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Image Search Results
Journal: bioRxiv
Article Title: A Murine Model for Enhancement of Streptococcus pneumoniae Pathogenicity Upon Viral Infection and Advanced Age
doi: 10.1101/2020.07.29.227991
Figure Lengend Snippet: Young and aged C57BL/6 male mice were co-infected with S. pneumoniae and Influenza A virus PR8. 18 and 48 hours following IAV-infection (see experimental design in ), the lungs were harvested. (A) Lungs were stained with Hematoxylin and Eosin and shown are representative photographs at 100x or 400X (in inset). (B) Cytokines in the supernatants of lung homogenates of young (n=4) or aged (n=5) mice at 18 hours post co-infection were measured by multiplex ELISA. Asterisks represent statistical significance by Student’s t-test. (C) The percentages and total number of PMNs (Ly6G+) in the lungs was measured by flow cytometry. Young mice are represented by open bars and aged mice by shaded bars. The mean +/-SEM pooled from three separate experiments are shown where data are pooled from twelve mice per age group, except for 48h post-co-infection, where due to the kinetics of disease, data from only 7 surviving old mice are shown. Statistically significant differences determined by Student’s t-test are indicated by asterisks.
Article Snippet: IFNα was measured using
Techniques: Infection, Virus, Staining, Multiplex Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry
Journal: bioRxiv
Article Title: A Murine Model for Enhancement of Streptococcus pneumoniae Pathogenicity Upon Viral Infection and Advanced Age
doi: 10.1101/2020.07.29.227991
Figure Lengend Snippet: Young and aged C57BL/6 male mice were either co-infected with S. pneumoniae and Influenza A virus PR8 ( Sp + IAV), challenged with virus alone (IAV) or mock challenged with PBS (uninfected) (as in ). Two days post IAV-infection, (A) viral burdens in the lungs were determined and (B) levels of IFN-α in the supernatants of lung homogenates were measured by ELISA. Data from n=4 mice per group are shown. Statistically significant differences were determined by Student’s t-test.
Article Snippet: IFNα was measured using
Techniques: Infection, Virus, Enzyme-linked Immunosorbent Assay