all wild-type mice or ALX-deficient mice. Error bars reflect the standard deviations within each genotype. (C) Purified B cells from wild-type and ALX-deficient mice were labeled with CSFE and either were left unstimulated or were stimulated with a combination of anti-IgM, anti-CD40, BLyS, CpG, and/or LPS as shown in the figure and as described in Materials and Methods. After 3 days, the cells were analyzed by FACS. TOPRO-3 (100 nM) was added to exclude dead cells from further analysis. A fixed number of 6-μm beads were added to each sample to permit calculation of the absolute number of live cells within each sample. The y axis represents absolute numbers of live B cells. For each stimulation condition, the gray-filled histograms represent wild-type B cells and the solid black lines represent ALX-deficient B cells. The results shown are representative of three separate experiments. WT, wild type; ALX KO, ALX deficient.

    Journal: Molecular and Cellular Biology

    Article Title: Negative Regulation of Interleukin-2 and p38 Mitogen-Activated Protein Kinase during T-Cell Activation by the Adaptor ALX

    doi: 10.1128/MCB.02067-05

    Figure Lengend Snippet: Intact T-cell-dependent B-cell responses to antigen in vivo. (A) Serum immunoglobulin levels from wild-type and ALX-deficient mice were analyzed by ELISA. The data represent averages for six wild-type and nine ALX-deficient mice between 12 and 15 weeks of age. The error bars reflect the standard deviations within each group. (B) Five wild-type and four ALX-deficient mice between 9 and 10 weeks of age were immunized with 100 μg alum-precipitated NP-KLH/mouse on day 0, boosted on day 47, and sacrificed on day 54. Sera were collected at the time points shown and were assayed for the concentration of high affinity anti-NP IgG compared to that of the NP-specific IgG1 monoclonal antibody B1-8 used as a standard. The points represent the average concentrations for all wild-type mice or ALX-deficient mice. Error bars reflect the standard deviations within each genotype. (C) Purified B cells from wild-type and ALX-deficient mice were labeled with CSFE and either were left unstimulated or were stimulated with a combination of anti-IgM, anti-CD40, BLyS, CpG, and/or LPS as shown in the figure and as described in Materials and Methods. After 3 days, the cells were analyzed by FACS. TOPRO-3 (100 nM) was added to exclude dead cells from further analysis. A fixed number of 6-μm beads were added to each sample to permit calculation of the absolute number of live cells within each sample. The y axis represents absolute numbers of live B cells. For each stimulation condition, the gray-filled histograms represent wild-type B cells and the solid black lines represent ALX-deficient B cells. The results shown are representative of three separate experiments. WT, wild type; ALX KO, ALX deficient.

    Article Snippet: The following fluorescence-activated cell sorter (FACS) reagents were used: B220-allophycocyanin (APC)-Cy7 (eBiosciences), AA4.1-APC (eBiosciences), CD43-fluorescein isothiocyanate (FITC) (Pharmingen), immunoglobulin M (IgM)-biotin (Southern Biotechnology), streptavidin-peridinin chlorophyll a protein-Cy5.5 (Pharmingen), CD4-phycoerythrin (PE) (Caltag), CD8-APC (Caltag), CD44-FITC (Pharmingen), CD62L-PE (Pharmingen), CD4-biotin (Pharmingen), CD25-APC (Pharmingen), and CD69-FITC (Pharmingen).

    Techniques: In Vivo, Mouse Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Purification, Labeling, FACS

    Increased levels of serum immunoglobulin G1 (IgG1) in Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice. (a) Spontaneous increases in circulating serum IgG1 levels were detected in the sera of 8-week-old Pten +/– SHIP +/– mice, as compared to the other genotypes, and this pattern remained steady and elevated over time. (b) Serum levels of circulating immunoglobulin M (IgM) were not significantly increased over all genotypes at the 8-week point, but steadily increased, becoming significant at later time-points. (c) Levels of circulating immunoglobulin G2b (IgG2b) did not achieve statistical significance compared to all genotypes until the 24-week time-point. Values were representative of sera from at least nine animals per genotype, assessed in duplicate. Error bars represent the standard error of the mean (SEM) of the duplicate readings. * P

    Journal: Immunology

    Article Title: Compound heterozygosity for Pten and SHIP augments T-dependent humoral immune responses and cytokine production by CD4+ T cells

    doi: 10.1111/j.1365-2567.2004.01901.x

    Figure Lengend Snippet: Increased levels of serum immunoglobulin G1 (IgG1) in Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice. (a) Spontaneous increases in circulating serum IgG1 levels were detected in the sera of 8-week-old Pten +/– SHIP +/– mice, as compared to the other genotypes, and this pattern remained steady and elevated over time. (b) Serum levels of circulating immunoglobulin M (IgM) were not significantly increased over all genotypes at the 8-week point, but steadily increased, becoming significant at later time-points. (c) Levels of circulating immunoglobulin G2b (IgG2b) did not achieve statistical significance compared to all genotypes until the 24-week time-point. Values were representative of sera from at least nine animals per genotype, assessed in duplicate. Error bars represent the standard error of the mean (SEM) of the duplicate readings. * P

    Article Snippet: ELISA quantification of serum immunoglobulin levels was performed, as previously described, using capture and biotinylated detection antibodies for immunoglobulin G1 (IgG1), immunoglobulin G2b (IgG2b) and immunoglobulin M (IgM) (BD Pharmingen).

    Techniques: Mouse Assay

    Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice show an increased humoral response to the T-dependent antigen, nitrophenylacetyl (NP)-keyhole limpet haemocyanin (NP-KLH). Serum antibodies against NP-bovine serum albumin (NP-BSA) were assessed from six or seven mice of each genotype that had been challenged with NP-Ficoll or NP-KLH. Anti-NP titres [immunoglobulin M (IgM) and pan immunoglobulin G (IgG)] were determined by enzyme-linked immunosorbent assay (ELISA) on serially diluted serum samples. Bars indicate the average titre, and triangles represent individual mice. No differences were observed in either the IgM or IgG response to NP-Ficoll (a, b). However, statistically significant differences in IgM and IgG responses to NP-KLH were detected (c, d). * P

    Journal: Immunology

    Article Title: Compound heterozygosity for Pten and SHIP augments T-dependent humoral immune responses and cytokine production by CD4+ T cells

    doi: 10.1111/j.1365-2567.2004.01901.x

    Figure Lengend Snippet: Pten +/– SHIP +/– (phosphatase with tensin homology/SH2-containing inositol phosphatase) mice show an increased humoral response to the T-dependent antigen, nitrophenylacetyl (NP)-keyhole limpet haemocyanin (NP-KLH). Serum antibodies against NP-bovine serum albumin (NP-BSA) were assessed from six or seven mice of each genotype that had been challenged with NP-Ficoll or NP-KLH. Anti-NP titres [immunoglobulin M (IgM) and pan immunoglobulin G (IgG)] were determined by enzyme-linked immunosorbent assay (ELISA) on serially diluted serum samples. Bars indicate the average titre, and triangles represent individual mice. No differences were observed in either the IgM or IgG response to NP-Ficoll (a, b). However, statistically significant differences in IgM and IgG responses to NP-KLH were detected (c, d). * P

    Article Snippet: ELISA quantification of serum immunoglobulin levels was performed, as previously described, using capture and biotinylated detection antibodies for immunoglobulin G1 (IgG1), immunoglobulin G2b (IgG2b) and immunoglobulin M (IgM) (BD Pharmingen).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Bortezomib-mediated long-lived plasma cell (LLPC) depletion is transient. Twelve- to 16-wk-old NZB/W F1 mice were treated with one bortezomib cycle consisting of two bortezomib injections within 36 h and analyzed 12 h and 3, 7 and 15 days after the last bortezomib injection. (A) Representative gating strategy for plasma cell analysis by flow cytometry. Lymphocytes were gated according to their position in forward and sideward scatter, and doublets were excluded from the analysis (not shown). Plasma cells were identified as CD138+ and intracellular kappa light chain + cells both in spleen and bone marrow. Within the plasma cells population, plasmablasts and newly generated plasma cells were identified as cells with a higher expression of MHC-II. Conversely, MHC-II low plasma cells were regarded as LLPCs. A representative plot of the expression of MHC-II on the plasma cell population is shown for spleen and bone marrow 15 days after bortezomib treatment. (B) Absolute numbers of all CD138+/intracellular kappa light chain + plasma cells (white bars) and the subgroups of short-lived, MHC high (gray bars) and long-lived, MHC-II low (black bars) plasma cells in spleens (left) and bone marrows (right) 12 h (0.5 days), 3, 7 and 15 days after one cycle of bortezomib measured by flow cytometry. n = 7 mice per time point. (C) Frequencies of the remaining anti-double-stranded DNA (dsDNA) antibody-secreting cells (ASCs) (immunoglobulin G (IgG) and immunoglobulin M (IgM) combined) in spleen (left) and bone marrow (right) calculated by comparison with untreated mice (day 0), as detected by enzyme-linked immunospot (ELISPOT). n = 4 mice per time point. Data are presented as mean and standard error of the mean (SEM). n.s., nonsignificant. P > 0.05, * P ≤0.05, ** P ≤0.01 by two-tailed unpaired t test.

    Journal: Arthritis Research & Therapy

    Article Title: Long-lived plasma cells are early and constantly generated in New Zealand Black/New Zealand White F1 mice and their therapeutic depletion requires a combined targeting of autoreactive plasma cells and their precursors

    doi: 10.1186/s13075-015-0551-3

    Figure Lengend Snippet: Bortezomib-mediated long-lived plasma cell (LLPC) depletion is transient. Twelve- to 16-wk-old NZB/W F1 mice were treated with one bortezomib cycle consisting of two bortezomib injections within 36 h and analyzed 12 h and 3, 7 and 15 days after the last bortezomib injection. (A) Representative gating strategy for plasma cell analysis by flow cytometry. Lymphocytes were gated according to their position in forward and sideward scatter, and doublets were excluded from the analysis (not shown). Plasma cells were identified as CD138+ and intracellular kappa light chain + cells both in spleen and bone marrow. Within the plasma cells population, plasmablasts and newly generated plasma cells were identified as cells with a higher expression of MHC-II. Conversely, MHC-II low plasma cells were regarded as LLPCs. A representative plot of the expression of MHC-II on the plasma cell population is shown for spleen and bone marrow 15 days after bortezomib treatment. (B) Absolute numbers of all CD138+/intracellular kappa light chain + plasma cells (white bars) and the subgroups of short-lived, MHC high (gray bars) and long-lived, MHC-II low (black bars) plasma cells in spleens (left) and bone marrows (right) 12 h (0.5 days), 3, 7 and 15 days after one cycle of bortezomib measured by flow cytometry. n = 7 mice per time point. (C) Frequencies of the remaining anti-double-stranded DNA (dsDNA) antibody-secreting cells (ASCs) (immunoglobulin G (IgG) and immunoglobulin M (IgM) combined) in spleen (left) and bone marrow (right) calculated by comparison with untreated mice (day 0), as detected by enzyme-linked immunospot (ELISPOT). n = 4 mice per time point. Data are presented as mean and standard error of the mean (SEM). n.s., nonsignificant. P > 0.05, * P ≤0.05, ** P ≤0.01 by two-tailed unpaired t test.

    Article Snippet: After extensive washing, plates were incubated with biotin-labeled goat anti-mouse immunoglobulin G (IgG) and immunoglobulin M (IgM) (1 μg/ml, Southern Biotech, Birmingham, AL, USA) for 1 h and followed by ExtrAvidin™-alkaline phosphatase (Sigma-Aldrich) for 20 min.

    Techniques: Mouse Assay, Injection, Flow Cytometry, Cytometry, Generated, Expressing, Enzyme-linked Immunospot, Two Tailed Test

    T-oligo treatment does not cause apoptosis in normal primary B or T cells. ( a ) Primary B-2 cells were purified from mouse spleens, cultured and stimulated with 1 µg/ml goat anti-mouse IgM F(ab′) 2 antibody (μ chain specific), 1

    Journal: International journal of cancer. Journal international du cancer

    Article Title: Telomere homolog oligonucleotides induce apoptosis in malignant but not in normal lymphoid cells: Mechanism and therapeutic potential

    doi: 10.1002/ijc.23946

    Figure Lengend Snippet: T-oligo treatment does not cause apoptosis in normal primary B or T cells. ( a ) Primary B-2 cells were purified from mouse spleens, cultured and stimulated with 1 µg/ml goat anti-mouse IgM F(ab′) 2 antibody (μ chain specific), 1

    Article Snippet: Primary B cells were isolated from normal mouse spleens by anti-CD43 negative selection with magnetic beads (21; Miltenyi Biotec, Auburn, CA) and stimulated with anti-immunoglobulin M (IgM) (Jackson ImmunoResearch, West Grove, PA) and anti-CD40 antibodies (BD Pharmingen, San Diego, CA) and interleukin-4 (eBioscience, San Diego, CA).

    Techniques: Purification, Cell Culture

    The mean (±SD) serum IgG, IgM and IgA concentration of sows in different experimental groups; * – statistical difference compared to control group

    Journal: Journal of Veterinary Research

    Article Title: Effects of Newly Developed Synbiotic and Commercial Probiotic Products on The Haematological Indices, Serum Cytokines, Acute Phase Proteins Concentration, and Serum Immunoglobulins Amount in Sows and Growing Pigs – A Pilot Study

    doi: 10.2478/jvetres-2018-0046

    Figure Lengend Snippet: The mean (±SD) serum IgG, IgM and IgA concentration of sows in different experimental groups; * – statistical difference compared to control group

    Article Snippet: Measurement of serum immunoglobulins concentration Total serum concentrations of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were determined with a commercial ELISA kit (ELISA Quantitation Kit, Bethyl Laboratories Inc, USA), according to the manufacturer’s recommendations.

    Techniques: Concentration Assay

    The mean (±SD) serum IgG, IgM, and IgA concentration of growing pigs in different experimental groups; * – statistical difference compared to control group

    Journal: Journal of Veterinary Research

    Article Title: Effects of Newly Developed Synbiotic and Commercial Probiotic Products on The Haematological Indices, Serum Cytokines, Acute Phase Proteins Concentration, and Serum Immunoglobulins Amount in Sows and Growing Pigs – A Pilot Study

    doi: 10.2478/jvetres-2018-0046

    Figure Lengend Snippet: The mean (±SD) serum IgG, IgM, and IgA concentration of growing pigs in different experimental groups; * – statistical difference compared to control group

    Article Snippet: Measurement of serum immunoglobulins concentration Total serum concentrations of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were determined with a commercial ELISA kit (ELISA Quantitation Kit, Bethyl Laboratories Inc, USA), according to the manufacturer’s recommendations.

    Techniques: Concentration Assay

    PA SN induces lung injury after antibody-mediated platelet depletion of WT mice, and PA SN-induced injury can be attenuated by partial reconstitution of platelet counts in natively thrombocytopenic Mpl −/− mice. WT mice were administered anti-GP1bα or control antibody 24 hours prior to IT PA SN, and necropsy was performed at 20 hours with peripheral blood platelet counts (10 9 /L) (A). At necropsy 20 hours after IT PA SN inoculation, gross appearance of BAL fluid (B), BAL OD 540 measurements (C), BAL protein concentrations (mg/mL) (D), BAL IgM concentrations (ng/mL) (E), and BAL neutrophil counts/mL (F) are shown (n = 6 mice/group; 2 deaths occurred in the anti-GP1bα group prior to the 20-hour time point). In separate experiments, vehicle or 10 9 platelets from WT mice were transfused into Mpl −/− mice 1 hour following IT PA SN administration. Blood platelet counts (10 9 /L) (G), gross appearance of BAL fluid (H), and BAL OD 540 measurements (I) were obtained 20 hours post-PA infection. Each tube or point represents a single mouse (n = 5 for vehicle, n = 7 for the WT platelet group; peripheral blood was not successfully obtained from 1 mouse in the vehicle group), and the group median is displayed. Statistical comparison by Mann-Whitney U test. * P

    Journal: Blood Advances

    Article Title: Platelets inhibit apoptotic lung epithelial cell death and protect mice against infection-induced lung injury

    doi: 10.1182/bloodadvances.2018026286

    Figure Lengend Snippet: PA SN induces lung injury after antibody-mediated platelet depletion of WT mice, and PA SN-induced injury can be attenuated by partial reconstitution of platelet counts in natively thrombocytopenic Mpl −/− mice. WT mice were administered anti-GP1bα or control antibody 24 hours prior to IT PA SN, and necropsy was performed at 20 hours with peripheral blood platelet counts (10 9 /L) (A). At necropsy 20 hours after IT PA SN inoculation, gross appearance of BAL fluid (B), BAL OD 540 measurements (C), BAL protein concentrations (mg/mL) (D), BAL IgM concentrations (ng/mL) (E), and BAL neutrophil counts/mL (F) are shown (n = 6 mice/group; 2 deaths occurred in the anti-GP1bα group prior to the 20-hour time point). In separate experiments, vehicle or 10 9 platelets from WT mice were transfused into Mpl −/− mice 1 hour following IT PA SN administration. Blood platelet counts (10 9 /L) (G), gross appearance of BAL fluid (H), and BAL OD 540 measurements (I) were obtained 20 hours post-PA infection. Each tube or point represents a single mouse (n = 5 for vehicle, n = 7 for the WT platelet group; peripheral blood was not successfully obtained from 1 mouse in the vehicle group), and the group median is displayed. Statistical comparison by Mann-Whitney U test. * P

    Article Snippet: BAL immunoglobulin M (IgM) was determined following 1:10 dilution according to the manufacturer’s instructions (#E90-101, Bethyl Labs).

    Techniques: Mouse Assay, Infection, MANN-WHITNEY

    Reduction of neutrophil airspace influx does not significantly attenuate alveolar barrier disruption in thrombocytopenic Mpl −/− mice following either live PA challenge or PA SN-induced sterile injury. (A) Schematic of experimental design: Mpl −/− mice were administered Ly-6G or isotype control antibody (Ab) at −24 hours and inoculated with PA at 0 hours, and necropsy was performed 20 hours after PA lung infection. (B) Reduction of circulating neutrophil counts (10 9 /L blood) after anti-Ly6G. In separate experiments, mice were given either 10 6 live bacteria (C-G) or mice were given cell-free PA SN and a second dose of antibody at t = 0 hours (H-K). Following live bacterial challenge, lung CFU/mL (C), BAL neutrophil counts/mL (D), gross appearance of BAL fluid (E), BAL OD 540 (F), and BAL IgM concentrations (ng/mL) (G) were obtained at necropsy 20 hours after 10 6 CFU IT PA. n = 6 mice/group. There was 1 death in the isotype control group prior to the 20-hour time point. Following PA SN-induced sterile injury, BAL neutrophil counts/mL (H), gross appearance of BAL fluid (I), BAL OD 540 (J), and BAL IgM concentration (ng/mL) (K) were obtained at necropsy. n = 6 mice/group. There was 1 death in the isotype control group prior to the 20-hour time point. Each tube or point represents an individual mouse, and the group median is displayed. Statistical comparison by Mann-Whitney U test. ** P

    Journal: Blood Advances

    Article Title: Platelets inhibit apoptotic lung epithelial cell death and protect mice against infection-induced lung injury

    doi: 10.1182/bloodadvances.2018026286

    Figure Lengend Snippet: Reduction of neutrophil airspace influx does not significantly attenuate alveolar barrier disruption in thrombocytopenic Mpl −/− mice following either live PA challenge or PA SN-induced sterile injury. (A) Schematic of experimental design: Mpl −/− mice were administered Ly-6G or isotype control antibody (Ab) at −24 hours and inoculated with PA at 0 hours, and necropsy was performed 20 hours after PA lung infection. (B) Reduction of circulating neutrophil counts (10 9 /L blood) after anti-Ly6G. In separate experiments, mice were given either 10 6 live bacteria (C-G) or mice were given cell-free PA SN and a second dose of antibody at t = 0 hours (H-K). Following live bacterial challenge, lung CFU/mL (C), BAL neutrophil counts/mL (D), gross appearance of BAL fluid (E), BAL OD 540 (F), and BAL IgM concentrations (ng/mL) (G) were obtained at necropsy 20 hours after 10 6 CFU IT PA. n = 6 mice/group. There was 1 death in the isotype control group prior to the 20-hour time point. Following PA SN-induced sterile injury, BAL neutrophil counts/mL (H), gross appearance of BAL fluid (I), BAL OD 540 (J), and BAL IgM concentration (ng/mL) (K) were obtained at necropsy. n = 6 mice/group. There was 1 death in the isotype control group prior to the 20-hour time point. Each tube or point represents an individual mouse, and the group median is displayed. Statistical comparison by Mann-Whitney U test. ** P

    Article Snippet: BAL immunoglobulin M (IgM) was determined following 1:10 dilution according to the manufacturer’s instructions (#E90-101, Bethyl Labs).

    Techniques: Mouse Assay, Infection, Concentration Assay, MANN-WHITNEY

    Genetic deletion of PA T2SS (Δ xcpQ ), but not T3SS exotoxins (Δ exoTUY ), attenuates alveolar–capillary barrier disruption in thrombocytopenic Mpl −/− mice. Gross appearance of BAL fluid (A), BAL OD 540 measurements (B), BAL protein concentrations (mg/mL) (C), BAL IgM (ng/mL) (D), BAL neutrophil counts/mL (E), or lung MPO activity (U/left lung) (F) were obtained in Mpl −/− mice 20 hours following IT administration of vehicle or cell-free SN from PA14 or PA14Δ xcpQ strain (n = 5 mice in vehicle group, n = 8 mice in PA14 and PA14Δ xcpQ groups). In separate experiments, gross appearance of BAL fluid (G), BAL OD 540 measurements (H), and BAL protein concentrations (mg/mL) (I) were obtained in Mpl −/− mice 20 hours following IT administration of vehicle or cell-free SN from PA14 or PA14Δ exoTUY (n = 4 mice in vehicle group, n = 8 mice in PA14 and PA14Δ exoTUY groups). Each tube or point represents an individual mouse, and the group median is displayed. Statistical comparison by Kruskal-Wallis test with Dunn’s test for multiple comparisons (lines represent post hoc analysis). * P

    Journal: Blood Advances

    Article Title: Platelets inhibit apoptotic lung epithelial cell death and protect mice against infection-induced lung injury

    doi: 10.1182/bloodadvances.2018026286

    Figure Lengend Snippet: Genetic deletion of PA T2SS (Δ xcpQ ), but not T3SS exotoxins (Δ exoTUY ), attenuates alveolar–capillary barrier disruption in thrombocytopenic Mpl −/− mice. Gross appearance of BAL fluid (A), BAL OD 540 measurements (B), BAL protein concentrations (mg/mL) (C), BAL IgM (ng/mL) (D), BAL neutrophil counts/mL (E), or lung MPO activity (U/left lung) (F) were obtained in Mpl −/− mice 20 hours following IT administration of vehicle or cell-free SN from PA14 or PA14Δ xcpQ strain (n = 5 mice in vehicle group, n = 8 mice in PA14 and PA14Δ xcpQ groups). In separate experiments, gross appearance of BAL fluid (G), BAL OD 540 measurements (H), and BAL protein concentrations (mg/mL) (I) were obtained in Mpl −/− mice 20 hours following IT administration of vehicle or cell-free SN from PA14 or PA14Δ exoTUY (n = 4 mice in vehicle group, n = 8 mice in PA14 and PA14Δ exoTUY groups). Each tube or point represents an individual mouse, and the group median is displayed. Statistical comparison by Kruskal-Wallis test with Dunn’s test for multiple comparisons (lines represent post hoc analysis). * P

    Article Snippet: BAL immunoglobulin M (IgM) was determined following 1:10 dilution according to the manufacturer’s instructions (#E90-101, Bethyl Labs).

    Techniques: Mouse Assay, Activity Assay

    Trend toward improved transalveolar-capillary membrane protein transit, as evidenced by BALF levels of IgM and albumin in the VPA-treated mice. (* p

    Journal: The journal of trauma and acute care surgery

    Article Title: Valproic acid mitigates the inflammatory response and prevents acute respiratory distress syndrome in a murine model of Escherichia coli pneumonia at the expense of bacterial clearance

    doi: 10.1097/TA.0000000000001389

    Figure Lengend Snippet: Trend toward improved transalveolar-capillary membrane protein transit, as evidenced by BALF levels of IgM and albumin in the VPA-treated mice. (* p

    Article Snippet: Albumin and Immunoglobulin M (IgM) levels were assessed in the BALF by ELISA (Bethyl Laboratories, Montgomery, TX) to assess degree of transalveolar-capillary membrane protein transit, a marker of alveolar wall destruction and alveolar-capillary membrane permeability.

    Techniques: Mouse Assay

    AQP4-negative GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and IgM-positive (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).

    Journal: Journal of Neuropathology and Experimental Neurology

    Article Title: Multiple Sclerosis: Destruction and Regeneration of Astrocytes in Acute Lesions

    doi: 10.1093/jnen/nly121

    Figure Lengend Snippet: AQP4-negative GFAP-positive astrocytes. Skip serial sections of an inactive late postphagocytic plaque (no LFB-positive or MBP-positive macrophages) in a patient with fulminant early MS (Case 3). (A, B) None of the numerous GFAP-positive stellate astrocytes present in the plaque is reactive for AQP4. (C, D) IgG-positive (C) and IgM-positive (D) astrocytes are indicative of an open blood-brain barrier. (E, F) MH2-positive inflammatory cells and macrophages are restricted largely to the perivascular cuff (E) . This, and the paucity of MRP-positive monocytes (F) , indicate a waning inflammatory milieu. (An inactive plaque in Case 3, disease duration 29 days). (A : GFAP; B : AQP4; C : IgG; D : IgM; E : MHC2; F : MRP14. A–F : ×115 ).

    Article Snippet: Primary antibodies comprised GFAP (clone GF12-24, Chemicon, Boronia, Australia), GFAP polyclonal (DakoCytomation, Carpinteria, CA), GFAPδ polyclonal (EMD Millipore Corp, Temecula, CA), AQP4 polyclonal (Millipore Corp, Billerica, MA), S-100 polyclonal (Abcam, Cambridge, UK), IgM (DakoCytomation), CD45RO (clone UCHL1, Novocastra, Newcastle-Upon-Tyne, UK).

    Techniques: Mass Spectrometry

    Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific immunoglobulin M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; IgM, immunoglobulin M; TCID 50 , 50% tissue culture infective dose.

    Journal: The Journal of Infectious Diseases

    Article Title: A Case of Human Lassa Virus Infection With Robust Acute T-Cell Activation and Long-Term Virus-Specific T-Cell Responses

    doi: 10.1093/infdis/jix201

    Figure Lengend Snippet: Class switching corresponds with peak frequency of antibody-secreting cells. A, Patient samples were analyzed by enzyme-linked immunosorbent assay for Lassa virus–specific immunoglobulin M and immunoglobulin G responses, and viral loads were determined by 50% tissue culture infective dose assay. The hash-marked line represents the limit of detection of the viral load assay. B, Representative flow plots for both antibody-secreting cells (ASCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20loCD71 + ) and activated B cells (ABCs; CD3 − CD14 − CD16 − IgD − CD19 + CD20 ++ CD71 + ) are depicted for both the patient (at day 23) and a healthy control, and frequencies of ASCs and ABCs were assessed over time. Abbreviations: ABC, activated B cell; ASC, antibody-secreting cell; IgG, immunoglobulin G; IgM, immunoglobulin M; TCID 50 , 50% tissue culture infective dose.

    Article Snippet: After 3 washes in PBST, plates were incubated for 1 hour at 37°C with antihuman IgG HRP (horseradish peroxidase) or antihuman immunoglobulin M (IgM) HRP (Jackson ImmunoResearch Inc) diluted 1:5000 in blocking solution.

    Techniques: Enzyme-linked Immunosorbent Assay, Viral-load Assay, Flow Cytometry

    GDC-0853 represses NF-κB–dependent gene transcription. CLL patient cells were treated with 1 µM BTK inhibitor for 72 hours with or without anti-IgM stimulation and assessed for gene expression changes of NF-κB targets by RT-PCR (N = 8). ** P ≤ .01; *** P ≤ .001.

    Journal: Blood

    Article Title: Noncovalent inhibition of C481S Bruton tyrosine kinase by GDC-0853: a new treatment strategy for ibrutinib-resistant CLL

    doi: 10.1182/blood-2017-10-809020

    Figure Lengend Snippet: GDC-0853 represses NF-κB–dependent gene transcription. CLL patient cells were treated with 1 µM BTK inhibitor for 72 hours with or without anti-IgM stimulation and assessed for gene expression changes of NF-κB targets by RT-PCR (N = 8). ** P ≤ .01; *** P ≤ .001.

    Article Snippet: CLL cells treated with GDC-0853 or ibrutinib were stimulated through their BCR by spinning onto a 6-well plate coated with 10 µg/mL anti-immunoglobulin M (anti-IgM) antibody (Jackson Laboratories).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Antibody dynamics of Salmonella enterica serovar Typhimurium–specific antibodies (ie, O4) and S . enterica serovar Enteritidis–specific antibodies (ie, O9) during the first 12 months of life in infants from Ho Chi Minh City, Vietnam. Plots show a generalized additive mixed-effect model–fitted population trend (solid black line) for anti-O4 immunoglobulin M (IgM; A ), anti-O4 immunoglobulin G (IgG; B ), anti-O9 IgM ( C ), and anti-O9 IgG ( D ) from birth through 12 months of age. 95% confidence intervals are shown by the dashed black lines.

    Journal: The Journal of Infectious Diseases

    Article Title: The Role of Maternally Acquired Antibody in Providing Protective Immunity Against Nontyphoidal Salmonella in Urban Vietnamese Infants: A Birth Cohort Study

    doi: 10.1093/infdis/jiy501

    Figure Lengend Snippet: Antibody dynamics of Salmonella enterica serovar Typhimurium–specific antibodies (ie, O4) and S . enterica serovar Enteritidis–specific antibodies (ie, O9) during the first 12 months of life in infants from Ho Chi Minh City, Vietnam. Plots show a generalized additive mixed-effect model–fitted population trend (solid black line) for anti-O4 immunoglobulin M (IgM; A ), anti-O4 immunoglobulin G (IgG; B ), anti-O9 IgM ( C ), and anti-O9 IgG ( D ) from birth through 12 months of age. 95% confidence intervals are shown by the dashed black lines.

    Article Snippet: LPS-binding antibodies were detected using alkaline phosphatase-conjugated anti-human IgG and immunoglobulin M (IgM) secondary antibodies (for 1 hour at ambient room temperature), and developed using p-nitrophenyl-phosphate solution (SigmaFAST N1891; Sigma-Aldrich, UK), with absorbance measured at 405 and 490nm.

    Techniques:

    (a) B-cell proliferation upon stimulation with CpG. Data representative of at least three different experiments. (b) Antibody production after in vitro CpG stimulation of peripheral blood mononuclear cells. Antibodies of IgM, IgA and IgG isotype were

    Journal:

    Article Title: A novel immunodeficiency characterized by the exclusive presence of transitional B cells unresponsive to CpG

    doi: 10.1111/j.1365-2567.2006.02556.x

    Figure Lengend Snippet: (a) B-cell proliferation upon stimulation with CpG. Data representative of at least three different experiments. (b) Antibody production after in vitro CpG stimulation of peripheral blood mononuclear cells. Antibodies of IgM, IgA and IgG isotype were

    Article Snippet: Briefly, 96-well plates (Corning Incorporated, New York, NY) were coated overnight with purified goat anti-human IgA, IgG or IgM (Jackson ImmunoResearch).

    Techniques: In Vitro

    Binding of serum immunoglobulins and C192S. Human sera were passed through an affinity column immobilized with C192S, and then the binding ligands of C192S were eluted and the fraction 13 (F13) was collected. The contents in diluted human serum, different amount of purified human immunoglobulins, and F13 (1 µg) were verified using 12% SDS-PAGE (A) and blotted with goat anti-human IgG (B), IgM (C), or IgA (D). (E) 2 µM of purified C192S or BSA was coated in a 96-well ELISA plate, and binding of IgG, IgM, or IgA with C192S or BSA was detected by ELISA, as described in Materials and Methods. ** P

    Journal: PLoS ONE

    Article Title: Determining Antibody-Binding Site of Streptococcal Pyrogenic Exotoxin B to Protect Mice from Group A Streptococcus Infection

    doi: 10.1371/journal.pone.0055028

    Figure Lengend Snippet: Binding of serum immunoglobulins and C192S. Human sera were passed through an affinity column immobilized with C192S, and then the binding ligands of C192S were eluted and the fraction 13 (F13) was collected. The contents in diluted human serum, different amount of purified human immunoglobulins, and F13 (1 µg) were verified using 12% SDS-PAGE (A) and blotted with goat anti-human IgG (B), IgM (C), or IgA (D). (E) 2 µM of purified C192S or BSA was coated in a 96-well ELISA plate, and binding of IgG, IgM, or IgA with C192S or BSA was detected by ELISA, as described in Materials and Methods. ** P

    Article Snippet: The reaction mixtures mentioned above were separated using 12% SDS-PAGE and then detected by western blot with goat anti-human IgG (Calbiochem), IgM (Abcam), or IgA antibody (Abcam).

    Techniques: Binding Assay, Affinity Column, Purification, SDS Page, Enzyme-linked Immunosorbent Assay

    Effect of SPE B on immunoglobulins. (A) Either 400 µg/ml of purified IgG or the IgM-IgA mixture was incubated with 20 µg/ml of mSPE B or C192S for 1 h with 5 mM DTT-0.1 mM EDTA. The reaction mixture was separated using 12% SDS-PAGE and blotted using goat anti-human IgG, IgM, or IgA, as described in Materials and Methods. (B) Either 400 µg/ml of purified IgG or the IgM-IgA mixture was incubated with 20 µg/ml of mSPE B for 1, 2 or 18 h with 5 mM DTT-0.1 mM EDTA. The reaction mixture was separated using 12% SDS-PAGE and blotted using goat anti-human IgG, IgM, or IgA, as described in Materials and Methods.

    Journal: PLoS ONE

    Article Title: Determining Antibody-Binding Site of Streptococcal Pyrogenic Exotoxin B to Protect Mice from Group A Streptococcus Infection

    doi: 10.1371/journal.pone.0055028

    Figure Lengend Snippet: Effect of SPE B on immunoglobulins. (A) Either 400 µg/ml of purified IgG or the IgM-IgA mixture was incubated with 20 µg/ml of mSPE B or C192S for 1 h with 5 mM DTT-0.1 mM EDTA. The reaction mixture was separated using 12% SDS-PAGE and blotted using goat anti-human IgG, IgM, or IgA, as described in Materials and Methods. (B) Either 400 µg/ml of purified IgG or the IgM-IgA mixture was incubated with 20 µg/ml of mSPE B for 1, 2 or 18 h with 5 mM DTT-0.1 mM EDTA. The reaction mixture was separated using 12% SDS-PAGE and blotted using goat anti-human IgG, IgM, or IgA, as described in Materials and Methods.

    Article Snippet: The reaction mixtures mentioned above were separated using 12% SDS-PAGE and then detected by western blot with goat anti-human IgG (Calbiochem), IgM (Abcam), or IgA antibody (Abcam).

    Techniques: Purification, Incubation, SDS Page

    Binding of SPE B truncations and immunoglobulins. Either purified human IgG (A) or the IgM-IgA mixture (B) was coated in a 96-well ELISA plate, and binding of different SPE B truncations with IgG or the IgM-IgA mixture was detected by ELISA, as described in Materials and Methods. * P

    Journal: PLoS ONE

    Article Title: Determining Antibody-Binding Site of Streptococcal Pyrogenic Exotoxin B to Protect Mice from Group A Streptococcus Infection

    doi: 10.1371/journal.pone.0055028

    Figure Lengend Snippet: Binding of SPE B truncations and immunoglobulins. Either purified human IgG (A) or the IgM-IgA mixture (B) was coated in a 96-well ELISA plate, and binding of different SPE B truncations with IgG or the IgM-IgA mixture was detected by ELISA, as described in Materials and Methods. * P

    Article Snippet: The reaction mixtures mentioned above were separated using 12% SDS-PAGE and then detected by western blot with goat anti-human IgG (Calbiochem), IgM (Abcam), or IgA antibody (Abcam).

    Techniques: Binding Assay, Purification, Enzyme-linked Immunosorbent Assay

    rSPE B 345–398 interfered with SPE B cleaving immunoglobulins. Either 400 µg/ml of purified human IgG or the IgM-IgA mixture was incubated with 20 µg/ml of mSPE B in the absence or presence of different concentrations of rSPE B 345–398 at 37°C for 1 h with 5 mM DTT-0.1 mM EDTA. The reaction mixtures were separated using 12% SDS-PAGE and then detected by western blotting with goat anti-human IgG, IgM, or IgA antibodies. Lane 1, purified immunoglobulins; lane 2, mSPE B-treated immunoglobulins; lane 3–5, SPE B-treated immunoglobulins incubated with different concentrations of rSPE B 345–398 ; in lane 3, 4, and 5 the molar ratio of rSPE B 345–398 /mSPE B was 5, 10, and 20, respectively.

    Journal: PLoS ONE

    Article Title: Determining Antibody-Binding Site of Streptococcal Pyrogenic Exotoxin B to Protect Mice from Group A Streptococcus Infection

    doi: 10.1371/journal.pone.0055028

    Figure Lengend Snippet: rSPE B 345–398 interfered with SPE B cleaving immunoglobulins. Either 400 µg/ml of purified human IgG or the IgM-IgA mixture was incubated with 20 µg/ml of mSPE B in the absence or presence of different concentrations of rSPE B 345–398 at 37°C for 1 h with 5 mM DTT-0.1 mM EDTA. The reaction mixtures were separated using 12% SDS-PAGE and then detected by western blotting with goat anti-human IgG, IgM, or IgA antibodies. Lane 1, purified immunoglobulins; lane 2, mSPE B-treated immunoglobulins; lane 3–5, SPE B-treated immunoglobulins incubated with different concentrations of rSPE B 345–398 ; in lane 3, 4, and 5 the molar ratio of rSPE B 345–398 /mSPE B was 5, 10, and 20, respectively.

    Article Snippet: The reaction mixtures mentioned above were separated using 12% SDS-PAGE and then detected by western blot with goat anti-human IgG (Calbiochem), IgM (Abcam), or IgA antibody (Abcam).

    Techniques: Purification, Incubation, SDS Page, Western Blot

    Assessment of the nonimmune Ig-binding properties of 3D7 VAR2CSA domains by FACS. ( A ) Domain structure of 3D7 VAR2CSA. IgG- and IgM-binding domains as well as previously identified CSA-binding domains are indicated. ( B ) Data shown are flow-cytometric

    Journal:

    Article Title: Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin

    doi: 10.1073/pnas.0601519103

    Figure Lengend Snippet: Assessment of the nonimmune Ig-binding properties of 3D7 VAR2CSA domains by FACS. ( A ) Domain structure of 3D7 VAR2CSA. IgG- and IgM-binding domains as well as previously identified CSA-binding domains are indicated. ( B ) Data shown are flow-cytometric

    Article Snippet: Each parasite line was tested with the following reagents: FITC-conjugated sheep anti-human Ig (final concentration 40 μg/ml; SBL Vaccines, Stockholm, Sweden) , mouse mAbs to human IgG-Fc (clone MK1A6; Serotec, Hornby, ON, Canada), IgM (clone M15/8; Serotec), and matched isotype control (clone W3/25; Serotec).

    Techniques: Binding Assay, FACS, Flow Cytometry

    Nonimmune IgG- and IgM-binding profile of fresh Ugandan placental isolates ( n = 22) assessed by live SIFA. Each data point represents the Ig-, IgG-, or IgM-binding level of one placental isolate. Each placental isolate was tested directly upon elution

    Journal:

    Article Title: Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin

    doi: 10.1073/pnas.0601519103

    Figure Lengend Snippet: Nonimmune IgG- and IgM-binding profile of fresh Ugandan placental isolates ( n = 22) assessed by live SIFA. Each data point represents the Ig-, IgG-, or IgM-binding level of one placental isolate. Each placental isolate was tested directly upon elution

    Article Snippet: Each parasite line was tested with the following reagents: FITC-conjugated sheep anti-human Ig (final concentration 40 μg/ml; SBL Vaccines, Stockholm, Sweden) , mouse mAbs to human IgG-Fc (clone MK1A6; Serotec, Hornby, ON, Canada), IgM (clone M15/8; Serotec), and matched isotype control (clone W3/25; Serotec).

    Techniques: Binding Assay

    Ig-binding profile of fresh placental isolates. Correlation between data obtained with two different assays, ex vivo placental adhesion/inhibition (IgG) and live SIFA (Ig, IgM, and IgG).

    Journal:

    Article Title: Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin

    doi: 10.1073/pnas.0601519103

    Figure Lengend Snippet: Ig-binding profile of fresh placental isolates. Correlation between data obtained with two different assays, ex vivo placental adhesion/inhibition (IgG) and live SIFA (Ig, IgM, and IgG).

    Article Snippet: Each parasite line was tested with the following reagents: FITC-conjugated sheep anti-human Ig (final concentration 40 μg/ml; SBL Vaccines, Stockholm, Sweden) , mouse mAbs to human IgG-Fc (clone MK1A6; Serotec, Hornby, ON, Canada), IgM (clone M15/8; Serotec), and matched isotype control (clone W3/25; Serotec).

    Techniques: Binding Assay, Ex Vivo, Inhibition