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  • 96
    Millipore ige
    PMX-53 and CST do not activate murine peritoneal and bone marrow-derived mast cells. A, murine bone marrow-derived and peritoneal mast cells were incubated with <t>DNP-specific</t> mouse <t>IgE</t> (1 μg/ml, 16 h). Cells were exposed to buffer (control), 1
    Ige, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore dnp immunoglobulin e dnp ige
    Inhibitory effects of KIOM-MA128 on the production of allergic mediators. <t>IgE-sensitized</t> RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p
    Dnp Immunoglobulin E Dnp Ige, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Quest Diagnostics immunocap immunoglobulin e
    Inhibitory effects of KIOM-MA128 on the production of allergic mediators. <t>IgE-sensitized</t> RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p
    Immunocap Immunoglobulin E, supplied by Quest Diagnostics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ige  (Bio-Rad)
    92
    Bio-Rad ige
    TSLP shifts the immune response to gp140 to Th2 Mice were immunised three times i.n. with 10 μg gp140 alone or with 5 μg TSLP or CT. (A) Anti-gp140 IgG 1 and IgG 2a and (B) <t>IgE</t> were measured in serum by ELISA. (C) Splenocytes were stimulated with a CN54 gp140 peptide pool and specific cytokine production was measured in CD4 + T cells by flow cytometry. (D) Mice were immunised twice at 2 week intervals, followed by three daily i.n. challenges with 20 μg gp140 in 100 μL and were culled 3 days later. (E) gp140-specific IgE was measured in sera. (F) Cells were collected from bronchoalveolar lavage (BAL) and counted and (G) differential cell counts performed. Representative PAS-stained sections of lungs from (H) PBS, (I) gp140, (J) gp140:TSLP, (K) gp140:CT, (L) IP gp140:alum. Data are shown as mean of n =5 mice+SD, experiment performed once. * p
    Ige, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam immunoglobulin ige human elisa kit
    TSLP shifts the immune response to gp140 to Th2 Mice were immunised three times i.n. with 10 μg gp140 alone or with 5 μg TSLP or CT. (A) Anti-gp140 IgG 1 and IgG 2a and (B) <t>IgE</t> were measured in serum by ELISA. (C) Splenocytes were stimulated with a CN54 gp140 peptide pool and specific cytokine production was measured in CD4 + T cells by flow cytometry. (D) Mice were immunised twice at 2 week intervals, followed by three daily i.n. challenges with 20 μg gp140 in 100 μL and were culled 3 days later. (E) gp140-specific IgE was measured in sera. (F) Cells were collected from bronchoalveolar lavage (BAL) and counted and (G) differential cell counts performed. Representative PAS-stained sections of lungs from (H) PBS, (I) gp140, (J) gp140:TSLP, (K) gp140:CT, (L) IP gp140:alum. Data are shown as mean of n =5 mice+SD, experiment performed once. * p
    Immunoglobulin Ige Human Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Bethyl immunoglobulin e ige concentrations
    Effect of egg white treatment on mouse immunoglobulin production. Blood samples were collected from EW (0, 10, 50, and 100 mg/d)-treated mice at 4 wk. The samples were incubated with anti-mouse IgG (A) and <t>IgE</t> (B) antibodies. A horseradish peroxidase-conjugated secondary antibody was used to detect the absorbance of each Ig. p -values were calculated between 0 mg/d (control) and each EW treatment at 5 wk (* p
    Immunoglobulin E Ige Concentrations, supplied by Bethyl, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    College of American Pathologists serum immunoglobulin e
    Effect of egg white treatment on mouse immunoglobulin production. Blood samples were collected from EW (0, 10, 50, and 100 mg/d)-treated mice at 4 wk. The samples were incubated with anti-mouse IgG (A) and <t>IgE</t> (B) antibodies. A horseradish peroxidase-conjugated secondary antibody was used to detect the absorbance of each Ig. p -values were calculated between 0 mg/d (control) and each EW treatment at 5 wk (* p
    Serum Immunoglobulin E, supplied by College of American Pathologists, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher immunoglobulin e sige
    Effect of egg white treatment on mouse immunoglobulin production. Blood samples were collected from EW (0, 10, 50, and 100 mg/d)-treated mice at 4 wk. The samples were incubated with anti-mouse IgG (A) and <t>IgE</t> (B) antibodies. A horseradish peroxidase-conjugated secondary antibody was used to detect the absorbance of each Ig. p -values were calculated between 0 mg/d (control) and each EW treatment at 5 wk (* p
    Immunoglobulin E Sige, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Journal of Biological Chemistry immunoglobulin e
    Effect of egg white treatment on mouse immunoglobulin production. Blood samples were collected from EW (0, 10, 50, and 100 mg/d)-treated mice at 4 wk. The samples were incubated with anti-mouse IgG (A) and <t>IgE</t> (B) antibodies. A horseradish peroxidase-conjugated secondary antibody was used to detect the absorbance of each Ig. p -values were calculated between 0 mg/d (control) and each EW treatment at 5 wk (* p
    Immunoglobulin E, supplied by Journal of Biological Chemistry, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Targeted Cell Therapies LLC immunoglobulin e ige targeted therapies
    Effect of egg white treatment on mouse immunoglobulin production. Blood samples were collected from EW (0, 10, 50, and 100 mg/d)-treated mice at 4 wk. The samples were incubated with anti-mouse IgG (A) and <t>IgE</t> (B) antibodies. A horseradish peroxidase-conjugated secondary antibody was used to detect the absorbance of each Ig. p -values were calculated between 0 mg/d (control) and each EW treatment at 5 wk (* p
    Immunoglobulin E Ige Targeted Therapies, supplied by Targeted Cell Therapies LLC, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Becton Dickinson ige
    H. polygyrus infection promotes a strong type 2 immune response. Real time qPCR analysis shows a clear suppression in the T-bet and RORγt mRNAs expression ( a and b ) and increase in the expression of GATA3 and Foxp3 mRNAs ( c and d ) in the MLN cells from HFD-fed H. polygyrus -infected mice. H. polygyrus -infected obese mice showed decreases in IFN-γ and IL-17 secretions in the supernatant of cultured MLN cells ( e and f ). H. polygyrus -infected obese mice had higher production of cytokines IL-4 and IL-10 in MLN ( g and h ). H. polygyrus -infected obese mice had increased levels of serum <t>IgE</t> ( i ) and <t>IgG1</t> ( j) and a decreased IgG2a level ( k ). (n = 5–6; * p
    Ige, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 1480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam human immunoglobulin e
    Lateral flow assay with aptamer-phage for <t>IgE</t> detection. Various concentrations of IgE (volume: 10 μL) were detected using anti-IgE antibodies at the test line and IgE aptamer-HRP-phage reporters. Control line consisted of anti-M13 antibodies.
    Human Immunoglobulin E, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Specific Diagnostics immunoglobulin e
    Fc͛RI-bound <t>IgE</t> on effector cells. Light gray area: total bound IgE/cell (number of Fc͛RI occupied by IgE with population-based distribution) on basophilic leukocytes. Dark gray area: specific IgE/cell required for half-maximal cell activation (intrinsic sensitivity of basophils with population-based distribution). The distribution of both variables is approximately normal and can differ significantly; evidently, a fraction (ca. 1%) of bound total IgE is sufficient for half-maximal allergen specific activation. For this reason, the ratio of specific to total IgE is interesting in terms of interpretation. Inset top left: individual mediator release as a function of cell-bound specific IgE; basis for the population-based normal distributions illustrated in the lower part of the figure.
    Immunoglobulin E, supplied by Specific Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Athens Research immunoglobulin e
    While binding-induced changes in the flexibility of folded aptamers sometimes support E-AB signaling, the magnitude of this effect varies dramatically from aptamer-to-aptamer. (Left) For example, even though <t>IgE</t> is a rather larger target than thrombin, the binding of IgE (at a saturating concentration of 200 nM) to its (folded) aptamer produces only a small change in E-AB current. The magnitude of this change is similar to that observed for buffer blanks, which vary slightly due to minor sensor degradation (at the ca. 1% level over this timescale). (Right) Quartz crystal microbalance experiments confirm that the immobilized aptamer retains its ability to bind target and thus demonstrate that poor signaling, rather than poor binding, underlies the limited E-AB signaling.
    Immunoglobulin E, supplied by Athens Research, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher ige
    No cross-reactivity between bGST and HDM-GST. (A) IgG1-reactivity of sera (n = 3) collected from bGST-immunized mice at day 0 and day 59 to <t>BPE</t> and HDME. (B) <t>IgE-reactivity</t> of seven Der p 8-sensitized HDM-allergic patients to bGST and HDME. NHS, non-allergic control sera; O.D. optical density; dotted and dashed lines indicate the cut-off for positive IgE-reactivity.
    Ige, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Mediatech immunoglobulin e
    (a) Concentrations of total <t>immunoglobulin</t> E (IgE; ng/ml) and (b) levels of insulin-specific IgG1 in the plasma of mice that were implanted intraperitoneally with five female or male adult worms ( n = 12) or sham-treated controls ( n = 11). Significant differences
    Immunoglobulin E, supplied by Mediatech, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Athens Research human myeloma immunoglobulin e
    Images and linescan plots recorded at various time points during an μFFE gradient where a constant concentration of fluorescently labeled aptamer for <t>IgE</t> was titrated with an increasing concentration of IgE. Peaks from left to right were identified as the unbound aptamer, the aptamer-IgE complex, and the internal standard (rhodamine 110). The anode is at the left side of the images.
    Human Myeloma Immunoglobulin E, supplied by Athens Research, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Immundiagnostik AG immunoglobulin e elisa kit
    Images and linescan plots recorded at various time points during an μFFE gradient where a constant concentration of fluorescently labeled aptamer for <t>IgE</t> was titrated with an increasing concentration of IgE. Peaks from left to right were identified as the unbound aptamer, the aptamer-IgE complex, and the internal standard (rhodamine 110). The anode is at the left side of the images.
    Immunoglobulin E Elisa Kit, supplied by Immundiagnostik AG, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Targeted Therapeutic Solutions LLC immunoglobulin e ige targeted therapeutic responses
    Images and linescan plots recorded at various time points during an μFFE gradient where a constant concentration of fluorescently labeled aptamer for <t>IgE</t> was titrated with an increasing concentration of IgE. Peaks from left to right were identified as the unbound aptamer, the aptamer-IgE complex, and the internal standard (rhodamine 110). The anode is at the left side of the images.
    Immunoglobulin E Ige Targeted Therapeutic Responses, supplied by Targeted Therapeutic Solutions LLC, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Mimotopes immunoglobulin e ige allergen binding
    Peptide ELISA demonstrating the binding of Met e 1-specific <t>IgE</t> to the mimotopes. Mimotope KGIPNTKAP of Der p 1 and an IgE-binding epitope of Met e 1 (a.a. 251-270) were included as negative and positive controls, respectively. Met e 1-specific IgE could exclusively recognize the selected mimotopes from clusters 1–6, but not the irrelevant mimotope of Der p 1 ( P
    Immunoglobulin E Ige Allergen Binding, supplied by Mimotopes, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    NCGS non immunoglobulin e non ige wheat allergy
    Peptide ELISA demonstrating the binding of Met e 1-specific <t>IgE</t> to the mimotopes. Mimotope KGIPNTKAP of Der p 1 and an IgE-binding epitope of Met e 1 (a.a. 251-270) were included as negative and positive controls, respectively. Met e 1-specific IgE could exclusively recognize the selected mimotopes from clusters 1–6, but not the irrelevant mimotope of Der p 1 ( P
    Non Immunoglobulin E Non Ige Wheat Allergy, supplied by NCGS, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore human myeloma ige
    Levels of <t>IgE</t> and <t>IgG4</t> to Schistosoma mansoni tropomyosin (Tpm)II isoforms. Levels of IgE (A) and IgG4 (B) to TpmII isoforms were measured in a population of infected boys and men ( n = 228) from an area endemic for S. mansoni , both before and 7 weeks after treatment with Praziquantel. Shown are the levels (geometric mean ± 95% confidence interval) for those with a detectable response. The prevalence (%) of each response (before treatment) in the cohort is indicated.
    Human Myeloma Ige, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ige  (Abcam)
    93
    Abcam ige
    Binding of Sjc23-LED with immunoglobulins in pull-down assay. A Human <t>IgG</t> (lane 1), IgA (lane 4), <t>IgE</t> (lane 6), and IgM (lane 8) were incubated with Sjc23-LED bound Sepharose resin, after extensive washing, the proteins were resolved in SDS-PAGE and blotted to nylon film. The immunoglobulins that bound to Sjc23-LED was detected by using mAbs specific for human antibodies (γ-chain, α-chain,ε-chain or µ-chain specific). Only human IgG could specifically bind to Sjc23-LED, whereas the other antibody types did not. GST did not bind human IgG (lane 2). Lanes 3, 5, 7 and 9 were corresponding antibodies as controls for detection. B Porcine and bovine IgG was respectively incubated with Sjc23-LED bound Sepharose resin and only porcine IgG was marginally precipitated (lane 1, the band was indicated with an asterisk). Lane 2 and 4 were controls of the corresponding IgGs.
    Ige, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ige  (Bethyl)
    93
    Bethyl ige
    BV reduces OVA-induced mast cells, serum <t>IgE</t> and pro-inflammatory cytokine expression. (A) Giemsa staining: Representative images of histological analysis, presenting increased infiltration and degranulation of the mast cells. However, it was reduced under BV treatment. Magnification, ×400; scale bars, 50 µm. (B) Infiltration and degranulation were evaluated by assessing the number of mast cells from at least 3 random fields per section at 400-fold magnification. (C) The <t>ELISA</t> results demonstrated that BV inhibited the increased levels of serum-IgE induced by OVA. In addition, BV inhibited the increased expression of inflammatory cytokines such as (D) TNF-α and (E) TSLP induced by OVA. The results are expressed as the mean ± standard error of the mean of 3 independent determinations. *P
    Ige, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    EUROIMMUN total immunoglobulin e
    BV reduces OVA-induced mast cells, serum <t>IgE</t> and pro-inflammatory cytokine expression. (A) Giemsa staining: Representative images of histological analysis, presenting increased infiltration and degranulation of the mast cells. However, it was reduced under BV treatment. Magnification, ×400; scale bars, 50 µm. (B) Infiltration and degranulation were evaluated by assessing the number of mast cells from at least 3 random fields per section at 400-fold magnification. (C) The <t>ELISA</t> results demonstrated that BV inhibited the increased levels of serum-IgE induced by OVA. In addition, BV inhibited the increased expression of inflammatory cytokines such as (D) TNF-α and (E) TSLP induced by OVA. The results are expressed as the mean ± standard error of the mean of 3 independent determinations. *P
    Total Immunoglobulin E, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PMX-53 and CST do not activate murine peritoneal and bone marrow-derived mast cells. A, murine bone marrow-derived and peritoneal mast cells were incubated with DNP-specific mouse IgE (1 μg/ml, 16 h). Cells were exposed to buffer (control), 1

    Journal: Molecular Pharmacology

    Article Title: PMX-53 as a Dual CD88 Antagonist and an Agonist for Mas-Related Gene 2 (MrgX2) in Human Mast Cells

    doi: 10.1124/mol.111.071472

    Figure Lengend Snippet: PMX-53 and CST do not activate murine peritoneal and bone marrow-derived mast cells. A, murine bone marrow-derived and peritoneal mast cells were incubated with DNP-specific mouse IgE (1 μg/ml, 16 h). Cells were exposed to buffer (control), 1

    Article Snippet: Monoclonal anti-DNP specific IgE and anti-human IgE were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Derivative Assay, Incubation

    PMX-53 uses MrgX2 to induce mast cell degranulation. RBL-2H3 cells stably expressing MrgX1 (A) or MrgX2 (B) were incubated with anti-DNP specific IgE (1 μg/ml, 16 h) and stimulated with the indicated peptides (1 μM), substance P (SP; 1

    Journal: Molecular Pharmacology

    Article Title: PMX-53 as a Dual CD88 Antagonist and an Agonist for Mas-Related Gene 2 (MrgX2) in Human Mast Cells

    doi: 10.1124/mol.111.071472

    Figure Lengend Snippet: PMX-53 uses MrgX2 to induce mast cell degranulation. RBL-2H3 cells stably expressing MrgX1 (A) or MrgX2 (B) were incubated with anti-DNP specific IgE (1 μg/ml, 16 h) and stimulated with the indicated peptides (1 μM), substance P (SP; 1

    Article Snippet: Monoclonal anti-DNP specific IgE and anti-human IgE were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Stable Transfection, Expressing, Incubation

    Inhibitory effects of KIOM-MA128 on the production of allergic mediators. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on the production of allergic mediators. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h prior to antigen treatment. The levels of TNF-α, IL-4 and IL-6 were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques:

    Inhibitory effects of KIOM-MA128 on PGD 2 production and the activation of the arachidonate cascade. RBL-2H3 mast cells were seeded on a 6-well plate (5 × 10 5 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. The amounts of PGD 2 were determined as described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. The cells were washed with 1× DPBS and lysed with cell lysis buffer. The levels of p-cPLA 2 , COX-2 and α-tubulin were determined using the procedure described in the Materials and Methods section. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on PGD 2 production and the activation of the arachidonate cascade. RBL-2H3 mast cells were seeded on a 6-well plate (5 × 10 5 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. The amounts of PGD 2 were determined as described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. The cells were washed with 1× DPBS and lysed with cell lysis buffer. The levels of p-cPLA 2 , COX-2 and α-tubulin were determined using the procedure described in the Materials and Methods section. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Activation Assay, Incubation, Lysis

    Inhibitory effect of KIOM-MA128 on Ag/IgE-induced passive cutaneous anaphylaxis in mice. IgE-sensitized mice were orally administered KIOM-MA128 (0–100 mg/kg) for 1 h and then intravenously injected with 100 μg DNP-HSA containing 0.5% Evans blue. After 30 min, the mice were euthanized, and then both ears were excised. The extravasated dye in the ears was analyzed using the procedure described in the Materials and Methods section. The data are listed as the mean ± SEM values from eight determinations. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effect of KIOM-MA128 on Ag/IgE-induced passive cutaneous anaphylaxis in mice. IgE-sensitized mice were orally administered KIOM-MA128 (0–100 mg/kg) for 1 h and then intravenously injected with 100 μg DNP-HSA containing 0.5% Evans blue. After 30 min, the mice were euthanized, and then both ears were excised. The extravasated dye in the ears was analyzed using the procedure described in the Materials and Methods section. The data are listed as the mean ± SEM values from eight determinations. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Mouse Assay, Injection

    Inhibitory effects of KIOM-MA128 on the activation of the FcεRI cascade. IgE-sensitized RBL-2H3 cells were treated with KIOM-MA128 for 1 h and then stimulated with DNP-Ag for 10 min. The cells were washed with 1 × DPBS and lysed with cell lysis buffer. The levels of p-SYK, p-LYN, p-FYN, p-ERK, p-p38, p-JNK, p-PLCγ1, p-PKCδ and α-tubulin were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. ( A ) p-SYK, p-LYN and p-FYN; ( B ) p-ERK, p-p38 and p-JNK; ( C ) p-PLCγ1 and p-PKCδ. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on the activation of the FcεRI cascade. IgE-sensitized RBL-2H3 cells were treated with KIOM-MA128 for 1 h and then stimulated with DNP-Ag for 10 min. The cells were washed with 1 × DPBS and lysed with cell lysis buffer. The levels of p-SYK, p-LYN, p-FYN, p-ERK, p-p38, p-JNK, p-PLCγ1, p-PKCδ and α-tubulin were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. ( A ) p-SYK, p-LYN and p-FYN; ( B ) p-ERK, p-p38 and p-JNK; ( C ) p-PLCγ1 and p-PKCδ. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Activation Assay, Lysis

    Inhibitory effects of KIOM-MA128 on degranulation and histamine release. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h before antigen challenge. β-hexosaminidase activity and the histamine concentrations were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on degranulation and histamine release. IgE-sensitized RBL-2H3 mast cells were treated with KIOM-MA128 for 1 h before antigen challenge. β-hexosaminidase activity and the histamine concentrations were determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. # p

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Activity Assay

    Effect of KIOM-MA128 on cell viability in IgE/Ag-activated RBL-2H3 mast cells. RBL-2H3 mast cells were seeded on a 96-well plate (1 × 10 4 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. Cell viability was determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments.

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Effect of KIOM-MA128 on cell viability in IgE/Ag-activated RBL-2H3 mast cells. RBL-2H3 mast cells were seeded on a 96-well plate (1 × 10 4 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. Cell viability was determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments.

    Article Snippet: 4-Nitrophenyl-N -acetyl-β-d -glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).

    Techniques: Incubation

    TSLP shifts the immune response to gp140 to Th2 Mice were immunised three times i.n. with 10 μg gp140 alone or with 5 μg TSLP or CT. (A) Anti-gp140 IgG 1 and IgG 2a and (B) IgE were measured in serum by ELISA. (C) Splenocytes were stimulated with a CN54 gp140 peptide pool and specific cytokine production was measured in CD4 + T cells by flow cytometry. (D) Mice were immunised twice at 2 week intervals, followed by three daily i.n. challenges with 20 μg gp140 in 100 μL and were culled 3 days later. (E) gp140-specific IgE was measured in sera. (F) Cells were collected from bronchoalveolar lavage (BAL) and counted and (G) differential cell counts performed. Representative PAS-stained sections of lungs from (H) PBS, (I) gp140, (J) gp140:TSLP, (K) gp140:CT, (L) IP gp140:alum. Data are shown as mean of n =5 mice+SD, experiment performed once. * p

    Journal: European Journal of Immunology

    Article Title: Thymic stromal lymphopoietin (TSLP) acts as a potent mucosal adjuvant for HIV-1 gp140 vaccination in mice

    doi: 10.1002/eji.201141787

    Figure Lengend Snippet: TSLP shifts the immune response to gp140 to Th2 Mice were immunised three times i.n. with 10 μg gp140 alone or with 5 μg TSLP or CT. (A) Anti-gp140 IgG 1 and IgG 2a and (B) IgE were measured in serum by ELISA. (C) Splenocytes were stimulated with a CN54 gp140 peptide pool and specific cytokine production was measured in CD4 + T cells by flow cytometry. (D) Mice were immunised twice at 2 week intervals, followed by three daily i.n. challenges with 20 μg gp140 in 100 μL and were culled 3 days later. (E) gp140-specific IgE was measured in sera. (F) Cells were collected from bronchoalveolar lavage (BAL) and counted and (G) differential cell counts performed. Representative PAS-stained sections of lungs from (H) PBS, (I) gp140, (J) gp140:TSLP, (K) gp140:CT, (L) IP gp140:alum. Data are shown as mean of n =5 mice+SD, experiment performed once. * p

    Article Snippet: Bound IgA and IgG was detected by incubation for 1 h at 37°C with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, IgE, IgG1 , IgG2a (AbD Serotec, UK) or biotin-conjugated goat anti-mouse IgA and IgE (AbD Serotec, UK).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry, Staining

    Effect of egg white treatment on mouse immunoglobulin production. Blood samples were collected from EW (0, 10, 50, and 100 mg/d)-treated mice at 4 wk. The samples were incubated with anti-mouse IgG (A) and IgE (B) antibodies. A horseradish peroxidase-conjugated secondary antibody was used to detect the absorbance of each Ig. p -values were calculated between 0 mg/d (control) and each EW treatment at 5 wk (* p

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Effects of Egg White Consumption on Allergy, Immune Modulation, and Blood Cholesterol Levels in BALB/c Mice

    doi: 10.5851/kosfa.2014.34.5.630

    Figure Lengend Snippet: Effect of egg white treatment on mouse immunoglobulin production. Blood samples were collected from EW (0, 10, 50, and 100 mg/d)-treated mice at 4 wk. The samples were incubated with anti-mouse IgG (A) and IgE (B) antibodies. A horseradish peroxidase-conjugated secondary antibody was used to detect the absorbance of each Ig. p -values were calculated between 0 mg/d (control) and each EW treatment at 5 wk (* p

    Article Snippet: Analysis of immunoglobulin The immunoglobulin G (IgG) and immunoglobulin E (IgE) concentrations in the whole blood samples were measured by a mouse IgG and IgE enzyme-linked immunosorbent assay using a quantitation kit (Bethyl Laboratory Inc., USA) in accordance with the manufacturer’s instructions.

    Techniques: Mouse Assay, Incubation

    H. polygyrus infection promotes a strong type 2 immune response. Real time qPCR analysis shows a clear suppression in the T-bet and RORγt mRNAs expression ( a and b ) and increase in the expression of GATA3 and Foxp3 mRNAs ( c and d ) in the MLN cells from HFD-fed H. polygyrus -infected mice. H. polygyrus -infected obese mice showed decreases in IFN-γ and IL-17 secretions in the supernatant of cultured MLN cells ( e and f ). H. polygyrus -infected obese mice had higher production of cytokines IL-4 and IL-10 in MLN ( g and h ). H. polygyrus -infected obese mice had increased levels of serum IgE ( i ) and IgG1 ( j) and a decreased IgG2a level ( k ). (n = 5–6; * p

    Journal: Scientific Reports

    Article Title: Helminth infection protects against high fat diet-induced obesity via induction of alternatively activated macrophages

    doi: 10.1038/s41598-018-22920-7

    Figure Lengend Snippet: H. polygyrus infection promotes a strong type 2 immune response. Real time qPCR analysis shows a clear suppression in the T-bet and RORγt mRNAs expression ( a and b ) and increase in the expression of GATA3 and Foxp3 mRNAs ( c and d ) in the MLN cells from HFD-fed H. polygyrus -infected mice. H. polygyrus -infected obese mice showed decreases in IFN-γ and IL-17 secretions in the supernatant of cultured MLN cells ( e and f ). H. polygyrus -infected obese mice had higher production of cytokines IL-4 and IL-10 in MLN ( g and h ). H. polygyrus -infected obese mice had increased levels of serum IgE ( i ) and IgG1 ( j) and a decreased IgG2a level ( k ). (n = 5–6; * p

    Article Snippet: Measurement of serum antibody Each mouse was bled at sacrifice, and individual sera were assayed for total IgE, IgG1 and IgG2a by ELISA on plates coated with goat anti-mouse Ab to IgG1, IgG2a (BD PharMingen) or rat anti-mouse Ab to IgE (BD PharMingen).

    Techniques: Infection, Real-time Polymerase Chain Reaction, Expressing, Mouse Assay, Cell Culture

    Lateral flow assay with aptamer-phage for IgE detection. Various concentrations of IgE (volume: 10 μL) were detected using anti-IgE antibodies at the test line and IgE aptamer-HRP-phage reporters. Control line consisted of anti-M13 antibodies.

    Journal: Analytical chemistry

    Article Title: Aptamer-phage reporters for ultrasensitive lateral flow assays

    doi: 10.1021/acs.analchem.5b00702

    Figure Lengend Snippet: Lateral flow assay with aptamer-phage for IgE detection. Various concentrations of IgE (volume: 10 μL) were detected using anti-IgE antibodies at the test line and IgE aptamer-HRP-phage reporters. Control line consisted of anti-M13 antibodies.

    Article Snippet: Human immunoglobulin E (IgE) was obtained from Abcam (Cambridge, MA, USA).

    Techniques: Lateral Flow Assay

    PBP2a aptamer-phage as a specificity control for IgE-aptamer-phage reporters. Strips 1 2; Strips with PBP2a protein and anti-M13 antibodies on the test and control lines, respectively, were made to evaluate the binding of PBP2a-M13 to PBP2a

    Journal: Analytical chemistry

    Article Title: Aptamer-phage reporters for ultrasensitive lateral flow assays

    doi: 10.1021/acs.analchem.5b00702

    Figure Lengend Snippet: PBP2a aptamer-phage as a specificity control for IgE-aptamer-phage reporters. Strips 1 2; Strips with PBP2a protein and anti-M13 antibodies on the test and control lines, respectively, were made to evaluate the binding of PBP2a-M13 to PBP2a

    Article Snippet: Human immunoglobulin E (IgE) was obtained from Abcam (Cambridge, MA, USA).

    Techniques: Binding Assay

    Functionalization of M13 phage with IgE aptamers and Horseradish peroxidase (HRP). Phage displaying AviTag peptides are biotinylated using Biotin ligase. Biotinylated phage are covalently modified with HRP on the major coat protein pVIII before neutravidin

    Journal: Analytical chemistry

    Article Title: Aptamer-phage reporters for ultrasensitive lateral flow assays

    doi: 10.1021/acs.analchem.5b00702

    Figure Lengend Snippet: Functionalization of M13 phage with IgE aptamers and Horseradish peroxidase (HRP). Phage displaying AviTag peptides are biotinylated using Biotin ligase. Biotinylated phage are covalently modified with HRP on the major coat protein pVIII before neutravidin

    Article Snippet: Human immunoglobulin E (IgE) was obtained from Abcam (Cambridge, MA, USA).

    Techniques: Modification

    Direct detection of spotted target analytes (PBP2a (A) or IgE (B)) with aptamer-phage. (A) PBP2a protein and anti-M13 antibodies were spotted on the test and control lines, respectively. (B) IgE protein and anti-M13 antibodies were spotted on the test

    Journal: Analytical chemistry

    Article Title: Aptamer-phage reporters for ultrasensitive lateral flow assays

    doi: 10.1021/acs.analchem.5b00702

    Figure Lengend Snippet: Direct detection of spotted target analytes (PBP2a (A) or IgE (B)) with aptamer-phage. (A) PBP2a protein and anti-M13 antibodies were spotted on the test and control lines, respectively. (B) IgE protein and anti-M13 antibodies were spotted on the test

    Article Snippet: Human immunoglobulin E (IgE) was obtained from Abcam (Cambridge, MA, USA).

    Techniques:

    Control for non-specific binding of IgE and aptamer-phage to an unrelated protein (the murine anti-lysozyme IgG antibody HyHEL-5). Strips 1 2; HyHEL-5 was spotted on the test line and anti-M13 antibodies on the control line. IgE protein (100

    Journal: Analytical chemistry

    Article Title: Aptamer-phage reporters for ultrasensitive lateral flow assays

    doi: 10.1021/acs.analchem.5b00702

    Figure Lengend Snippet: Control for non-specific binding of IgE and aptamer-phage to an unrelated protein (the murine anti-lysozyme IgG antibody HyHEL-5). Strips 1 2; HyHEL-5 was spotted on the test line and anti-M13 antibodies on the control line. IgE protein (100

    Article Snippet: Human immunoglobulin E (IgE) was obtained from Abcam (Cambridge, MA, USA).

    Techniques: Binding Assay

    Biotin-TEG IgE aptamer (secondary structure adapted from Reference 29).

    Journal: Analytical chemistry

    Article Title: Aptamer-phage reporters for ultrasensitive lateral flow assays

    doi: 10.1021/acs.analchem.5b00702

    Figure Lengend Snippet: Biotin-TEG IgE aptamer (secondary structure adapted from Reference 29).

    Article Snippet: Human immunoglobulin E (IgE) was obtained from Abcam (Cambridge, MA, USA).

    Techniques:

    Fc͛RI-bound IgE on effector cells. Light gray area: total bound IgE/cell (number of Fc͛RI occupied by IgE with population-based distribution) on basophilic leukocytes. Dark gray area: specific IgE/cell required for half-maximal cell activation (intrinsic sensitivity of basophils with population-based distribution). The distribution of both variables is approximately normal and can differ significantly; evidently, a fraction (ca. 1%) of bound total IgE is sufficient for half-maximal allergen specific activation. For this reason, the ratio of specific to total IgE is interesting in terms of interpretation. Inset top left: individual mediator release as a function of cell-bound specific IgE; basis for the population-based normal distributions illustrated in the lower part of the figure.

    Journal: Allergo Journal International

    Article Title: Molecular allergy diagnostics using IgE singleplex determinations: methodological and practical considerations for use in clinical routine

    doi: 10.1007/s40629-015-0067-z

    Figure Lengend Snippet: Fc͛RI-bound IgE on effector cells. Light gray area: total bound IgE/cell (number of Fc͛RI occupied by IgE with population-based distribution) on basophilic leukocytes. Dark gray area: specific IgE/cell required for half-maximal cell activation (intrinsic sensitivity of basophils with population-based distribution). The distribution of both variables is approximately normal and can differ significantly; evidently, a fraction (ca. 1%) of bound total IgE is sufficient for half-maximal allergen specific activation. For this reason, the ratio of specific to total IgE is interesting in terms of interpretation. Inset top left: individual mediator release as a function of cell-bound specific IgE; basis for the population-based normal distributions illustrated in the lower part of the figure.

    Article Snippet: Allergen molecules (synonyms: single allergens, allergen components) open up new horizons for the targeted allergen-specific diagnostics of immunoglobulin E (IgE) in singleplex determination.

    Techniques: Activation Assay

    Immunoglobulin E ( IgE ) levels to allergen molecules depending on structural similarity within an allergen family. a Variable, limited cross-reactivity between 2S albumins (stable storage proteins in nuts, pulses, and seeds). b Variable cross-reactivity between Bet v 1-homologous food allergens. c High cross-reactivity due to the strongly preserved and similar structure of profilins (in pollen, latex, and foods)

    Journal: Allergo Journal International

    Article Title: Molecular allergy diagnostics using IgE singleplex determinations: methodological and practical considerations for use in clinical routine

    doi: 10.1007/s40629-015-0067-z

    Figure Lengend Snippet: Immunoglobulin E ( IgE ) levels to allergen molecules depending on structural similarity within an allergen family. a Variable, limited cross-reactivity between 2S albumins (stable storage proteins in nuts, pulses, and seeds). b Variable cross-reactivity between Bet v 1-homologous food allergens. c High cross-reactivity due to the strongly preserved and similar structure of profilins (in pollen, latex, and foods)

    Article Snippet: Allergen molecules (synonyms: single allergens, allergen components) open up new horizons for the targeted allergen-specific diagnostics of immunoglobulin E (IgE) in singleplex determination.

    Techniques:

    Options for the evaluation of logarithmically distributed allergen-specific IgE levels. A quantitative; B semi-quantitative (since entry into force of the German Medical Association guideline, Richtlinie der Bundesärztekammer (RiliBÄK), this term is no longer provided for; specific IgE levels given only in classes are considered as qualitative evaluations); C qualitative. Allergen-specific IgE levels expressed as units of specific IgE, kUA/l (A stands for allergen-specific), using WHO standards for total IgE determination (heterologous calibration). Light gray area: population of serum samples with no allergen-specific IgE (levels fall below the detection limit of 0.1 kUA/l). Dark gray area: population of positive serum samples with logarithmic (hypothetically normal) distribution of allergen-specific IgE levels above the detection limit of 0.1 kUA/l

    Journal: Allergo Journal International

    Article Title: Molecular allergy diagnostics using IgE singleplex determinations: methodological and practical considerations for use in clinical routine

    doi: 10.1007/s40629-015-0067-z

    Figure Lengend Snippet: Options for the evaluation of logarithmically distributed allergen-specific IgE levels. A quantitative; B semi-quantitative (since entry into force of the German Medical Association guideline, Richtlinie der Bundesärztekammer (RiliBÄK), this term is no longer provided for; specific IgE levels given only in classes are considered as qualitative evaluations); C qualitative. Allergen-specific IgE levels expressed as units of specific IgE, kUA/l (A stands for allergen-specific), using WHO standards for total IgE determination (heterologous calibration). Light gray area: population of serum samples with no allergen-specific IgE (levels fall below the detection limit of 0.1 kUA/l). Dark gray area: population of positive serum samples with logarithmic (hypothetically normal) distribution of allergen-specific IgE levels above the detection limit of 0.1 kUA/l

    Article Snippet: Allergen molecules (synonyms: single allergens, allergen components) open up new horizons for the targeted allergen-specific diagnostics of immunoglobulin E (IgE) in singleplex determination.

    Techniques:

    Significance of the total and specific immunoglobulin E (IgE) ratio. Due to the variability of total IgE levels, logarithmically distributed specific IgE (dark gray bars) can also be expressed as a relative quantity of total IgE (light gray bars) [ 16 ]. This process „normalizes“ specific IgE to total IgE on a percentage basis (hatched bars). Primarily the borderline cases (see numerical examples) with particularly low (normal distribution curve, far left) or extremely high total IgE (normal distribution curve, far right) make it clear that specific IgE can only be correctly interpreted once total IgE is known. This ratio of specific to total IgE is also found on the surface of effector cells (mast cells, basophil granulocytes), thereby providing the basis for diagnostic ex vivo (basophil activation test, BAT) and in vivo tests (skin prick test, provocation test)

    Journal: Allergo Journal International

    Article Title: Molecular allergy diagnostics using IgE singleplex determinations: methodological and practical considerations for use in clinical routine

    doi: 10.1007/s40629-015-0067-z

    Figure Lengend Snippet: Significance of the total and specific immunoglobulin E (IgE) ratio. Due to the variability of total IgE levels, logarithmically distributed specific IgE (dark gray bars) can also be expressed as a relative quantity of total IgE (light gray bars) [ 16 ]. This process „normalizes“ specific IgE to total IgE on a percentage basis (hatched bars). Primarily the borderline cases (see numerical examples) with particularly low (normal distribution curve, far left) or extremely high total IgE (normal distribution curve, far right) make it clear that specific IgE can only be correctly interpreted once total IgE is known. This ratio of specific to total IgE is also found on the surface of effector cells (mast cells, basophil granulocytes), thereby providing the basis for diagnostic ex vivo (basophil activation test, BAT) and in vivo tests (skin prick test, provocation test)

    Article Snippet: Allergen molecules (synonyms: single allergens, allergen components) open up new horizons for the targeted allergen-specific diagnostics of immunoglobulin E (IgE) in singleplex determination.

    Techniques: Diagnostic Assay, Ex Vivo, Activation Assay, In Vivo

    While binding-induced changes in the flexibility of folded aptamers sometimes support E-AB signaling, the magnitude of this effect varies dramatically from aptamer-to-aptamer. (Left) For example, even though IgE is a rather larger target than thrombin, the binding of IgE (at a saturating concentration of 200 nM) to its (folded) aptamer produces only a small change in E-AB current. The magnitude of this change is similar to that observed for buffer blanks, which vary slightly due to minor sensor degradation (at the ca. 1% level over this timescale). (Right) Quartz crystal microbalance experiments confirm that the immobilized aptamer retains its ability to bind target and thus demonstrate that poor signaling, rather than poor binding, underlies the limited E-AB signaling.

    Journal: Electroanalysis

    Article Title: On the Signaling of Electrochemical Aptamer-Based Sensors: Collision- and Folding-Based Mechanisms

    doi: 10.1002/elan.200804564

    Figure Lengend Snippet: While binding-induced changes in the flexibility of folded aptamers sometimes support E-AB signaling, the magnitude of this effect varies dramatically from aptamer-to-aptamer. (Left) For example, even though IgE is a rather larger target than thrombin, the binding of IgE (at a saturating concentration of 200 nM) to its (folded) aptamer produces only a small change in E-AB current. The magnitude of this change is similar to that observed for buffer blanks, which vary slightly due to minor sensor degradation (at the ca. 1% level over this timescale). (Right) Quartz crystal microbalance experiments confirm that the immobilized aptamer retains its ability to bind target and thus demonstrate that poor signaling, rather than poor binding, underlies the limited E-AB signaling.

    Article Snippet: Thrombin obtained from Haematologic Technologies Inc. (Essex Junction, VT) and Immunoglobulin E (IgE, human plasma) purchased from Athens research & Technology (Georgia, USA) were used as received.

    Techniques: Binding Assay, Concentration Assay

    No cross-reactivity between bGST and HDM-GST. (A) IgG1-reactivity of sera (n = 3) collected from bGST-immunized mice at day 0 and day 59 to BPE and HDME. (B) IgE-reactivity of seven Der p 8-sensitized HDM-allergic patients to bGST and HDME. NHS, non-allergic control sera; O.D. optical density; dotted and dashed lines indicate the cut-off for positive IgE-reactivity.

    Journal: PLoS ONE

    Article Title: Glutathione-S-Transferase: A Minor Allergen in Birch Pollen due to Limited Release from Hydrated Pollen

    doi: 10.1371/journal.pone.0109075

    Figure Lengend Snippet: No cross-reactivity between bGST and HDM-GST. (A) IgG1-reactivity of sera (n = 3) collected from bGST-immunized mice at day 0 and day 59 to BPE and HDME. (B) IgE-reactivity of seven Der p 8-sensitized HDM-allergic patients to bGST and HDME. NHS, non-allergic control sera; O.D. optical density; dotted and dashed lines indicate the cut-off for positive IgE-reactivity.

    Article Snippet: Briefly, all patients suffered from rhinoconjunctivitis in spring and showed positive skin prick test responses to BPE and specific IgE to BP (≥0.35 kUA /L, ImmunoCAP, Thermofisher, Uppsala, Sweden).

    Techniques: Mouse Assay

    IgE-responses of BP-allergic individuals. (A) IgE-reactivity of 217 BP-allergic patients to BP allergens and bGST. (B) IgE-reactivity to bGST is inhibited by pre-incubation of sera with BPE (gray line) and bGST (black line). One representative example of four is shown. (C) RBL assay with sera from three patients sensitized to bGST and Bet v 1. Degranulation was induced with titrated amounts of bGST (filled circles) and Bet v 1 (open circles). The release of β-hexosaminidase is expressed as percentage of the release from anti-IgE-treated cells.

    Journal: PLoS ONE

    Article Title: Glutathione-S-Transferase: A Minor Allergen in Birch Pollen due to Limited Release from Hydrated Pollen

    doi: 10.1371/journal.pone.0109075

    Figure Lengend Snippet: IgE-responses of BP-allergic individuals. (A) IgE-reactivity of 217 BP-allergic patients to BP allergens and bGST. (B) IgE-reactivity to bGST is inhibited by pre-incubation of sera with BPE (gray line) and bGST (black line). One representative example of four is shown. (C) RBL assay with sera from three patients sensitized to bGST and Bet v 1. Degranulation was induced with titrated amounts of bGST (filled circles) and Bet v 1 (open circles). The release of β-hexosaminidase is expressed as percentage of the release from anti-IgE-treated cells.

    Article Snippet: Briefly, all patients suffered from rhinoconjunctivitis in spring and showed positive skin prick test responses to BPE and specific IgE to BP (≥0.35 kUA /L, ImmunoCAP, Thermofisher, Uppsala, Sweden).

    Techniques: Incubation

    (a) Concentrations of total immunoglobulin E (IgE; ng/ml) and (b) levels of insulin-specific IgG1 in the plasma of mice that were implanted intraperitoneally with five female or male adult worms ( n = 12) or sham-treated controls ( n = 11). Significant differences

    Journal: Immunology

    Article Title: Inhibition of type 1 diabetes in filaria-infected non-obese diabetic mice is associated with a T helper type 2 shift and induction of FoxP3+ regulatory T cells

    doi: 10.1111/j.1365-2567.2008.02958.x

    Figure Lengend Snippet: (a) Concentrations of total immunoglobulin E (IgE; ng/ml) and (b) levels of insulin-specific IgG1 in the plasma of mice that were implanted intraperitoneally with five female or male adult worms ( n = 12) or sham-treated controls ( n = 11). Significant differences

    Article Snippet: Cells were plated in triplicate at a concentration of 2 × 106 cells/ml in a volume of 100 μl immunoglobulin E (IgE) medium (Iscove’s modified Dulbecco’s medium; Mediatech) including 10% fetal calf serum (Valley Biomedical, Winchester, VA), 1% l -glutamine (Mediatech), 1% insulin-transferrin-selenium medium (Invitrogen Inc.) and 80 μg/ml gentamicin (Invitrogen Inc.).

    Techniques: Mouse Assay

    Images and linescan plots recorded at various time points during an μFFE gradient where a constant concentration of fluorescently labeled aptamer for IgE was titrated with an increasing concentration of IgE. Peaks from left to right were identified as the unbound aptamer, the aptamer-IgE complex, and the internal standard (rhodamine 110). The anode is at the left side of the images.

    Journal: Analytical chemistry

    Article Title: Measuring Aptamer Equilbria Using Gradient Micro Free Flow Electrophoresis

    doi: 10.1021/ac902877v

    Figure Lengend Snippet: Images and linescan plots recorded at various time points during an μFFE gradient where a constant concentration of fluorescently labeled aptamer for IgE was titrated with an increasing concentration of IgE. Peaks from left to right were identified as the unbound aptamer, the aptamer-IgE complex, and the internal standard (rhodamine 110). The anode is at the left side of the images.

    Article Snippet: Human myeloma immunoglobulin E (IgE) (1.1 mg/mL) in solution was obtained from Athens Research and Technology (Athens, GA, USA).

    Techniques: Concentration Assay, Labeling

    A) A contour plot showing subsequent μFFE linescans measured over time during the IgE concentration gradient. Peaks are identified from left (anode) to right as 1) the free aptamer, 2) the aptamer-IgE complex, and 3) the internal standard (rhodamine 110). B) A plot of peak area over time for the free aptamer, aptamer-IgE complex and internal standard. Black bars on both plots represent the 5 minute interval over which the concentration of IgE was increased from 0 to 500 nM.

    Journal: Analytical chemistry

    Article Title: Measuring Aptamer Equilbria Using Gradient Micro Free Flow Electrophoresis

    doi: 10.1021/ac902877v

    Figure Lengend Snippet: A) A contour plot showing subsequent μFFE linescans measured over time during the IgE concentration gradient. Peaks are identified from left (anode) to right as 1) the free aptamer, 2) the aptamer-IgE complex, and 3) the internal standard (rhodamine 110). B) A plot of peak area over time for the free aptamer, aptamer-IgE complex and internal standard. Black bars on both plots represent the 5 minute interval over which the concentration of IgE was increased from 0 to 500 nM.

    Article Snippet: Human myeloma immunoglobulin E (IgE) (1.1 mg/mL) in solution was obtained from Athens Research and Technology (Athens, GA, USA).

    Techniques: Concentration Assay

    Binding curves for the aptamer-IgE equilibrium measured using A) gradient μFFE and B) fluorescence. Dissociation constants (K d ) of the aptamer-IgE complex estimated using the μFFE and fluorescence assays were 48±3 nM and 24±4 nM, respectively.

    Journal: Analytical chemistry

    Article Title: Measuring Aptamer Equilbria Using Gradient Micro Free Flow Electrophoresis

    doi: 10.1021/ac902877v

    Figure Lengend Snippet: Binding curves for the aptamer-IgE equilibrium measured using A) gradient μFFE and B) fluorescence. Dissociation constants (K d ) of the aptamer-IgE complex estimated using the μFFE and fluorescence assays were 48±3 nM and 24±4 nM, respectively.

    Article Snippet: Human myeloma immunoglobulin E (IgE) (1.1 mg/mL) in solution was obtained from Athens Research and Technology (Athens, GA, USA).

    Techniques: Binding Assay, Fluorescence

    Peptide ELISA demonstrating the binding of Met e 1-specific IgE to the mimotopes. Mimotope KGIPNTKAP of Der p 1 and an IgE-binding epitope of Met e 1 (a.a. 251-270) were included as negative and positive controls, respectively. Met e 1-specific IgE could exclusively recognize the selected mimotopes from clusters 1–6, but not the irrelevant mimotope of Der p 1 ( P

    Journal: Cellular and Molecular Immunology

    Article Title: Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one-compound peptide libraries

    doi: 10.1038/cmi.2015.83

    Figure Lengend Snippet: Peptide ELISA demonstrating the binding of Met e 1-specific IgE to the mimotopes. Mimotope KGIPNTKAP of Der p 1 and an IgE-binding epitope of Met e 1 (a.a. 251-270) were included as negative and positive controls, respectively. Met e 1-specific IgE could exclusively recognize the selected mimotopes from clusters 1–6, but not the irrelevant mimotope of Der p 1 ( P

    Article Snippet: Increasing number of studies have demonstrated the success of mimotope-based therapy in various diseases., , , , Inspired by the unique property of mimotopes to induce epitope-specific antibodies, we investigated the capacity of mimotopes to inhibit immunoglobulin E (IgE)-allergen binding., , Due to their monovalent properties, they are safer than natural extracts/recombinant allergens in allergen-specific immunotherapy (SIT), and can prevent anaphylaxis caused by cross-linking of IgE and degranulation of mast cells.

    Techniques: Peptide ELISA, Binding Assay

    Levels of IgE and IgG4 to Schistosoma mansoni tropomyosin (Tpm)II isoforms. Levels of IgE (A) and IgG4 (B) to TpmII isoforms were measured in a population of infected boys and men ( n = 228) from an area endemic for S. mansoni , both before and 7 weeks after treatment with Praziquantel. Shown are the levels (geometric mean ± 95% confidence interval) for those with a detectable response. The prevalence (%) of each response (before treatment) in the cohort is indicated.

    Journal: International Journal for Parasitology

    Article Title: Human IgE responses to different splice variants of Schistosoma mansoni tropomyosin: associations with immunity

    doi: 10.1016/j.ijpara.2014.02.004

    Figure Lengend Snippet: Levels of IgE and IgG4 to Schistosoma mansoni tropomyosin (Tpm)II isoforms. Levels of IgE (A) and IgG4 (B) to TpmII isoforms were measured in a population of infected boys and men ( n = 228) from an area endemic for S. mansoni , both before and 7 weeks after treatment with Praziquantel. Shown are the levels (geometric mean ± 95% confidence interval) for those with a detectable response. The prevalence (%) of each response (before treatment) in the cohort is indicated.

    Article Snippet: Standard curves were generated for every plate with the O.D. values obtained from the wells coated with serial dilutions of human myeloma IgE (Calbiochem, USA) or IgG4 (Sigma).

    Techniques: Infection

    Prevalence of anti- Schistosoma mansoni tropomyosin (Tpm)II IgE and IgG4 responses by age of participant. The pre-treatment prevalence data for the IgE and IgG4 responses to (A) TpmII.3, (B) TpmII.4, (C) TpmII.7 and (D) TpmII.8 are shown for five age groups: 7–9 years ( n = 39), 10–14 years ( n = 46), 15–25 years ( n = 45), 26–36 years ( n = 50) and > 37 years ( n = 48). The% of each group with a detectable response is plotted against the mean age of the group. Error bars represent 95% confidence intervals.

    Journal: International Journal for Parasitology

    Article Title: Human IgE responses to different splice variants of Schistosoma mansoni tropomyosin: associations with immunity

    doi: 10.1016/j.ijpara.2014.02.004

    Figure Lengend Snippet: Prevalence of anti- Schistosoma mansoni tropomyosin (Tpm)II IgE and IgG4 responses by age of participant. The pre-treatment prevalence data for the IgE and IgG4 responses to (A) TpmII.3, (B) TpmII.4, (C) TpmII.7 and (D) TpmII.8 are shown for five age groups: 7–9 years ( n = 39), 10–14 years ( n = 46), 15–25 years ( n = 45), 26–36 years ( n = 50) and > 37 years ( n = 48). The% of each group with a detectable response is plotted against the mean age of the group. Error bars represent 95% confidence intervals.

    Article Snippet: Standard curves were generated for every plate with the O.D. values obtained from the wells coated with serial dilutions of human myeloma IgE (Calbiochem, USA) or IgG4 (Sigma).

    Techniques:

    Binding of Sjc23-LED with immunoglobulins in pull-down assay. A Human IgG (lane 1), IgA (lane 4), IgE (lane 6), and IgM (lane 8) were incubated with Sjc23-LED bound Sepharose resin, after extensive washing, the proteins were resolved in SDS-PAGE and blotted to nylon film. The immunoglobulins that bound to Sjc23-LED was detected by using mAbs specific for human antibodies (γ-chain, α-chain,ε-chain or µ-chain specific). Only human IgG could specifically bind to Sjc23-LED, whereas the other antibody types did not. GST did not bind human IgG (lane 2). Lanes 3, 5, 7 and 9 were corresponding antibodies as controls for detection. B Porcine and bovine IgG was respectively incubated with Sjc23-LED bound Sepharose resin and only porcine IgG was marginally precipitated (lane 1, the band was indicated with an asterisk). Lane 2 and 4 were controls of the corresponding IgGs.

    Journal: PLoS ONE

    Article Title: Mapping the Binding between the Tetraspanin Molecule (Sjc23) ofSchistosoma japonicum and Human Non-Immune IgG

    doi: 10.1371/journal.pone.0019112

    Figure Lengend Snippet: Binding of Sjc23-LED with immunoglobulins in pull-down assay. A Human IgG (lane 1), IgA (lane 4), IgE (lane 6), and IgM (lane 8) were incubated with Sjc23-LED bound Sepharose resin, after extensive washing, the proteins were resolved in SDS-PAGE and blotted to nylon film. The immunoglobulins that bound to Sjc23-LED was detected by using mAbs specific for human antibodies (γ-chain, α-chain,ε-chain or µ-chain specific). Only human IgG could specifically bind to Sjc23-LED, whereas the other antibody types did not. GST did not bind human IgG (lane 2). Lanes 3, 5, 7 and 9 were corresponding antibodies as controls for detection. B Porcine and bovine IgG was respectively incubated with Sjc23-LED bound Sepharose resin and only porcine IgG was marginally precipitated (lane 1, the band was indicated with an asterisk). Lane 2 and 4 were controls of the corresponding IgGs.

    Article Snippet: The three proteins, Sjc23-LED, GST and TSP-2, were incubated respectively with purified human IgG, IgM, IgA (Sigma, CA, USA) and IgE (Abcam, Cambridge, UK).

    Techniques: Binding Assay, Pull Down Assay, Incubation, SDS Page

    BV reduces OVA-induced mast cells, serum IgE and pro-inflammatory cytokine expression. (A) Giemsa staining: Representative images of histological analysis, presenting increased infiltration and degranulation of the mast cells. However, it was reduced under BV treatment. Magnification, ×400; scale bars, 50 µm. (B) Infiltration and degranulation were evaluated by assessing the number of mast cells from at least 3 random fields per section at 400-fold magnification. (C) The ELISA results demonstrated that BV inhibited the increased levels of serum-IgE induced by OVA. In addition, BV inhibited the increased expression of inflammatory cytokines such as (D) TNF-α and (E) TSLP induced by OVA. The results are expressed as the mean ± standard error of the mean of 3 independent determinations. *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of bee venom on experimental atopic dermatitis

    doi: 10.3892/mmr.2018.9398

    Figure Lengend Snippet: BV reduces OVA-induced mast cells, serum IgE and pro-inflammatory cytokine expression. (A) Giemsa staining: Representative images of histological analysis, presenting increased infiltration and degranulation of the mast cells. However, it was reduced under BV treatment. Magnification, ×400; scale bars, 50 µm. (B) Infiltration and degranulation were evaluated by assessing the number of mast cells from at least 3 random fields per section at 400-fold magnification. (C) The ELISA results demonstrated that BV inhibited the increased levels of serum-IgE induced by OVA. In addition, BV inhibited the increased expression of inflammatory cytokines such as (D) TNF-α and (E) TSLP induced by OVA. The results are expressed as the mean ± standard error of the mean of 3 independent determinations. *P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The concentrations of IgE, tumor necrosis factor (TNF)-α, and TSLP in the collected sera of the blood were determined by ELISA kit, according to the manufacturer's instructions (IgE; Bethyl Laboratories, Montgomery, TX, USA) and R & D Systems (TNF-α and TSLP).

    Techniques: Expressing, Staining, Enzyme-linked Immunosorbent Assay