Journal: bioRxiv

Article Title: KCNQ2/3 regulates efferent mediated slow excitation of vestibular afferents in mammals

doi: 10.1101/2023.12.30.573731

Figure Lengend Snippet: A, Continuous rate histograms of regular afferents (CV*<0.1). D, and irregular firing afferents (CV*>0.1). Continuous rate histogram shows changes in afferent firing rate (AFR) during midline efferent stimulation (green bars, 333/s for 5s every 60s). B, E, Individual traces of continuous response histogram of baseline firing rate of regular (CV*<0.1, B) and irregular (CV*>0.1, E) firing afferents post administration of ICA110381, KCNQ2/3 opener, at 5mg/kg. C, F, Baseline amplitudes are plotted for the regular (C) and irregular (F) afferents in the panel as pre– and post-ICA110381 administration. G, H. Mean slow excitation, averaged from a 1-s block at t = 6–7 s, post administration of ICA110381. G, Peak enhancement (p) in slow excitation is aligned for n=8/10 regular units (blue, individual traces; black, average of 8 regular units). G, Slow response amplitude are plotted for all the regular (H) units pre– and post-ICA110381 administration (dark blue, unit enhancement in slow response and p-value for traces only with enhancement post-drug (8/10); light blue lines, regular units with no enhancement in slow response amplitude, p-value for all the regular unit, n=10/10) (student’s paired t-test). I, Slow response trace post administration of ICA110381 for all irregular units (n=5) (blue trace, individual lines; black trace, average trace). J, Slow response amplitude are plotted for all the irregular units pre– and post-ICA110381 administration (student’s paired t-test).

Article Snippet: XE991, E-4031, ICA110381, UCL2077, ML213 and Oxotremorine-M were obtained from Tocris (Tocris Biosciences, Bio-Techne, UK).

Techniques: Blocking Assay

Journal: Communications Biology

Article Title: Permethrin induces epileptic susceptibility via activation of Na + channels and rise in glutamate

doi: 10.1038/s42003-025-09037-0

Figure Lengend Snippet: a qRT-PCR analysis of VGSC gene expression levels in the hippocampus of control and permethrin treated mice. Each dot represents an independent experiment. β-actin is an internal control. b and c Fluorescent images ( b ) and quantitative histogram ( c ) of Nav1.6 (Red) in the CA3 region of the hippocampus in control and PM-treated mice. Scale bar, 20 μm (Control: n = 12, 30% LD 50 : n = 11, 50% LD 50 : n = 12, from 4 mice). d qRT-PCR analysis of Scn8a expression in the hippocampus of control mice and mice treated with ICA-121431 or SYM2206. e Schematic diagram of the PTZ-induced acute seizure model following VGSC inhibitor and permethrin administration. The illustration was created in BioRender. Ming, S. (2025) https://BioRender.com/udftcpr . f Threshold time to generalized seizures in control and chemically treated mice after PTZ injection (Control: n = 8, PM: n = 7, PM + ICA-121431: n = 7, PM + SYM2206: n = 8). g Cumulative number of generalized epileptic seizures in each group of mice after acute PTZ injection. h Current density versus voltage relationship for pyramidal neuron in the hippocampus of mice. Currents were normalized to cell capacitance. i Quantitative histogram of the peak current density (Control: n = 5, PM: n = 7, SYM2206: n = 11, PM + SYM2206: n = 9). j Voltage-dependent steady-state activation curve. The lines are the best-fitted Boltzmann curves. k Quantitative histogram of the V ₁/₂ values from the steady-state activation curve. l Voltage-dependent steady-state inactivation curve. The lines are the best-fitted Boltzmann curves. m Quantitative histogram of the V ₁/₂ values from the steady-state inactivation curve. Statistical significance for a was analyzed using an unpaired one-tailed Student’s t -test; c , d , f , i , k , and m were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test; g was analyzed using two-way ANOVA with Bonferroni’s multiple comparisons test. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001, ns not significant.

Article Snippet: To investigate the involvement of sodium channels in permethrin-induced epileptogenic susceptibility, the sodium channel blocker ICA-121431 (MCE, #HY-16787) and SYM2206 were administered intragastrically at doses of 100 and 24 μM, respectively , .

Techniques: Quantitative RT-PCR, Gene Expression, Control, Expressing, Injection, Activation Assay, One-tailed Test