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  • 99
    Thermo Fisher cd4 igg2
    Cell-surface expression of wild-type and gp41 mutant Env glycoproteins and their reactivity with <t>CD4-IgG2</t> and MAbs. (A) Cell surface expressed Envs were biotinylated, avidin-precipitated and detected using MAb ARP3119. Cell surface expressed CD47 served as a loading control (lower panel). (B) The WT and gp41 mutant Env glycoproteins were stained with 10 µg/ml of biotinylated MAbs, followed by streptavidin-PE. Background fluorescence due to the secondary antibody was determined using isotype controls and subtracted from experimental values. The MFI values are shown as Mean ± Standard Deviation from a representative experiment performed in triplicates.
    Cd4 Igg2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore igg2
    Evaluation of plasma binding to WT and mutant trimers by BN-PAGE. Plasmas were titrated against WT, D368R, or N295A trimers in BN-PAGE as indicated. Cartoons depict gp120/gp41 trimers, <t>trimer-IgG</t> complexes, and monomers. Markers show the locations of ferritin markers of 220 and 439 kDa and trimers complexed with one, two, or three molecules of IgG 2G12 (molecular masses of ∼570, ∼720, and ∼870 kDa). Arrows indicate the dilution corresponding to the ID 50 titer, inferred from at least three duplicate titrations. The unbound fraction of plasma BB34 absorbed against WT gp120 beads was also evaluated for plasma BB34, identified as “F/TH,” flowthrough. (−), blank beads.
    Igg2, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher igg2
    <t>IgG</t> subclass specificity of gingipain K. A ) Human IgG subclasses 1–4 were incubated with gingipain K for 4 h at 37°C and visualized by SDS-PAGE under reducing conditions. From densitometric scanning, molecular masses of IgG heavy and light
    Igg2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 441 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad igg2
    ( A – H ) High-avidity PRRSV specific and virus-neutralizing (VN) antibodies were produced in pigs vaccinated with adjuvanted NP-KAg. Pigs were vaccinated or unvaccinated with indicated vaccine and adjuvant combination and challenged with PRRSV MN184. The plasma samples collected from pigs of 100 μg per pig vaccine dose ( A – C ) or 500 μg dose category ( E – G ) on the day of challenge (PC 0), followed by PC 6 and PC 15 (day of necropsy), were analyzed for avidity of PRRSV-specific <t>IgG</t> by ELISA. Each symbol indicates the mean of percentage absorbance compared to no ammonium thiocyanate (0 M) ± SEM of three pigs. The VN antibody titers in the plasma samples of 100 μg per pig vaccine dose ( D ) or 500 μg dose category ( H ) were analyzed by indirect immunofluorescence assay. Each symbol in the line graph indicates the average VN titer of three pigs ± SEM at indicated time point of blood collection. Lowercase letters indicate statistically significant ( P
    Igg2, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson igg2
    Anti- S. aureus IgG1 and <t>IgG2</t> titers in paired maternal and cord serum samples from the case and control groups. (a) Anti- S. aureus IgG1 titers and (d) anti- S. aureus IgG2 titers in the maternal serum and umbilical cord serum samples from the case (n=48) and control group (n=98). In (b) and (c) , correlation coefficients between paired maternal and umbilical cord serum of anti- Sa IgG1 in the case and control group, respectively. In (e) and (f) , correlation coefficients between the paired maternal and umbilical cord serum of anti- Sa IgG2 in the case and control group, respectively. In (a) and (d) , the box represents the 25 th –75 th percentiles, and the median is represented by the line within the box. The whiskers represent the 5 th –95 th percentiles.
    Igg2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Progenics cd4 igg2
    Antigenic characterization of Env IL-4 and Env IL-21 molecules. ELISA reactivity of Env IL-4 and Env IL-21 with 2G12 and HIV-Ig (A); b12 and <t>CD4-IgG2</t> (B); and 48d (CD4i) in the absence and presence of sCD4 at 1 µg/ml (C). All ELISA results are representative for at least three independent experiments using proteins derived from three independent transfections.
    Cd4 Igg2, supplied by Progenics, used in various techniques. Bioz Stars score: 89/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Amgen igg2 monoclonal antibody
    Antigenic characterization of Env IL-4 and Env IL-21 molecules. ELISA reactivity of Env IL-4 and Env IL-21 with 2G12 and HIV-Ig (A); b12 and <t>CD4-IgG2</t> (B); and 48d (CD4i) in the absence and presence of sCD4 at 1 µg/ml (C). All ELISA results are representative for at least three independent experiments using proteins derived from three independent transfections.
    Igg2 Monoclonal Antibody, supplied by Amgen, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    SouthernBiotech mouse anti human igg
    Comparison of the levels of the apoptotic marker CC3 (A,C) and the proliferation marker Ki67 (B,D) in the tumors of the mice passively immunized with the rabbit <t>IgG</t> against the <t>mPD1-derived</t> mimotope, or in the mice actively immunized with the mimotope, evaluated by IHC staining. For each tumor, more than one region was quantified for detection of evaluated markers. Representative images are shown. Significant differences are indicated by asterisks (* P
    Mouse Anti Human Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    igg2  (Abcam)
    92
    Abcam igg2
    Anti-rhGAA IgG1 and IgG4 are the most prevalent subclasses of antibodies developed in response to long-term ERT. ( A – F ) Anti-rhGAA antibodies measured with a capture assay specific for IgG1, <t>IgG2,</t> IgG3, IgG4, IgM, and IgE antibodies. Estimated antibody concentrations are reported for each subject. Average and standard deviation are indicated for each of the study cohorts: LOPD subjects receiving ERT (Treated, n = 28), untreated LOPD subjects (Untreated, n = 10), and healthy donors (HD, n = 43). Unpaired two-tailed t-test was used to compare results across the study cohorts (**P
    Igg2, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Pharmingen igg2
    Anti-rhGAA IgG1 and IgG4 are the most prevalent subclasses of antibodies developed in response to long-term ERT. ( A – F ) Anti-rhGAA antibodies measured with a capture assay specific for IgG1, <t>IgG2,</t> IgG3, IgG4, IgM, and IgE antibodies. Estimated antibody concentrations are reported for each subject. Average and standard deviation are indicated for each of the study cohorts: LOPD subjects receiving ERT (Treated, n = 28), untreated LOPD subjects (Untreated, n = 10), and healthy donors (HD, n = 43). Unpaired two-tailed t-test was used to compare results across the study cohorts (**P
    Igg2, supplied by Pharmingen, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad mouse anti bovine igg1
    Profiles of the FMDV-ASC detected in B 2 T- and B 4 T-vaccinated cattle after FMDV challenge. (a) Mononuclear cells were purified from mandibular lymph nodes (ML), medial retropharyngeal lymph nodes (MRL), and tracheobronchial lymph nodes (TBL) and characterized by the FMDV-ASC ELISPOT assay, using monoclonal <t>(IgG1</t> and <t>IgG2)</t> or polyclonal (IgM and IgA) antibodies against bovine immunoglobulin isotypes as probes. (b) Total FMDV-ASC in ML, MRL, or TBL. Results are expressed as the mean number of FMDV-specific ASC per 1 × 10 6 extracted cells. Each bar represents the mean value of 3 replicates ± SD. PPG: protected against podal generalization; PP: partial protected; NP: non-protected.
    Mouse Anti Bovine Igg1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore monoclonal anti human igg3 biotin antibody
    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum <t>IgG</t> ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.
    Monoclonal Anti Human Igg3 Biotin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bethyl sheep anti dog igg2
    Dogs immunized with the Lu. longipalpis salivary molecules LJM17 or LJL143 develop strong and specific humoral and cellular immune responses. (A) Total <t>IgG</t> and (B) IgG 1 and IgG 2 antibody levels up to day 224 in dogs immunized with either LJM17 (n = 5), LJL143 (n = 5) or the empty plasmid (n = 5). LJL143- and LJM17-immunized dogs were tested using the appropriate recombinant proteins (LJL143 and LJM17). Dogs immunized with the empty vector (control dogs) were tested against both recombinant proteins for IgG, (LJL143-control, LJM17-control), IgG 1 (IgG 1 -control) and IgG 2 (IgG 2 -control). (C) In vitro IFN-γ production by PBMC from LJL143- and LJM17-immunized and control dogs stimulated with media (Med), ConcavalinA (ConA), salivary gland homogenate (SGH), rLJM17 or rLJL143 two weeks after the final vaccination. Error bars represent means±S.E.
    Sheep Anti Dog Igg2, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore anti igg2
    Enzyme-linked immunosorbent assay (ELISA) anti-peptide <t>IgG</t> titers upon vaccination with a mixture of N, R, and C peptides. Panels show responses to mixtures using 150 μg total peptide dose in ( Row A ) Montanide ISA 720 or ( Row B ) Montanide ISA 51 and to mixtures using 300 μg total peptide dose in ( Row C ) Montanide ISA 720 or ( Row D ) Montanide ISA 51. Immunizations were made in the 0, 2, and 4 months.
    Anti Igg2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Becton Dickinson biotinylated anti syrian hamster igg2
    Enzyme-linked immunosorbent assay (ELISA) anti-peptide <t>IgG</t> titers upon vaccination with a mixture of N, R, and C peptides. Panels show responses to mixtures using 150 μg total peptide dose in ( Row A ) Montanide ISA 720 or ( Row B ) Montanide ISA 51 and to mixtures using 300 μg total peptide dose in ( Row C ) Montanide ISA 720 or ( Row D ) Montanide ISA 51. Immunizations were made in the 0, 2, and 4 months.
    Biotinylated Anti Syrian Hamster Igg2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore human igg2
    Isolation of Nm proteins binding to human <t>IgG</t> by precipitation with Protein A or G beads. NmW135 strain A22 was cultured in CDM+HuS or MH as indicated. Unmarked lanes were intentionally left unloaded. The arrows indicate bands that produced a common set
    Human Igg2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Prionics Lelystad B.V igg2
    Antibody responses against LukM. IgG1 ( a , b , c ) and <t>IgG2</t> ( d , e , f ) antibodies specific for LukM measured in serum ( a , d ), colostrum ( b , e ) and milk ( c , f ). S/P = Sample to positive ratio. + = P
    Igg2, supplied by Prionics Lelystad B.V, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson hamster igg2
    Antibody responses against LukM. IgG1 ( a , b , c ) and <t>IgG2</t> ( d , e , f ) antibodies specific for LukM measured in serum ( a , d ), colostrum ( b , e ) and milk ( c , f ). S/P = Sample to positive ratio. + = P
    Hamster Igg2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore igg2 antibodies
    Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies <t>(IgG</t> total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) <t>IgG2a:IgG1</t> ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and α-MEM medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p
    Igg2 Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Athens Research igg2
    <t>IgG</t> binding characteristics of FcγR1
    Igg2, supplied by Athens Research, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Progenics cd4 igg2 fusion protein
    sCD4 and <t>CD4-IgG2</t> induce the shedding of Bx08 gp120. Purified virus was incubated with PBS (mock-treated control, left panel) or 50-μg/ml sCD4 (central panel) or CD4-IgG2 (right panel) for 1 h at 37°C, and viral particles were further purified by parallel gel filtration columns. Dosage determinations for the viral proteins p24 (empty squares) and gp120 (black triangles) in the collected fractions were performed as indicated in Materials and Methods.
    Cd4 Igg2 Fusion Protein, supplied by Progenics, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Pfizer Inc human igg2
    sCD4 and <t>CD4-IgG2</t> induce the shedding of Bx08 gp120. Purified virus was incubated with PBS (mock-treated control, left panel) or 50-μg/ml sCD4 (central panel) or CD4-IgG2 (right panel) for 1 h at 37°C, and viral particles were further purified by parallel gel filtration columns. Dosage determinations for the viral proteins p24 (empty squares) and gp120 (black triangles) in the collected fractions were performed as indicated in Materials and Methods.
    Human Igg2, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Bio-Rad sheep anti bovine igg1 hrp
    sCD4 and <t>CD4-IgG2</t> induce the shedding of Bx08 gp120. Purified virus was incubated with PBS (mock-treated control, left panel) or 50-μg/ml sCD4 (central panel) or CD4-IgG2 (right panel) for 1 h at 37°C, and viral particles were further purified by parallel gel filtration columns. Dosage determinations for the viral proteins p24 (empty squares) and gp120 (black triangles) in the collected fractions were performed as indicated in Materials and Methods.
    Sheep Anti Bovine Igg1 Hrp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 87/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cell-surface expression of wild-type and gp41 mutant Env glycoproteins and their reactivity with CD4-IgG2 and MAbs. (A) Cell surface expressed Envs were biotinylated, avidin-precipitated and detected using MAb ARP3119. Cell surface expressed CD47 served as a loading control (lower panel). (B) The WT and gp41 mutant Env glycoproteins were stained with 10 µg/ml of biotinylated MAbs, followed by streptavidin-PE. Background fluorescence due to the secondary antibody was determined using isotype controls and subtracted from experimental values. The MFI values are shown as Mean ± Standard Deviation from a representative experiment performed in triplicates.

    Journal: Virology

    Article Title: N-terminal substitutions in HIV-1 gp41 reduce the expression of non-trimeric envelope glycoproteins on the virus

    doi: 10.1016/j.virol.2007.10.018

    Figure Lengend Snippet: Cell-surface expression of wild-type and gp41 mutant Env glycoproteins and their reactivity with CD4-IgG2 and MAbs. (A) Cell surface expressed Envs were biotinylated, avidin-precipitated and detected using MAb ARP3119. Cell surface expressed CD47 served as a loading control (lower panel). (B) The WT and gp41 mutant Env glycoproteins were stained with 10 µg/ml of biotinylated MAbs, followed by streptavidin-PE. Background fluorescence due to the secondary antibody was determined using isotype controls and subtracted from experimental values. The MFI values are shown as Mean ± Standard Deviation from a representative experiment performed in triplicates.

    Article Snippet: CD4-IgG2 and MAbs were biotinylated using the EZ-link Sulfo-NHS-LC-Biotinylation kit (Pierce), according to the manufacturer’s instructions.

    Techniques: Expressing, Mutagenesis, Avidin-Biotin Assay, Staining, Fluorescence, Standard Deviation

    Evaluation of plasma binding to WT and mutant trimers by BN-PAGE. Plasmas were titrated against WT, D368R, or N295A trimers in BN-PAGE as indicated. Cartoons depict gp120/gp41 trimers, trimer-IgG complexes, and monomers. Markers show the locations of ferritin markers of 220 and 439 kDa and trimers complexed with one, two, or three molecules of IgG 2G12 (molecular masses of ∼570, ∼720, and ∼870 kDa). Arrows indicate the dilution corresponding to the ID 50 titer, inferred from at least three duplicate titrations. The unbound fraction of plasma BB34 absorbed against WT gp120 beads was also evaluated for plasma BB34, identified as “F/TH,” flowthrough. (−), blank beads.

    Journal: Journal of Virology

    Article Title: Profiling the Specificity of Neutralizing Antibodies in a Large Panel of Plasmas from Patients Chronically Infected with Human Immunodeficiency Virus Type 1 Subtypes B and C ▿Profiling the Specificity of Neutralizing Antibodies in a Large Panel of Plasmas from Patients Chronically Infected with Human Immunodeficiency Virus Type 1 Subtypes B and C ▿ †

    doi: 10.1128/JVI.01762-08

    Figure Lengend Snippet: Evaluation of plasma binding to WT and mutant trimers by BN-PAGE. Plasmas were titrated against WT, D368R, or N295A trimers in BN-PAGE as indicated. Cartoons depict gp120/gp41 trimers, trimer-IgG complexes, and monomers. Markers show the locations of ferritin markers of 220 and 439 kDa and trimers complexed with one, two, or three molecules of IgG 2G12 (molecular masses of ∼570, ∼720, and ∼870 kDa). Arrows indicate the dilution corresponding to the ID 50 titer, inferred from at least three duplicate titrations. The unbound fraction of plasma BB34 absorbed against WT gp120 beads was also evaluated for plasma BB34, identified as “F/TH,” flowthrough. (−), blank beads.

    Article Snippet: The concentration of isotype- and subclass-specific Ab was calculated by linear regression using a concentration curve of purified IgG1, IgG2, IgG3, IgG4, IgA, or IgM proteins (Sigma) in each plate, allowing a comparison of the relative levels of plasma Abs.

    Techniques: Binding Assay, Mutagenesis, Polyacrylamide Gel Electrophoresis

    IgG subclass specificity of gingipain K. A ) Human IgG subclasses 1–4 were incubated with gingipain K for 4 h at 37°C and visualized by SDS-PAGE under reducing conditions. From densitometric scanning, molecular masses of IgG heavy and light

    Journal: The FASEB Journal

    Article Title: Cleavage of IgG1 and IgG3 by gingipain K from Porphyromonas gingivalis may compromise host defense in progressive periodontitis

    doi: 10.1096/fj.11-187799

    Figure Lengend Snippet: IgG subclass specificity of gingipain K. A ) Human IgG subclasses 1–4 were incubated with gingipain K for 4 h at 37°C and visualized by SDS-PAGE under reducing conditions. From densitometric scanning, molecular masses of IgG heavy and light

    Article Snippet: Determination of cleaved IgG1 and IgG2 in GCF samples was performed by Western blot analysis using mouse mAb to human IgG1 and IgG2 (Zymed Labs, San Francisco, CA, USA).

    Techniques: Incubation, SDS Page

    Analysis of IgG 1 hydrolysis in patients suffering from periodontitis. GCF samples were collected from 6 patients with chronic periodontitis characterized with regard to PD and BoP (− or +). The samples were analyzed for the P. gingivalis load

    Journal: The FASEB Journal

    Article Title: Cleavage of IgG1 and IgG3 by gingipain K from Porphyromonas gingivalis may compromise host defense in progressive periodontitis

    doi: 10.1096/fj.11-187799

    Figure Lengend Snippet: Analysis of IgG 1 hydrolysis in patients suffering from periodontitis. GCF samples were collected from 6 patients with chronic periodontitis characterized with regard to PD and BoP (− or +). The samples were analyzed for the P. gingivalis load

    Article Snippet: Determination of cleaved IgG1 and IgG2 in GCF samples was performed by Western blot analysis using mouse mAb to human IgG1 and IgG2 (Zymed Labs, San Francisco, CA, USA).

    Techniques:

    Enzyme kinetic investigations of the gingipain K interaction with human IgG. Whole human IgG was used as a substrate to reflect the overall effect of gingipain K hydrolysis. Since gingipain K does not display Michaelis-Menten enzyme kinetics, it is not

    Journal: The FASEB Journal

    Article Title: Cleavage of IgG1 and IgG3 by gingipain K from Porphyromonas gingivalis may compromise host defense in progressive periodontitis

    doi: 10.1096/fj.11-187799

    Figure Lengend Snippet: Enzyme kinetic investigations of the gingipain K interaction with human IgG. Whole human IgG was used as a substrate to reflect the overall effect of gingipain K hydrolysis. Since gingipain K does not display Michaelis-Menten enzyme kinetics, it is not

    Article Snippet: Determination of cleaved IgG1 and IgG2 in GCF samples was performed by Western blot analysis using mouse mAb to human IgG1 and IgG2 (Zymed Labs, San Francisco, CA, USA).

    Techniques:

    ( A – H ) High-avidity PRRSV specific and virus-neutralizing (VN) antibodies were produced in pigs vaccinated with adjuvanted NP-KAg. Pigs were vaccinated or unvaccinated with indicated vaccine and adjuvant combination and challenged with PRRSV MN184. The plasma samples collected from pigs of 100 μg per pig vaccine dose ( A – C ) or 500 μg dose category ( E – G ) on the day of challenge (PC 0), followed by PC 6 and PC 15 (day of necropsy), were analyzed for avidity of PRRSV-specific IgG by ELISA. Each symbol indicates the mean of percentage absorbance compared to no ammonium thiocyanate (0 M) ± SEM of three pigs. The VN antibody titers in the plasma samples of 100 μg per pig vaccine dose ( D ) or 500 μg dose category ( H ) were analyzed by indirect immunofluorescence assay. Each symbol in the line graph indicates the average VN titer of three pigs ± SEM at indicated time point of blood collection. Lowercase letters indicate statistically significant ( P

    Journal: International Journal of Nanomedicine

    Article Title: Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs

    doi: 10.2147/IJN.S56127

    Figure Lengend Snippet: ( A – H ) High-avidity PRRSV specific and virus-neutralizing (VN) antibodies were produced in pigs vaccinated with adjuvanted NP-KAg. Pigs were vaccinated or unvaccinated with indicated vaccine and adjuvant combination and challenged with PRRSV MN184. The plasma samples collected from pigs of 100 μg per pig vaccine dose ( A – C ) or 500 μg dose category ( E – G ) on the day of challenge (PC 0), followed by PC 6 and PC 15 (day of necropsy), were analyzed for avidity of PRRSV-specific IgG by ELISA. Each symbol indicates the mean of percentage absorbance compared to no ammonium thiocyanate (0 M) ± SEM of three pigs. The VN antibody titers in the plasma samples of 100 μg per pig vaccine dose ( D ) or 500 μg dose category ( H ) were analyzed by indirect immunofluorescence assay. Each symbol in the line graph indicates the average VN titer of three pigs ± SEM at indicated time point of blood collection. Lowercase letters indicate statistically significant ( P

    Article Snippet: Briefly killed semipurified PRRSV (MN184) Ags (5 μg/mL)-coated plates were blocked and plated with a serial tenfold dilution of plasma samples, and the plate-bound virus-specific IgG1 and IgG2 were detected using mouse antipig IgG1 and IgG2 (AbD Serotec; Bio-Rad Laboratories, Hercules, CA, USA) (1:250 dilution), respectively.

    Techniques: Produced, Enzyme-linked Immunosorbent Assay, Immunofluorescence

    ( A – D ) Balanced Th1–Th2 antibody levels in pigs vaccinated with adjuvanted NP-KAg vaccine. Pigs were vaccinated or unvaccinated with indicated vaccine and adjuvant combination and challenged with PRRSV MN184. Plasma samples collected at indicated PCs were analyzed for virus-specific IgG 1 or IgG 2 ( A and B ) levels by ELISA. The ratio of Th2:Th1 response was estimated ( C and D ). Each bar/symbol indicates the mean titer/average OD value/ratio of indicated antibody types from three pigs ± SEM. Lowercase letters indicate statistically significant ( P

    Journal: International Journal of Nanomedicine

    Article Title: Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs

    doi: 10.2147/IJN.S56127

    Figure Lengend Snippet: ( A – D ) Balanced Th1–Th2 antibody levels in pigs vaccinated with adjuvanted NP-KAg vaccine. Pigs were vaccinated or unvaccinated with indicated vaccine and adjuvant combination and challenged with PRRSV MN184. Plasma samples collected at indicated PCs were analyzed for virus-specific IgG 1 or IgG 2 ( A and B ) levels by ELISA. The ratio of Th2:Th1 response was estimated ( C and D ). Each bar/symbol indicates the mean titer/average OD value/ratio of indicated antibody types from three pigs ± SEM. Lowercase letters indicate statistically significant ( P

    Article Snippet: Briefly killed semipurified PRRSV (MN184) Ags (5 μg/mL)-coated plates were blocked and plated with a serial tenfold dilution of plasma samples, and the plate-bound virus-specific IgG1 and IgG2 were detected using mouse antipig IgG1 and IgG2 (AbD Serotec; Bio-Rad Laboratories, Hercules, CA, USA) (1:250 dilution), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay

    ( A – H ) Significant increase in PRRSV-specific total, and M- and N-specific IgG in pigs vaccinated with adjuvanted NP-KAg. Pigs were vaccinated or unvaccinated with indicated vaccine and adjuvant combination and challenged with PRRSV MN184. The plasma samples collected at indicated PCs were analyzed for specific IgG against PRRSV total proteins ( A and B ), GP5 ( C and D ), M ( E and F ), and N ( G and H ) by ELISA. Notes: Each symbol indicates the mean PRRSV-specific IgG titer ± SEM of three pigs of the indicated group. Lowercase letters indicate statistically significant ( P

    Journal: International Journal of Nanomedicine

    Article Title: Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs

    doi: 10.2147/IJN.S56127

    Figure Lengend Snippet: ( A – H ) Significant increase in PRRSV-specific total, and M- and N-specific IgG in pigs vaccinated with adjuvanted NP-KAg. Pigs were vaccinated or unvaccinated with indicated vaccine and adjuvant combination and challenged with PRRSV MN184. The plasma samples collected at indicated PCs were analyzed for specific IgG against PRRSV total proteins ( A and B ), GP5 ( C and D ), M ( E and F ), and N ( G and H ) by ELISA. Notes: Each symbol indicates the mean PRRSV-specific IgG titer ± SEM of three pigs of the indicated group. Lowercase letters indicate statistically significant ( P

    Article Snippet: Briefly killed semipurified PRRSV (MN184) Ags (5 μg/mL)-coated plates were blocked and plated with a serial tenfold dilution of plasma samples, and the plate-bound virus-specific IgG1 and IgG2 were detected using mouse antipig IgG1 and IgG2 (AbD Serotec; Bio-Rad Laboratories, Hercules, CA, USA) (1:250 dilution), respectively.

    Techniques: Enzyme-linked Immunosorbent Assay

    Purification of GP300. GP300 was purified from water-insoluble extract by use of WGA affinity chromatography and analyzed by SDS-PAGE and silver staining (A) and Western blotting with IgG1 from sera of infected animals (B). Materials loaded onto the gel:

    Journal:

    Article Title: Antibodies Elicited by the Bovine Lungworm, Dictyocaulus viviparus, Cross-React with Platelet-Activating Factor ▿

    doi: 10.1128/IAI.00633-07

    Figure Lengend Snippet: Purification of GP300. GP300 was purified from water-insoluble extract by use of WGA affinity chromatography and analyzed by SDS-PAGE and silver staining (A) and Western blotting with IgG1 from sera of infected animals (B). Materials loaded onto the gel:

    Article Snippet: Binding of Igs was determined using one of the following isotype-specific mAbs in combination with goat anti-mouse Ig-AP (D0486, 1:2,000 dilution; DAKO) and the pNPP kit: anti-IgG1 (mca 627; Serotec), anti-IgA (mca 628; Serotec), and anti-IgG2 (mca 626; Serotec) (all at 1:1,000 dilution).

    Techniques: Purification, Whole Genome Amplification, Affinity Chromatography, SDS Page, Silver Staining, Western Blot, Infection

    Extraction of GP300. (A) Western blot demonstrating the reactivities of ES (lane 1), water-insoluble extract (lane 2), and water-soluble extract (lane 3) of adult worms (7.5 μg protein/lane) with IgG1 from D. viviparus -infected calves. Sera from

    Journal:

    Article Title: Antibodies Elicited by the Bovine Lungworm, Dictyocaulus viviparus, Cross-React with Platelet-Activating Factor ▿

    doi: 10.1128/IAI.00633-07

    Figure Lengend Snippet: Extraction of GP300. (A) Western blot demonstrating the reactivities of ES (lane 1), water-insoluble extract (lane 2), and water-soluble extract (lane 3) of adult worms (7.5 μg protein/lane) with IgG1 from D. viviparus -infected calves. Sera from

    Article Snippet: Binding of Igs was determined using one of the following isotype-specific mAbs in combination with goat anti-mouse Ig-AP (D0486, 1:2,000 dilution; DAKO) and the pNPP kit: anti-IgG1 (mca 627; Serotec), anti-IgA (mca 628; Serotec), and anti-IgG2 (mca 626; Serotec) (all at 1:1,000 dilution).

    Techniques: Western Blot, Infection

    Correlation between protection against infection and the GP300-specific IgG2/IgG1 ratio at day 54. The ratio of the GP300-specific IgG2 and IgG1 levels was calculated from the data presented in Fig. (mock-treated GP300). The correlation

    Journal:

    Article Title: Antibodies Elicited by the Bovine Lungworm, Dictyocaulus viviparus, Cross-React with Platelet-Activating Factor ▿

    doi: 10.1128/IAI.00633-07

    Figure Lengend Snippet: Correlation between protection against infection and the GP300-specific IgG2/IgG1 ratio at day 54. The ratio of the GP300-specific IgG2 and IgG1 levels was calculated from the data presented in Fig. (mock-treated GP300). The correlation

    Article Snippet: Binding of Igs was determined using one of the following isotype-specific mAbs in combination with goat anti-mouse Ig-AP (D0486, 1:2,000 dilution; DAKO) and the pNPP kit: anti-IgG1 (mca 627; Serotec), anti-IgA (mca 628; Serotec), and anti-IgG2 (mca 626; Serotec) (all at 1:1,000 dilution).

    Techniques: Infection

    Anti- S. aureus IgG1 and IgG2 titers in paired maternal and cord serum samples from the case and control groups. (a) Anti- S. aureus IgG1 titers and (d) anti- S. aureus IgG2 titers in the maternal serum and umbilical cord serum samples from the case (n=48) and control group (n=98). In (b) and (c) , correlation coefficients between paired maternal and umbilical cord serum of anti- Sa IgG1 in the case and control group, respectively. In (e) and (f) , correlation coefficients between the paired maternal and umbilical cord serum of anti- Sa IgG2 in the case and control group, respectively. In (a) and (d) , the box represents the 25 th –75 th percentiles, and the median is represented by the line within the box. The whiskers represent the 5 th –95 th percentiles.

    Journal: Clinics

    Article Title: Passive acquisition of anti-Staphylococcus aureus antibodies by newborns via transplacental transfer and breastfeeding, regardless of maternal colonization

    doi: 10.6061/clinics/2016(12)02

    Figure Lengend Snippet: Anti- S. aureus IgG1 and IgG2 titers in paired maternal and cord serum samples from the case and control groups. (a) Anti- S. aureus IgG1 titers and (d) anti- S. aureus IgG2 titers in the maternal serum and umbilical cord serum samples from the case (n=48) and control group (n=98). In (b) and (c) , correlation coefficients between paired maternal and umbilical cord serum of anti- Sa IgG1 in the case and control group, respectively. In (e) and (f) , correlation coefficients between the paired maternal and umbilical cord serum of anti- Sa IgG2 in the case and control group, respectively. In (a) and (d) , the box represents the 25 th –75 th percentiles, and the median is represented by the line within the box. The whiskers represent the 5 th –95 th percentiles.

    Article Snippet: Biotinylated anti-human IgG1 (555869; BD Pharmingen, San Diego, CA, USA) or IgG2 (555874; BD Pharmingen, San Diego, CA, USA) was used as the secondary antibody, both at dilutions of 1:500, and incubated for 90 minutes.

    Techniques:

    Placental transfer ratios (%) of anti- S. aureus IgG1 and IgG2 from case and control groups. Comparison of the data is presented as box and whisker plots. The box represents the 25 th –75 th percentiles, and the median is represented by the line within the box. The whiskers represent the 5 th –95 th percentiles.

    Journal: Clinics

    Article Title: Passive acquisition of anti-Staphylococcus aureus antibodies by newborns via transplacental transfer and breastfeeding, regardless of maternal colonization

    doi: 10.6061/clinics/2016(12)02

    Figure Lengend Snippet: Placental transfer ratios (%) of anti- S. aureus IgG1 and IgG2 from case and control groups. Comparison of the data is presented as box and whisker plots. The box represents the 25 th –75 th percentiles, and the median is represented by the line within the box. The whiskers represent the 5 th –95 th percentiles.

    Article Snippet: Biotinylated anti-human IgG1 (555869; BD Pharmingen, San Diego, CA, USA) or IgG2 (555874; BD Pharmingen, San Diego, CA, USA) was used as the secondary antibody, both at dilutions of 1:500, and incubated for 90 minutes.

    Techniques: Whisker Assay

    Antigenic characterization of Env IL-4 and Env IL-21 molecules. ELISA reactivity of Env IL-4 and Env IL-21 with 2G12 and HIV-Ig (A); b12 and CD4-IgG2 (B); and 48d (CD4i) in the absence and presence of sCD4 at 1 µg/ml (C). All ELISA results are representative for at least three independent experiments using proteins derived from three independent transfections.

    Journal: PLoS ONE

    Article Title: An HIV-1 Envelope Glycoprotein Trimer with an Embedded IL-21 Domain Activates Human B Cells

    doi: 10.1371/journal.pone.0067309

    Figure Lengend Snippet: Antigenic characterization of Env IL-4 and Env IL-21 molecules. ELISA reactivity of Env IL-4 and Env IL-21 with 2G12 and HIV-Ig (A); b12 and CD4-IgG2 (B); and 48d (CD4i) in the absence and presence of sCD4 at 1 µg/ml (C). All ELISA results are representative for at least three independent experiments using proteins derived from three independent transfections.

    Article Snippet: CD4-IgG2, sCD4 and anti-V3 gp120 MAb PA1 were gifts from Bill Olson (Progenics Pharmaceutical, Tarrytown, NY).

    Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Transfection

    Comparison of the levels of the apoptotic marker CC3 (A,C) and the proliferation marker Ki67 (B,D) in the tumors of the mice passively immunized with the rabbit IgG against the mPD1-derived mimotope, or in the mice actively immunized with the mimotope, evaluated by IHC staining. For each tumor, more than one region was quantified for detection of evaluated markers. Representative images are shown. Significant differences are indicated by asterisks (* P

    Journal: Frontiers in Immunology

    Article Title: A New Strategy Toward B Cell-Based Cancer Vaccines by Active Immunization With Mimotopes of Immune Checkpoint Inhibitors

    doi: 10.3389/fimmu.2020.00895

    Figure Lengend Snippet: Comparison of the levels of the apoptotic marker CC3 (A,C) and the proliferation marker Ki67 (B,D) in the tumors of the mice passively immunized with the rabbit IgG against the mPD1-derived mimotope, or in the mice actively immunized with the mimotope, evaluated by IHC staining. For each tumor, more than one region was quantified for detection of evaluated markers. Representative images are shown. Significant differences are indicated by asterisks (* P

    Article Snippet: Bound mAbs to the coated recombinant proteins were detected using mouse anti-rat IgG (against anti-mPD1 mAb; HRP-conjugated; Jackson ImmunoResearch, Cambridgeshire, UK) or mouse anti-human IgG (Fc; against the anti-hPD1 mAb; HRP-conjugated; SouthernBiotech, Birmingham, AL, USA) as secondary antibodies.

    Techniques: Marker, Mouse Assay, Derivative Assay, Immunohistochemistry, Staining

    Evaluation of anti-tumor capacity in vivo by passive immunization with the rabbit IgG against JT–mPD1 in a syngeneic tumor mouse model. (A) Inhibition ELISA showing the binding of mPD–L1 to coated mPD1, before or after preincubation with control rabbit IgG or with different concentrations of rabbit-specific IgG against JT–mPD1 n = 4 for each data point. (B) BALB/c mice either remained untreated (naïve) or were injected as depicted. (C) In vivo anti-tumor effect shown by bars expressing the weight of the tumors explanted upon sacrifice from all the mice in each group. The levels of tumor growth reduction in the mice immunized with the rabbit IgG Abs or the examined mAb against mPD1, compared to the naïve mice, are indicated in percentages above the respective bars. Corresponding macroscopic images of representative explanted tumors are shown below each bar n = 8 for each data point. The results are representative of at least two repeated experiments. Significant differences are indicated by asterisks (** P

    Journal: Frontiers in Immunology

    Article Title: A New Strategy Toward B Cell-Based Cancer Vaccines by Active Immunization With Mimotopes of Immune Checkpoint Inhibitors

    doi: 10.3389/fimmu.2020.00895

    Figure Lengend Snippet: Evaluation of anti-tumor capacity in vivo by passive immunization with the rabbit IgG against JT–mPD1 in a syngeneic tumor mouse model. (A) Inhibition ELISA showing the binding of mPD–L1 to coated mPD1, before or after preincubation with control rabbit IgG or with different concentrations of rabbit-specific IgG against JT–mPD1 n = 4 for each data point. (B) BALB/c mice either remained untreated (naïve) or were injected as depicted. (C) In vivo anti-tumor effect shown by bars expressing the weight of the tumors explanted upon sacrifice from all the mice in each group. The levels of tumor growth reduction in the mice immunized with the rabbit IgG Abs or the examined mAb against mPD1, compared to the naïve mice, are indicated in percentages above the respective bars. Corresponding macroscopic images of representative explanted tumors are shown below each bar n = 8 for each data point. The results are representative of at least two repeated experiments. Significant differences are indicated by asterisks (** P

    Article Snippet: Bound mAbs to the coated recombinant proteins were detected using mouse anti-rat IgG (against anti-mPD1 mAb; HRP-conjugated; Jackson ImmunoResearch, Cambridgeshire, UK) or mouse anti-human IgG (Fc; against the anti-hPD1 mAb; HRP-conjugated; SouthernBiotech, Birmingham, AL, USA) as secondary antibodies.

