Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Alterations in Gut Microbiome-Host Relationships After Immune Perturbation in Patients With Multiple Sclerosis

doi: 10.1212/NXI.0000000000200355

Figure Lengend Snippet: Flow cytometry was used to determine the proportions of (A) IgA-coated and (B) IgM-coated bacteria. (C) Total secreted, unbound IgA was quantified using ELISA. Multiple linear regression models, adjusted for age, sex, BMI, and steroid use, were applied in (A–C) to assess the significance of differences between controls and patients with untreated MS. BMI = body mass index; IgA = immunoglobulin A; MS = multiple sclerosis.

Article Snippet: Soluble, unbound IgA in the fecal supernatants was quantified using an ELISA kit (Eagle Biosciences, SGA35-K01).

Techniques: Flow Cytometry, Bacteria, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Altered B cell immunoglobulin signature exhibits potential diagnostic values in human colorectal cancer

doi: 10.1016/j.isci.2023.106140

Figure Lengend Snippet: Biased IgG subclass distribution in CRC (A) FACS analysis was performed to determine the phenotypic characteristics of B cells in normal mucosa and B cells in tumor tissue. Representative markers expressed (N = 7). (B) Statistical analysis of the phenotypic characteristics and statistical significance were determined using the Mann-Whitney U test, N = 5, results represent mean ± SEM of five donors (N = 7), ∗p < 0.05, ∗∗p < 0.01. (C and D) Confocal microscopy analysis and statistical analysis of IgA+ and IgG+ cells (red) in CD79a+ B cells (green). (E) The conditioned medium was collected from isolated CD45 + CD19 + B cells after 24 h, and IgM, IgA, and IgG level in the conditioned medium was determined by ELISA. Results represent mean ± SEM of four independent experiments (N = 5). ∗∗p < 0.01; ∗∗∗p < 0.001 (Paired Student’s t test).

Article Snippet: Human IgA ELISA Kit , Bethyl Laboratories , Cat#E88-102.

Techniques: MANN-WHITNEY, Confocal Microscopy, Isolation, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Altered B cell immunoglobulin signature exhibits potential diagnostic values in human colorectal cancer

doi: 10.1016/j.isci.2023.106140

Figure Lengend Snippet:

Article Snippet: Human IgA ELISA Kit , Bethyl Laboratories , Cat#E88-102.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: Frontiers in immunology

Article Title: Interleukin (IL)-21 Promotes the Differentiation of IgA-Producing Plasma Cells in Porcine Peyer's Patches via the JAK-STAT Signaling Pathway.

doi: 10.3389/fimmu.2020.01303

Figure Lengend Snippet: FIGURE 1 | IL-21 increases IgA secretion in IgM+ B cells from porcine PPs and MLNs. IgM+ B lymphocytes from porcine Peyer’s patches and MLNs were isolated and cultured with the indicated factors and cytokines for 5–6 days. The IgA and IgM levels secreted by porcine IgM+ B cells from Peyer’s patches (A,B) and MLNs (C,D) were measured by ELISA. Experiments were performed independently in duplicate and triplicate. *p ≤0.05; **p ≤0.01; ***p ≤0.001; ****p ≤0.0001.

Article Snippet: After a 5-day culture of B cells with different factors and cytokines, as described before, the expression levels of IgM and IgA in culture medium were measured using a Pig IgM/IgA ELISA Kit (Bethyl Laboratories) according to the manufacturer’s instructions.

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in immunology

Article Title: Interleukin (IL)-21 Promotes the Differentiation of IgA-Producing Plasma Cells in Porcine Peyer's Patches via the JAK-STAT Signaling Pathway.

doi: 10.3389/fimmu.2020.01303

Figure Lengend Snippet: FIGURE 6 | The JAK-STAT signaling pathway is involved in IL-21-induced IgA CSR. IgM+ B cells from porcine Peyer’s patches were cultured with different concentrations of solcitinib (A) or fludarabine (B) for 24 h and then stimulated with CD40L+IL21 for 2 h. The total and phosphorylated amounts of JAK1, STAT1, and STAT3 proteins were assessed by western blotting, and their density ratio was also analyzed and shown. IgM+ B cells were treated with 2 µM solcitinib or 50 µM fludarabine for 24 h and were then cultured with CD40L+IL-21 for 5 days. The cells were then collected at 60 h post-treatment and subjected to semi-quantitative PCR to assess the expression levels of GLTα, PSTα, and CTα (C), while cell supernatants were collected, and IgA levels were detected by ELISA (D). All experiments were performed independently in duplicate or triplicate. *p ≤0.05; **p ≤0.01; ***p ≤0.001.

Article Snippet: After a 5-day culture of B cells with different factors and cytokines, as described before, the expression levels of IgM and IgA in culture medium were measured using a Pig IgM/IgA ELISA Kit (Bethyl Laboratories) according to the manufacturer’s instructions.

Techniques: Cell Culture, Western Blot, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay