iglon2 Search Results


iglon2  (OriGene)
93
OriGene iglon2
Membrane proteins identified as bona-fide IgLON5 interactors.
Iglon2, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
iglon2 - by Bioz Stars, 2026-03
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iglon2  (Proteintech)
92
Proteintech iglon2
FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row <t>IgLON2</t> (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.
Iglon2, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iglon2/product/Proteintech
Average 92 stars, based on 1 article reviews
iglon2 - by Bioz Stars, 2026-03
92/100 stars
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Membrane proteins identified as bona-fide IgLON5 interactors.

Journal: Frontiers in Immunology

Article Title: Patients’ IgLON5 autoantibodies interfere with IgLON5-protein interactions

doi: 10.3389/fimmu.2023.1151574

Figure Lengend Snippet: Membrane proteins identified as bona-fide IgLON5 interactors.

Article Snippet: To validate cis -interactions (within the same cell) of IgLON5 with identified interactors, HEK cells were co-transfected in pairs: IGLON5 (SC317071, Origene) plus: 1) IGLON1 (RG213594, Origene), 2) IGLON2 (RG226879, Origene), 3) IGLON3 (RG207618, Origene), 4) IGLON4 (RG216034 Origene), 5) KIDINS220 (RC216441Origene), 6) IGSF21 (RG203559, Origene), 7) CACNA2D2 (RC219452, Origene) and 8) CASPR2 (SC115167, Origene), as a non-binding control.

Techniques: Membrane

Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Journal: Frontiers in Immunology

Article Title: Patients’ IgLON5 autoantibodies interfere with IgLON5-protein interactions

doi: 10.3389/fimmu.2023.1151574

Figure Lengend Snippet: Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Article Snippet: To validate cis -interactions (within the same cell) of IgLON5 with identified interactors, HEK cells were co-transfected in pairs: IGLON5 (SC317071, Origene) plus: 1) IGLON1 (RG213594, Origene), 2) IGLON2 (RG226879, Origene), 3) IGLON3 (RG207618, Origene), 4) IGLON4 (RG216034 Origene), 5) KIDINS220 (RC216441Origene), 6) IGSF21 (RG203559, Origene), 7) CACNA2D2 (RC219452, Origene) and 8) CASPR2 (SC115167, Origene), as a non-binding control.

Techniques: Western Blot, Immunoprecipitation, Transfection, Incubation

FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Journal: Frontiers in immunology

Article Title: Patients' IgLON5 autoantibodies interfere with IgLON5-protein interactions.

doi: 10.3389/fimmu.2023.1151574

Figure Lengend Snippet: FIGURE 2 Immunoblots of co-tranfected HEK cells immunoprecipitated with patients’ IgLON5 antibodies. HEK cells co-transfected with IgLON5 and each of the other IgLON family members: first row (IgLON5 + IgLON1), 2nd row IgLON2 (5+2), 3rd row (5+3), and 4th row (5+4). The 5th row corresponds to HEK cells transfected only with IgLON5, and the 6th row HEK cells co-transfected with IgLON5 and CASPR2 (5+C). Left column: Whole cell lysates (WCL); middle column: Immunoprecipitates of transfected HEK cells using patients’ IgLON5-abs (IP+); right column: Immunoprecipitates of transfected cells using Ctrl-abs (IP). Each row was incubated with commercial antibodies against the corresponding proteins: IgLON1, 2,3,4,5 and CASPR2.

Article Snippet: The presence of the interacting proteins was detected by incubating the membrane with the following antibodies (dilution 1:500, overnight at 4°C): IgLON1 (STJ94590), IgLON2 (STJ94442), IgLON3 (STJ116461), IgLON4 (STJ94394, St. John’s Laboratory, London, UK), KIDINS220 (21856-1-AP, Proteintech, Rosemont, IL, USA), IGSF21 (21465-1-AP; Proteintech), CACNA2D2 (SAB1401461, SIGMA) and CASPR2 (ab33994, Abcam) followed by incubation with appropriate secondary antibodies (dilution 1:1000) for 1 hour at room temperature.

Techniques: Western Blot, Immunoprecipitation, Transfection, Incubation