    Techniques: In Vivo, Inhibition, Enzyme-linked Immunosorbent Assay, Binding Assay, Mouse Assay, Injection, Expressing

    Evaluation of anti-tumor capacity in vivo by active immunization with JT–mPD1 in a syngeneic tumor mouse model. (A) BALB/c mice either remained untreated (naïve) or were immunized as depicted. (B) Level of serum IgG antibody responses against recombinant mPD1 protein, at the time of the sacrifice of the mice immunized with the mimotope. (C) In vivo anti-tumor effect shown by bars expressing the weight of the tumors explanted upon sacrifice from all the mice in each group. The level of tumor growth reduction in the immunized mice, compared to the naïve mice, is indicated in percentages above the respective bar. Corresponding macroscopic images of representative explanted tumors are shown below each bar n = 10 for each data point. The results are representative of at least two repeated experiments. Significant differences are indicated by asterisks (* P

    Journal: Frontiers in Immunology

    Article Title: A New Strategy Toward B Cell-Based Cancer Vaccines by Active Immunization With Mimotopes of Immune Checkpoint Inhibitors

    doi: 10.3389/fimmu.2020.00895

    Figure Lengend Snippet: Evaluation of anti-tumor capacity in vivo by active immunization with JT–mPD1 in a syngeneic tumor mouse model. (A) BALB/c mice either remained untreated (naïve) or were immunized as depicted. (B) Level of serum IgG antibody responses against recombinant mPD1 protein, at the time of the sacrifice of the mice immunized with the mimotope. (C) In vivo anti-tumor effect shown by bars expressing the weight of the tumors explanted upon sacrifice from all the mice in each group. The level of tumor growth reduction in the immunized mice, compared to the naïve mice, is indicated in percentages above the respective bar. Corresponding macroscopic images of representative explanted tumors are shown below each bar n = 10 for each data point. The results are representative of at least two repeated experiments. Significant differences are indicated by asterisks (* P

    Article Snippet: Bound mAbs to the coated recombinant proteins were detected using mouse anti-rat IgG (against anti-mPD1 mAb; HRP-conjugated; Jackson ImmunoResearch, Cambridgeshire, UK) or mouse anti-human IgG (Fc; against the anti-hPD1 mAb; HRP-conjugated; SouthernBiotech, Birmingham, AL, USA) as secondary antibodies.

    Techniques: In Vivo, Mouse Assay, Recombinant, Expressing

    Anti-rhGAA IgG1 and IgG4 are the most prevalent subclasses of antibodies developed in response to long-term ERT. ( A – F ) Anti-rhGAA antibodies measured with a capture assay specific for IgG1, IgG2, IgG3, IgG4, IgM, and IgE antibodies. Estimated antibody concentrations are reported for each subject. Average and standard deviation are indicated for each of the study cohorts: LOPD subjects receiving ERT (Treated, n = 28), untreated LOPD subjects (Untreated, n = 10), and healthy donors (HD, n = 43). Unpaired two-tailed t-test was used to compare results across the study cohorts (**P

    Journal: Scientific Reports

    Article Title: Long-term exposure to Myozyme results in a decrease of anti-drug antibodies in late-onset Pompe disease patients

    doi: 10.1038/srep36182

    Figure Lengend Snippet: Anti-rhGAA IgG1 and IgG4 are the most prevalent subclasses of antibodies developed in response to long-term ERT. ( A – F ) Anti-rhGAA antibodies measured with a capture assay specific for IgG1, IgG2, IgG3, IgG4, IgM, and IgE antibodies. Estimated antibody concentrations are reported for each subject. Average and standard deviation are indicated for each of the study cohorts: LOPD subjects receiving ERT (Treated, n = 28), untreated LOPD subjects (Untreated, n = 10), and healthy donors (HD, n = 43). Unpaired two-tailed t-test was used to compare results across the study cohorts (**P

    Article Snippet: A standard curve made of purified human IgG (Gamunex, Grifolds, Meyreuil, France), purified human IgG1 (Life Technologies, Saint Aubin, France), IgG2, IgG3, IgG4, IgE (Abcam, Paris, France), or IgM (Sigma-Aldrich, Lyon, France) was added directly to the plates.

    Techniques: Standard Deviation, Two Tailed Test

    Profiles of the FMDV-ASC detected in B 2 T- and B 4 T-vaccinated cattle after FMDV challenge. (a) Mononuclear cells were purified from mandibular lymph nodes (ML), medial retropharyngeal lymph nodes (MRL), and tracheobronchial lymph nodes (TBL) and characterized by the FMDV-ASC ELISPOT assay, using monoclonal (IgG1 and IgG2) or polyclonal (IgM and IgA) antibodies against bovine immunoglobulin isotypes as probes. (b) Total FMDV-ASC in ML, MRL, or TBL. Results are expressed as the mean number of FMDV-specific ASC per 1 × 10 6 extracted cells. Each bar represents the mean value of 3 replicates ± SD. PPG: protected against podal generalization; PP: partial protected; NP: non-protected.

    Journal: Journal of Immunology Research

    Article Title: Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle

    doi: 10.1155/2018/3497401

    Figure Lengend Snippet: Profiles of the FMDV-ASC detected in B 2 T- and B 4 T-vaccinated cattle after FMDV challenge. (a) Mononuclear cells were purified from mandibular lymph nodes (ML), medial retropharyngeal lymph nodes (MRL), and tracheobronchial lymph nodes (TBL) and characterized by the FMDV-ASC ELISPOT assay, using monoclonal (IgG1 and IgG2) or polyclonal (IgM and IgA) antibodies against bovine immunoglobulin isotypes as probes. (b) Total FMDV-ASC in ML, MRL, or TBL. Results are expressed as the mean number of FMDV-specific ASC per 1 × 10 6 extracted cells. Each bar represents the mean value of 3 replicates ± SD. PPG: protected against podal generalization; PP: partial protected; NP: non-protected.

    Article Snippet: After 5 washes with phosphate-buffered saline (PBS), mouse anti-bovine IgG1 or IgG2 monoclonal antibodies (BD-Serotec, Oxford, UK) were added (1 : 500 dilution) and incubated for 1 h at RT.

    Techniques: Purification, Enzyme-linked Immunospot

    Antibody detection by ELISA in vaccinated cattle. Animals were immunized on days 0 and 18 (arrow) with B 2 T or B 4 T vaccine. (a) Kinetics of anti-peptide serum antibodies. Bars represent the mean IgG titres from bovines in each group (gray, B 2 T; black, B 4 T) throughout the experiment (error marks, SD). (b, c) Kinetics of total IgG and IgG1 anti-FMDV O1/Campos/Bra/58 serum antibodies. Titres were calculated as log 10 of the last reciprocal dilution above cut-off. Data points represent the IgG titre (b) or IgG1 titre (c) from each animal represented by different shapes (right legend) throughout the experiment. (d) FMDV-specific mucosal IgG1 and IgA responses. Nasal swabs were collected at 22 dpv. Each point represents the nasal IgG1 anti-FMDV antibody titres (log 10 ) (black) or IgA (gray) anti-FMDV O1/Campos/Bra/58 antibody level of each animal. The cut-off was established as the mean value of mock-vaccinated animals plus twice the SD value (dotted line).

    Journal: Journal of Immunology Research

    Article Title: Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle

    doi: 10.1155/2018/3497401

    Figure Lengend Snippet: Antibody detection by ELISA in vaccinated cattle. Animals were immunized on days 0 and 18 (arrow) with B 2 T or B 4 T vaccine. (a) Kinetics of anti-peptide serum antibodies. Bars represent the mean IgG titres from bovines in each group (gray, B 2 T; black, B 4 T) throughout the experiment (error marks, SD). (b, c) Kinetics of total IgG and IgG1 anti-FMDV O1/Campos/Bra/58 serum antibodies. Titres were calculated as log 10 of the last reciprocal dilution above cut-off. Data points represent the IgG titre (b) or IgG1 titre (c) from each animal represented by different shapes (right legend) throughout the experiment. (d) FMDV-specific mucosal IgG1 and IgA responses. Nasal swabs were collected at 22 dpv. Each point represents the nasal IgG1 anti-FMDV antibody titres (log 10 ) (black) or IgA (gray) anti-FMDV O1/Campos/Bra/58 antibody level of each animal. The cut-off was established as the mean value of mock-vaccinated animals plus twice the SD value (dotted line).

    Article Snippet: After 5 washes with phosphate-buffered saline (PBS), mouse anti-bovine IgG1 or IgG2 monoclonal antibodies (BD-Serotec, Oxford, UK) were added (1 : 500 dilution) and incubated for 1 h at RT.

    Techniques: Enzyme-linked Immunosorbent Assay

    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum IgG ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.

    Journal: PLoS ONE

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes

    doi: 10.1371/journal.pone.0203665

    Figure Lengend Snippet: Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serum IgG ofdifferent individuals to different microorganism, obtained in ELISA, at steady state. A) Professional athletes; B) Control group. Red colour–Pearson product-moment correlation coefficient, r = 1, Blue– r = -1.

    Article Snippet: For IgG subclass analysis, Biotin Mouse Anti-Human IgG1, IgG2, IgG4 (BD Biosciences, CA, USA), and Monoclonal Anti-Human IgG3-Biotin antibody produced in mouse (Sigma Aldrich) were used.All secondary antibodies were incubated for 1 hour at RT.

    Techniques: Enzyme-linked Immunosorbent Assay

    Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serumtotal IgG and total IgA with IgG subclasses, for different microorganisms in professional athletes. A) Escherichia coli ATCC25922; B) Candida albicans ATCC 10259; C) Lactobacillus plantarum WCFS1; D) Salmonella typhimurium 2865; E) Lactobacillusrhamnosus LGG, F) LPS from E . coli O55:B5.

    Journal: PLoS ONE

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes

    doi: 10.1371/journal.pone.0203665

    Figure Lengend Snippet: Graphic representation showing correlation coefficients obtained by correlating the string of reactivity of serumtotal IgG and total IgA with IgG subclasses, for different microorganisms in professional athletes. A) Escherichia coli ATCC25922; B) Candida albicans ATCC 10259; C) Lactobacillus plantarum WCFS1; D) Salmonella typhimurium 2865; E) Lactobacillusrhamnosus LGG, F) LPS from E . coli O55:B5.

    Article Snippet: For IgG subclass analysis, Biotin Mouse Anti-Human IgG1, IgG2, IgG4 (BD Biosciences, CA, USA), and Monoclonal Anti-Human IgG3-Biotin antibody produced in mouse (Sigma Aldrich) were used.All secondary antibodies were incubated for 1 hour at RT.

    Techniques:

    Analysis of reactivity of serial dilutions of serum IgG in A) professional athletes; B) control group, and of serum IgA in C) professional athletes; D) control group to different microorganisms. Red colour– E . coli ATCC25922; black - C . albicans ATCC 10259; turquoise– L . plantarum WCFS1; green– S . typhimurium 2865; blue— L . rhamnosus LGG, violet—LPS from E . coli 055:B5.

    Journal: PLoS ONE

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes

    doi: 10.1371/journal.pone.0203665

    Figure Lengend Snippet: Analysis of reactivity of serial dilutions of serum IgG in A) professional athletes; B) control group, and of serum IgA in C) professional athletes; D) control group to different microorganisms. Red colour– E . coli ATCC25922; black - C . albicans ATCC 10259; turquoise– L . plantarum WCFS1; green– S . typhimurium 2865; blue— L . rhamnosus LGG, violet—LPS from E . coli 055:B5.

    Article Snippet: For IgG subclass analysis, Biotin Mouse Anti-Human IgG1, IgG2, IgG4 (BD Biosciences, CA, USA), and Monoclonal Anti-Human IgG3-Biotin antibody produced in mouse (Sigma Aldrich) were used.All secondary antibodies were incubated for 1 hour at RT.

    Techniques:

    The strings of reactivities (apsorbance) of IgG and IgA to bacteria from different individuals were correlated, and the Pearson product-moment correlation coefficientswereplotted for individual bacteria. Red colour- Professional athletes PA; Green colour–Control.

    Journal: PLoS ONE

    Article Title: Profiling of microorganism-binding serum antibody specificities in professional athletes

    doi: 10.1371/journal.pone.0203665

    Figure Lengend Snippet: The strings of reactivities (apsorbance) of IgG and IgA to bacteria from different individuals were correlated, and the Pearson product-moment correlation coefficientswereplotted for individual bacteria. Red colour- Professional athletes PA; Green colour–Control.

    Article Snippet: For IgG subclass analysis, Biotin Mouse Anti-Human IgG1, IgG2, IgG4 (BD Biosciences, CA, USA), and Monoclonal Anti-Human IgG3-Biotin antibody produced in mouse (Sigma Aldrich) were used.All secondary antibodies were incubated for 1 hour at RT.

    Techniques:

    Dogs immunized with the Lu. longipalpis salivary molecules LJM17 or LJL143 develop strong and specific humoral and cellular immune responses. (A) Total IgG and (B) IgG 1 and IgG 2 antibody levels up to day 224 in dogs immunized with either LJM17 (n = 5), LJL143 (n = 5) or the empty plasmid (n = 5). LJL143- and LJM17-immunized dogs were tested using the appropriate recombinant proteins (LJL143 and LJM17). Dogs immunized with the empty vector (control dogs) were tested against both recombinant proteins for IgG, (LJL143-control, LJM17-control), IgG 1 (IgG 1 -control) and IgG 2 (IgG 2 -control). (C) In vitro IFN-γ production by PBMC from LJL143- and LJM17-immunized and control dogs stimulated with media (Med), ConcavalinA (ConA), salivary gland homogenate (SGH), rLJM17 or rLJL143 two weeks after the final vaccination. Error bars represent means±S.E.

    Journal: PLoS Pathogens

    Article Title: Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania

    doi: 10.1371/journal.ppat.1000441

    Figure Lengend Snippet: Dogs immunized with the Lu. longipalpis salivary molecules LJM17 or LJL143 develop strong and specific humoral and cellular immune responses. (A) Total IgG and (B) IgG 1 and IgG 2 antibody levels up to day 224 in dogs immunized with either LJM17 (n = 5), LJL143 (n = 5) or the empty plasmid (n = 5). LJL143- and LJM17-immunized dogs were tested using the appropriate recombinant proteins (LJL143 and LJM17). Dogs immunized with the empty vector (control dogs) were tested against both recombinant proteins for IgG, (LJL143-control, LJM17-control), IgG 1 (IgG 1 -control) and IgG 2 (IgG 2 -control). (C) In vitro IFN-γ production by PBMC from LJL143- and LJM17-immunized and control dogs stimulated with media (Med), ConcavalinA (ConA), salivary gland homogenate (SGH), rLJM17 or rLJL143 two weeks after the final vaccination. Error bars represent means±S.E.

    Article Snippet: After three washes with PBS-T, sheep anti-dog IgG (1∶5000), goat anti-dog IgG1 (1∶500) or sheep anti-dog IgG2 (1∶500) phosphatase alkaline-conjugated antibodies ( Bethyl Laboratories Inc.) were incubated for 1 h at 37°C.

    Techniques: Plasmid Preparation, Recombinant, In Vitro

    Dogs develop a strong humoral and cellular immune response to bites of Lu. longipalpis sand flies. Dogs (n = 9) were exposed for 10 min to bites of 20 sand flies three times at one week intervals (first exposure, E1; second exposure, E2; third exposure, E3). (A) Weekly measurement of IgG, IgG 1 and IgG 2 antibody levels in dogs exposed to sand flies. (B) Induration score in a representative dog 48 h after each of three sand fly exposures. The induration score is an arbitrary scale corresponding to the area of induration and redness resulting from multiple bites where 1≤1 cm 2 , 2≤5 cm 2 , 3≤10 cm 2 and 4 > 10 cm 2 . (C) Representative H E staining of biopsies taken from sand fly bite sites prior to exposure (E0) and 48 h after each of three sand fly exposures (E1–E3). Note marked cellular infiltrate within dermis and thickening of epidermis in E3. (D) Immunohistochemical labeling of tissue sections from E3 demonstrating the presence of abundant CD3+T cells (CD3), macrophages (Mac387) and eosinophil granules (Luna stain).

    Journal: PLoS Pathogens

    Article Title: Sand Fly Salivary Proteins Induce Strong Cellular Immunity in a Natural Reservoir of Visceral Leishmaniasis with Adverse Consequences for Leishmania

    doi: 10.1371/journal.ppat.1000441

    Figure Lengend Snippet: Dogs develop a strong humoral and cellular immune response to bites of Lu. longipalpis sand flies. Dogs (n = 9) were exposed for 10 min to bites of 20 sand flies three times at one week intervals (first exposure, E1; second exposure, E2; third exposure, E3). (A) Weekly measurement of IgG, IgG 1 and IgG 2 antibody levels in dogs exposed to sand flies. (B) Induration score in a representative dog 48 h after each of three sand fly exposures. The induration score is an arbitrary scale corresponding to the area of induration and redness resulting from multiple bites where 1≤1 cm 2 , 2≤5 cm 2 , 3≤10 cm 2 and 4 > 10 cm 2 . (C) Representative H E staining of biopsies taken from sand fly bite sites prior to exposure (E0) and 48 h after each of three sand fly exposures (E1–E3). Note marked cellular infiltrate within dermis and thickening of epidermis in E3. (D) Immunohistochemical labeling of tissue sections from E3 demonstrating the presence of abundant CD3+T cells (CD3), macrophages (Mac387) and eosinophil granules (Luna stain).

    Article Snippet: After three washes with PBS-T, sheep anti-dog IgG (1∶5000), goat anti-dog IgG1 (1∶500) or sheep anti-dog IgG2 (1∶500) phosphatase alkaline-conjugated antibodies ( Bethyl Laboratories Inc.) were incubated for 1 h at 37°C.

    Techniques: Staining, Immunohistochemistry, Labeling

    Enzyme-linked immunosorbent assay (ELISA) anti-peptide IgG titers upon vaccination with a mixture of N, R, and C peptides. Panels show responses to mixtures using 150 μg total peptide dose in ( Row A ) Montanide ISA 720 or ( Row B ) Montanide ISA 51 and to mixtures using 300 μg total peptide dose in ( Row C ) Montanide ISA 720 or ( Row D ) Montanide ISA 51. Immunizations were made in the 0, 2, and 4 months.

    Journal: The American Journal of Tropical Medicine and Hygiene

    Article Title: Phase I Safety and Immunogenicity Trial of Plasmodium vivax CS Derived Long Synthetic Peptides Adjuvanted with Montanide ISA 720 or Montanide ISA 51

    doi: 10.4269/ajtmh.2011.09-0516

    Figure Lengend Snippet: Enzyme-linked immunosorbent assay (ELISA) anti-peptide IgG titers upon vaccination with a mixture of N, R, and C peptides. Panels show responses to mixtures using 150 μg total peptide dose in ( Row A ) Montanide ISA 720 or ( Row B ) Montanide ISA 51 and to mixtures using 300 μg total peptide dose in ( Row C ) Montanide ISA 720 or ( Row D ) Montanide ISA 51. Immunizations were made in the 0, 2, and 4 months.

    Article Snippet: Anti-IgG1, IgG3, and IgG4 (Skibio, Bedfordshire, England) and anti-IgG2 (Sigma, St. Louis, MO) isotypes were used and a peroxidase conjugated goat anti-mouse IgG antibody (Kirkegaard and Perry Laboratories, Gaithersburg, MD) was added and the reaction read at 630 nm (OD630).

    Techniques: Enzyme-linked Immunosorbent Assay

    Isolation of Nm proteins binding to human IgG by precipitation with Protein A or G beads. NmW135 strain A22 was cultured in CDM+HuS or MH as indicated. Unmarked lanes were intentionally left unloaded. The arrows indicate bands that produced a common set

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Identification of a phage-encoded immunoglobulin-binding protein from invasive Neisseria meningitidis

    doi: 10.4049/jimmunol.1301153

    Figure Lengend Snippet: Isolation of Nm proteins binding to human IgG by precipitation with Protein A or G beads. NmW135 strain A22 was cultured in CDM+HuS or MH as indicated. Unmarked lanes were intentionally left unloaded. The arrows indicate bands that produced a common set

    Article Snippet: Fab and Fc fragments of human IgG2 (Millipore) were prepared by incubating the mAb (5 mg/ml) with papain conjugated to agarose beads (Pierce Chemical Company, Rockford, IL) in 25 mM sodium phosphate buffer, pH 7, containing 2 mM cysteine at 37°C with constant mixing for 5 days.

    Techniques: Isolation, Binding Assay, Cell Culture, Produced

    Immunoglobulin-binding protein activity exhibited by diverse Nm strains. Panel a. shows IgG binding to diverse Nm strains with serum from two donors. Panel b. shows changes in IgG binding to Nm strains after each of three rounds of serial growth (indicated

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Identification of a phage-encoded immunoglobulin-binding protein from invasive Neisseria meningitidis

    doi: 10.4049/jimmunol.1301153

    Figure Lengend Snippet: Immunoglobulin-binding protein activity exhibited by diverse Nm strains. Panel a. shows IgG binding to diverse Nm strains with serum from two donors. Panel b. shows changes in IgG binding to Nm strains after each of three rounds of serial growth (indicated

    Article Snippet: Fab and Fc fragments of human IgG2 (Millipore) were prepared by incubating the mAb (5 mg/ml) with papain conjugated to agarose beads (Pierce Chemical Company, Rockford, IL) in 25 mM sodium phosphate buffer, pH 7, containing 2 mM cysteine at 37°C with constant mixing for 5 days.

    Techniques: Binding Assay, Activity Assay

    Immunoglobulin-binding activity of recombinant TspB derivatives by ELISA. Panel a. shows IgG-binding activity in whole serum from donor 2 of full length (FL) TspB1628 (filled circles), TspB1628 CRPro (filled squares), TspB1628 CR (filled triangles), TspB1548

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Identification of a phage-encoded immunoglobulin-binding protein from invasive Neisseria meningitidis

    doi: 10.4049/jimmunol.1301153

    Figure Lengend Snippet: Immunoglobulin-binding activity of recombinant TspB derivatives by ELISA. Panel a. shows IgG-binding activity in whole serum from donor 2 of full length (FL) TspB1628 (filled circles), TspB1628 CRPro (filled squares), TspB1628 CR (filled triangles), TspB1548

    Article Snippet: Fab and Fc fragments of human IgG2 (Millipore) were prepared by incubating the mAb (5 mg/ml) with papain conjugated to agarose beads (Pierce Chemical Company, Rockford, IL) in 25 mM sodium phosphate buffer, pH 7, containing 2 mM cysteine at 37°C with constant mixing for 5 days.

    Techniques: Binding Assay, Activity Assay, Recombinant, Enzyme-linked Immunosorbent Assay

    Effect of bacterial culture conditions on immunoglobulin-binding activity of NmW135 strain A22 and NmB strain H44/76 by flow cytometry. Bacteria were cultured in the indicated media. The rightward shift to higher fluorescence indicates bound human IgG

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Identification of a phage-encoded immunoglobulin-binding protein from invasive Neisseria meningitidis

    doi: 10.4049/jimmunol.1301153

    Figure Lengend Snippet: Effect of bacterial culture conditions on immunoglobulin-binding activity of NmW135 strain A22 and NmB strain H44/76 by flow cytometry. Bacteria were cultured in the indicated media. The rightward shift to higher fluorescence indicates bound human IgG

    Article Snippet: Fab and Fc fragments of human IgG2 (Millipore) were prepared by incubating the mAb (5 mg/ml) with papain conjugated to agarose beads (Pierce Chemical Company, Rockford, IL) in 25 mM sodium phosphate buffer, pH 7, containing 2 mM cysteine at 37°C with constant mixing for 5 days.

    Techniques: Binding Assay, Activity Assay, Flow Cytometry, Cytometry, Cell Culture, Fluorescence

    Biofilm containing TspB, IgG, and DNA enveloping aggregates of NmB cultured in the presence of human serum. Laser scanning confocal micrographs (63x) of an aggregate of NmB strain H44/76 cultured in CDM+HuS and mouse anti-TspB1628 CR. Human IgG is marked

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Identification of a phage-encoded immunoglobulin-binding protein from invasive Neisseria meningitidis

    doi: 10.4049/jimmunol.1301153

    Figure Lengend Snippet: Biofilm containing TspB, IgG, and DNA enveloping aggregates of NmB cultured in the presence of human serum. Laser scanning confocal micrographs (63x) of an aggregate of NmB strain H44/76 cultured in CDM+HuS and mouse anti-TspB1628 CR. Human IgG is marked

    Article Snippet: Fab and Fc fragments of human IgG2 (Millipore) were prepared by incubating the mAb (5 mg/ml) with papain conjugated to agarose beads (Pierce Chemical Company, Rockford, IL) in 25 mM sodium phosphate buffer, pH 7, containing 2 mM cysteine at 37°C with constant mixing for 5 days.

    Techniques: Cell Culture

    Sera from patients with BP do not contain IgG autoantibodies directed against L-332. a. Sera from 13 BP patients scoring above the cut point in the IgG 4 anti-L-332 ELISA (lanes 1-13) immunoblotted either BPAG1 or BPAG2 in HK extracts. None of these samples

    Journal: Journal of the American Academy of Dermatology

    Article Title: IgG Anti-Laminin-332 Autoantibodies Are Present in a Subset of Patients with Mucous Membrane, But Not Bullous, Pemphigoid

    doi: 10.1016/j.jaad.2008.02.035

    Figure Lengend Snippet: Sera from patients with BP do not contain IgG autoantibodies directed against L-332. a. Sera from 13 BP patients scoring above the cut point in the IgG 4 anti-L-332 ELISA (lanes 1-13) immunoblotted either BPAG1 or BPAG2 in HK extracts. None of these samples

    Article Snippet: Affinity purified fluorescein isothiocyanate-conjugated goat F(ab′)2 anti-human IgG (Biosource International, Camarillo, CA), horse radish peroxidase-conjugated goat F(ab′)2 anti-mouse IgG (Biosource), alkaline phosphatase-conjugated goat F(ab′)2 anti-human IgG (Biosource), alkaline phosphatase-conjugated goat F(ab′)2 anti-rabbit IgG (Biosource), and ascites fluids containing mouse monoclonal anti-human IgG1 (clone HP6001), anti-human IgG2 (clone HP6014), anti-human IgG3 (clone HP6050), anti-human IgG4 (clone HP6025) (all from Sigma, St Louis, MO) were used in this study.

    Techniques: Enzyme-linked Immunosorbent Assay

    The IgG 4 anti-L-332 ELISA detects autoantibodies in patients with AECP with great sensitivity and specificity. Scatter plot representation of ELISA results concerning the performance of sera from 32 patients with AECP and 173 controls. All sera were tested

    Journal: Journal of the American Academy of Dermatology

    Article Title: IgG Anti-Laminin-332 Autoantibodies Are Present in a Subset of Patients with Mucous Membrane, But Not Bullous, Pemphigoid

    doi: 10.1016/j.jaad.2008.02.035

    Figure Lengend Snippet: The IgG 4 anti-L-332 ELISA detects autoantibodies in patients with AECP with great sensitivity and specificity. Scatter plot representation of ELISA results concerning the performance of sera from 32 patients with AECP and 173 controls. All sera were tested

    Article Snippet: Affinity purified fluorescein isothiocyanate-conjugated goat F(ab′)2 anti-human IgG (Biosource International, Camarillo, CA), horse radish peroxidase-conjugated goat F(ab′)2 anti-mouse IgG (Biosource), alkaline phosphatase-conjugated goat F(ab′)2 anti-human IgG (Biosource), alkaline phosphatase-conjugated goat F(ab′)2 anti-rabbit IgG (Biosource), and ascites fluids containing mouse monoclonal anti-human IgG1 (clone HP6001), anti-human IgG2 (clone HP6014), anti-human IgG3 (clone HP6050), anti-human IgG4 (clone HP6025) (all from Sigma, St Louis, MO) were used in this study.

    Techniques: Enzyme-linked Immunosorbent Assay

    Antibody responses against LukM. IgG1 ( a , b , c ) and IgG2 ( d , e , f ) antibodies specific for LukM measured in serum ( a , d ), colostrum ( b , e ) and milk ( c , f ). S/P = Sample to positive ratio. + = P

    Journal: BMC Veterinary Research

    Article Title: Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk

    doi: 10.1186/s12917-018-1765-9

    Figure Lengend Snippet: Antibody responses against LukM. IgG1 ( a , b , c ) and IgG2 ( d , e , f ) antibodies specific for LukM measured in serum ( a , d ), colostrum ( b , e ) and milk ( c , f ). S/P = Sample to positive ratio. + = P

    Article Snippet: Monoclonal mouse anti-bovine IgG1 and IgG2 (Prionics Lelystad B.V., Life Technologies, Thermo Fisher Scientific) were used as secondary antibodies.

    Techniques:

    Antibody responses against EfB. IgG1 ( a , b , c ) and IgG2 ( d , e , f ) antibodies specific for EfB measured in serum ( a , d ), colostrum ( b , e ) and milk ( c , f ). S/P = Sample to positive ratio. + = P

    Journal: BMC Veterinary Research

    Article Title: Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk

    doi: 10.1186/s12917-018-1765-9

    Figure Lengend Snippet: Antibody responses against EfB. IgG1 ( a , b , c ) and IgG2 ( d , e , f ) antibodies specific for EfB measured in serum ( a , d ), colostrum ( b , e ) and milk ( c , f ). S/P = Sample to positive ratio. + = P

    Article Snippet: Monoclonal mouse anti-bovine IgG1 and IgG2 (Prionics Lelystad B.V., Life Technologies, Thermo Fisher Scientific) were used as secondary antibodies.

    Techniques:

    Dynamics of Staphylococcus aureus specific antibodies following natural exposure. IgG1 ( a , c , e ) and IgG2 ( b , d , f ) antibodies specific for whole SA bacterium ( a , b ), LukM ( c , d ) and EfB ( e , f ) measured in colostrum of dams and in serum of calves from before colostrum uptake until week 29. S/P = Sample to positive ratio

    Journal: BMC Veterinary Research

    Article Title: Immunization of young heifers with staphylococcal immune evasion proteins before natural exposure to Staphylococcus aureus induces a humoral immune response in serum and milk

    doi: 10.1186/s12917-018-1765-9

    Figure Lengend Snippet: Dynamics of Staphylococcus aureus specific antibodies following natural exposure. IgG1 ( a , c , e ) and IgG2 ( b , d , f ) antibodies specific for whole SA bacterium ( a , b ), LukM ( c , d ) and EfB ( e , f ) measured in colostrum of dams and in serum of calves from before colostrum uptake until week 29. S/P = Sample to positive ratio

    Article Snippet: Monoclonal mouse anti-bovine IgG1 and IgG2 (Prionics Lelystad B.V., Life Technologies, Thermo Fisher Scientific) were used as secondary antibodies.

    Techniques:

    Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and α-MEM medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p

    Journal: Scientific Reports

    Article Title: Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice

    doi: 10.1038/s41598-018-30076-7

    Figure Lengend Snippet: Humoral and cellular immune responses induced by different schemes of vaccination with Δ Likh1 . Humoral immune response was evaluated by conventional ELISA measurement of serum levels of different isotypes of Li WT SLA-specific antibodies (IgG total , IgG 1 and IgG 2a ). ( a ) Seroconversion of mice 20 days post-immunization with Δ Likh1 . ( b ) IgG2a:IgG1 ratio between IV and SC prime-boosted groups. ( c – h ) Levels of cytokines in pg/mL in the supernatant of Li WT SLA-stimulated splenocytes. The concentration of Li WT SLA in the experiments was 25 μg/mL and splenocytes were stimulated for 72 h. CpG ODN at 5 μg/mL was used as a positive control of a Th1-response and α-MEM medium with 20% of FBS was used as negative control. Cytokines were measured by flow cytometry with the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) following manufacturer instructions. The acquisition and data analyses were made in BD FACSVerse Instrument and FCAP Array Software v3. The detection limit of IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A and IL-10 cytokines were respectively 0.1; 0.03; 1.4; 0.5; 0.9; 0.8 and 16.8 pg/mL as well as cytokines secreted by Li WT SLA-stimulated splenocytes from immunized mice. Data are shown as means ± SE. Statistical analyses used One-way-ANOVA followed by Bonferroni’s multiple comparison test. *** p

    Article Snippet: Peroxidase-conjugated rabbit anti-mouse IgG Total, IgG1 and IgG2 antibodies were added at a 1:5000 dilution for 1 h. The reaction was performed by addition of o-phenylenediamine and H2 O2 (SigmaAldrich, St. Louis, MI, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Concentration Assay, Positive Control, Negative Control, Flow Cytometry, Cytometry, Crocin Bleaching Assay, FCAP Assay, Software

    IgG binding characteristics of FcγR1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: IgG binding characteristics of FcγR1

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: Binding Assay

    Summary of affinities of rhesus macaque IgG samples across MM FcγR as determined by SPR

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: Summary of affinities of rhesus macaque IgG samples across MM FcγR as determined by SPR

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: SPR Assay

    IgG binding characteristics of low affinity MM FcγR

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IgG Binding Characteristics of Rhesus Macaque FcγR

    doi: 10.4049/jimmunol.1502252

    Figure Lengend Snippet: IgG binding characteristics of low affinity MM FcγR

    Article Snippet: Human polyclonal IgG1, IgG2, and IgG3 subclasses as well as human myeloma IgG1, IgG2, and IgG4 subclasses (monoclonal) were obtained from Athens Research Corporation.

    Techniques: Binding Assay

    sCD4 and CD4-IgG2 induce the shedding of Bx08 gp120. Purified virus was incubated with PBS (mock-treated control, left panel) or 50-μg/ml sCD4 (central panel) or CD4-IgG2 (right panel) for 1 h at 37°C, and viral particles were further purified by parallel gel filtration columns. Dosage determinations for the viral proteins p24 (empty squares) and gp120 (black triangles) in the collected fractions were performed as indicated in Materials and Methods.

    Journal: Journal of Virology

    Article Title: Polyclonal Immunoglobulin G from Patients Neutralizes Human Immunodeficiency Virus Type 1 Primary Isolates by Binding Free Virions, but without Interfering with an Initial CD4-Independent Attachment of the Virus to Primary Blood Mononuclear Cells

    doi: 10.1128/JVI.77.21.11385-11397.2003

    Figure Lengend Snippet: sCD4 and CD4-IgG2 induce the shedding of Bx08 gp120. Purified virus was incubated with PBS (mock-treated control, left panel) or 50-μg/ml sCD4 (central panel) or CD4-IgG2 (right panel) for 1 h at 37°C, and viral particles were further purified by parallel gel filtration columns. Dosage determinations for the viral proteins p24 (empty squares) and gp120 (black triangles) in the collected fractions were performed as indicated in Materials and Methods.

    Article Snippet: The CD4-IgG2 fusion protein , was provided by W. Olson and Progenics Pharmaceuticals.

    Techniques: Purification, Incubation, Filtration

    Attachment and neutralization of HIV-1 PIs in the presence of anti-CD4 MAbs, sCD4, and CD4-IgG2. Bx08 (A and B) and Bx17 (C) neutralization (diamonds) and attachment to PBMC (triangles) were measured under conditions where the PBMC were preincubated with anti-CD4 MAbs (A and C, left) or where the virus was preincubated with sCD4 or CD4-IgG2 (B and C, right). Each value corresponds to the mean and standard deviation of the results of at least two independent experiments, except for CD4-IgG2 with Bx17, which was performed only once. Cultures of samples in the presence of 10 −6 M AZT (dashed line, open diamonds) give background p24 values for neutralization.

    Journal: Journal of Virology

    Article Title: Polyclonal Immunoglobulin G from Patients Neutralizes Human Immunodeficiency Virus Type 1 Primary Isolates by Binding Free Virions, but without Interfering with an Initial CD4-Independent Attachment of the Virus to Primary Blood Mononuclear Cells

    doi: 10.1128/JVI.77.21.11385-11397.2003

    Figure Lengend Snippet: Attachment and neutralization of HIV-1 PIs in the presence of anti-CD4 MAbs, sCD4, and CD4-IgG2. Bx08 (A and B) and Bx17 (C) neutralization (diamonds) and attachment to PBMC (triangles) were measured under conditions where the PBMC were preincubated with anti-CD4 MAbs (A and C, left) or where the virus was preincubated with sCD4 or CD4-IgG2 (B and C, right). Each value corresponds to the mean and standard deviation of the results of at least two independent experiments, except for CD4-IgG2 with Bx17, which was performed only once. Cultures of samples in the presence of 10 −6 M AZT (dashed line, open diamonds) give background p24 values for neutralization.

    Article Snippet: The CD4-IgG2 fusion protein , was provided by W. Olson and Progenics Pharmaceuticals.

    Techniques: Neutralization, Standard Deviation