Journal: Nature immunology

Article Title: Homeodomain only protein is required for the function of induced regulatory T cells in dendritic cell-mediated peripheral T cell unresponsiveness

doi: 10.1038/ni.1929

Figure Lengend Snippet: Hopx-dependent expression of T cell lineage-specific genes in DC-induced T reg cells Hopx −/− and Hopx +/+ OTII FOXP3-IRES-RFP littermate mice were treated with 50 ng of αDEC-OVA and after 7 days re-challenged by immunization with 100 µg OVA peptide in CFA. TCRα2 + CD4 + CD25 + RFP + (Foxp3 + ) DC-induced T reg cells were isolated 24h after immunization with OVA in CFA by cell sorting and total gene expression was measured using Affymetrix Mouse Genome 430 2.0 Arrays as in Fig. 3 . Results show fold-change of a particular gene expression in Hopx -deficient DC-induced T reg cells compared to its expression level in Hopx -sufficient DC-induced T reg cells of multiple major Foxp3-dependent and T cell lineages-specific genes. 1=expression level in Hopx sufficient cells.

Article Snippet: Total RNA was reverse transcribed and the cDNA was subsequently used for real-time PCR on an ABI Prism instrument using commercial primer-probe sets (Applied Biosystems): HOPX- Mm01217753_m1, ICOS- Mm00497600_m1, Nrp1- Mm00435379_m1, CTLA-4- Mm00486849_m1, GITR- Mm00437136_m1, Helios-Mm00496108_m1, Foxp3- Mm00475165_m1, Fos- Mm00487425_m1, Jun- Mm00495062_s1, Ccnd1- Mm00432359_m1, IL12a(p35)- Mm00434165_m1, Il-10-Mm00439616_m1, Tgfb1- Mm00441724_m1, Hypoxanthineguanine phosphoribosyltransferase (HPRT) was included as an internal control.

Techniques: Expressing, Isolation, FACS, Gene Expression

Journal: iScience

Article Title: Human skin CD141 + dendritic cells regulate cutaneous immunity via the neuropeptide urocortin 2

doi: 10.1016/j.isci.2023.108029

Figure Lengend Snippet:

Article Snippet: Monoclonal anti-Human CD278 (ICOS)-VioGreen (Clone: REA192) , Miltenyi Biotec , Cat # 130-100-740.

Techniques: Purification, Recombinant, Staining, Concentration Assay, Sterility, Reverse Transcription, Multiplex Assay, Labeling, Expressing, Transfection, Software

Journal: Journal of Immunology Research

Article Title: Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

doi: 10.1155/2019/6705949

Figure Lengend Snippet: Panel of antibodies for mass cytometry.

Article Snippet: 25 , CD278 (ICOS) , 143Nd , DX29 , Surface , Fluidigm.

Techniques: Cytometry, Staining