Journal: Frontiers in Aging Neuroscience

Article Title: Royal Jelly Alleviates Cognitive Deficits and β-Amyloid Accumulation in APP/PS1 Mouse Model Via Activation of the cAMP/PKA/CREB/BDNF Pathway and Inhibition of Neuronal Apoptosis

doi: 10.3389/fnagi.2018.00428

Figure Lengend Snippet: RJ treatment reduced the brain Aβ burden of APP/PS1 mice. (A,B) The levels of SDS-soluble and -insoluble (formic acid soluble) Aβ were measured using enzyme-linked immunosorbent assays (ELISAs). (C) The Aβ plaques in the cortex and hippocampus were estimated after immunohistochemical (IHC) staining with the Aβ antibody (B-4), the number of plaques per view ( D,E ; cortex, 20× magnification; hippocampus, 40× magnification) and proportions of the positive area (F,G) were calculated, three sections per animal. Scale bar = 200 μm. The arrows point out the representative senile plaques (SPs). Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, n.s. non-significant. (H–L) Immunoblot analysis of β-secretase (BACE1), insulin-degrading enzyme (IDE), neprilysin (NEP) and lipoprotein receptor-related protein-1 (LRP-1) in the brain homogenates. n = 6–10 mice per group. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, n.s. non-significant.

Article Snippet: Briefly, sections were blocked with 3% BSA for 30 min, then incubated with primary antibodies overnight at 4°C, including BACE1 (1:100, Santa Cruz Biotechnology, USA), IDE (1:100, Santa Cruz Biotechnology, USA), β-amyloid (B-4, 1:100, Santa Cruz Biotechnology, USA).

Techniques: Immunohistochemical staining, Immunohistochemistry, Western Blot

Journal: Stem Cell Research & Therapy

Article Title: Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol

doi: 10.1186/s13287-015-0181-3

Figure Lengend Snippet: Hepatic lineage differentiation condition

Article Snippet: First, the cells were treated for 1 day of endodermal induction with RPMI-1640 (Gibco) supplemented with 100 nM IDE1 (Tocris, Chengdu, Sichuan, P.R.

Techniques:

Journal:

Article Title: Inhibitory effect of losartan, an AT1 angiotensin II receptor antagonist, on increased leucocyte entrapment in retinal microcirculation of diabetic rats

doi:

Figure Lengend Snippet: Leucocytes trapped in the retinal microcirculation were observed as fluorescent dots 30 minutes after acridine orange injection in an untreated diabetic rat (A), a control rat (B), and a diabetic rat treated with losartan (C).

Article Snippet: The first subgroup (n = 6) was left untreated; the second subgroup (n = 6) was given fresh drinking water supplemented with losartan potassium (Mikromol GmbH, Luckenwalde, Germany; concentration 50 mg/l).

Techniques: Injection

Journal:

Article Title: Inhibitory effect of losartan, an AT1 angiotensin II receptor antagonist, on increased leucocyte entrapment in retinal microcirculation of diabetic rats

doi:

Figure Lengend Snippet: Leucocytes trapped in the retinal microcirculation. Values are means (SD). *p<0.05 compared with control rats and diabetic rats treated with losartan.

Article Snippet: The first subgroup (n = 6) was left untreated; the second subgroup (n = 6) was given fresh drinking water supplemented with losartan potassium (Mikromol GmbH, Luckenwalde, Germany; concentration 50 mg/l).

Techniques: