Journal:

Article Title: Lipopolysaccharide- and Cholera Toxin-Specific Subclass Distribution of B-Cell Responses in Cholera

doi:

Figure Lengend Snippet: CT-specific ASC response of the IgG and IgA subclasses in V. cholerae O1 and V. cholerae O139-infected patients. ASC responses (GM ± SEM) recorded on day 7 after onset of illness are shown. PR, number of patients showing a positive ASC response to CT/total number studied.

Article Snippet: For the IgA subclass responses, biotin-labeled mouse anti-human IgA1 (clone B3506B4) and IgA2 (clone A9604D2) antibodies (Southern Biotechnology Associates [SBA], Birmingham, Ala.) were used.

Techniques: Infection

Journal:

Article Title: Lipopolysaccharide- and Cholera Toxin-Specific Subclass Distribution of B-Cell Responses in Cholera

doi:

Figure Lengend Snippet: CT- and LPS-specific antibody responses, in plasma, of the IgA1 (A) and the IgA2 (B) subclasses in V. cholerae O1- and V. cholerae O139-infected patients. The GMT and SEM are shown. Asterisks indicate statistically significant differences in the responses between the acute stage (day [d] 2) and at convalescence (day 11): ∗, P < 0.05 to 0.01; ∗∗, P < 0.01 to 0.001; ∗∗∗, P < 0.001. PR, number of patients showing positive response/total number of patients studied.

Article Snippet: For the IgA subclass responses, biotin-labeled mouse anti-human IgA1 (clone B3506B4) and IgA2 (clone A9604D2) antibodies (Southern Biotechnology Associates [SBA], Birmingham, Ala.) were used.

Techniques: Infection

Journal:

Article Title: Lipopolysaccharide- and Cholera Toxin-Specific Subclass Distribution of B-Cell Responses in Cholera

doi:

Figure Lengend Snippet: IgA subclass responses to rCTB and homologous LPS in fecal extracts from cholera patients

Article Snippet: For the IgA subclass responses, biotin-labeled mouse anti-human IgA1 (clone B3506B4) and IgA2 (clone A9604D2) antibodies (Southern Biotechnology Associates [SBA], Birmingham, Ala.) were used.

Techniques:

Journal: Clinical Journal of the American Society of Nephrology

Article Title: StaphylococcusInfection-Associated Glomerulonephritis Mimicking IgA Nephropathy

doi: 10.2215/cjn.01030306

Figure Lengend Snippet: Figure 3. Direct immunofluorescence showing granular mesan- gial staining for IgA in the expanded mesangium of the biopsy of patient 4, who had underlying diabetic glomerulosclerosis. The IgA fluorescence was not strong in most cases. Magnifica- tion 400.

Article Snippet: We also used monoclonal antibodies to IgA1 and IgA2 (Novus Biologicals, Littleton, CO) with an indirect IF methodology.

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: Clinical Journal of the American Society of Nephrology

Article Title: StaphylococcusInfection-Associated Glomerulonephritis Mimicking IgA Nephropathy

doi: 10.2215/cjn.01030306

Figure Lengend Snippet: Figure 4. Strong fluorescence for IgA1 in the biopsy of patient 5, who had large, cryoglobulin-like subendothelial deposits. Mag- nification, 400.

Article Snippet: We also used monoclonal antibodies to IgA1 and IgA2 (Novus Biologicals, Littleton, CO) with an indirect IF methodology.

Techniques: Fluorescence

Journal: eLife

Article Title: IFNγ induces epigenetic programming of human T-bet hi B cells and promotes TLR7/8 and IL-21 induced differentiation

doi: 10.7554/eLife.41641

Figure Lengend Snippet: Gating strategy to identify unswitched and isotype switched ASCs in Be1 and Be2 co-cultures. Representative flow plots showing intracellular IgM, IgA and IgG staining ( b ) on ASC-gated cells (from panel a ) in paired Be1 and Be2 co-cultures. Data are reported as the frequency of intracellular IgM, IgG or IgA expressing CD19 lo CD38 hi ASCs within either the total CD19 +/lo B lineage compartment (black font) or within the total ASCs (bold blue font).

Article Snippet: Antibody , PE Mouse Anti-Human IgA(2 ) (clone IS11-21E11 ) , Miltenyi , 130-100-316 , (1:200).

Techniques: Staining, Expressing

Journal: eLife

Article Title: IFNγ induces epigenetic programming of human T-bet hi B cells and promotes TLR7/8 and IL-21 induced differentiation

doi: 10.7554/eLife.41641

Figure Lengend Snippet:

Article Snippet: Antibody , PE Mouse Anti-Human IgA(2 ) (clone IS11-21E11 ) , Miltenyi , 130-100-316 , (1:200).

Techniques: Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Staining, Cell Isolation, Sterility, Membrane, Recombinant, Enzyme-linked Immunospot, Purification