Journal: PLoS ONE
Article Title: Mapping the Binding between the Tetraspanin Molecule (Sjc23) ofSchistosoma japonicum and Human Non-Immune IgG
Figure Lengend Snippet: Binding of Sjc23-LED with immunoglobulins in pull-down assay. A Human IgG (lane 1), IgA (lane 4), IgE (lane 6), and IgM (lane 8) were incubated with Sjc23-LED bound Sepharose resin, after extensive washing, the proteins were resolved in SDS-PAGE and blotted to nylon film. The immunoglobulins that bound to Sjc23-LED was detected by using mAbs specific for human antibodies (γ-chain, α-chain,ε-chain or µ-chain specific). Only human IgG could specifically bind to Sjc23-LED, whereas the other antibody types did not. GST did not bind human IgG (lane 2). Lanes 3, 5, 7 and 9 were corresponding antibodies as controls for detection. B Porcine and bovine IgG was respectively incubated with Sjc23-LED bound Sepharose resin and only porcine IgG was marginally precipitated (lane 1, the band was indicated with an asterisk). Lane 2 and 4 were controls of the corresponding IgGs.
Article Snippet: The three proteins, Sjc23-LED, GST and TSP-2, were incubated respectively with purified human IgG, IgM, IgA (Sigma, CA, USA) and IgE (Abcam, Cambridge, UK).
Techniques: Binding Assay, Pull Down Assay, Incubation, SDS Page