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Image Search Results
Journal: Parasitology
Article Title: IgM + memory B cells induced in response to Plasmodium berghei adopt a germinal centre B cell phenotype during secondary infection
doi: 10.1017/S003118202000061X
Figure Lengend Snippet: Plasmodium berghei ANKA infection induces IgM+ memory B cells. C57BL/6 mice were infected with P. berghei ANKA and treated with anti-malarial drugs from day 5 pi. (A) Gating strategy used to identify IgM+ memory B cells by flow cytometry, depicting gated IgD−CD19+ B cells that were negative for CD21 and CD138 to exclude marginal zone B cells and plasma cells, respectively. Memory B cells were then identified by high expression of CD38 and low levels of Fas, to exclude GC B cells. IgM+ memory B cells were identified among this compartment. (B) Absolute number of IgM+ memory B cells in P. berghei ANKA-infected mice on day 24 pi and naive controls. Error bars represent the mean of 8–10 mice ± s.e.m., ***P < 0.005.
Article Snippet: To detect of IgM + memory B cells, splenocytes were stained with: APC anti-CD21 (7G6, Miltenyi Biotec), FITC anti-IgM (Il/41, BD Biosciences),
Techniques: Infection, Flow Cytometry, Expressing