Journal: Clinical and Translational Allergy

Article Title: Deep analysis of immune response and metabolic signature in children with food protein induced enterocolitis to cow’s milk

doi: 10.1186/s13601-018-0224-9

Figure Lengend Snippet: Analysis of proliferative T cells in CMA patients after allergen reactivation. PBMC from IgE-CMA ( a ) or FPIES-CMA ( b ) patients were labelled with CFSE and then cultured for 6 days with PBS or allergens purified from cow’s milk. Cells were then recovered and labelled with anti-human CD4. Among SSC-FSC gated cells, single cells were selected and analyzed for CD4 expression and CFSE signal. Proliferative T cells are defined as CD4 + CFSE low cells within a selected population (red square). Proliferative cells after reactivation with PBS, BLG, caseins or \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α -lact are shown

Article Snippet: Standard curves obtained with anti-human IgE [ ] or anti-human IgG (F(ab′)2 Fragment specific, Pierce ® , Thermo Scientific, Rockford, USA) coated plates and standard human IgE (World Health Organization; concentrations ranging from 10 to 0.08 IU/mL) or commercial isotype standard IgGs (all from AbD-Serotec, Bio-Rad, concentrations ranging from 1 μg/mL to 50 ng/mL) were used as reference to quantify antibody concentrations.

Techniques: Cell Culture, Purification, Expressing